Restriction map for
pF1KSDA1576
(Circular) (Six-base) MAPSORT of: pF1KSDA1576.seq Check: 9224 from: 1 to: 4394
pF1KSDA1576 4394 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1005 1005
Size: 4394
AccI GT'mk_AC
Cuts at: 563 1432 563
Size: 869 3525
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 878 975 2219 2651 3398 3666 285
Size: 593 97 1244 432 747 268 1013
Fragments arranged by size:
1244 1013 747 593 432 268 97
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AleI CACnn'nnGTG
Cuts at: 550 550
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 1699 3266 1699
Size: 1567 2827
ApaLI G'TGCA_C
Cuts at: 682 3164 682
Size: 2482 1912
ApoI r'AATT_y
Cuts at: 1398 3747 4003 1398
Size: 2349 256 1789
Fragments arranged by size:
2349 1789 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 232 316 1414 232
Size: 84 1098 3212
Fragments arranged by size:
3212 1098 84
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 426 1410 1824 2072 2107 426
Size: 984 414 248 35 2713
Fragments arranged by size:
2713 984 414 248 35
BanII G_rGCy'C
Cuts at: 284 338 1148 1194 1408 2438 284
Size: 54 810 46 214 1030 2240
Fragments arranged by size:
2240 1030 810 214 54 46
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 910 910
Size: 4394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 583 617 583
Size: 34 4360
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglI GCCn_nnn'nGGC
Cuts at: 1109 1109
Size: 4394
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 719 1506 719
Size: 787 3607
Bme1580I G_kGCm'C
Cuts at: 686 2019 2112 3168 686
Size: 1333 93 1056 1912
Fragments arranged by size:
1912 1333 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1606 1687 2022 4166 1606
Size: 81 335 2144 1834
Fragments arranged by size:
2144 1834 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 1216 992
Size: 224 4170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1570 2449 2941 3239 322
Size: 1248 879 492 298 1477
Fragments arranged by size:
1477 1248 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 488 916 970 1300 4098 488
Size: 428 54 330 2798 784
Fragments arranged by size:
2798 784 428 330 54
BsaAI yAC'GTr
Cuts at: 831 1386 1747 831
Size: 555 361 3478
Fragments arranged by size:
3478 555 361
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 239 1825 2073 2775 4271 239
Size: 1586 248 702 1496 362
Fragments arranged by size:
1586 1496 702 362 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3056 3203 3614 1583
Size: 521 678 274 147 411 2363
Fragments arranged by size:
2363 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 485 515 1263 1293 485
Size: 30 748 30 3586
Fragments arranged by size:
3586 748 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 1063 1213 1342 1345 169
Size: 894 150 129 3 3218
Fragments arranged by size:
3218 894 150 129 3
BseYI C'CCAG_C
Cuts at: 1819 2577 3154 3650 1819
Size: 758 577 496 2563
Fragments arranged by size:
2563 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 528
Size: 4394
BsiEI CG_ry'CG
Cuts at: 104 1233 1936 1982 3190 104
Size: 1129 703 46 1208 1308
Fragments arranged by size:
1308 1208 1129 703 46
BsiHKAI G_wGCw'C
Cuts at: 338 680 686 1408 2186 2376 3168 338
Size: 342 6 722 778 190 792 1564
Fragments arranged by size:
1564 792 778 722 342 190 6
BsmI GAATG_Cn'
Cuts at: 604 604
Size: 4394
BsmBI CGTCTCn'nnnn_
Cuts at: 151 151
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 284 338 680 686 1148 1194 1408 2019 2112
Size: 54 342 6 462 46 214 611 93
Cuts at: 2112 2186 2376 2438 3168 284
Size: 74 190 62 730 1510
Fragments arranged by size:
1510 730 611 462 342 214 190 93
74 62 54 46 6
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1005 1444 1658 1960 2341 1005
Size: 439 214 302 381 3058
Fragments arranged by size:
3058 439 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1365 2686 2740 4224 4389 50
Size: 1315 1321 54 1484 165 55
Fragments arranged by size:
1484 1321 1315 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 195 2392 2573 2782 195
Size: 2197 181 209 1807
Fragments arranged by size:
2197 1807 209 181
BssHII G'CGCG_C
Cuts at: 252 252
Size: 4394
BssSI C'ACGA_G
Cuts at: 679 1339 2665 3023 679
Size: 660 1326 358 2050
Fragments arranged by size:
2050 1326 660 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 110 2755 110
Size: 2645 1749
BstYI r'GATC_y
Cuts at: 1 341 569 1419 1886 1909 2244 2490 3502
Size: 340 228 850 467 23 335 246 1012
Cuts at: 3502 3588 3600 3946 1
Size: 86 12 346 449
