Restriction map for
pF1KSDA1552
(Circular) (Six-base) MAPSORT of: pF1KSDA1552.seq Check: 1317 from: 1 to: 5282
pF1KSDA1552 5282 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 209 2249 209
Size: 2040 3242
AccI GT'mk_AC
Cuts at: 2320 2320
Size: 5282
Acc65I G'GTAC_C
Cuts at: 2298 2298
Size: 5282
AclI AA'CG_TT
Cuts at: 5122 5122
Size: 5282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 1672 3107 3539 4286 4554 1408
Size: 264 1435 432 747 268 2136
Fragments arranged by size:
2136 1435 747 432 268 264
AfeI AGC'GCT
Cuts at: 901 901
Size: 5282
AflIII A'CryG_T
Cuts at: 2203 2634 3738 4715 2203
Size: 431 1104 977 2770
Fragments arranged by size:
2770 1104 977 431
AgeI A'CCGG_T
Cuts at: 3670 3670
Size: 5282
AlwNI CAG_nnn'CTG
Cuts at: 372 462 1317 1817 2090 2587 4154 372
Size: 90 855 500 273 497 1567 1500
Fragments arranged by size:
1567 1500 855 500 497 273 90
ApaI G_GGCC'C
Cuts at: 745 784 745
Size: 39 5243
ApaLI G'TGCA_C
Cuts at: 310 1954 4052 310
Size: 1644 2098 1540
Fragments arranged by size:
2098 1644 1540
ApoI r'AATT_y
Cuts at: 2286 4635 4891 2286
Size: 2349 256 2677
Fragments arranged by size:
2677 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5282
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
AvaI C'yCGr_G
Cuts at: 136 605 805 863 934 1330 1368 1642 2302
Size: 469 200 58 71 396 38 274 660
Cuts at: 2302 136
Size: 3116
Fragments arranged by size:
3116 660 469 396 274 200 71 58
38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 964 997 964
Size: 33 5249
BamHI G'GATC_C
Cuts at: 292 2307 292
Size: 2015 3267
BanI G'GyrC_C
Cuts at: 674 858 2298 2712 2960 2995 674
Size: 184 1440 414 248 35 2961
Fragments arranged by size:
2961 1440 414 248 184 35
BanII G_rGCy'C
Cuts at: 143 728 745 767 784 806 812 870 1633
Size: 585 17 22 17 22 6 58 763
Cuts at: 1633 2296 3326 143
Size: 663 1030 2099
Fragments arranged by size:
2099 1030 763 663 585 58 22 22
17 17 6
BbeI G_GCGC'C
Cuts at: 678 2716 2964 678
Size: 2038 248 2996
Fragments arranged by size:
2996 2038 248
BbsI GAAGACnn'nnnn_
Cuts at: 1313 1396 1660 1313
Size: 83 264 4935
Fragments arranged by size:
4935 264 83
BciVI GTATCCnnnnn_n'
Cuts at: 3180 3941 5282 3180
Size: 761 1341 3180
Fragments arranged by size:
3180 1341 761
BclI T'GATC_A
Cuts at: 2802 2802
Size: 5282
BglI GCCn_nnn'nGGC
Cuts at: 749 1637 749
Size: 888 4394
BglII A'GATC_T
Cuts at: 2797 4834 2797
Size: 2037 3245
BlpI GC'TnA_GC
Cuts at: 575 829 880 2394 575
Size: 254 51 1514 3463
Fragments arranged by size:
3463 1514 254 51
Bme1580I G_kGCm'C
Cuts at: 314 745 784 796 1109 1958 2907 3000 4056
Size: 431 39 12 313 849 949 93 1056
Cuts at: 4056 314
Size: 1540
Fragments arranged by size:
1540 1056 949 849 431 313 93 39
12
BmgBI CAC'GTC
Cuts at: 950 2204 950
Size: 1254 4028
BmrI ACTGGGnnnn_n'
Cuts at: 2262 2494 2575 2910 5054 2262
Size: 232 81 335 2144 2490
Fragments arranged by size:
2490 2144 335 232 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3649 3649
Size: 5282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 484 526 685 767 790 865 1600 1864
Size: 135 42 159 82 23 75 735 264
Cuts at: 1864 1936 2137 349
Size: 72 201 3494
Fragments arranged by size:
3494 735 264 201 159 135 82 75
72 42 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2145 2458 3337 3829 4127 2145
Size: 313 879 492 298 3300
Fragments arranged by size:
3300 879 492 313 298
