Restriction map for
pF1KSDA1572
(Circular) (Six-base) MAPSORT of: pF1KSDA1572.seq Check: 855 from: 1 to: 4460
pF1KSDA1572 4460 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 920 1026 1498 920
Size: 106 472 3882
Fragments arranged by size:
3882 472 106
Acc65I G'GTAC_C
Cuts at: 1476 1476
Size: 4460
AclI AA'CG_TT
Cuts at: 4300 4300
Size: 4460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 653 2285 2717 3464 3732 439
Size: 214 1632 432 747 268 1167
Fragments arranged by size:
1632 1167 747 432 268 214
AflII C'TTAA_G
Cuts at: 297 1422 297
Size: 1125 3335
AflIII A'CryG_T
Cuts at: 1812 2916 3893 1812
Size: 1104 977 2379
Fragments arranged by size:
2379 1104 977
AgeI A'CCGG_T
Cuts at: 2848 2848
Size: 4460
AleI CACnn'nnGTG
Cuts at: 1076 1076
Size: 4460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 602 602
Size: 4460
AlwNI CAG_nnn'CTG
Cuts at: 492 1386 1765 3332 492
Size: 894 379 1567 1620
Fragments arranged by size:
1620 1567 894 379
ApaLI G'TGCA_C
Cuts at: 480 3230 480
Size: 2750 1710
ApoI r'AATT_y
Cuts at: 217 406 789 1464 3813 4069 217
Size: 189 383 675 2349 256 608
Fragments arranged by size:
2349 675 608 383 256 189
AseI AT'TA_AT
Cuts at: 20 20
Size: 4460
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
AvaI C'yCGr_G
Cuts at: 1480 1480
Size: 4460
BamHI G'GATC_C
Cuts at: 1485 1485
Size: 4460
BanI G'GyrC_C
Cuts at: 1476 1890 2138 2173 1476
Size: 414 248 35 3763
Fragments arranged by size:
3763 414 248 35
BanII G_rGCy'C
Cuts at: 773 1474 2504 773
Size: 701 1030 2729
Fragments arranged by size:
2729 1030 701
BbeI G_GCGC'C
Cuts at: 1894 2142 1894
Size: 248 4212
BbsI GAAGACnn'nnnn_
Cuts at: 641 641
Size: 4460
BciVI GTATCCnnnnn_n'
Cuts at: 2358 3119 4460 2358
Size: 761 1341 2358
Fragments arranged by size:
2358 1341 761
BclI T'GATC_A
Cuts at: 1980 1980
Size: 4460
BglII A'GATC_T
Cuts at: 141 1975 4012 141
Size: 1834 2037 589
Fragments arranged by size:
2037 1834 589
BlpI GC'TnA_GC
Cuts at: 1572 1572
Size: 4460
Bme1580I G_kGCm'C
Cuts at: 484 952 2085 2178 3234 484
Size: 468 1133 93 1056 1710
Fragments arranged by size:
1710 1133 1056 468 93
BmrI ACTGGGnnnn_n'
Cuts at: 558 1672 1753 2088 4232 558
Size: 1114 81 335 2144 786
Fragments arranged by size:
2144 1114 786 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 575 2827 575
Size: 2252 2208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1000
Size: 4460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1636 2515 3007 3305 1636
Size: 879 492 298 2791
Fragments arranged by size:
2791 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 296 4164 296
Size: 3868 592
BsaAI yAC'GTr
Cuts at: 1452 1813 1452
Size: 361 4099
BsaBI GATnn'nnATC
Cuts at: 6 1974 4011 6
Size: 1968 2037 455
Fragments arranged by size:
2037 1968 455
BsaHI Gr'CG_yC
Cuts at: 1891 2139 2841 4337 1891
Size: 248 702 1496 2014
Fragments arranged by size:
2014 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1649 2170 2848 3122 3269 3680 1649
Size: 521 678 274 147 411 2429
Fragments arranged by size:
2429 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 773 1885 2643 3220 3716 773
Size: 1112 758 577 496 1517
Fragments arranged by size:
1517 1112 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2002 2048 3256 104
Size: 1898 46 1208 1308
Fragments arranged by size:
1898 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 484 1474 2252 2442 3234 484
Size: 990 778 190 792 1710
Fragments arranged by size:
1710 990 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 484 773 952 1474 2085 2178 2252 2442 2504
Size: 289 179 522 611 93 74 190 62
Cuts at: 2504 3234 484
Size: 730 1710
Fragments arranged by size:
1710 730 611 522 289 190 179 93
74 62
BspEI T'CCGG_A
Cuts at: 3680 3680
Size: 4460
BspHI T'CATG_A
Cuts at: 3636 3636
Size: 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 274 1384 1510 1724 2026 2407 274
Size: 1110 126 214 302 381 2327
Fragments arranged by size:
2327 1110 381 302 214 126
BsrBI CCG'CTC
Cuts at: 50 2752 2806 4290 4455 50
Size: 2702 54 1484 165 55
Fragments arranged by size:
2702 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2372 2372
Size: 4460
BsrFI r'CCGG_y
Cuts at: 2458 2639 2848 2458
Size: 181 209 4070
Fragments arranged by size:
4070 209 181
BsrGI T'GTAC_A
Cuts at: 1293 1330 1293
Size: 37 4423
BssSI C'ACGA_G
Cuts at: 2731 3089 2731
Size: 358 4102
BstAPI GCAn_nnn'nTGC
Cuts at: 1837 1837
Size: 4460
BstBI TT'CG_AA
Cuts at: 110 2821 110
Size: 2711 1749
BstYI r'GATC_y
Cuts at: 1 141 1485 1952 1975 2310 2556 3568 3654
Size: 140 1344 467 23 335 246 1012 86
Cuts at: 3654 3666 4012 1
Size: 12 346 449
Fragments arranged by size:
1344 1012 467 449 346 335 246 140
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2410 2591 2754 2410
Size: 181 163 4116
Fragments arranged by size:
4116 181 163
BtsI GCAGTG_nn'
Cuts at: 749 870 1703 749
Size: 121 833 3506
Fragments arranged by size:
3506 833 121
DraI TTT'AAA
Cuts at: 1001 1459 1001
Size: 458 4002
DraIII CAC_nnn'GTG
Cuts at: 553 553
Size: 4460
DrdI GACnn_nn'nnGTC
Cuts at: 2166 3024 2166
Size: 858 3602
EaeI y'GGCC_r
Cuts at: 2045 2219 2610 2637 3982 2045
Size: 174 391 27 1345 2523
Fragments arranged by size:
2523 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2045 2045
Size: 4460
EarI CTCTTCn'nnn_
Cuts at: 203 2483 2693 203
Size: 2280 210 1970
Fragments arranged by size:
2280 1970 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2668 2978 3124 4270 2668
Size: 310 146 1146 2858
Fragments arranged by size:
2858 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1472 1472
Size: 4460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 653 1000 2285 2717 3464 3732 439
Size: 214 347 1285 432 747 268 1167
Fragments arranged by size:
1285 1167 747 432 347 268 214
EcoO109I rG'GnC_Cy
Cuts at: 489 1383 1599 489
Size: 894 216 3350
Fragments arranged by size:
3350 894 216
EcoRI G'AATT_C
Cuts at: 406 1464 406
Size: 1058 3402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1078 1161 2479 1078
Size: 83 1318 3059
Fragments arranged by size:
3059 1318 83
FspI TGC'GCA
Cuts at: 2241 2241
Size: 4460
HaeII r_GCGC'y
Cuts at: 1894 2142 3164 4143 1894
Size: 248 1022 979 2211
Fragments arranged by size:
2211 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 362 394 1032 1064 2302 2332 2334
Size: 32 202 32 638 32 1238 30 2
Cuts at: 2334 2364 128
Size: 30 2224
Fragments arranged by size:
2224 1238 638 202 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1499 1499
Size: 4460
KasI G'GCGC_C
Cuts at: 1890 2138 1890
Size: 248 4212
KpnI G_GTAC'C
Cuts at: 1480 1480
Size: 4460
MfeI C'AATT_G
Cuts at: 373 373
Size: 4460
MluI A'CGCG_T
Cuts at: 3893 3893
Size: 4460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3131 3315 3131
Size: 184 4276
MscI TGG'CCA
Cuts at: 2221 2221
Size: 4460
MslI CAynn'nnrTG
Cuts at: 842 877 1076 1078 2449 2576 842
Size: 35 199 2 1371 127 2726
Fragments arranged by size:
2726 1371 199 127 35 2
MspA1I CmG'CkG
Cuts at: 1251 1571 1885 2245 3258 3503 1251
Size: 320 314 360 1013 245 2208
Fragments arranged by size:
2208 1013 360 320 314 245
NaeI GCC'GGC
Cuts at: 2641 2641
Size: 4460
NarI GG'CG_CC
Cuts at: 1891 2139 1891
Size: 248 4212
NgoMIV G'CCGG_C
Cuts at: 2639 2639
Size: 4460
NspI r_CATG'y
Cuts at: 1077 1087 1331 1513 2013 2920 1077
Size: 10 244 182 500 907 2617
Fragments arranged by size:
2617 907 500 244 182 10
PciI A'CATG_T
Cuts at: 2916 2916
Size: 4460
PfoI T'CCnGG_A
Cuts at: 1385 1385
Size: 4460
PmeI GTTT'AAAC
Cuts at: 1459 1459
Size: 4460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4428
PpuMI rG'GwC_Cy
Cuts at: 1383 1383
Size: 4460
PsiI TTA'TAA
Cuts at: 505 505
Size: 4460
PstI C_TGCA'G
Cuts at: 285 1507 2192 285
Size: 1222 685 2553
Fragments arranged by size:
2553 1222 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4460
PvuII CAG'CTG
Cuts at: 1251 1885 2245 1251
Size: 634 360 3466
Fragments arranged by size:
3466 634 360
RsrII CG'GwC_CG
Cuts at: 2655 2655
Size: 4460
SacI G_AGCT'C
Cuts at: 1474 1474
Size: 4460
SalI G'TCGA_C
Cuts at: 1497 1497
Size: 4460
SapI GCTCTTCn'nnn_
Cuts at: 2483 2693 2483
Size: 210 4250
SbfI CC_TGCA'GG
Cuts at: 1507 1507
Size: 4460
ScaI AGT'ACT
Cuts at: 1437 3977 1437
Size: 2540 1920
SfcI C'TryA_G
Cuts at: 33 281 931 1503 2188 3181 3372 33
Size: 248 650 572 685 993 191 1121
Fragments arranged by size:
1121 993 685 650 572 248 191
SfoI GGC'GCC
Cuts at: 1892 2140 1892
Size: 248 4212
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
SmaI CCC'GGG
Cuts at: 1482 1482
Size: 4460
SmlI C'TyrA_G
Cuts at: 297 1422 1615 2530 3022 3284 297
Size: 1125 193 915 492 262 1473
Fragments arranged by size:
1473 1125 915 492 262 193
SnaBI TAC'GTA
Cuts at: 1452 1452
Size: 4460
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4460
SphI G_CATG'C
Cuts at: 1087 1513 2013 1087
Size: 426 500 3534
Fragments arranged by size:
3534 500 426
SspI AAT'ATT
Cuts at: 909 909
Size: 4460
StuI AGG'CCT
Cuts at: 929 929
Size: 4460
StyI C'CwwG_G
Cuts at: 1594 1948 1594
Size: 354 4106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2043 2176 2635 2844 2043
Size: 133 459 209 3659
Fragments arranged by size:
3659 459 209 133
TatI w'GTAC_w
Cuts at: 1293 1330 1435 3975 1293
Size: 37 105 2540 1778
Fragments arranged by size:
2540 1778 105 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 4428
Tth111I GACn'n_nGTC
Cuts at: 2257 2257
Size: 4460
XbaI T'CTAG_A
Cuts at: 1491 1491
Size: 4460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1955 1955
Size: 4460
XmaI C'CCGG_G
Cuts at: 1480 1480
Size: 4460
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SwaI XhoI XmnI ZraI