Restriction map for
pF1KSDA1547
(Circular) (Six-base) MAPSORT of: pF1KSDA1547.seq Check: 1345 from: 1 to: 5417
pF1KSDA1547 5417 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 423 2189 423
Size: 1766 3651
AatII G_ACGT'C
Cuts at: 1810 1810
Size: 5417
AccI GT'mk_AC
Cuts at: 1592 2455 1592
Size: 863 4554
Acc65I G'GTAC_C
Cuts at: 2433 2433
Size: 5417
AclI AA'CG_TT
Cuts at: 5257 5257
Size: 5417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3242 3674 4421 4689 3242
Size: 432 747 268 3970
Fragments arranged by size:
3970 747 432 268
AflIII A'CryG_T
Cuts at: 2769 3873 4850 2769
Size: 1104 977 3336
Fragments arranged by size:
3336 1104 977
AgeI A'CCGG_T
Cuts at: 3805 3805
Size: 5417
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 114 146 114
Size: 32 5385
AlwNI CAG_nnn'CTG
Cuts at: 231 1312 1917 2368 2722 4289 231
Size: 1081 605 451 354 1567 1359
Fragments arranged by size:
1567 1359 1081 605 451 354
ApaLI G'TGCA_C
Cuts at: 4187 4187
Size: 5417
ApoI r'AATT_y
Cuts at: 181 220 2421 4770 5026 181
Size: 39 2201 2349 256 572
Fragments arranged by size:
2349 2201 572 256 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 5417
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5417
AvaI C'yCGr_G
Cuts at: 163 905 1509 2437 163
Size: 742 604 928 3143
Fragments arranged by size:
3143 928 742 604
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 828 861 828
Size: 33 5384
BamHI G'GATC_C
Cuts at: 2442 2442
Size: 5417
BanI G'GyrC_C
Cuts at: 634 1114 1194 1249 1978 2433 2847 3095 3130
Size: 480 80 55 729 455 414 248 35
Cuts at: 3130 634
Size: 2921
Fragments arranged by size:
2921 729 480 455 414 248 80 55
35
BanII G_rGCy'C
Cuts at: 775 1070 1136 1654 2431 3461 775
Size: 295 66 518 777 1030 2731
Fragments arranged by size:
2731 1030 777 518 295 66
BbeI G_GCGC'C
Cuts at: 638 1198 1253 2851 3099 638
Size: 560 55 1598 248 2956
Fragments arranged by size:
2956 1598 560 248 55
BbsI GAAGACnn'nnnn_
Cuts at: 292 292
Size: 5417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1713 1747 2214 2248 1713
Size: 34 467 34 4882
Fragments arranged by size:
4882 467 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 140 3315 4076 5417 140
Size: 3175 761 1341 140
Fragments arranged by size:
3175 1341 761 140
BclI T'GATC_A
Cuts at: 2937 2937
Size: 5417
BglI GCCn_nnn'nGGC
Cuts at: 994 1640 994
Size: 646 4771
BglII A'GATC_T
Cuts at: 1619 2072 2932 4969 1619
Size: 453 860 2037 2067
Fragments arranged by size:
2067 2037 860 453
BlpI GC'TnA_GC
Cuts at: 2529 2529
Size: 5417
Bme1580I G_kGCm'C
Cuts at: 1475 3042 3135 4191 1475
Size: 1567 93 1056 2701
Fragments arranged by size:
2701 1567 1056 93
BmgBI CAC'GTC
Cuts at: 1590 1590
Size: 5417
BmrI ACTGGGnnnn_n'
Cuts at: 592 640 2108 2252 2629 2710 3045 5189 592
Size: 48 1468 144 377 81 335 2144 820
Fragments arranged by size:
2144 1468 820 377 335 144 81 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3784 3784
Size: 5417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 562 569 1094 1436 2289 493
Size: 69 7 525 342 853 3621
Fragments arranged by size:
3621 853 525 342 69 7
Bpu10I CC'TnA_GC
Cuts at: 1135 1135
Size: 5417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 849 1054 1380 2191 2343 2593 3472 3964 4262
