Restriction map for
pF1KSDA1561
(Circular) (Six-base) MAPSORT of: pF1KSDA1561.seq Check: 8278 from: 1 to: 7385
pF1KSDA1561 7385 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 280 280
Size: 7385
AccI GT'mk_AC
Cuts at: 620 1362 4423 620
Size: 742 3061 3582
Fragments arranged by size:
3582 3061 742
Acc65I G'GTAC_C
Cuts at: 4401 4401
Size: 7385
AclI AA'CG_TT
Cuts at: 7225 7225
Size: 7385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 1528 1771 1778 3229 3550 3721 5210 5642
Size: 339 243 7 1451 321 171 1489 432
Cuts at: 5642 6389 6657 1189
Size: 747 268 1917
Fragments arranged by size:
1917 1489 1451 747 432 339 321 268
243 171 7
AflII C'TTAA_G
Cuts at: 1212 2499 2593 2614 1212
Size: 1287 94 21 5983
Fragments arranged by size:
5983 1287 94 21
AflIII A'CryG_T
Cuts at: 2747 3456 4737 5841 6818 2747
Size: 709 1281 1104 977 3314
Fragments arranged by size:
3314 1281 1104 977 709
AgeI A'CCGG_T
Cuts at: 5773 5773
Size: 7385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 7385
AlwNI CAG_nnn'CTG
Cuts at: 4690 6257 4690
Size: 1567 5818
ApaLI G'TGCA_C
Cuts at: 6155 6155
Size: 7385
ApoI r'AATT_y
Cuts at: 951 1061 1445 2017 2791 2812 3115 4389 6738
Size: 110 384 572 774 21 303 1274 2349
Cuts at: 6738 6994 951
Size: 256 1342
Fragments arranged by size:
2349 1342 1274 774 572 384 303 256
110 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 7385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7385
AvaI C'yCGr_G
Cuts at: 4405 4405
Size: 7385
BamHI G'GATC_C
Cuts at: 4410 4410
Size: 7385
BanI G'GyrC_C
Cuts at: 184 4401 4815 5063 5098 184
Size: 4217 414 248 35 2471
Fragments arranged by size:
4217 2471 414 248 35
BanII G_rGCy'C
Cuts at: 2038 2926 3047 4399 5429 2038
Size: 888 121 1352 1030 3994
Fragments arranged by size:
3994 1352 1030 888 121
BbeI G_GCGC'C
Cuts at: 188 4819 5067 188
Size: 4631 248 2506
Fragments arranged by size:
4631 2506 248
BbsI GAAGACnn'nnnn_
Cuts at: 330 889 2384 2639 330
Size: 559 1495 255 5076
Fragments arranged by size:
5076 1495 559 255
BciVI GTATCCnnnnn_n'
Cuts at: 5283 6044 7385 5283
Size: 761 1341 5283
Fragments arranged by size:
5283 1341 761
BclI T'GATC_A
Cuts at: 1260 4905 1260
Size: 3645 3740
BfrBI ATG'CAT
Cuts at: 466 1618 3065 466
Size: 1152 1447 4786
Fragments arranged by size:
4786 1447 1152
BglI GCCn_nnn'nGGC
Cuts at: 553 553
Size: 7385
BglII A'GATC_T
Cuts at: 331 1384 4900 6937 331
Size: 1053 3516 2037 779
Fragments arranged by size:
3516 2037 1053 779
BlpI GC'TnA_GC
Cuts at: 2162 4497 2162
Size: 2335 5050
Bme1580I G_kGCm'C
Cuts at: 167 5010 5103 6159 167
Size: 4843 93 1056 1393
Fragments arranged by size:
4843 1393 1056 93
BmgBI CAC'GTC
Cuts at: 162 2169 162
Size: 2007 5378
BmrI ACTGGGnnnn_n'
Cuts at: 1412 4597 4678 5013 7157 1412
Size: 3185 81 335 2144 1640
Fragments arranged by size:
3185 2144 1640 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 452 5752 452
Size: 5300 2085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4243 4324 4243
Size: 81 7304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 388 2158 2296 3705 4561 5440 5932 6230
Size: 267 1770 138 1409 856 879 492 298
Cuts at: 6230 121
Size: 1276
Fragments arranged by size:
1770 1409 1276 879 856 492 298 267
138
BsaI GGTCTCn'nnnn_
Cuts at: 7089 7089
Size: 7385
BsaAI yAC'GTr
Cuts at: 4377 4738 4377
Size: 361 7024
BsaBI GATnn'nnATC
Cuts at: 6 2804 4899 6936 6
Size: 2798 2095 2037 455
Fragments arranged by size:
2798 2095 2037 455
BsaHI Gr'CG_yC
Cuts at: 185 277 4816 5064 5766 7262 185
Size: 92 4539 248 702 1496 308
Fragments arranged by size:
4539 1496 702 308 248 92
BsaWI w'CCGG_w
Cuts at: 4574 5095 5773 6047 6194 6605 4574
Size: 521 678 274 147 411 5354
Fragments arranged by size:
5354 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 686 716 1496 1526 686
Size: 30 780 30 6545
Fragments arranged by size:
6545 780 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 165 707 2299 3115 165
Size: 542 1592 816 4435
Fragments arranged by size:
4435 1592 816 542
BseYI C'CCAG_C
Cuts at: 4810 5568 6145 6641 4810
Size: 758 577 496 5554
Fragments arranged by size:
5554 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 1651 2971 4283 518
Size: 1133 1320 1312 3620
Fragments arranged by size:
3620 1320 1312 1133
BsiEI CG_ry'CG
Cuts at: 104 4927 4973 6181 104
Size: 4823 46 1208 1308
Fragments arranged by size:
4823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2038 2909 2926 3047 4399 5177 5367 6159 2038
Size: 871 17 121 1352 778 190 792 3264
Fragments arranged by size:
3264 1352 871 792 778 190 121 17
BsmI GAATG_Cn'
Cuts at: 384 612 1549 3067 384
Size: 228 937 1518 4702
Fragments arranged by size:
4702 1518 937 228
Bsp1286I G_dGCh'C
Cuts at: 167 2038 2909 2926 3047 4399 5010 5103 5177
Size: 1871 871 17 121 1352 611 93 74
Cuts at: 5177 5367 5429 6159 167
Size: 190 62 730 1393
Fragments arranged by size:
1871 1393 1352 871 730 611 190 121
93 74 62 17
BspEI T'CCGG_A
Cuts at: 6605 6605
Size: 7385
BspHI T'CATG_A
Cuts at: 3288 6561 3288
Size: 3273 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 527 4435 4649 4951 5332 527
Size: 3908 214 302 381 2580
Fragments arranged by size:
3908 2580 381 302 214
BsrBI CCG'CTC
Cuts at: 50 697 5677 5731 7215 7380 50
Size: 647 4980 54 1484 165 55
Fragments arranged by size:
4980 1484 647 165 55 54
BsrDI GCAATG_nn'
Cuts at: 557 1475 5297 557
Size: 918 3822 2645
Fragments arranged by size:
3822 2645 918
BsrFI r'CCGG_y
Cuts at: 5383 5564 5773 5383
Size: 181 209 6995
Fragments arranged by size:
6995 209 181
BssHII G'CGCG_C
Cuts at: 200 200
Size: 7385
BssSI C'ACGA_G
Cuts at: 2908 5656 6014 2908
Size: 2748 358 4279
Fragments arranged by size:
4279 2748 358
BstAPI GCAn_nnn'nTGC
Cuts at: 162 4762 162
Size: 4600 2785
BstBI TT'CG_AA
Cuts at: 110 5746 110
Size: 5636 1749
BstYI r'GATC_y
Cuts at: 1 331 1384 4410 4877 4900 5235 5481 6493
Size: 330 1053 3026 467 23 335 246 1012
Cuts at: 6493 6579 6591 6937 1
Size: 86 12 346 449
Fragments arranged by size:
3026 1053 1012 467 449 346 335 330
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1363 1363
Size: 7385
Bsu36I CC'TnA_GG
Cuts at: 148 1722 148
Size: 1574 5811
BtgI C'CryG_G
Cuts at: 591 591
Size: 7385
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5335 5516 5679 5335
Size: 181 163 7041
Fragments arranged by size:
7041 181 163
BtsI GCAGTG_nn'
Cuts at: 419 3010 3437 4628 419
Size: 2591 427 1191 3176
Fragments arranged by size:
3176 2591 1191 427
DraI TTT'AAA
Cuts at: 1245 1451 2366 3120 4384 1245
Size: 206 915 754 1264 4246
Fragments arranged by size:
4246 1264 915 754 206
DraIII CAC_nnn'GTG
Cuts at: 1536 2110 4212 1536
Size: 574 2102 4709
Fragments arranged by size:
4709 2102 574
DrdI GACnn_nn'nnGTC
Cuts at: 5091 5949 5091
Size: 858 6527
EaeI y'GGCC_r
Cuts at: 822 2975 3866 4235 4970 5144 5535 5562 6907
Size: 2153 891 369 735 174 391 27 1345
Cuts at: 6907 822
Size: 1300
Fragments arranged by size:
2153 1345 1300 891 735 391 369 174
27
EagI C'GGCC_G
Cuts at: 4970 4970
Size: 7385
EarI CTCTTCn'nnn_
Cuts at: 124 443 1630 1844 2026 2717 2993 3526 3615
Size: 319 1187 214 182 691 276 533 89
Cuts at: 3615 5408 5618 124
Size: 1793 210 1891
Fragments arranged by size:
1891 1793 1187 691 533 319 276 214
210 182 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 642 5593 5903 6049 7195 642
Size: 4951 310 146 1146 832
Fragments arranged by size:
4951 1146 832 310 146
EcoICRI GAG'CTC
Cuts at: 2036 2924 3045 4397 2036
Size: 888 121 1352 5024
Fragments arranged by size:
5024 1352 888 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 1528 1771 1778 3229 3550 3721 4243 4324
Size: 339 243 7 1451 321 171 522 81
Cuts at: 4324 5210 5642 6389 6657 1189
Size: 886 432 747 268 1917
Fragments arranged by size:
1917 1451 886 747 522 432 339 321
268 243 171 81 7
EcoO109I rG'GnC_Cy
Cuts at: 596 4524 596
Size: 3928 3457
EcoRI G'AATT_C
Cuts at: 4389 4389
Size: 7385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1324 5404 1324
Size: 4080 3305
FspI TGC'GCA
Cuts at: 5166 5166
Size: 7385
HaeII r_GCGC'y
Cuts at: 188 4819 5067 6089 7068 188
Size: 4631 248 1022 979 505
Fragments arranged by size:
4631 1022 979 505 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 987 1019 1496 1528 2553 2585 2679 2711 3136
Size: 32 477 32 1025 32 94 32 425
Cuts at: 3136 3168 4182 4214 5227 5257 5259 5289 987
Size: 32 1014 32 1013 30 2 30 3083
Fragments arranged by size:
3083 1025 1014 1013 477 425 94 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 676 2249 3798 4424 676
Size: 1573 1549 626 3637
Fragments arranged by size:
3637 1573 1549 626
HindIII A'AGCT_T
Cuts at: 804 1209 1550 2155 2425 804
Size: 405 341 605 270 5764
Fragments arranged by size:
5764 605 405 341 270
HpaI GTT'AAC
Cuts at: 2249 2249
Size: 7385
KasI G'GCGC_C
Cuts at: 184 4815 5063 184
Size: 4631 248 2506
Fragments arranged by size:
4631 2506 248
KpnI G_GTAC'C
Cuts at: 4405 4405
Size: 7385
MfeI C'AATT_G
Cuts at: 495 2336 3086 3506 3586 495
Size: 1841 750 420 80 4294
Fragments arranged by size:
4294 1841 750 420 80
MluI A'CGCG_T
Cuts at: 6818 6818
Size: 7385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2444 3572 3645 3722 6056 6240 2444
Size: 1128 73 77 2334 184 3589
Fragments arranged by size:
3589 2334 1128 184 77 73
MscI TGG'CCA
Cuts at: 824 2977 3868 5146 824
Size: 2153 891 1278 3063
Fragments arranged by size:
3063 2153 1278 891
MslI CAynn'nnrTG
Cuts at: 1563 1957 4313 4351 5374 5501 1563
Size: 394 2356 38 1023 127 3447
Fragments arranged by size:
3447 2356 1023 394 127 38
MspA1I CmG'CkG
Cuts at: 579 1116 2011 2604 4188 4233 4496 4810 5170
Size: 537 895 593 1584 45 263 314 360
Cuts at: 5170 6183 6428 579
Size: 1013 245 1536
Fragments arranged by size:
1584 1536 1013 895 593 537 360 314
263 245 45
NaeI GCC'GGC
Cuts at: 5566 5566
Size: 7385
NarI GG'CG_CC
Cuts at: 185 4816 5064 185
Size: 4631 248 2506
Fragments arranged by size:
4631 2506 248
NcoI C'CATG_G
Cuts at: 591 591
Size: 7385
NdeI CA'TA_TG
Cuts at: 1373 1373
Size: 7385
NgoMIV G'CCGG_C
Cuts at: 5564 5564
Size: 7385
NsiI A_TGCA'T
Cuts at: 468 1620 3067 468
Size: 1152 1447 4786
Fragments arranged by size:
4786 1447 1152
NspI r_CATG'y
Cuts at: 208 466 514 966 3019 3460 3916 4438 4938
Size: 258 48 452 2053 441 456 522 500
Cuts at: 4938 5845 208
Size: 907 1748
Fragments arranged by size:
2053 1748 907 522 500 456 452 441
258 48
PacI TTA_AT'TAA
Cuts at: 785 785
Size: 7385
PciI A'CATG_T
Cuts at: 3456 5841 3456
Size: 2385 5000
PmeI GTTT'AAAC
Cuts at: 4384 4384
Size: 7385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6524 6556 6524
Size: 32 7353
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1064 1096 1397 1429 1064
Size: 32 301 32 7020
Fragments arranged by size:
7020 301 32 32
PstI C_TGCA'G
Cuts at: 426 524 4205 4432 5117 426
Size: 98 3681 227 685 2694
Fragments arranged by size:
3681 2694 685 227 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7385
PvuII CAG'CTG
Cuts at: 579 1116 2011 2604 4188 4233 4810 5170 579
Size: 537 895 593 1584 45 577 360 2794
Fragments arranged by size:
2794 1584 895 593 577 537 360 45
RsrII CG'GwC_CG
Cuts at: 5580 5580
Size: 7385
SacI G_AGCT'C
Cuts at: 2038 2926 3047 4399 2038
Size: 888 121 1352 5024
Fragments arranged by size:
5024 1352 888 121
SalI G'TCGA_C
Cuts at: 4422 4422
Size: 7385
SapI GCTCTTCn'nnn_
Cuts at: 124 443 2026 5408 5618 124
Size: 319 1583 3382 210 1891
Fragments arranged by size:
3382 1891 1583 319 210
SbfI CC_TGCA'GG
Cuts at: 524 4432 524
Size: 3908 3477
ScaI AGT'ACT
Cuts at: 6902 6902
Size: 7385
SfcI C'TryA_G
Cuts at: 33 422 487 520 2982 4201 4428 5113 6106
Size: 389 65 33 2462 1219 227 685 993
Cuts at: 6106 6297 33
Size: 191 1121
Fragments arranged by size:
2462 1219 1121 993 685 389 227 191
65 33
SfoI GGC'GCC
Cuts at: 186 4817 5065 186
Size: 4631 248 2506
Fragments arranged by size:
4631 2506 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7385
SmaI CCC'GGG
Cuts at: 4407 4407
Size: 7385
SmlI C'TyrA_G
Cuts at: 136 367 1212 2137 2311 2499 2593 2614 3684
Size: 231 845 925 174 188 94 21 1070
Cuts at: 3684 4540 5455 5947 6209 136
Size: 856 915 492 262 1312
Fragments arranged by size:
1312 1070 925 915 856 845 492 262
231 188 174 94 21
SnaBI TAC'GTA
Cuts at: 4377 4377
Size: 7385
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7385
SphI G_CATG'C
Cuts at: 208 514 3019 3916 4438 4938 208
Size: 306 2505 897 522 500 2655
Fragments arranged by size:
2655 2505 897 522 500 306
SspI AAT'ATT
Cuts at: 1011 1249 2583 2843 1011
Size: 238 1334 260 5553
Fragments arranged by size:
5553 1334 260 238
StyI C'CwwG_G
Cuts at: 591 4519 4873 591
Size: 3928 354 3103
Fragments arranged by size:
3928 3103 354
SwaI ATTT'AAAT
Cuts at: 1245 1245
Size: 7385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 248 1517 4968 5101 5560 5769 248
Size: 1269 3451 133 459 209 1864
Fragments arranged by size:
3451 1864 1269 459 209 133
TatI w'GTAC_w
Cuts at: 491 1348 2888 6900 491
Size: 857 1540 4012 976
Fragments arranged by size:
4012 1540 976 857
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6902 6934 6902
Size: 32 7353
Tth111I GACn'n_nGTC
Cuts at: 5182 5182
Size: 7385
XbaI T'CTAG_A
Cuts at: 4416 4416
Size: 7385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4880 4880
Size: 7385
XmaI C'CCGG_G
Cuts at: 4405 4405
Size: 7385
XmnI GAAnn'nnTTC
Cuts at: 4322 4322
Size: 7385
ZraI GAC'GTC
Cuts at: 278 278
Size: 7385
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PacI PciI
PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BmtI Bpu10I BsiWI BsmBI BsrGI
BstEII BstXI ClaI CspCI EcoNI EcoRV FseI FspAI
NheI NotI NruI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SrfI StuI XhoI