Restriction map for
pF1KSDA1562
(Circular) (Six-base) MAPSORT of: pF1KSDA1562.seq Check: 5125 from: 1 to: 6542
pF1KSDA1562 6542 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 3580 3580
Size: 6542
Acc65I G'GTAC_C
Cuts at: 3558 3558
Size: 6542
AclI AA'CG_TT
Cuts at: 1841 2048 6382 1841
Size: 207 4334 2001
Fragments arranged by size:
4334 2001 207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 769 963 1330 2161 2177 4367 4799 5546
Size: 18 194 367 831 16 2190 432 747
Cuts at: 5546 5814 751
Size: 268 1479
Fragments arranged by size:
2190 1479 831 747 432 367 268 194
18 16
AflIII A'CryG_T
Cuts at: 2963 3894 4998 5975 2963
Size: 931 1104 977 3530
Fragments arranged by size:
3530 1104 977 931
AgeI A'CCGG_T
Cuts at: 4930 4930
Size: 6542
AhdI GACnn_n'nnGTC
Cuts at: 1454 1454
Size: 6542
AleI CACnn'nnGTG
Cuts at: 1753 1810 2407 3356 1753
Size: 57 597 949 4939
Fragments arranged by size:
4939 949 597 57
AlwNI CAG_nnn'CTG
Cuts at: 1600 3847 5414 1600
Size: 2247 1567 2728
Fragments arranged by size:
2728 2247 1567
ApaLI G'TGCA_C
Cuts at: 5312 5312
Size: 6542
ApoI r'AATT_y
Cuts at: 210 342 630 1119 1537 2571 2725 2855 2983
Size: 132 288 489 418 1034 154 130 128
Cuts at: 2983 3546 5895 6151 210
Size: 563 2349 256 601
Fragments arranged by size:
2349 1034 601 563 489 418 288 256
154 132 130 128
AseI AT'TA_AT
Cuts at: 20 515 20
Size: 495 6047
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6542
AvaI C'yCGr_G
Cuts at: 3562 3562
Size: 6542
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1244 1277 1244
Size: 33 6509
BamHI G'GATC_C
Cuts at: 939 1242 3567 939
Size: 303 2325 3914
Fragments arranged by size:
3914 2325 303
BanI G'GyrC_C
Cuts at: 2409 3558 3972 4220 4255 2409
Size: 1149 414 248 35 4696
Fragments arranged by size:
4696 1149 414 248 35
BanII G_rGCy'C
Cuts at: 3556 4586 3556
Size: 1030 5512
BbeI G_GCGC'C
Cuts at: 3976 4224 3976
Size: 248 6294
BbsI GAAGACnn'nnnn_
Cuts at: 1357 2936 1357
Size: 1579 4963
BbvCI CC'TCA_GC
Cuts at: 1436 2117 1436
Size: 681 5861
BciVI GTATCCnnnnn_n'
Cuts at: 3111 4440 5201 6542 3111
Size: 1329 761 1341 3111
Fragments arranged by size:
3111 1341 1329 761
BclI T'GATC_A
Cuts at: 455 1725 4062 455
Size: 1270 2337 2935
Fragments arranged by size:
2935 2337 1270
BglI GCCn_nnn'nGGC
Cuts at: 2379 2379
Size: 6542
BglII A'GATC_T
Cuts at: 541 924 1408 2440 4057 6094 541
Size: 383 484 1032 1617 2037 989
Fragments arranged by size:
2037 1617 1032 989 484 383
BlpI GC'TnA_GC
Cuts at: 3038 3654 3038
Size: 616 5926
Bme1580I G_kGCm'C
Cuts at: 4167 4260 5316 4167
Size: 93 1056 5393
Fragments arranged by size:
5393 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 211 525 1448 3122 3754 3835 4170 6314 211
Size: 314 923 1674 632 81 335 2144 439
Fragments arranged by size:
2144 1674 923 632 439 335 314 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2592 2634 4909 2592
Size: 42 2275 4225
Fragments arranged by size:
4225 2275 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3250 3339 3250
Size: 89 6453
Bpu10I CC'TnA_GC
Cuts at: 1436 2117 1436
Size: 681 5861
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1088 3046 3718 4597 5089 5387 1088
Size: 1958 672 879 492 298 2243
