Restriction map for
pF1KSDA1542
(Circular) (Six-base) MAPSORT of: pF1KSDA1542.seq Check: 5845 from: 1 to: 8084
pF1KSDA1542 8084 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 199 199
Size: 8084
AatII G_ACGT'C
Cuts at: 3391 3391
Size: 8084
AccI GT'mk_AC
Cuts at: 2440 2612 5122 2440
Size: 172 2510 5402
Fragments arranged by size:
5402 2510 172
Acc65I G'GTAC_C
Cuts at: 746 5100 746
Size: 4354 3730
AclI AA'CG_TT
Cuts at: 7924 7924
Size: 8084
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 1980 2146 4060 4618 5909 6341 7088 7356
Size: 1606 166 1914 558 1291 432 747 268
Cuts at: 7356 374
Size: 1102
Fragments arranged by size:
1914 1606 1291 1102 747 558 432 268
166
AflIII A'CryG_T
Cuts at: 2519 3804 3930 5436 6540 7517 2519
Size: 1285 126 1506 1104 977 3086
Fragments arranged by size:
3086 1506 1285 1104 977 126
AgeI A'CCGG_T
Cuts at: 6472 6472
Size: 8084
AleI CACnn'nnGTG
Cuts at: 1093 1093
Size: 8084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 462 2125 2350 4847 4939 462
Size: 1663 225 2497 92 3607
Fragments arranged by size:
3607 2497 1663 225 92
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 784 816 982 1014 4501 4502 4533 4534 784
Size: 32 166 32 3487 1 31 1 4334
Fragments arranged by size:
4334 3487 166 32 32 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 1343 1633 1948 2015 2252 2854 2907 4677 5389
Size: 290 315 67 237 602 53 1770 712
Cuts at: 5389 6956 1343
Size: 1567 2471
Fragments arranged by size:
2471 1770 1567 712 602 315 290 237
67 53
ApaI G_GGCC'C
Cuts at: 3367 3367
Size: 8084
ApaLI G'TGCA_C
Cuts at: 2353 6854 2353
Size: 4501 3583
ApoI r'AATT_y
Cuts at: 5088 7437 7693 5088
Size: 2349 256 5479
Fragments arranged by size:
5479 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8084
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8084
AvaI C'yCGr_G
Cuts at: 920 935 1036 1808 2339 2347 2665 3213 3479
Size: 15 101 772 531 8 318 548 266
Cuts at: 3479 3590 4388 4418 4513 5104 920
Size: 111 798 30 95 591 3900
Fragments arranged by size:
3900 798 772 591 548 531 318 266
111 101 95 30 15 8
BamHI G'GATC_C
Cuts at: 1446 5109 1446
Size: 3663 4421
BanI G'GyrC_C
Cuts at: 746 783 894 1031 1583 1599 1917 2026 2109
Size: 37 111 137 552 16 318 109 83
Cuts at: 2109 2281 2334 4144 4194 4215 4392 5100 5514
Size: 172 53 1810 50 21 177 708 414
Cuts at: 5514 5762 5797 746
Size: 248 35 3033
Fragments arranged by size:
3033 1810 708 552 414 318 248 177
172 137 111 109 83 53 50 37
35 21 16
BanII G_rGCy'C
Cuts at: 166 603 1216 1376 1515 1831 1874 2348 2801
Size: 437 613 160 139 316 43 474 453
Cuts at: 2801 2891 2945 3086 3214 3367 3412 3988 4320
Size: 90 54 141 128 153 45 576 332
Cuts at: 4320 4386 5098 6128 166
Size: 66 712 1030 2122
Fragments arranged by size:
2122 1030 712 613 576 474 453 437
332 316 160 153 141 139 128 90
66 54 45 43
BbeI G_GCGC'C
Cuts at: 1035 1587 1921 4219 5518 5766 1035
Size: 552 334 2298 1299 248 3353
Fragments arranged by size:
3353 2298 1299 552 334 248
BbsI GAAGACnn'nnnn_
Cuts at: 302 326 335 362 1034 1150 1158 2593 3773
Size: 24 9 27 672 116 8 1435 1180
Cuts at: 3773 4118 4238 4762 302
Size: 345 120 524 3624
Fragments arranged by size:
3624 1435 1180 672 524 345 120 116
27 24 9 8
BbvCI CC'TCA_GC
Cuts at: 1717 4190 1717