Size: 205 326 811 152 250 879 492 298
Cuts at: 4262 849
Size: 2004
Fragments arranged by size:
2004 879 811 492 326 298 250 205
152
BsaI GGTCTCn'nnnn_
Cuts at: 580 5121 580
Size: 4541 876
BsaAI yAC'GTr
Cuts at: 2409 2770 2409
Size: 361 5056
BsaBI GATnn'nnATC
Cuts at: 6 2931 4968 6
Size: 2925 2037 455
Fragments arranged by size:
2925 2037 455
BsaHI Gr'CG_yC
Cuts at: 635 1100 1195 1250 1487 1807 1850 2275 2848
Size: 465 95 55 237 320 43 425 573
Cuts at: 2848 3096 3798 5294 635
Size: 248 702 1496 758
Fragments arranged by size:
1496 758 702 573 465 425 320 248
237 95 55 43
BsaWI w'CCGG_w
Cuts at: 2606 3127 3805 4079 4226 4637 2606
Size: 521 678 274 147 411 3386
Fragments arranged by size:
3386 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1039 1069 1540 1570 1681 1711 1039
Size: 30 471 30 111 30 4745
Fragments arranged by size:
4745 471 111 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 829
Size: 5417
BseYI C'CCAG_C
Cuts at: 1661 2082 2842 3600 4177 4673 1661
Size: 421 760 758 577 496 2405
Fragments arranged by size:
2405 760 758 577 496 421
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1888 2131 2161 2167 1888
Size: 243 30 6 5138
Fragments arranged by size:
5138 243 30 6
BsiEI CG_ry'CG
Cuts at: 104 1327 2959 3005 4213 104
Size: 1223 1632 46 1208 1308
Fragments arranged by size:
1632 1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 425 2201 2242 2431 3209 3399 4191 425
Size: 1776 41 189 778 190 792 1651
Fragments arranged by size:
1776 1651 792 778 190 189 41
Bsp1286I G_dGCh'C
Cuts at: 425 775 1070 1136 1475 1654 2201 2242 2431
Size: 350 295 66 339 179 547 41 189
Cuts at: 2431 3042 3135 3209 3399 3461 4191 425
Size: 611 93 74 190 62 730 1651
Fragments arranged by size:
1651 730 611 547 350 339 295 190
189 179 93 74 66 62 41
BspEI T'CCGG_A
Cuts at: 4637 4637
Size: 5417
BspHI T'CATG_A
Cuts at: 4593 4593
Size: 5417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 423 453 1910 2036 2189 2467 2681 2983 3364
Size: 30 1457 126 153 278 214 302 381
Cuts at: 3364 423
Size: 2476
Fragments arranged by size:
2476 1457 381 302 278 214 153 126
30
BsrBI CCG'CTC
Cuts at: 50 1697 3709 3763 5247 5412 50
Size: 1647 2012 54 1484 165 55
Fragments arranged by size:
2012 1647 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1886 3329 1886
Size: 1443 3974
BsrFI r'CCGG_y
Cuts at: 1146 1258 3415 3596 3805 1146
Size: 112 2157 181 209 2758
Fragments arranged by size:
2758 2157 209 181 112
BssHII G'CGCG_C
Cuts at: 453 453
Size: 5417
BssSI C'ACGA_G
Cuts at: 1581 2185 3688 4046 1581
Size: 604 1503 358 2952
Fragments arranged by size:
2952 1503 604 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1312 1470 1610 2794 1312
Size: 158 140 1184 3935
Fragments arranged by size:
3935 1184 158 140
BstBI TT'CG_AA
Cuts at: 110 3778 110
Size: 3668 1749
BstEII G'GTnAC_C
Cuts at: 465 465
Size: 5417
BstXI CCAn_nnnn'nTGG
Cuts at: 1945 1945
Size: 5417
BstYI r'GATC_y
Cuts at: 1 1619 2072 2442 2909 2932 3267 3513 4525
Size: 1618 453 370 467 23 335 246 1012
Cuts at: 4525 4611 4623 4969 1
Size: 86 12 346 449
Fragments arranged by size:
1618 1012 467 453 449 370 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 1542 1542
