Restriction map for
pF1KSDA1580
(Circular) (Six-base) MAPSORT of: pF1KSDA1580.seq Check: 8485 from: 1 to: 5057
pF1KSDA1580 5057 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1886 1886
Size: 5057
AccI GT'mk_AC
Cuts at: 560 1571 1624 2095 560
Size: 1011 53 471 3522
Fragments arranged by size:
3522 1011 471 53
Acc65I G'GTAC_C
Cuts at: 2073 2073
Size: 5057
AclI AA'CG_TT
Cuts at: 4897 4897
Size: 5057
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 907 1244 1421 2882 3314 4061 4329 595
Size: 312 337 177 1461 432 747 268 1323
Fragments arranged by size:
1461 1323 747 432 337 312 268 177
AflIII A'CryG_T
Cuts at: 1399 2409 3513 4490 1399
Size: 1010 1104 977 1966
Fragments arranged by size:
1966 1104 1010 977
AgeI A'CCGG_T
Cuts at: 3445 3445
Size: 5057
AleI CACnn'nnGTG
Cuts at: 1564 1588 1564
Size: 24 5033
AlwNI CAG_nnn'CTG
Cuts at: 1654 2362 3929 1654
Size: 708 1567 2782
Fragments arranged by size:
2782 1567 708
ApaI G_GGCC'C
Cuts at: 189 189
Size: 5057
ApaLI G'TGCA_C
Cuts at: 3827 3827
Size: 5057
ApoI r'AATT_y
Cuts at: 635 1359 1968 2061 4410 4666 635
Size: 724 609 93 2349 256 1026
Fragments arranged by size:
2349 1026 724 609 256 93
AseI AT'TA_AT
Cuts at: 20 1832 20
Size: 1812 3245
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
AvaI C'yCGr_G
Cuts at: 2077 2077
Size: 5057
AvrII C'CTAG_G
Cuts at: 172 172
Size: 5057
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1259 1292 1259
Size: 33 5024
BamHI G'GATC_C
Cuts at: 1670 2082 1670
Size: 412 4645
BanI G'GyrC_C
Cuts at: 697 2073 2487 2735 2770 697
Size: 1376 414 248 35 2984
Fragments arranged by size:
2984 1376 414 248 35
BanII G_rGCy'C
Cuts at: 189 258 584 1157 1202 2071 3101 189
Size: 69 326 573 45 869 1030 2145
Fragments arranged by size:
2145 1030 869 573 326 69 45
BbeI G_GCGC'C
Cuts at: 2491 2739 2491
Size: 248 4809
BbsI GAAGACnn'nnnn_
Cuts at: 1702 1702
Size: 5057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1771 1805 1771
Size: 34 5023
BciVI GTATCCnnnnn_n'
Cuts at: 1004 2955 3716 5057 1004
Size: 1951 761 1341 1004
Fragments arranged by size:
1951 1341 1004 761
BclI T'GATC_A
Cuts at: 2577 2577
Size: 5057
BglII A'GATC_T
Cuts at: 2572 4609 2572
Size: 2037 3020
BlpI GC'TnA_GC
Cuts at: 1060 1238 2169 1060
Size: 178 931 3948
Fragments arranged by size:
3948 931 178
Bme1580I G_kGCm'C
Cuts at: 189 1110 2682 2775 3831 189
Size: 921 1572 93 1056 1415
Fragments arranged by size:
1572 1415 1056 921 93
BmrI ACTGGGnnnn_n'
Cuts at: 1584 1642 2269 2350 2685 4829 1584
Size: 58 627 81 335 2144 1812
Fragments arranged by size:
2144 1812 627 335 81 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3424 3424
Size: 5057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1547
Size: 5057
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 741 2233 3112 3604 3902 441
Size: 300 1492 879 492 298 1596
Fragments arranged by size:
1596 1492 879 492 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 1573 4761 1573
Size: 3188 1869
