Restriction map for
pF1KSDA1585
(Circular) (Six-base) MAPSORT of: pF1KSDA1585.seq Check: 2592 from: 1 to: 4868
pF1KSDA1585 4868 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 316 316
Size: 4868
AccI GT'mk_AC
Cuts at: 1906 1906
Size: 4868
Acc65I G'GTAC_C
Cuts at: 1884 1884
Size: 4868
AclI AA'CG_TT
Cuts at: 4708 4708
Size: 4868
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2693 3125 3872 4140 2693
Size: 432 747 268 3421
Fragments arranged by size:
3421 747 432 268
AflIII A'CryG_T
Cuts at: 2220 3324 4301 2220
Size: 1104 977 2787
Fragments arranged by size:
2787 1104 977
AgeI A'CCGG_T
Cuts at: 3256 3256
Size: 4868
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1747 1747
Size: 4868
AlwNI CAG_nnn'CTG
Cuts at: 2173 3740 2173
Size: 1567 3301
ApaLI G'TGCA_C
Cuts at: 3638 3638
Size: 4868
ApoI r'AATT_y
Cuts at: 1262 1872 4221 4477 1262
Size: 610 2349 256 1653
Fragments arranged by size:
2349 1653 610 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4868
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
AvaI C'yCGr_G
Cuts at: 1888 1888
Size: 4868
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1728 1761 1728
Size: 33 4835
BamHI G'GATC_C
Cuts at: 1369 1893 1369
Size: 524 4344
BanI G'GyrC_C
Cuts at: 1884 2298 2546 2581 1884
Size: 414 248 35 4171
Fragments arranged by size:
4171 414 248 35
BanII G_rGCy'C
Cuts at: 1882 2912 1882
Size: 1030 3838
BbeI G_GCGC'C
Cuts at: 2302 2550 2302
Size: 248 4620
BbvCI CC'TCA_GC
Cuts at: 670 670
Size: 4868
BciVI GTATCCnnnnn_n'
Cuts at: 1029 2766 3527 4868 1029
Size: 1737 761 1341 1029
Fragments arranged by size:
1737 1341 1029 761
BclI T'GATC_A
Cuts at: 2388 2388
Size: 4868
BglII A'GATC_T
Cuts at: 2383 4420 2383
Size: 2037 2831
BlpI GC'TnA_GC
Cuts at: 1980 1980
Size: 4868
Bme1580I G_kGCm'C
Cuts at: 2493 2586 3642 2493
Size: 93 1056 3719
Fragments arranged by size:
3719 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1044 1231 1774 2080 2161 2496 4640 1044
Size: 187 543 306 81 335 2144 1272
Fragments arranged by size:
2144 1272 543 335 306 187 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3235 3235
Size: 4868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 1675 932
Size: 743 4125
Bpu10I CC'TnA_GC
Cuts at: 670 670
Size: 4868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 2044 2923 3415 3713 620
Size: 1424 879 492 298 1775
Fragments arranged by size:
1775 1424 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 188 4572 188
Size: 4384 484
BsaAI yAC'GTr
Cuts at: 1860 2221 1860
Size: 361 4507
BsaBI GATnn'nnATC
Cuts at: 6 1155 2382 4419 6
Size: 1149 1227 2037 455
Fragments arranged by size:
2037 1227 1149 455
BsaHI Gr'CG_yC
Cuts at: 2299 2547 3249 4745 2299
Size: 248 702 1496 2422
Fragments arranged by size:
2422 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2057 2578 3256 3530 3677 4088 2057
Size: 521 678 274 147 411 2837
Fragments arranged by size:
2837 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 562 562
Size: 4868
BseYI C'CCAG_C
Cuts at: 2293 3051 3628 4124 2293
Size: 758 577 496 3037
Fragments arranged by size:
3037 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1073 1073
Size: 4868
BsiEI CG_ry'CG
Cuts at: 104 2410 2456 3664 104
Size: 2306 46 1208 1308
Fragments arranged by size:
2306 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 179 1882 2660 2850 3642 179
Size: 1703 778 190 792 1405
Fragments arranged by size:
1703 1405 792 778 190
BsmI GAATG_Cn'
Cuts at: 144 144
Size: 4868
Bsp1286I G_dGCh'C
Cuts at: 179 1882 2493 2586 2660 2850 2912 3642 179
Size: 1703 611 93 74 190 62 730 1405
Fragments arranged by size:
1703 1405 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4088 4088
Size: 4868
BspHI T'CATG_A
Cuts at: 1359 4044 1359
Size: 2685 2183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 316 1918 2132 2434 2815 316
Size: 1602 214 302 381 2369
Fragments arranged by size:
2369 1602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3160 3214 4698 4863 50
Size: 3110 54 1484 165 55
Fragments arranged by size:
3110 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 674 1724 2780 674
Size: 1050 1056 2762
Fragments arranged by size:
2762 1056 1050
BsrFI r'CCGG_y
Cuts at: 2866 3047 3256 2866
Size: 181 209 4478
Fragments arranged by size:
4478 209 181
BsrGI T'GTAC_A
Cuts at: 1108 1481 1108
Size: 373 4495
BssSI C'ACGA_G
Cuts at: 3139 3497 3139
Size: 358 4510
BstAPI GCAn_nnn'nTGC
Cuts at: 1592 2245 1592
Size: 653 4215
BstBI TT'CG_AA
Cuts at: 110 3229 110
Size: 3119 1749
BstYI r'GATC_y
Cuts at: 1 282 813 1369 1893 2360 2383 2718 2964
Size: 281 531 556 524 467 23 335 246
Cuts at: 2964 3976 4062 4074 4420 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 556 531 524 467 449 346 335
281 246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2818 2999 3162 2818
Size: 181 163 4524
Fragments arranged by size:
4524 181 163
BtsI GCAGTG_nn'
Cuts at: 2111 2111
Size: 4868
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 858 893 858
Size: 35 4833
DraI TTT'AAA
Cuts at: 1867 1867
Size: 4868
DrdI GACnn_nn'nnGTC
Cuts at: 2574 3432 2574
Size: 858 4010
EaeI y'GGCC_r
Cuts at: 1634 2453 2627 3018 3045 4390 1634
Size: 819 174 391 27 1345 2112
Fragments arranged by size:
2112 1345 819 391 174 27
EagI C'GGCC_G
Cuts at: 2453 2453
Size: 4868
EarI CTCTTCn'nnn_
Cuts at: 1274 2891 3101 1274
Size: 1617 210 3041
Fragments arranged by size:
3041 1617 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3076 3386 3532 4678 3076
Size: 310 146 1146 3266
Fragments arranged by size:
3266 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1880 1880
Size: 4868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 1675 2693 3125 3872 4140 932
Size: 743 1018 432 747 268 1660
Fragments arranged by size:
1660 1018 747 743 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1775 2007 1775
Size: 232 4636
EcoRI G'AATT_C
Cuts at: 1872 1872
Size: 4868
EcoRV GAT'ATC
Cuts at: 882 882
Size: 4868
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2887 2887
Size: 4868
FspI TGC'GCA
Cuts at: 2649 2649
Size: 4868
HaeII r_GCGC'y
Cuts at: 2302 2550 3572 4551 2302
Size: 248 1022 979 2619
Fragments arranged by size:
2619 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 802 834 1428 1460 2710 2740 2742 2772 802
Size: 32 594 32 1250 30 2 30 2898
Fragments arranged by size:
2898 1250 594 32 32 30 30 2
HincII GTy'rAC
Cuts at: 495 798 1907 495
Size: 303 1109 3456
Fragments arranged by size:
3456 1109 303
KasI G'GCGC_C
Cuts at: 2298 2546 2298
Size: 248 4620
KpnI G_GTAC'C
Cuts at: 1888 1888
Size: 4868
MluI A'CGCG_T
Cuts at: 4301 4301
Size: 4868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 264 3539 3723 264
Size: 3275 184 1409
Fragments arranged by size:
3275 1409 184
MscI TGG'CCA
Cuts at: 1636 2629 1636
Size: 993 3875
MslI CAynn'nnrTG
Cuts at: 421 1364 1714 1729 2857 2984 421
Size: 943 350 15 1128 127 2305
Fragments arranged by size:
2305 1128 943 350 127 15
MspA1I CmG'CkG
Cuts at: 1979 2293 2653 3666 3911 1979
Size: 314 360 1013 245 2936
Fragments arranged by size:
2936 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3049 3049
Size: 4868
NarI GG'CG_CC
Cuts at: 2299 2547 2299
Size: 248 4620
NgoMIV G'CCGG_C
Cuts at: 3047 3047
Size: 4868
NspI r_CATG'y
Cuts at: 1553 1921 2421 3328 1553
Size: 368 500 907 3093
Fragments arranged by size:
3093 907 500 368
PciI A'CATG_T
Cuts at: 3324 3324
Size: 4868
PmeI GTTT'AAAC
Cuts at: 1867 1867
Size: 4868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 4007
Size: 32 4836
PpuMI rG'GwC_Cy
Cuts at: 1775 1775
Size: 4868
PsiI TTA'TAA
Cuts at: 716 716
Size: 4868
PstI C_TGCA'G
Cuts at: 889 1040 1915 2600 889
Size: 151 875 685 3157
Fragments arranged by size:
3157 875 685 151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4868
PvuII CAG'CTG
Cuts at: 2293 2653 2293
Size: 360 4508
RsrII CG'GwC_CG
Cuts at: 3063 3063
Size: 4868
SacI G_AGCT'C
Cuts at: 1882 1882
Size: 4868
SalI G'TCGA_C
Cuts at: 1905 1905
Size: 4868
SanDI GG'GwC_CC
Cuts at: 1775 1775
Size: 4868
SapI GCTCTTCn'nnn_
Cuts at: 2891 3101 2891
Size: 210 4658
SbfI CC_TGCA'GG
Cuts at: 1915 1915
Size: 4868
ScaI AGT'ACT
Cuts at: 944 4385 944
Size: 3441 1427
SfcI C'TryA_G
Cuts at: 33 366 885 1036 1277 1835 1911 2596 3589
Size: 333 519 151 241 558 76 685 993
Cuts at: 3589 3780 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 558 519 333 241 191
151 76
SfoI GGC'GCC
Cuts at: 2300 2548 2300
Size: 248 4620
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4868
SmaI CCC'GGG
Cuts at: 1890 1890
Size: 4868
SmlI C'TyrA_G
Cuts at: 635 2023 2938 3430 3692 635
Size: 1388 915 492 262 1811
Fragments arranged by size:
1811 1388 915 492 262
SnaBI TAC'GTA
Cuts at: 1860 1860
Size: 4868
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4868
SphI G_CATG'C
Cuts at: 1921 2421 1921
Size: 500 4368
SspI AAT'ATT
Cuts at: 750 750
Size: 4868
StuI AGG'CCT
Cuts at: 1681 1681
Size: 4868
StyI C'CwwG_G
Cuts at: 200 1682 2002 2356 200
Size: 1482 320 354 2712
Fragments arranged by size:
2712 1482 354 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 309 2451 2584 3043 3252 309
Size: 2142 133 459 209 1925
Fragments arranged by size:
2142 1925 459 209 133
TatI w'GTAC_w
Cuts at: 942 1108 1326 1481 1804 4383 942
Size: 166 218 155 323 2579 1427
Fragments arranged by size:
2579 1427 323 218 166 155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 615 647 4385 4417 615
Size: 32 3738 32 1066
Fragments arranged by size:
3738 1066 32 32
Tth111I GACn'n_nGTC
Cuts at: 2665 2665
Size: 4868
XbaI T'CTAG_A
Cuts at: 1899 1899
Size: 4868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1701 2363 1701
Size: 662 4206
XmaI C'CCGG_G
Cuts at: 1888 1888
Size: 4868
XmnI GAAnn'nnTTC
Cuts at: 1436 1436
Size: 4868
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BbsI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SrfI SwaI XhoI ZraI