Size: 5417
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1892 3367 3548 3711 1892
Size: 1475 181 163 3598
Fragments arranged by size:
3598 1475 181 163
BtsI GCAGTG_nn'
Cuts at: 452 2660 452
Size: 2208 3209
DraI TTT'AAA
Cuts at: 180 2416 180
Size: 2236 3181
DrdI GACnn_nn'nnGTC
Cuts at: 1855 3123 3981 1855
Size: 1268 858 3291
Fragments arranged by size:
3291 1268 858
EaeI y'GGCC_r
Cuts at: 1320 1835 3002 3176 3567 3594 4939 1320
Size: 515 1167 174 391 27 1345 1798
Fragments arranged by size:
1798 1345 1167 515 391 174 27
EagI C'GGCC_G
Cuts at: 3002 3002
Size: 5417
EarI CTCTTCn'nnn_
Cuts at: 367 1005 3440 3650 367
Size: 638 2435 210 2134
Fragments arranged by size:
2435 2134 638 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 826 3625 3935 4081 5227 826
Size: 2799 310 146 1146 1016
Fragments arranged by size:
2799 1146 1016 310 146
EcoICRI GAG'CTC
Cuts at: 2429 2429
Size: 5417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 562 569 1094 1436 2289 3242 3674 4421
Size: 69 7 525 342 853 953 432 747
Cuts at: 4421 4689 493
Size: 268 1221
Fragments arranged by size:
1221 953 853 747 525 432 342 268
69 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2032 2032
Size: 5417
EcoO109I rG'GnC_Cy
Cuts at: 891 1498 1767 1899 1998 2025 2556 891
Size: 607 269 132 99 27 531 3752
Fragments arranged by size:
3752 607 531 269 132 99 27
EcoRI G'AATT_C
Cuts at: 220 2421 220
Size: 2201 3216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 843 2272 3436 843
Size: 1429 1164 2824
Fragments arranged by size:
2824 1429 1164
FspI TGC'GCA
Cuts at: 1162 3198 1162
Size: 2036 3381
HaeII r_GCGC'y
Cuts at: 303 638 1198 1253 1292 2851 3099 4121 5100
Size: 335 560 55 39 1559 248 1022 979
Cuts at: 5100 303
Size: 620
Fragments arranged by size:
1559 1022 979 620 560 335 248 55
39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 694 726 849 881 1039 1071 1759 1791 1792
Size: 32 123 32 158 32 688 32 1
Cuts at: 1792 1824 3259 3289 3291 3321 694
Size: 32 1435 30 2 30 2790
Fragments arranged by size:
2790 1435 688 158 123 32 32 32
32 32 30 30 2 1
HincII GTy'rAC
Cuts at: 2456 2456
Size: 5417
KasI G'GCGC_C
Cuts at: 634 1194 1249 2847 3095 634
Size: 560 55 1598 248 2956
Fragments arranged by size:
2956 1598 560 248 55
KpnI G_GTAC'C
Cuts at: 2437 2437
Size: 5417
MluI A'CGCG_T
Cuts at: 4850 4850
Size: 5417
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1100 4088 4272 1100
Size: 2988 184 2245
Fragments arranged by size:
2988 2245 184
MscI TGG'CCA
Cuts at: 1837 3178 1837
Size: 1341 4076
MslI CAynn'nnrTG
Cuts at: 346 1445 1943 3406 3533 346
Size: 1099 498 1463 127 2230
Fragments arranged by size:
2230 1463 1099 498 127
MspA1I CmG'CkG
Cuts at: 1300 1312 1665 1955 2175 2341 2528 2842 3202
Size: 12 353 290 220 166 187 314 360
Cuts at: 3202 4215 4460 1300
Size: 1013 245 2257
Fragments arranged by size:
2257 1013 360 353 314 290 245 220
187 166 12
NaeI GCC'GGC
Cuts at: 1148 1260 3598 1148
Size: 112 2338 2967
Fragments arranged by size:
2967 2338 112
NarI GG'CG_CC
Cuts at: 635 1195 1250 2848 3096 635
Size: 560 55 1598 248 2956
Fragments arranged by size:
2956 1598 560 248 55
NgoMIV G'CCGG_C
Cuts at: 1146 1258 3596 1146
Size: 112 2338 2967
Fragments arranged by size:
2967 2338 112
NspI r_CATG'y
Cuts at: 2470 2970 3877 2470
Size: 500 907 4010
Fragments arranged by size:
4010 907 500
PasI CC'CwG_GG
Cuts at: 639 1063 1129 639
Size: 424 66 4927
Fragments arranged by size:
4927 424 66
PciI A'CATG_T
Cuts at: 3873 3873
Size: 5417
PflMI CCAn_nnn'nTGG
Cuts at: 1917 1988 1917
Size: 71 5346
PfoI T'CCnGG_A
Cuts at: 585 1413 585
Size: 828 4589
PmeI GTTT'AAAC
Cuts at: 2416 2416
Size: 5417
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4556 4588 4556
Size: 32 5385
PpuMI rG'GwC_Cy
Cuts at: 891 1767 1899 2025 891
Size: 876 132 126 4283
Fragments arranged by size:
4283 876 132 126
PshAI GACnn'nnGTC
Cuts at: 1037 1918 1037
Size: 881 4536
PstI C_TGCA'G
Cuts at: 1308 1876 2464 3149 1308
Size: 568 588 685 3576
Fragments arranged by size:
3576 685 588 568
PvuI CG_AT'CG
Cuts at: 104 1327 104
Size: 1223 4194
PvuII CAG'CTG
Cuts at: 1665 1955 2175 2842 3202 1665
Size: 290 220 667 360 3880
Fragments arranged by size:
3880 667 360 290 220
RsrII CG'GwC_CG
Cuts at: 3612 3612
Size: 5417
SacI G_AGCT'C
Cuts at: 2431 2431
Size: 5417
SalI G'TCGA_C
Cuts at: 2454 2454
Size: 5417
SapI GCTCTTCn'nnn_
Cuts at: 3440 3650 3440
Size: 210 5207
SbfI CC_TGCA'GG
Cuts at: 2464 2464
Size: 5417
ScaI AGT'ACT
Cuts at: 4934 4934
Size: 5417
SfcI C'TryA_G
Cuts at: 33 783 1304 1872 2047 2460 3145 4138 4329
Size: 750 521 568 175 413 685 993 191
Cuts at: 4329 33
Size: 1121
Fragments arranged by size:
1121 993 750 685 568 521 413 191
175
SfoI GGC'GCC
Cuts at: 636 1196 1251 2849 3097 636
Size: 560 55 1598 248 2956
Fragments arranged by size:
2956 1598 560 248 55
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5417
SmaI CCC'GGG
Cuts at: 165 907 2439 165
Size: 742 1532 3143
Fragments arranged by size:
3143 1532 742
SmlI C'TyrA_G
Cuts at: 828 1069 1395 2206 2322 2572 3487 3979 4241
Size: 241 326 811 116 250 915 492 262
Cuts at: 4241 828
Size: 2004
Fragments arranged by size:
2004 915 811 492 326 262 250 241
116
SnaBI TAC'GTA
Cuts at: 2409 2409
Size: 5417
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5417
SphI G_CATG'C
Cuts at: 2470 2970 2470
Size: 500 4917
StuI AGG'CCT
Cuts at: 1388 1388
Size: 5417
StyI C'CwwG_G
Cuts at: 1035 1090 2551 2905 1035
Size: 55 1461 354 3547
Fragments arranged by size:
3547 1461 354 55
SwaI ATTT'AAAT
Cuts at: 180 180
Size: 5417
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3000 3133 3592 3801 3000
Size: 133 459 209 4616
Fragments arranged by size:
4616 459 209 133
TatI w'GTAC_w
Cuts at: 314 4932 314
Size: 4618 799
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1540 1572 4934 4966 1540
Size: 32 3362 32 1991
Fragments arranged by size:
3362 1991 32 32
Tth111I GACn'n_nGTC
Cuts at: 3214 3214
Size: 5417
XbaI T'CTAG_A
Cuts at: 2448 2448
Size: 5417
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1662 1767 2912 1662
Size: 105 1145 4167
Fragments arranged by size:
4167 1145 105
XmaI C'CCGG_G
Cuts at: 163 905 2437 163
Size: 742 1532 3143
Fragments arranged by size:
3143 1532 742
ZraI GAC'GTC
Cuts at: 1808 1808
Size: 5417
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI ApaI AscI AvrII
BbvCI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI XhoI XmnI