BsaAI yAC'GTr
Cuts at: 2049 2410 2049
Size: 361 4696
BsaBI GATnn'nnATC
Cuts at: 6 2571 4608 6
Size: 2565 2037 455
Fragments arranged by size:
2565 2037 455
BsaHI Gr'CG_yC
Cuts at: 2488 2736 3438 4934 2488
Size: 248 702 1496 2611
Fragments arranged by size:
2611 1496 702 248
BsaWI w'CCGG_w
Cuts at: 336 1185 2246 2767 3445 3719 3866 4277 336
Size: 849 1061 521 678 274 147 411 1116
Fragments arranged by size:
1116 1061 849 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 542 571 572 601 1455 1485 542
Size: 29 1 29 854 30 4114
Fragments arranged by size:
4114 854 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1112 1112
Size: 5057
BseYI C'CCAG_C
Cuts at: 2482 3240 3817 4313 2482
Size: 758 577 496 3226
Fragments arranged by size:
3226 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2599 2645 3853 104
Size: 2495 46 1208 1308
Fragments arranged by size:
2495 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 280 584 1157 1343 1618 1907 2071 2849 3039
Size: 304 573 186 275 289 164 778 190
Cuts at: 3039 3831 280
Size: 792 1506
Fragments arranged by size:
1506 792 778 573 304 289 275 190
186 164
BsmI GAATG_Cn'
Cuts at: 912 912
Size: 5057
BsmBI CGTCTCn'nnnn_
Cuts at: 1523 1523
Size: 5057
Bsp1286I G_dGCh'C
Cuts at: 189 258 280 584 1110 1157 1202 1343 1618
Size: 69 22 304 526 47 45 141 275
Cuts at: 1618 1907 2071 2682 2775 2849 3039 3101 3831
Size: 289 164 611 93 74 190 62 730
Cuts at: 3831 189
Size: 1415
Fragments arranged by size:
1415 730 611 526 304 289 275 190
164 141 93 74 69 62 47 45
22
BspEI T'CCGG_A
Cuts at: 336 4277 336
Size: 3941 1116
BspHI T'CATG_A
Cuts at: 975 1304 1691 4233 975
Size: 329 387 2542 1799
Fragments arranged by size:
2542 1799 387 329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 271 332 1886 2107 2321 2623 3004 271
Size: 61 1554 221 214 302 381 2324
Fragments arranged by size:
2324 1554 381 302 221 214 61
BsrBI CCG'CTC
Cuts at: 50 777 1008 3349 3403 4887 5052 50
Size: 727 231 2341 54 1484 165 55
Fragments arranged by size:
2341 1484 727 231 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2969 2969
Size: 5057
BsrFI r'CCGG_y
Cuts at: 3055 3236 3445 3055
Size: 181 209 4667
Fragments arranged by size:
4667 209 181
BsrGI T'GTAC_A
Cuts at: 1394 2028 1394
Size: 634 4423
BssSI C'ACGA_G
Cuts at: 3328 3686 3328
Size: 358 4699
BstAPI GCAn_nnn'nTGC
Cuts at: 2434 2434
Size: 5057
BstBI TT'CG_AA
Cuts at: 110 3418 110
Size: 3308 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1566 1566
Size: 5057
BstYI r'GATC_y
Cuts at: 1 798 1670 2082 2549 2572 2907 3153 4165
Size: 797 872 412 467 23 335 246 1012
Cuts at: 4165 4251 4263 4609 1
Size: 86 12 346 449
Fragments arranged by size:
1012 872 797 467 449 412 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 561 561
Size: 5057
BtgI C'CryG_G
Cuts at: 1865 1865
Size: 5057
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3007 3188 3351 3007
Size: 181 163 4713
Fragments arranged by size:
4713 181 163