BsaI GGTCTCn'nnnn_
Cuts at: 4986 4986
Size: 5282
BsaAI yAC'GTr
Cuts at: 2274 2635 2274
Size: 361 4921
BsaBI GATnn'nnATC
Cuts at: 6 2796 4833 6
Size: 2790 2037 455
Fragments arranged by size:
2790 2037 455
BsaHI Gr'CG_yC
Cuts at: 206 675 1393 1657 2246 2713 2961 3663 5159
Size: 469 718 264 589 467 248 702 1496
Cuts at: 5159 206
Size: 329
Fragments arranged by size:
1496 718 702 589 469 467 329 264
248
BsaWI w'CCGG_w
Cuts at: 1827 2471 2992 3670 3944 4091 4502 1827
Size: 644 521 678 274 147 411 2607
Fragments arranged by size:
2607 678 644 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 383 413 740 770 1450 1480 1714 1744 1987
Size: 30 327 30 680 30 234 30 243
Cuts at: 1987 2017 2193 2223 383
Size: 30 176 30 3442
Fragments arranged by size:
3442 680 327 243 234 176 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 775 814 862 865 868 397
Size: 378 39 48 3 3 4811
Fragments arranged by size:
4811 378 48 39 3 3
BseYI C'CCAG_C
Cuts at: 142 579 694 915 1180 1325 1453 1990 2031
Size: 437 115 221 265 145 128 537 41
Cuts at: 2031 2156 2707 3465 4042 4538 142
Size: 125 551 758 577 496 886
Fragments arranged by size:
886 758 577 551 537 496 437 265
221 145 128 125 115 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 1033 453
Size: 580 4702
BsiEI CG_ry'CG
Cuts at: 104 1718 2824 2870 4078 104
Size: 1614 1106 46 1208 1308
Fragments arranged by size:
1614 1308 1208 1106 46
BsiHKAI G_wGCw'C
Cuts at: 314 1349 1868 1958 2296 3074 3264 4056 314
Size: 1035 519 90 338 778 190 792 1540
Fragments arranged by size:
1540 1035 792 778 519 338 190 90
BsmBI CGTCTCn'nnnn_
Cuts at: 1162 1162
Size: 5282
Bsp1286I G_dGCh'C
Cuts at: 143 314 728 745 767 784 796 806 812
Size: 171 414 17 22 17 12 10 6
Cuts at: 812 870 1109 1349 1633 1868 1958 2296 2907
Size: 58 239 240 284 235 90 338 611
Cuts at: 2907 3000 3074 3264 3326 4056 143
Size: 93 74 190 62 730 1369
Fragments arranged by size:
1369 730 611 414 338 284 240 239
235 190 171 93 90 74 62 58
22 17 17 12 10 6
BspEI T'CCGG_A
Cuts at: 4502 4502
Size: 5282
BspHI T'CATG_A
Cuts at: 4458 4458
Size: 5282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1039 1483 1747 1934 2332 2546 2848 3229 1039
Size: 444 264 187 398 214 302 381 3092
Fragments arranged by size:
3092 444 398 381 302 264 214 187
BsrBI CCG'CTC
Cuts at: 50 2139 3574 3628 5112 5277 50
Size: 2089 1435 54 1484 165 55
Fragments arranged by size:
2089 1484 1435 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3194 3194
Size: 5282
BsrFI r'CCGG_y
Cuts at: 172 837 1436 1700 1790 3280 3461 3670 172
Size: 665 599 264 90 1490 181 209 1784
Fragments arranged by size:
1784 1490 665 599 264 209 181 90
BsrGI T'GTAC_A
Cuts at: 518 518
Size: 5282
BssSI C'ACGA_G
Cuts at: 976 1957 3553 3911 976
Size: 981 1596 358 2347
Fragments arranged by size:
2347 1596 981 358
BstAPI GCAn_nnn'nTGC
Cuts at: 372 1499 1763 1817 2090 2659 372
Size: 1127 264 54 273 569 2995
Fragments arranged by size:
2995 1127 569 273 264 54
BstBI TT'CG_AA
Cuts at: 110 3643 110
Size: 3533 1749
BstYI r'GATC_y
Cuts at: 1 292 368 1899 2307 2774 2797 3132 3378
Size: 291 76 1531 408 467 23 335 246
Cuts at: 3378 4390 4476 4488 4834 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1531 1012 467 449 408 346 335 291
246 86 76 23 12
Bsu36I CC'TnA_GG
Cuts at: 472 737 797 943 1588 1924 472
Size: 265 60 146 645 336 3830
Fragments arranged by size:
3830 645 336 265 146 60
BtgI C'CryG_G
Cuts at: 784 1163 2150 784
Size: 379 987 3916
Fragments arranged by size:
3916 987 379
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3232 3413 3576 3232
Size: 181 163 4938
Fragments arranged by size:
4938 181 163
BtsI GCAGTG_nn'
Cuts at: 312 542 636 796 874 1107 1551 1815 1891
Size: 230 94 160 78 233 444 264 76
Cuts at: 1891 2088 2525 312
Size: 197 437 3069
Fragments arranged by size:
3069 444 437 264 233 230 197 160
94 78 76
DraI TTT'AAA
Cuts at: 2281 2281
Size: 5282
DrdI GACnn_nn'nnGTC
Cuts at: 2988 3846 2988
Size: 858 4424
EaeI y'GGCC_r
Cuts at: 174 1715 2867 3041 3432 3459 4804 174
Size: 1541 1152 174 391 27 1345 652
Fragments arranged by size:
1541 1345 1152 652 391 174 27
EagI C'GGCC_G
Cuts at: 1715 2867 1715
Size: 1152 4130
EarI CTCTTCn'nnn_
Cuts at: 3305 3515 3305
Size: 210 5072
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3490 3800 3946 5092 3490
Size: 310 146 1146 3680
Fragments arranged by size:
3680 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2294 2294
Size: 5282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 484 526 685 767 790 865 1408 1600
Size: 135 42 159 82 23 75 543 192
Cuts at: 1600 1672 1864 1936 2137 3107 3539 4286 4554
Size: 72 192 72 201 970 432 747 268
Cuts at: 4554 349
Size: 1077
Fragments arranged by size:
1077 970 747 543 432 268 201 192
192 159 135 82 75 72 72 42
23
EcoNI CCTnn'n_nnAGG
Cuts at: 709 847 709
Size: 138 5144
EcoO109I rG'GnC_Cy
Cuts at: 733 741 742 1131 1258 1335 1375 1584 1639
Size: 8 1 389 127 77 40 209 55
Cuts at: 1639 1848 2166 2421 733
Size: 209 318 255 3594
Fragments arranged by size:
3594 389 318 255 209 209 127 77
55 40 8 1
EcoRI G'AATT_C
Cuts at: 2286 2286
Size: 5282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3301 3301
Size: 5282
FseI GG_CCGG'CC
Cuts at: 176 176
Size: 5282
FspI TGC'GCA
Cuts at: 1810 2083 3063 1810
Size: 273 980 4029
Fragments arranged by size:
4029 980 273
HaeII r_GCGC'y
Cuts at: 678 903 2716 2964 3986 4965 678
Size: 225 1813 248 1022 979 995
Fragments arranged by size:
1813 1022 995 979 248 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 383 415 1286 1318 2193 2225 2248 2280 3124
Size: 32 871 32 875 32 23 32 844
Cuts at: 3124 3154 3156 3186 383
Size: 30 2 30 2479
Fragments arranged by size:
2479 875 871 844 32 32 32 32
30 30 23 2
HincII GTy'rAC
Cuts at: 2321 2321
Size: 5282
KasI G'GCGC_C
Cuts at: 674 2712 2960 674
Size: 2038 248 2996
Fragments arranged by size:
2996 2038 248
KpnI G_GTAC'C
Cuts at: 2302 2302
Size: 5282
MluI A'CGCG_T
Cuts at: 4715 4715
Size: 5282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 293 3953 4137 293
Size: 3660 184 1438
Fragments arranged by size:
3660 1438 184
MscI TGG'CCA
Cuts at: 3043 3043
Size: 5282
MslI CAynn'nnrTG
Cuts at: 625 1804 2077 3271 3398 625
Size: 1179 273 1194 127 2509
Fragments arranged by size:
2509 1194 1179 273 127
MspA1I CmG'CkG
Cuts at: 1086 1596 1623 1860 2190 2393 2707 3067 4080
Size: 510 27 237 330 203 314 360 1013
Cuts at: 4080 4325 1086
Size: 245 2043
Fragments arranged by size:
2043 1013 510 360 330 314 245 237
203 27
NaeI GCC'GGC
Cuts at: 174 839 1792 3463 174
Size: 665 953 1671 1993
Fragments arranged by size:
1993 1671 953 665
NarI GG'CG_CC
Cuts at: 675 2713 2961 675
Size: 2038 248 2996
Fragments arranged by size:
2996 2038 248
NgoMIV G'CCGG_C
Cuts at: 172 837 1790 3461 172
Size: 665 953 1671 1993
Fragments arranged by size:
1993 1671 953 665
NspI r_CATG'y
Cuts at: 574 2335 2835 3742 574
Size: 1761 500 907 2114
Fragments arranged by size:
2114 1761 907 500
PasI CC'CwG_GG
Cuts at: 233 729 1077 1195 1398 1521 1662 1785 1935
Size: 496 348 118 203 123 141 123 150
Cuts at: 1935 2058 233
Size: 123 3457
Fragments arranged by size:
3457 496 348 203 150 141 123 123
123 118
PciI A'CATG_T
Cuts at: 3738 3738
Size: 5282
PflMI CCAn_nnn'nTGG
Cuts at: 1356 1356
Size: 5282
PmeI GTTT'AAAC
Cuts at: 2281 2281
Size: 5282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 4421
Size: 32 5250
PpuMI rG'GwC_Cy
Cuts at: 733 1258 2166 733
Size: 525 908 3849
Fragments arranged by size:
3849 908 525
PspOMI G'GGCC_C
Cuts at: 741 780 741
Size: 39 5243
PspXI vC'TCGA_Gb
Cuts at: 934 934
Size: 5282
PstI C_TGCA'G
Cuts at: 1178 2329 3014 1178
Size: 1151 685 3446
Fragments arranged by size:
3446 1151 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5282
PvuII CAG'CTG
Cuts at: 2190 2707 3067 2190
Size: 517 360 4405
Fragments arranged by size:
4405 517 360
RsrII CG'GwC_CG
Cuts at: 3477 3477
Size: 5282
SacI G_AGCT'C
Cuts at: 2296 2296
Size: 5282
SalI G'TCGA_C
Cuts at: 2319 2319
Size: 5282
SanDI GG'GwC_CC
Cuts at: 1258 1258
Size: 5282
SapI GCTCTTCn'nnn_
Cuts at: 3305 3515 3305
Size: 210 5072
SbfI CC_TGCA'GG
Cuts at: 2329 2329
Size: 5282
ScaI AGT'ACT
Cuts at: 4799 4799
Size: 5282
SfcI C'TryA_G
Cuts at: 33 1174 1971 2325 3010 4003 4194 33
Size: 1141 797 354 685 993 191 1121
Fragments arranged by size:
1141 1121 993 797 685 354 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 749 749
Size: 5282
SfoI GGC'GCC
Cuts at: 676 2714 2962 676
Size: 2038 248 2996
Fragments arranged by size:
2996 2038 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
SmaI CCC'GGG
Cuts at: 1370 2304 1370
Size: 934 4348
SmlI C'TyrA_G
Cuts at: 934 2124 2437 3352 3844 4106 934
Size: 1190 313 915 492 262 2110
Fragments arranged by size:
2110 1190 915 492 313 262
SnaBI TAC'GTA
Cuts at: 2274 2274
Size: 5282
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5282
SphI G_CATG'C
Cuts at: 2335 2835 2335
Size: 500 4782
StuI AGG'CCT
Cuts at: 611 662 1278 1577 2114 2122 611
Size: 51 616 299 537 8 3771
Fragments arranged by size:
3771 616 537 299 51 8
StyI C'CwwG_G
Cuts at: 2416 2770 2416
Size: 354 4928
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1090 1534 1798 1838 2865 2998 3457 3666 1090
Size: 444 264 40 1027 133 459 209 2706
Fragments arranged by size:
2706 1027 459 444 264 209 133 40
TatI w'GTAC_w
Cuts at: 518 551 2003 4797 518
Size: 33 1452 2794 1003
Fragments arranged by size:
2794 1452 1003 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 740 772 1073 1105 4799 4831 740
Size: 32 301 32 3694 32 1191
Fragments arranged by size:
3694 1191 301 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3079 3079
Size: 5282
XbaI T'CTAG_A
Cuts at: 2313 2313
Size: 5282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 280 819 2227 2777 280
Size: 539 1408 550 2785
Fragments arranged by size:
2785 1408 550 539
XhoI C'TCGA_G
Cuts at: 934 934
Size: 5282
XmaI C'CCGG_G
Cuts at: 1368 2302 1368
Size: 934 4348
XmnI GAAnn'nnTTC
Cuts at: 2177 2177
Size: 5282
ZraI GAC'GTC
Cuts at: 207 2247 207
Size: 2040 3242
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PsrI SacII
SexAI SgrAI SrfI SspI SwaI