Fragments arranged by size:
1012 850 467 449 346 340 335 246
228 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 323 323
Size: 4394
BtgI C'CryG_G
Cuts at: 125 125
Size: 4394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1047 2344 2525 2688 1047
Size: 1297 181 163 2753
Fragments arranged by size:
2753 1297 181 163
BtsI GCAGTG_nn'
Cuts at: 1637 1637
Size: 4394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 339 374 339
Size: 35 4359
DraI TTT'AAA
Cuts at: 1082 1393 1082
Size: 311 4083
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 128 1289 1979 2153 2544 2571 3916 128
Size: 1161 690 174 391 27 1345 606
Fragments arranged by size:
1345 1161 690 606 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 304 1096 1319 2417 2627 304
Size: 792 223 1098 210 2071
Fragments arranged by size:
2071 1098 792 223 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 214 2602 2912 3058 4204 166
Size: 48 2388 310 146 1146 356
Fragments arranged by size:
2388 1146 356 310 146 48
EcoICRI GAG'CTC
Cuts at: 336 1406 336
Size: 1070 3324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 878 975 992 1216 2219 2651 3398 3666
Size: 593 97 17 224 1003 432 747 268
Cuts at: 3666 285
Size: 1013
Fragments arranged by size:
1013 1003 747 593 432 268 224 97
17
EcoO109I rG'GnC_Cy
Cuts at: 1533 1533
Size: 4394
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1005 2413 1005
Size: 1408 2986
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 1828 2076 3098 4077 1828
Size: 248 1022 979 2145
Fragments arranged by size:
2145 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 485 517 1263 1295 2236 2266 2268 2298 485
Size: 32 746 32 941 30 2 30 2581
Fragments arranged by size:
2581 941 746 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 983 3065 3249 983
Size: 2082 184 2128
Fragments arranged by size:
2128 2082 184
MscI TGG'CCA
Cuts at: 130 1291 2155 130
Size: 1161 864 2369
Fragments arranged by size:
2369 1161 864
MslI CAynn'nnrTG
Cuts at: 550 2383 2510 550
Size: 1833 127 2434
Fragments arranged by size:
2434 1833 127
MspA1I CmG'CkG
Cuts at: 691 723 1221 1505 1819 2179 3192 3437 691
Size: 32 498 284 314 360 1013 245 1648
Fragments arranged by size:
1648 1013 498 360 314 284 245 32
NaeI GCC'GGC
Cuts at: 197 2575 197
Size: 2378 2016
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 125 125
Size: 4394
NgoMIV G'CCGG_C
Cuts at: 195 2573 195
Size: 2378 2016
NspI r_CATG'y
Cuts at: 1447 1947 2854 1447
Size: 500 907 2987
Fragments arranged by size:
2987 907 500
PasI CC'CwG_GG
Cuts at: 930 930
Size: 4394
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PfoI T'CCnGG_A
Cuts at: 572 659 572
Size: 87 4307
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 750 782 750
Size: 32 4362
PstI C_TGCA'G
Cuts at: 1441 2126 1441
Size: 685 3709
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 691 723 1819 2179 691
Size: 32 1096 360 2906
Fragments arranged by size:
2906 1096 360 32
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 338 1408 338
Size: 1070 3324
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 304 1096 2417 2627 304
Size: 792 1321 210 2071
Fragments arranged by size:
2071 1321 792 210
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 1437 2122 3115 3306 33
Size: 1404 685 993 191 1121
Fragments arranged by size:
1404 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1109 1109
Size: 4394
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 337 1549 2464 2956 3218 337
Size: 1212 915 492 262 1513
Fragments arranged by size:
1513 1212 915 492 262
SnaBI TAC'GTA
Cuts at: 1386 1386
Size: 4394
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
SspI AAT'ATT
Cuts at: 399 399
Size: 4394
StyI C'CwwG_G
Cuts at: 125 131 1528 1882 125
Size: 6 1397 354 2637
Fragments arranged by size:
2637 1397 354 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1247 1977 2110 2569 2778 1247
Size: 730 133 459 209 2863
Fragments arranged by size:
2863 730 459 209 133
TatI w'GTAC_w
Cuts at: 3909 3909
Size: 4394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1171 1203 3911 3943 1171
Size: 32 2708 32 1622
Fragments arranged by size:
2708 1622 32 32
Tth111I GACn'n_nGTC
Cuts at: 499 2191 499
Size: 1692 2702
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 138 420 1889 138
Size: 282 1469 2643
Fragments arranged by size:
2643 1469 282
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI
BsrGI BstEII BstXI BstZ17I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SgrAI SrfI StuI
SwaI XhoI XmnI ZraI