BtsI GCAGTG_nn'
Cuts at: 1754 2300 1754
Size: 546 4511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 850 885 850
Size: 35 5022
DraI TTT'AAA
Cuts at: 2056 2056
Size: 5057
DrdI GACnn_nn'nnGTC
Cuts at: 1216 2763 3621 1216
Size: 1547 858 2652
Fragments arranged by size:
2652 1547 858
EaeI y'GGCC_r
Cuts at: 1727 2642 2816 3207 3234 4579 1727
Size: 915 174 391 27 1345 2205
Fragments arranged by size:
2205 1345 915 391 174 27
EagI C'GGCC_G
Cuts at: 2642 2642
Size: 5057
EarI CTCTTCn'nnn_
Cuts at: 227 988 3080 3290 227
Size: 761 2092 210 1994
Fragments arranged by size:
2092 1994 761 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3265 3575 3721 4867 3265
Size: 310 146 1146 3455
Fragments arranged by size:
3455 1146 310 146
EcoICRI GAG'CTC
Cuts at: 582 1155 2069 582
Size: 573 914 3570
Fragments arranged by size:
3570 914 573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 907 1244 1421 1547 2882 3314 4061 4329
Size: 312 337 177 126 1335 432 747 268
Cuts at: 4329 595
Size: 1323
Fragments arranged by size:
1335 1323 747 432 337 312 268 177
126
EcoNI CCTnn'n_nnAGG
Cuts at: 328 328
Size: 5057
EcoO109I rG'GnC_Cy
Cuts at: 169 185 186 1555 2196 169
Size: 16 1 1369 641 3030
Fragments arranged by size:
3030 1369 641 16 1
EcoRI G'AATT_C
Cuts at: 635 2061 635
Size: 1426 3631
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 159 632 3076 159
Size: 473 2444 2140
Fragments arranged by size:
2444 2140 473
FspI TGC'GCA
Cuts at: 2838 2838
Size: 5057
HaeII r_GCGC'y
Cuts at: 2491 2739 3761 4740 2491
Size: 248 1022 979 2808
Fragments arranged by size:
2808 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 132 164 331 363 1584 1616 2899 2929 2931
Size: 32 167 32 1221 32 1283 30 2
Cuts at: 2931 2961 132
Size: 30 2228
Fragments arranged by size:
2228 1283 1221 167 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1572 1625 1959 2096 1572
Size: 53 334 137 4533
Fragments arranged by size:
4533 334 137 53
HpaI GTT'AAC
Cuts at: 1959 1959
Size: 5057
KasI G'GCGC_C
Cuts at: 2487 2735 2487
Size: 248 4809
KpnI G_GTAC'C
Cuts at: 2077 2077
Size: 5057
MluI A'CGCG_T
Cuts at: 4490 4490
Size: 5057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 740 3728 3912 740
Size: 2988 184 1885
Fragments arranged by size:
2988 1885 184
MscI TGG'CCA
Cuts at: 1729 2818 1729
Size: 1089 3968
MslI CAynn'nnrTG
Cuts at: 400 1402 1564 1588 1648 1738 1864 3046 3173
Size: 1002 162 24 60 90 126 1182 127
Cuts at: 3173 400
Size: 2284
Fragments arranged by size:
2284 1182 1002 162 127 126 90 60
24
MspA1I CmG'CkG
Cuts at: 1064 1237 2168 2482 2842 3855 4100 1064
Size: 173 931 314 360 1013 245 2021
Fragments arranged by size:
2021 1013 931 360 314 245 173
NaeI GCC'GGC
Cuts at: 3238 3238
Size: 5057
NarI GG'CG_CC
Cuts at: 2488 2736 2488
Size: 248 4809
NcoI C'CATG_G
Cuts at: 1865 1865
Size: 5057
NgoMIV G'CCGG_C
Cuts at: 3236 3236
Size: 5057
NspI r_CATG'y
Cuts at: 1178 1395 1403 2110 2610 3517 1178
Size: 217 8 707 500 907 2718
Fragments arranged by size:
2718 907 707 500 217 8
PciI A'CATG_T
Cuts at: 1399 3513 1399
Size: 2114 2943
PmeI GTTT'AAAC
Cuts at: 2056 2056
Size: 5057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 4196
Size: 32 5025
PpuMI rG'GwC_Cy
Cuts at: 169 1555 169
Size: 1386 3671
PshAI GACnn'nnGTC
Cuts at: 527 527
Size: 5057
PspOMI G'GGCC_C
Cuts at: 185 185
Size: 5057
PstI C_TGCA'G
Cuts at: 286 1209 1461 1749 2104 2789 286
Size: 923 252 288 355 685 2554
Fragments arranged by size:
2554 923 685 355 288 252
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5057
PvuII CAG'CTG
Cuts at: 1064 1237 2482 2842 1064
Size: 173 1245 360 3279
Fragments arranged by size:
3279 1245 360 173
RsrII CG'GwC_CG
Cuts at: 3252 3252
Size: 5057
SacI G_AGCT'C
Cuts at: 584 1157 2071 584
Size: 573 914 3570
Fragments arranged by size:
3570 914 573
SalI G'TCGA_C
Cuts at: 1570 1623 2094 1570
Size: 53 471 4533
Fragments arranged by size:
4533 471 53
SanDI GG'GwC_CC
Cuts at: 1555 1555
Size: 5057
SapI GCTCTTCn'nnn_
Cuts at: 227 3080 3290 227
Size: 2853 210 1994
Fragments arranged by size:
2853 1994 210
SbfI CC_TGCA'GG
Cuts at: 2104 2104
Size: 5057
ScaI AGT'ACT
Cuts at: 4574 4574
Size: 5057
SfcI C'TryA_G
Cuts at: 33 282 439 1205 1457 1745 2100 2785 3778
Size: 249 157 766 252 288 355 685 993
Cuts at: 3778 3969 33
Size: 191 1121
Fragments arranged by size:
1121 993 766 685 355 288 252 249
191 157
SfoI GGC'GCC
Cuts at: 2489 2737 2489
Size: 248 4809
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5057
SmaI CCC'GGG
Cuts at: 2079 2079
Size: 5057
SmlI C'TyrA_G
Cuts at: 420 720 2212 3127 3619 3881 420
Size: 300 1492 915 492 262 1596
Fragments arranged by size:
1596 1492 915 492 300 262
SnaBI TAC'GTA
Cuts at: 2049 2049
Size: 5057
SpeI A'CTAG_T
Cuts at: 67 930 67
Size: 863 4194
SphI G_CATG'C
Cuts at: 2110 2610 2110
Size: 500 4557
StuI AGG'CCT
Cuts at: 831 831
Size: 5057
StyI C'CwwG_G
Cuts at: 172 1030 1865 2191 2545 172
Size: 858 835 326 354 2684
Fragments arranged by size:
2684 858 835 354 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1706 1878 2640 2773 3232 3441 1706
Size: 172 762 133 459 209 3322
Fragments arranged by size:
3322 762 459 209 172 133
TatI w'GTAC_w
Cuts at: 1394 2028 4572 1394
Size: 634 2544 1879
Fragments arranged by size:
2544 1879 634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4574 4606 4574
Size: 32 5025
Tth111I GACn'n_nGTC
Cuts at: 2854 2854
Size: 5057
XbaI T'CTAG_A
Cuts at: 1002 2088 1002
Size: 1086 3971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1571 1737 2552 1571
Size: 166 815 4076
Fragments arranged by size:
4076 815 166
XmaI C'CCGG_G
Cuts at: 2077 2077
Size: 5057
XmnI GAAnn'nnTTC
Cuts at: 409 639 1168 1636 409
Size: 230 529 468 3830
Fragments arranged by size:
3830 529 468 230
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI BbvCI
BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI BssHII
BstEII Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI