Restriction map for
pF1KSDA1581
(Circular) (Six-base) MAPSORT of: pF1KSDA1581.seq Check: 2819 from: 1 to: 4613
pF1KSDA1581 4613 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1478 1651 1478
Size: 173 4440
Acc65I G'GTAC_C
Cuts at: 1629 1629
Size: 4613
AclI AA'CG_TT
Cuts at: 4453 4453
Size: 4613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2438 2870 3617 3885 1327
Size: 1111 432 747 268 2055
Fragments arranged by size:
2055 1111 747 432 268
AflIII A'CryG_T
Cuts at: 1166 1965 3069 4046 1166
Size: 799 1104 977 1733
Fragments arranged by size:
1733 1104 977 799
AgeI A'CCGG_T
Cuts at: 3001 3001
Size: 4613
AlwNI CAG_nnn'CTG
Cuts at: 1918 3485 1918
Size: 1567 3046
ApaLI G'TGCA_C
Cuts at: 1393 3383 1393
Size: 1990 2623
ApoI r'AATT_y
Cuts at: 133 1617 3966 4222 133
Size: 1484 2349 256 524
Fragments arranged by size:
2349 1484 524 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4613
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
AvaI C'yCGr_G
Cuts at: 914 1633 914
Size: 719 3894
BamHI G'GATC_C
Cuts at: 1638 1638
Size: 4613
BanI G'GyrC_C
Cuts at: 177 1629 2043 2291 2326 177
Size: 1452 414 248 35 2464
Fragments arranged by size:
2464 1452 414 248 35
BanII G_rGCy'C
Cuts at: 692 1420 1627 2657 692
Size: 728 207 1030 2648
Fragments arranged by size:
2648 1030 728 207
BbeI G_GCGC'C
Cuts at: 2047 2295 2047
Size: 248 4365
BbsI GAAGACnn'nnnn_
Cuts at: 1535 1535
Size: 4613
BciVI GTATCCnnnnn_n'
Cuts at: 448 1034 2511 3272 4613 448
Size: 586 1477 761 1341 448
Fragments arranged by size:
1477 1341 761 586 448
BclI T'GATC_A
Cuts at: 2133 2133
Size: 4613
BglI GCCn_nnn'nGGC
Cuts at: 946 946
Size: 4613
BglII A'GATC_T
Cuts at: 2128 4165 2128
Size: 2037 2576
BlpI GC'TnA_GC
Cuts at: 838 1725 838
Size: 887 3726
Bme1580I G_kGCm'C
Cuts at: 182 1397 2238 2331 3387 182
Size: 1215 841 93 1056 1408
Fragments arranged by size:
1408 1215 1056 841 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1825 1906 2241 4385 1252
Size: 573 81 335 2144 1480
Fragments arranged by size:
2144 1480 573 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 4613
Bpu10I CC'TnA_GC
Cuts at: 638 638
Size: 4613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1789 2668 3160 3458 1789
Size: 879 492 298 2944
Fragments arranged by size:
2944 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4317 4317
Size: 4613
BsaAI yAC'GTr
Cuts at: 1605 1966 1605
Size: 361 4252
BsaBI GATnn'nnATC
Cuts at: 6 1189 2127 4164 6
Size: 1183 938 2037 455
Fragments arranged by size:
2037 1183 938 455
BsaHI Gr'CG_yC
Cuts at: 2044 2292 2994 4490 2044
Size: 248 702 1496 2167
Fragments arranged by size:
2167 1496 702 248
BsaWI w'CCGG_w
Cuts at: 167 1481 1802 2323 3001 3275 3422 3833 167
Size: 1314 321 521 678 274 147 411 947
Fragments arranged by size:
1314 947 678 521 411 321 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1495 1525 1495
Size: 30 4583
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1576 1606 1576
Size: 30 4583
BseYI C'CCAG_C
Cuts at: 807 1048 1330 2038 2796 3373 3869 807
Size: 241 282 708 758 577 496 1551
Fragments arranged by size:
1551 758 708 577 496 282 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 723 460
Size: 263 4350
BsiEI CG_ry'CG
Cuts at: 104 2155 2201 3409 104
Size: 2051 46 1208 1308
Fragments arranged by size:
2051 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1069 1397 1627 2405 2595 3387 1069
Size: 328 230 778 190 792 2295
Fragments arranged by size:
2295 792 778 328 230 190
BsmI GAATG_Cn'
Cuts at: 575 685 575
Size: 110 4503
Bsp1286I G_dGCh'C
Cuts at: 182 692 1069 1397 1420 1627 2238 2331 2405
Size: 510 377 328 23 207 611 93 74
Cuts at: 2405 2595 2657 3387 182
Size: 190 62 730 1408
Fragments arranged by size:
1408 730 611 510 377 328 207 190
93 74 62 23
BspEI T'CCGG_A
Cuts at: 3833 3833
Size: 4613
BspHI T'CATG_A
Cuts at: 299 3789 299
Size: 3490 1123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1663 1877 2179 2560 1663
Size: 214 302 381 3716
Fragments arranged by size:
3716 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2905 2959 4443 4608 50
Size: 2855 54 1484 165 55
Fragments arranged by size:
2855 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 236 560 2525 236
Size: 324 1965 2324
Fragments arranged by size:
2324 1965 324
BsrFI r'CCGG_y
Cuts at: 2611 2792 3001 2611
Size: 181 209 4223
Fragments arranged by size:
4223 209 181
BsrGI T'GTAC_A
Cuts at: 716 716
Size: 4613
BssSI C'ACGA_G
Cuts at: 1068 1369 2884 3242 1068
Size: 301 1515 358 2439
Fragments arranged by size:
2439 1515 358 301
BstAPI GCAn_nnn'nTGC
Cuts at: 1990 1990
Size: 4613
BstBI TT'CG_AA
Cuts at: 110 2974 110
Size: 2864 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 998 1371 998
Size: 373 4240
BstYI r'GATC_y
Cuts at: 1 1241 1638 2105 2128 2463 2709 3721 3807
Size: 1240 397 467 23 335 246 1012 86
Cuts at: 3807 3819 4165 1
Size: 12 346 449
Fragments arranged by size:
1240 1012 467 449 397 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 1451 1517 125
Size: 1326 66 3221
Fragments arranged by size:
3221 1326 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 396 1344 1446 2563 2744 2907 396
Size: 948 102 1117 181 163 2102
Fragments arranged by size:
2102 1117 948 181 163 102
BtsI GCAGTG_nn'
Cuts at: 956 1856 956
Size: 900 3713
DraI TTT'AAA
Cuts at: 1612 1612
Size: 4613
DraIII CAC_nnn'GTG
Cuts at: 483 749 1292 483
Size: 266 543 3804
Fragments arranged by size:
3804 543 266
DrdI GACnn_nn'nnGTC
Cuts at: 2319 3177 2319
Size: 858 3755
EaeI y'GGCC_r
Cuts at: 272 869 1514 2198 2372 2763 2790 4135 272
Size: 597 645 684 174 391 27 1345 750
Fragments arranged by size:
1345 750 684 645 597 391 174 27
EagI C'GGCC_G
Cuts at: 2198 2198
Size: 4613
EarI CTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2821 3131 3277 4423 2821
Size: 310 146 1146 3011
Fragments arranged by size:
3011 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1625 1625
Size: 4613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1327 2438 2870 3617 3885 1327
Size: 1111 432 747 268 2055
Fragments arranged by size:
2055 1111 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 316 515 918 1752 316
Size: 199 403 834 3177
Fragments arranged by size:
3177 834 403 199
EcoRI G'AATT_C
Cuts at: 1617 1617
Size: 4613
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1125 1332 2632 1125
Size: 207 1300 3106
Fragments arranged by size:
3106 1300 207
FspI TGC'GCA
Cuts at: 2394 2394
Size: 4613
HaeII r_GCGC'y
Cuts at: 145 2047 2295 3317 4296 145
Size: 1902 248 1022 979 462
Fragments arranged by size:
1902 1022 979 462 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 870 902 1266 1298 1495 1527 2455 2485 2487
Size: 32 364 32 197 32 928 30 2
Cuts at: 2487 2517 870
Size: 30 2966
Fragments arranged by size:
2966 928 364 197 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 900 1004 1652 900
Size: 104 648 3861
Fragments arranged by size:
3861 648 104
KasI G'GCGC_C
Cuts at: 2043 2291 2043
Size: 248 4365
KpnI G_GTAC'C
Cuts at: 1633 1633
Size: 4613
MluI A'CGCG_T
Cuts at: 4046 4046
Size: 4613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1579 3284 3468 1579
Size: 1705 184 2724
Fragments arranged by size:
2724 1705 184
MscI TGG'CCA
Cuts at: 274 871 2374 274
Size: 597 1503 2513
Fragments arranged by size:
2513 1503 597
MslI CAynn'nnrTG
Cuts at: 1198 2602 2729 1198
Size: 1404 127 3082
Fragments arranged by size:
3082 1404 127
MspA1I CmG'CkG
Cuts at: 1724 2038 2398 3411 3656 1724
Size: 314 360 1013 245 2681
Fragments arranged by size:
2681 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2794 2794
Size: 4613
NarI GG'CG_CC
Cuts at: 2044 2292 2044
Size: 248 4365
NcoI C'CATG_G
Cuts at: 125 1451 125
Size: 1326 3287
NgoMIV G'CCGG_C
Cuts at: 2792 2792
Size: 4613
NspI r_CATG'y
Cuts at: 990 1551 1666 2166 3073 990
Size: 561 115 500 907 2530
Fragments arranged by size:
2530 907 561 500 115
PasI CC'CwG_GG
Cuts at: 183 1407 183
Size: 1224 3389
PciI A'CATG_T
Cuts at: 3069 3069
Size: 4613
PmeI GTTT'AAAC
Cuts at: 1612 1612
Size: 4613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4581
PpuMI rG'GwC_Cy
Cuts at: 316 918 316
Size: 602 4011
PstI C_TGCA'G
Cuts at: 939 1660 2345 939
Size: 721 685 3207
Fragments arranged by size:
3207 721 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4613
PvuII CAG'CTG
Cuts at: 2038 2398 2038
Size: 360 4253
RsrII CG'GwC_CG
Cuts at: 2808 2808
Size: 4613
SacI G_AGCT'C
Cuts at: 1627 1627
Size: 4613
SalI G'TCGA_C
Cuts at: 1650 1650
Size: 4613
SanDI GG'GwC_CC
Cuts at: 316 316
Size: 4613
SapI GCTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
SbfI CC_TGCA'GG
Cuts at: 1660 1660
Size: 4613
ScaI AGT'ACT
Cuts at: 4130 4130
Size: 4613
SfcI C'TryA_G
Cuts at: 33 935 1656 2341 3334 3525 33
Size: 902 721 685 993 191 1121
Fragments arranged by size:
1121 993 902 721 685 191
SfoI GGC'GCC
Cuts at: 2045 2293 2045
Size: 248 4365
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
SmaI CCC'GGG
Cuts at: 916 1635 916
Size: 719 3894
SmlI C'TyrA_G
Cuts at: 1768 2683 3175 3437 1768
Size: 915 492 262 2944
Fragments arranged by size:
2944 915 492 262
SnaBI TAC'GTA
Cuts at: 1605 1605
Size: 4613
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4613
SphI G_CATG'C
Cuts at: 1666 2166 1666
Size: 500 4113
StuI AGG'CCT
Cuts at: 223 223
Size: 4613
StyI C'CwwG_G
Cuts at: 125 921 1451 1747 2101 125
Size: 796 530 296 354 2637
Fragments arranged by size:
2637 796 530 354 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1176 2196 2329 2788 2997 1176
Size: 1020 133 459 209 2792
Fragments arranged by size:
2792 1020 459 209 133
TatI w'GTAC_w
Cuts at: 716 4128 716
Size: 3412 1201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4130 4162 4130
Size: 32 4581
Tth111I GACn'n_nGTC
Cuts at: 2410 2410
Size: 4613
XbaI T'CTAG_A
Cuts at: 1644 1644
Size: 4613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 750 948 975 1056 2108 282
Size: 468 198 27 81 1052 2787
Fragments arranged by size:
2787 1052 468 198 81 27
XmaI C'CCGG_G
Cuts at: 914 1633 914
Size: 719 3894
XmnI GAAnn'nnTTC
Cuts at: 307 1315 307
Size: 1008 3605
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BpmI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SrfI SspI SwaI XhoI ZraI