Size: 2473 5611
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2580 2614 3791 3825 2580
Size: 34 1177 34 6839
Fragments arranged by size:
6839 1177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2162 5982 6743 8084 2162
Size: 3820 761 1341 2162
Fragments arranged by size:
3820 2162 1341 761
BclI T'GATC_A
Cuts at: 5604 5604
Size: 8084
BglI GCCn_nnn'nGGC
Cuts at: 1066 1729 1926 1951 2863 3371 3835 4066 4390
Size: 663 197 25 912 508 464 231 324
Cuts at: 4390 1066
Size: 4760
Fragments arranged by size:
4760 912 663 508 464 324 231 197
25
BglII A'GATC_T
Cuts at: 334 1225 2069 4931 5599 7636 334
Size: 891 844 2862 668 2037 782
Fragments arranged by size:
2862 2037 891 844 782 668
BlpI GC'TnA_GC
Cuts at: 1215 1873 5196 1215
Size: 658 3323 4103
Fragments arranged by size:
4103 3323 658
Bme1580I G_kGCm'C
Cuts at: 507 899 2284 2339 2357 3367 3642 4147 4395
Size: 392 1385 55 18 1010 275 505 248
Cuts at: 4395 4691 5709 5802 6858 507
Size: 296 1018 93 1056 1733
Fragments arranged by size:
1733 1385 1056 1018 1010 505 392 296
275 248 93 55 18
BmgBI CAC'GTC
Cuts at: 3051 3933 3051
Size: 882 7202
BmrI ACTGGGnnnn_n'
Cuts at: 628 2240 2419 4193 4487 4678 5296 5377 5712
Size: 1612 179 1774 294 191 618 81 335
Cuts at: 5712 7856 628
Size: 2144 856
Fragments arranged by size:
2144 1774 1612 856 618 335 294 191
179 81
BmtI G_CTAG'C
Cuts at: 4777 4777
Size: 8084
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 888 1529 1751 2580 2883 6451 888
Size: 641 222 829 303 3568 2521
Fragments arranged by size:
3568 2521 829 641 303 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 760 1721 2484 2628 3273 3285 3532 3883
Size: 363 961 763 144 645 12 247 351
Cuts at: 3883 397
Size: 4598
Fragments arranged by size:
4598 961 763 645 363 351 247 144
12
Bpu10I CC'TnA_GC
Cuts at: 1084 1717 3085 4165 4190 1084
Size: 633 1368 1080 25 4978
Fragments arranged by size:
4978 1368 1080 633 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2058 3933 4566 5260 6139 6631 6929 2058
Size: 1875 633 694 879 492 298 3213
Fragments arranged by size:
3213 1875 879 694 633 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 467 876 3587 7788 467
Size: 409 2711 4201 763
Fragments arranged by size:
4201 2711 763 409
BsaAI yAC'GTr
Cuts at: 4500 4995 5076 5437 4500
Size: 495 81 361 7147
Fragments arranged by size:
7147 495 361 81
BsaBI GATnn'nnATC
Cuts at: 6 5598 7635 6
Size: 5592 2037 455
Fragments arranged by size:
5592 2037 455
BsaHI Gr'CG_yC
Cuts at: 307 490 1032 1114 1584 1918 2965 3388 3956
Size: 183 542 82 470 334 1047 423 568
Cuts at: 3956 4216 5515 5763 6465 7961 307
Size: 260 1299 248 702 1496 430
Fragments arranged by size:
1496 1299 1047 702 568 542 470 430
423 334 260 248 183 82
BsaWI w'CCGG_w
Cuts at: 986 992 1189 2456 3596 4506 5273 5794 6472
Size: 6 197 1267 1140 910 767 521 678
Cuts at: 6472 6746 6893 7304 986
Size: 274 147 411 1766
Fragments arranged by size:
1766 1267 1140 910 767 678 521 411
274 197 147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 690 720 982 1012 690
Size: 30 262 30 7762
Fragments arranged by size:
7762 262 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 673 676 679 877 880 1633 1636 2899
Size: 345 3 3 198 3 753 3 1263
Cuts at: 2899 2968 3007 3256 4294 4765 4813 4855 4909
Size: 69 39 249 1038 471 48 42 54
Cuts at: 4909 5059 328
Size: 150 3353
Fragments arranged by size:
3353 1263 1038 753 471 345 249 198
150 69 54 48 42 39 3 3
3 3
BseYI C'CCAG_C
Cuts at: 1314 1641 1677 2376 2383 3040 3732 4056 4574
Size: 327 36 699 7 657 692 324 518
Cuts at: 4574 4704 5509 6267 6844 7340 1314
Size: 130 805 758 577 496 2058
Fragments arranged by size:
2058 805 758 699 692 657 577 518
496 327 324 130 36 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2764 2867 4801 4945 2764
Size: 103 1934 144 5903
Fragments arranged by size:
5903 1934 144 103
BsiEI CG_ry'CG
Cuts at: 104 705 3397 3403 3830 5626 5672 6880 104
Size: 601 2692 6 427 1796 46 1208 1308
Fragments arranged by size:
2692 1796 1308 1208 601 427 46 6
BsiHKAI G_wGCw'C
Cuts at: 603 1090 1216 1815 1867 2357 3086 3167 3214
Size: 487 126 599 52 490 729 81 47
Cuts at: 3214 3356 3434 3496 3635 3650 3988 5098 5876
Size: 142 78 62 139 15 338 1110 778
Cuts at: 5876 6066 6858 603
Size: 190 792 1829
Fragments arranged by size:
1829 1110 792 778 729 599 490 487
338 190 142 139 126 81 78 62
52 47 15
BsiWI C'GTAC_G
Cuts at: 4991 4991
Size: 8084
BsmI GAATG_Cn'
Cuts at: 466 552 763 466
Size: 86 211 7787
Fragments arranged by size:
7787 211 86
BsmBI CGTCTCn'nnnn_
Cuts at: 1500 1566 1500
Size: 66 8018
Bsp1286I G_dGCh'C
Cuts at: 166 507 603 899 1090 1216 1376 1515 1815
Size: 341 96 296 191 126 160 139 300
Cuts at: 1815 1831 1867 1874 2284 2339 2348 2357 2801
Size: 16 36 7 410 55 9 9 444
Cuts at: 2801 2891 2945 3086 3167 3214 3356 3367 3412
Size: 90 54 141 81 47 142 11 45
Cuts at: 3412 3434 3496 3635 3642 3650 3988 4147 4320
Size: 22 62 139 7 8 338 159 173
Cuts at: 4320 4386 4395 4691 5098 5709 5802 5876 6066
Size: 66 9 296 407 611 93 74 190
Cuts at: 6066 6128 6858 166
Size: 62 730 1392
Fragments arranged by size:
1392 730 611 444 410 407 341 338
300 296 296 191 190 173 160 159
142 141 139 139 126 96 93 90
81 74 66 62 62 55 54 47
45 36 22 16 11 9 9 9
8 7 7
BspEI T'CCGG_A
Cuts at: 1189 2456 4506 7304 1189
Size: 1267 2050 2798 1969
Fragments arranged by size:
2798 2050 1969 1267
BspHI T'CATG_A
Cuts at: 7260 7260
Size: 8084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 199 916 1646 1877 4352 5134 5348 5650 6031
Size: 717 730 231 2475 782 214 302 381
Cuts at: 6031 199
Size: 2252
Fragments arranged by size:
2475 2252 782 730 717 381 302 231
214
BsrBI CCG'CTC
Cuts at: 50 1396 2997 3107 3299 3558 6376 6430 7914
Size: 1346 1601 110 192 259 2818 54 1484
Cuts at: 7914 8079 50
Size: 165 55
Fragments arranged by size:
2818 1601 1484 1346 259 192 165 110
55 54
BsrDI GCAATG_nn'
Cuts at: 370 5996 370
Size: 5626 2458
BsrFI r'CCGG_y
Cuts at: 786 2632 3278 6082 6263 6472 786
Size: 1846 646 2804 181 209 2398
Fragments arranged by size:
2804 2398 1846 646 209 181
BssSI C'ACGA_G
Cuts at: 3553 6355 6713 3553
Size: 2802 358 4924
Fragments arranged by size:
4924 2802 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2043 2751 2854 5461 2043
Size: 708 103 2607 4666
Fragments arranged by size:
4666 2607 708 103
BstBI TT'CG_AA
Cuts at: 110 6445 110
Size: 6335 1749
BstEII G'GTnAC_C
Cuts at: 3003 4332 4884 3003
Size: 1329 552 6203
Fragments arranged by size:
6203 1329 552
BstXI CCAn_nnnn'nTGG
Cuts at: 2945 3510 4320 2945
Size: 565 810 6709
Fragments arranged by size:
6709 810 565
BstYI r'GATC_y
Cuts at: 1 334 629 995 1225 1431 1446 2069 2240
Size: 333 295 366 230 206 15 623 171
Cuts at: 2240 4931 5109 5576 5599 5934 6180 7192 7278
Size: 2691 178 467 23 335 246 1012 86
Cuts at: 7278 7290 7636 1
Size: 12 346 449
Fragments arranged by size:
2691 1012 623 467 449 366 346 335
333 295 246 230 206 178 171 86
23 15 12
Bsu36I CC'TnA_GG
Cuts at: 4277 4277
Size: 8084
BtgI C'CryG_G
Cuts at: 125 482 1001 1547 1565 1595 1910 2223 2391
Size: 357 519 546 18 30 315 313 168
Cuts at: 2391 3917 4070 4616 125
Size: 1526 153 546 3593
Fragments arranged by size:
3593 1526 546 546 519 357 315 313
168 153 30 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 752 6034 6215 6378 752
Size: 5282 181 163 2458
Fragments arranged by size:
5282 2458 181 163
BtsI GCAGTG_nn'
Cuts at: 246 1088 1467 1692 1813 2109 2855 4730 5327
Size: 842 379 225 121 296 746 1875 597
Cuts at: 5327 246
Size: 3003
Fragments arranged by size:
3003 1875 842 746 597 379 296 225
121
DraI TTT'AAA
Cuts at: 5083 5083
Size: 8084
DrdI GACnn_nn'nnGTC
Cuts at: 529 3393 5790 6648 529
Size: 2864 2397 858 1965
Fragments arranged by size:
2864 2397 1965 858
EaeI y'GGCC_r
Cuts at: 1004 1550 3559 3676 3827 4067 4073 4853 4973
Size: 546 2009 117 151 240 6 780 120
Cuts at: 4973 5669 5843 6234 6261 7606 1004
Size: 696 174 391 27 1345 1482
Fragments arranged by size:
2009 1482 1345 780 696 546 391 240
174 151 120 117 27 6
EagI C'GGCC_G
Cuts at: 3827 5669 3827
Size: 1842 6242
EarI CTCTTCn'nnn_
Cuts at: 1132 1249 1254 1460 2578 3142 3426 4020 4870
Size: 117 5 206 1118 564 284 594 850
Cuts at: 4870 6107 6317 1132
Size: 1237 210 2899
Fragments arranged by size:
2899 1237 1118 850 594 564 284 210
206 117 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2814 6292 6602 6748 7894 2814
Size: 3478 310 146 1146 3004
Fragments arranged by size:
3478 3004 1146 310 146
EcoICRI GAG'CTC
Cuts at: 601 1214 3084 3212 3986 5096 601
Size: 613 1870 128 774 1110 3589
Fragments arranged by size:
3589 1870 1110 774 613 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 374 397 760 1721 1980 2146 2484 2628 3273
Size: 23 363 961 259 166 338 144 645
Cuts at: 3273 3285 3532 3883 4060 4618 5909 6341 7088
Size: 12 247 351 177 558 1291 432 747
Cuts at: 7088 7356 374
Size: 268 1102
Fragments arranged by size:
1291 1102 961 747 645 558 432 363
351 338 268 259 247 177 166 144
23 12
EcoNI CCTnn'n_nnAGG
Cuts at: 923 3745 4138 4915 4984 923
Size: 2822 393 777 69 4023
Fragments arranged by size:
4023 2822 777 393 69
EcoO109I rG'GnC_Cy
Cuts at: 669 850 932 1889 2116 2864 2904 3089 3363
Size: 181 82 957 227 748 40 185 274
Cuts at: 3363 3364 3611 3837 5223 669
Size: 1 247 226 1386 3530
Fragments arranged by size:
3530 1386 957 748 274 247 227 226
185 181 82 40 1
EcoRI G'AATT_C
Cuts at: 5088 5088
Size: 8084
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2058 6103 2058
Size: 4045 4039
FspI TGC'GCA
Cuts at: 1324 4599 4915 5017 5865 1324
Size: 3275 316 102 848 3543
Fragments arranged by size:
3543 3275 848 316 102
HaeII r_GCGC'y
Cuts at: 1035 1107 1587 1724 1921 2137 2651 3249 3739
Size: 72 480 137 197 216 514 598 490
Cuts at: 3739 4219 4367 5518 5766 6788 7767 1035
Size: 480 148 1151 248 1022 979 1352
Fragments arranged by size:
1352 1151 1022 979 598 514 490 480
480 248 216 197 148 137 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 292 324 2950 2982 3196 3228 3931 3963 3970
Size: 32 2626 32 214 32 703 32 7
Cuts at: 3970 4002 4225 4257 5926 5956 5958 5988 292
Size: 32 223 32 1669 30 2 30 2388
Fragments arranged by size:
2626 2388 1669 703 223 214 32 32
32 32 32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 2441 5123 2441
Size: 2682 5402
KasI G'GCGC_C
Cuts at: 1031 1583 1917 4215 5514 5762 1031
Size: 552 334 2298 1299 248 3353
Fragments arranged by size:
3353 2298 1299 552 334 248
KpnI G_GTAC'C
Cuts at: 750 5104 750
Size: 4354 3730
MluI A'CGCG_T
Cuts at: 7517 7517
Size: 8084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2648 2663 4334 6755 6939 2648
Size: 15 1671 2421 184 3793
Fragments arranged by size:
3793 2421 1671 184 15
MscI TGG'CCA
Cuts at: 1552 4855 4975 5845 1552
Size: 3303 120 870 3791
Fragments arranged by size:
3791 3303 870 120
MslI CAynn'nnrTG
Cuts at: 278 1093 6073 6200 278
Size: 815 4980 127 2162
Fragments arranged by size:
4980 2162 815 127
MspA1I CmG'CkG
Cuts at: 412 841 1343 1597 1877 1912 2598 3261 3540
Size: 429 502 254 280 35 686 663 279
Cuts at: 3540 3874 4693 5195 5509 5869 6882 7127 412
Size: 334 819 502 314 360 1013 245 1369
Fragments arranged by size:
1369 1013 819 686 663 502 502 429
360 334 314 280 279 254 245 35
NaeI GCC'GGC
Cuts at: 3280 6265 3280
Size: 2985 5099
NarI GG'CG_CC
Cuts at: 1032 1584 1918 4216 5515 5763 1032
Size: 552 334 2298 1299 248 3353
Fragments arranged by size:
3353 2298 1299 552 334 248
NcoI C'CATG_G
Cuts at: 125 2391 125
Size: 2266 5818
NgoMIV G'CCGG_C
Cuts at: 3278 6263 3278
Size: 2985 5099
NheI G'CTAG_C
Cuts at: 4773 4773
Size: 8084
NspI r_CATG'y
Cuts at: 3808 4824 5016 5137 5637 6544 3808
Size: 1016 192 121 500 907 5348
Fragments arranged by size:
5348 1016 907 500 192 121
PasI CC'CwG_GG
Cuts at: 166 1948 2945 3368 166
Size: 1782 997 423 4882
Fragments arranged by size:
4882 1782 997 423
PciI A'CATG_T
Cuts at: 3804 6540 3804
Size: 2736 5348
PflMI CCAn_nnn'nTGG
Cuts at: 483 1548 2907 483
Size: 1065 1359 5660
Fragments arranged by size:
5660 1359 1065
PfoI T'CCnGG_A
Cuts at: 776 1576 1822 2469 3860 776
Size: 800 246 647 1391 5000
Fragments arranged by size:
5000 1391 800 647 246
PmeI GTTT'AAAC
Cuts at: 5083 5083
Size: 8084
PmlI CAC'GTG
Cuts at: 4500 4500
Size: 8084
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 982 1014 2795 2827 7223 7255 982
Size: 32 1781 32 4396 32 1811
Fragments arranged by size:
4396 1811 1781 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 669 850 932 1889 3611 669
Size: 181 82 957 1722 5142
Fragments arranged by size:
5142 1722 957 181 82
PspOMI G'GGCC_C
Cuts at: 3363 3363
Size: 8084
PspXI vC'TCGA_Gb
Cuts at: 920 3213 920
Size: 2293 5791
PstI C_TGCA'G
Cuts at: 1535 1805 3059 3526 3848 4349 4737 5131 5816
Size: 270 1254 467 322 501 388 394 685
Cuts at: 5816 1535
Size: 3803
Fragments arranged by size:
3803 1254 685 501 467 394 388 322
270
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8084
PvuII CAG'CTG
Cuts at: 1343 1877 2598 3874 5509 5869 1343
Size: 534 721 1276 1635 360 3558
Fragments arranged by size:
3558 1635 1276 721 534 360
RsrII CG'GwC_CG
Cuts at: 2815 6279 2815
Size: 3464 4620
SacI G_AGCT'C
Cuts at: 603 1216 3086 3214 3988 5098 603
Size: 613 1870 128 774 1110 3589
Fragments arranged by size:
3589 1870 1110 774 613 128
SacII CC_GC'GG
Cuts at: 1598 1913 1598
Size: 315 7769
SalI G'TCGA_C
Cuts at: 2439 5121 2439
Size: 2682 5402
SanDI GG'GwC_CC
Cuts at: 850 850
Size: 8084
SapI GCTCTTCn'nnn_
Cuts at: 1460 3426 6107 6317 1460
Size: 1966 2681 210 3227
Fragments arranged by size:
3227 2681 1966 210
SbfI CC_TGCA'GG
Cuts at: 3848 4349 5131 3848
Size: 501 782 6801
Fragments arranged by size:
6801 782 501
ScaI AGT'ACT
Cuts at: 7601 7601
Size: 8084
SexAI A'CCwGG_T
Cuts at: 3778 4979 3778
Size: 1201 6883
SfcI C'TryA_G
Cuts at: 33 1531 1801 3055 3522 3844 4345 4733 5127
Size: 1498 270 1254 467 322 501 388 394
Cuts at: 5127 5812 6805 6996 33
Size: 685 993 191 1121
Fragments arranged by size:
1498 1254 1121 993 685 501 467 394
388 322 270 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3371 3835 3371
Size: 464 7620
SfoI GGC'GCC
Cuts at: 1033 1585 1919 4217 5516 5764 1033
Size: 552 334 2298 1299 248 3353
Fragments arranged by size:
3353 2298 1299 552 334 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8084
SmaI CCC'GGG
Cuts at: 937 2341 2349 4390 4515 5106 937
Size: 1404 8 2041 125 591 3915
Fragments arranged by size:
3915 2041 1404 591 125 8
SmlI C'TyrA_G
Cuts at: 920 2073 3213 3948 4581 5239 6154 6646 6908
Size: 1153 1140 735 633 658 915 492 262
Cuts at: 6908 920
Size: 2096
Fragments arranged by size:
2096 1153 1140 915 735 658 633 492
262
SnaBI TAC'GTA
Cuts at: 5076 5076
Size: 8084
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8084
SphI G_CATG'C
Cuts at: 5137 5637 5137
Size: 500 7584
SspI AAT'ATT
Cuts at: 2466 2466
Size: 8084
StuI AGG'CCT
Cuts at: 1329 1329
Size: 8084
StyI C'CwwG_G
Cuts at: 125 1836 2391 3266 3335 3791 4889 5218 5572
Size: 1711 555 875 69 456 1098 329 354
Cuts at: 5572 125
Size: 2637
Fragments arranged by size:
2637 1711 1098 875 555 456 354 329
69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 197 3054 3082 3226 3650 4162 4212 5667 5800
Size: 2857 28 144 424 512 50 1455 133
Cuts at: 5800 6259 6468 197
Size: 459 209 1813
Fragments arranged by size:
2857 1813 1455 512 459 424 209 144
133 50 28
TatI w'GTAC_w
Cuts at: 4802 4898 5003 7599 4802
Size: 96 105 2596 5287
Fragments arranged by size:
5287 2596 105 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 690 722 3561 3593 7601 7633 690
Size: 32 2839 32 4008 32 1141
Fragments arranged by size:
4008 2839 1141 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3687 5881 3687
Size: 2194 5890
XbaI T'CTAG_A
Cuts at: 5115 5115
Size: 8084
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1068 3507 4068 4945 5579 1068
Size: 2439 561 877 634 3573
Fragments arranged by size:
3573 2439 877 634 561
XhoI C'TCGA_G
Cuts at: 920 3213 920
Size: 2293 5791
XmaI C'CCGG_G
Cuts at: 935 2339 2347 4388 4513 5104 935
Size: 1404 8 2041 125 591 3915
Fragments arranged by size:
3915 2041 1404 591 125 8
ZraI GAC'GTC
Cuts at: 3389 3389
Size: 8084
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BfrBI BsrGI
BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PshAI PsiI PsrI SgrAI SrfI SwaI XmnI