Fragments arranged by size:
2243 1958 879 672 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2622 6246 2622
Size: 3624 2918
BsaAI yAC'GTr
Cuts at: 2562 3534 3895 2562
Size: 972 361 5209
Fragments arranged by size:
5209 972 361
BsaBI GATnn'nnATC
Cuts at: 6 1601 4056 6093 6
Size: 1595 2455 2037 455
Fragments arranged by size:
2455 2037 1595 455
BsaHI Gr'CG_yC
Cuts at: 3973 4221 4923 6419 3973
Size: 248 702 1496 4096
Fragments arranged by size:
4096 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3052 3731 4252 4930 5204 5351 5762 3052
Size: 679 521 678 274 147 411 3832
Fragments arranged by size:
3832 679 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 736 766 1295 1325 736
Size: 30 529 30 5953
Fragments arranged by size:
5953 529 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 338 2014 2899 3409 338
Size: 1676 885 510 3471
Fragments arranged by size:
3471 1676 885 510
BseYI C'CCAG_C
Cuts at: 1128 2999 3967 4725 5302 5798 1128
Size: 1871 968 758 577 496 1872
Fragments arranged by size:
1872 1871 968 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 2276 2862 2910 3382 593
Size: 1683 586 48 472 3753
Fragments arranged by size:
3753 1683 586 472 48
BsiEI CG_ry'CG
Cuts at: 104 4084 4130 5338 104
Size: 3980 46 1208 1308
Fragments arranged by size:
3980 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1817 3440 3556 4334 4524 5316 1817
Size: 1623 116 778 190 792 3043
Fragments arranged by size:
3043 1623 792 778 190 116
BsmI GAATG_Cn'
Cuts at: 144 590 936 1968 3205 144
Size: 446 346 1032 1237 3481
Fragments arranged by size:
3481 1237 1032 446 346
BsmBI CGTCTCn'nnnn_
Cuts at: 2462 3172 2462
Size: 710 5832
Bsp1286I G_dGCh'C
Cuts at: 1817 3440 3556 4167 4260 4334 4524 4586 5316
Size: 1623 116 611 93 74 190 62 730
Cuts at: 5316 1817
Size: 3043
Fragments arranged by size:
3043 1623 730 611 190 116 93 74
62
BspEI T'CCGG_A
Cuts at: 5762 5762
Size: 6542
BspHI T'CATG_A
Cuts at: 1728 5718 1728
Size: 3990 2552
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1981 2604 3592 3806 4108 4489 1981
Size: 623 988 214 302 381 4034
Fragments arranged by size:
4034 988 623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4834 4888 6372 6537 50
Size: 4784 54 1484 165 55
Fragments arranged by size:
4784 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1134 1864 2192 4454 1134
Size: 730 328 2262 3222
Fragments arranged by size:
3222 2262 730 328
BsrFI r'CCGG_y
Cuts at: 2501 4540 4721 4930 2501
Size: 2039 181 209 4113
Fragments arranged by size:
4113 2039 209 181
BssSI C'ACGA_G
Cuts at: 4813 5171 4813
Size: 358 6184
BstAPI GCAn_nnn'nTGC
Cuts at: 2254 3919 2254
Size: 1665 4877
BstBI TT'CG_AA
Cuts at: 110 4903 110
Size: 4793 1749
BstEII G'GTnAC_C
Cuts at: 3268 3268
Size: 6542
BstXI CCAn_nnnn'nTGG
Cuts at: 1005 3228 3264 3486 1005
Size: 2223 36 222 4061
Fragments arranged by size:
4061 2223 222 36
BstYI r'GATC_y
Cuts at: 1 541 924 939 1242 1408 1596 2320 2440
Size: 540 383 15 303 166 188 724 120
Cuts at: 2440 3398 3567 4034 4057 4392 4638 5650 5736
Size: 958 169 467 23 335 246 1012 86
Cuts at: 5736 5748 6094 1
Size: 12 346 449
Fragments arranged by size:
1012 958 724 540 467 449 383 346
335 303 246 188 169 166 120 86
23 15 12
Bsu36I CC'TnA_GG
Cuts at: 2241 2241
Size: 6542
BtgI C'CryG_G
Cuts at: 935 2748 3389 935
Size: 1813 641 4088
Fragments arranged by size:
4088 1813 641
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4492 4673 4836 4492
Size: 181 163 6198
Fragments arranged by size:
6198 181 163
BtsI GCAGTG_nn'
Cuts at: 773 2264 2929 3159 3210 3785 773
Size: 1491 665 230 51 575 3530
Fragments arranged by size:
3530 1491 665 575 230 51
DraI TTT'AAA
Cuts at: 1017 1341 3541 1017
Size: 324 2200 4018
Fragments arranged by size:
4018 2200 324
DrdI GACnn_nn'nnGTC
Cuts at: 4248 5106 4248
Size: 858 5684
EaeI y'GGCC_r
Cuts at: 2339 2745 4127 4301 4692 4719 6064 2339
Size: 406 1382 174 391 27 1345 2817
Fragments arranged by size:
2817 1382 1345 406 391 174 27
EagI C'GGCC_G
Cuts at: 4127 4127
Size: 6542
EarI CTCTTCn'nnn_
Cuts at: 3061 4565 4775 3061
Size: 1504 210 4828
Fragments arranged by size:
4828 1504 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3511 4750 5060 5206 6352 3511
Size: 1239 310 146 1146 3701
Fragments arranged by size:
3701 1239 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3554 3554
Size: 6542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 769 963 1330 2161 2177 3250 3339 4367
Size: 18 194 367 831 16 1073 89 1028
Cuts at: 4367 4799 5546 5814 751
Size: 432 747 268 1479
Fragments arranged by size:
1479 1073 1028 831 747 432 367 268
194 89 18 16
EcoO109I rG'GnC_Cy
Cuts at: 1858 2895 3244 3681 1858
Size: 1037 349 437 4719
Fragments arranged by size:
4719 1037 437 349
EcoRI G'AATT_C
Cuts at: 2855 2983 3546 2855
Size: 128 563 5851
Fragments arranged by size:
5851 563 128
EcoRV GAT'ATC
Cuts at: 563 1497 563
Size: 934 5608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3264 4561 3264
Size: 1297 5245
FspI TGC'GCA
Cuts at: 4323 4323
Size: 6542
HaeII r_GCGC'y
Cuts at: 956 3976 4224 5246 6225 956
Size: 3020 248 1022 979 1273
Fragments arranged by size:
3020 1273 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 736 768 856 888 1440 1472 1936 1968 2011
Size: 32 88 32 552 32 464 32 43
Cuts at: 2011 2043 2305 2337 4384 4414 4416 4446 736
Size: 32 262 32 2047 30 2 30 2832
Fragments arranged by size:
2832 2047 552 464 262 88 43 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3581 3581
Size: 6542
HindIII A'AGCT_T
Cuts at: 295 1318 295
Size: 1023 5519
KasI G'GCGC_C
Cuts at: 3972 4220 3972
Size: 248 6294
KpnI G_GTAC'C
Cuts at: 3562 3562
Size: 6542
MfeI C'AATT_G
Cuts at: 398 1940 398
Size: 1542 5000
MluI A'CGCG_T
Cuts at: 5975 5975
Size: 6542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 225 3245 5213 5397 225
Size: 3020 1968 184 1370
Fragments arranged by size:
3020 1968 1370 184
MscI TGG'CCA
Cuts at: 4303 4303
Size: 6542
MslI CAynn'nnrTG
Cuts at: 1328 1753 1810 2028 2407 3356 3449 4531 4658
Size: 425 57 218 379 949 93 1082 127
Cuts at: 4658 1328
Size: 3212
Fragments arranged by size:
3212 1082 949 425 379 218 127 93
57
MspA1I CmG'CkG
Cuts at: 1976 3653 3967 4327 5340 5585 1976
Size: 1677 314 360 1013 245 2933
Fragments arranged by size:
2933 1677 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2503 4723 2503
Size: 2220 4322
NarI GG'CG_CC
Cuts at: 3973 4221 3973
Size: 248 6294
NcoI C'CATG_G
Cuts at: 3389 3389
Size: 6542
NdeI CA'TA_TG
Cuts at: 1356 1674 1356
Size: 318 6224
NgoMIV G'CCGG_C
Cuts at: 2501 4721 2501
Size: 2220 4322
NspI r_CATG'y
Cuts at: 2967 3595 4095 5002 2967
Size: 628 500 907 4507
Fragments arranged by size:
4507 907 628 500
PciI A'CATG_T
Cuts at: 2963 4998 2963
Size: 2035 4507
PflMI CCAn_nnn'nTGG
Cuts at: 622 1696 622
Size: 1074 5468
PfoI T'CCnGG_A
Cuts at: 2689 2689
Size: 6542
PmeI GTTT'AAAC
Cuts at: 3541 3541
Size: 6542
PmlI CAC'GTG
Cuts at: 2562 2562
Size: 6542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5681 5713 5681
Size: 32 6510
PpuMI rG'GwC_Cy
Cuts at: 2895 3244 2895
Size: 349 6193
PsiI TTA'TAA
Cuts at: 1147 1147
Size: 6542
PstI C_TGCA'G
Cuts at: 482 2335 3048 3589 4274 482
Size: 1853 713 541 685 2750
Fragments arranged by size:
2750 1853 713 685 541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6542
PvuII CAG'CTG
Cuts at: 1976 3967 4327 1976
Size: 1991 360 4191
Fragments arranged by size:
4191 1991 360
RsrII CG'GwC_CG
Cuts at: 4737 4737
Size: 6542
SacI G_AGCT'C
Cuts at: 3556 3556
Size: 6542
SalI G'TCGA_C
Cuts at: 3579 3579
Size: 6542
SanDI GG'GwC_CC
Cuts at: 3244 3244
Size: 6542
SapI GCTCTTCn'nnn_
Cuts at: 4565 4775 4565
Size: 210 6332
SbfI CC_TGCA'GG
Cuts at: 3589 3589
Size: 6542
ScaI AGT'ACT
Cuts at: 6059 6059
Size: 6542
SfcI C'TryA_G
Cuts at: 33 478 1463 2331 2697 3044 3585 4270 5263
Size: 445 985 868 366 347 541 685 993
Cuts at: 5263 5454 33
Size: 191 1121
Fragments arranged by size:
1121 993 985 868 685 541 445 366
347 191
SfoI GGC'GCC
Cuts at: 3974 4222 3974
Size: 248 6294
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6542
SmaI CCC'GGG
Cuts at: 3564 3564
Size: 6542
SmlI C'TyrA_G
Cuts at: 1103 3025 3697 4612 5104 5366 1103
Size: 1922 672 915 492 262 2279
Fragments arranged by size:
2279 1922 915 672 492 262
SnaBI TAC'GTA
Cuts at: 3534 3534
Size: 6542
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6542
SphI G_CATG'C
Cuts at: 3595 4095 3595
Size: 500 6042
SspI AAT'ATT
Cuts at: 2686 2686
Size: 6542
StyI C'CwwG_G
Cuts at: 2210 3389 3676 4030 2210
Size: 1179 287 354 4722
Fragments arranged by size:
4722 1179 354 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1467 2378 2605 2806 3206 4125 4258 4717 4926
Size: 911 227 201 400 919 133 459 209
Cuts at: 4926 1467
Size: 3083
Fragments arranged by size:
3083 919 911 459 400 227 209 201
133
TatI w'GTAC_w
Cuts at: 198 2179 6057 198
Size: 1981 3878 683
Fragments arranged by size:
3878 1981 683
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1502 1534 6059 6091 1502
Size: 32 4525 32 1953
Fragments arranged by size:
4525 1953 32 32
Tth111I GACn'n_nGTC
Cuts at: 1331 4339 1331
Size: 3008 3534
XbaI T'CTAG_A
Cuts at: 3573 3573
Size: 6542
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2052 3451 3486 4037 2052
Size: 1399 35 551 4557
Fragments arranged by size:
4557 1399 551 35
XmaI C'CCGG_G
Cuts at: 3562 3562
Size: 6542
XmnI GAAnn'nnTTC
Cuts at: 2008 2008
Size: 6542
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BcgI BfrBI BmgBI BmtI BsiWI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
NheI NotI NruI NsiI PacI PasI PshAI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI