Restriction map for
pF1KSDA1587
(Circular) (Six-base) MAPSORT of: pF1KSDA1587.seq Check: 7923 from: 1 to: 6010
pF1KSDA1587 6010 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 260 260
Size: 6010
AccI GT'mk_AC
Cuts at: 2606 3046 2606
Size: 440 5570
Acc65I G'GTAC_C
Cuts at: 600 2286 3024 600
Size: 1686 738 3586
Fragments arranged by size:
3586 1686 738
AclI AA'CG_TT
Cuts at: 5850 5850
Size: 6010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1600 2152 2976 3833 4265 5014 5282 1600
Size: 552 824 857 432 749 268 2328
Fragments arranged by size:
2328 857 824 749 552 432 268
AfeI AGC'GCT
Cuts at: 2770 2770
Size: 6010
AflIII A'CryG_T
Cuts at: 3360 4466 5443 3360
Size: 1106 977 3927
Fragments arranged by size:
3927 1106 977
AgeI A'CCGG_T
Cuts at: 4398 4398
Size: 6010
AleI CACnn'nnGTG
Cuts at: 361 433 505 577 649 685 721 757 793
Size: 72 72 72 72 36 36 36 36
Cuts at: 793 829 865 901 937 1009 1045 1081 1117
Size: 36 36 36 36 72 36 36 36
Cuts at: 1117 1153 1225 1261 1297 361
Size: 36 72 36 36 5074
Fragments arranged by size:
5074 72 72 72 72 72 72 36
36 36 36 36 36 36 36 36
36 36 36 36 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 379 451 595 631 667 739 775 811 847
Size: 72 144 36 36 72 36 36 36
Cuts at: 847 883 919 955 1027 1063 1073 1099 1135
Size: 36 36 36 72 36 10 26 36
Cuts at: 1135 1171 1243 1315 379
Size: 36 72 72 5074
Fragments arranged by size:
5074 144 72 72 72 72 72 36
36 36 36 36 36 36 36 36
36 36 26 10
AlwNI CAG_nnn'CTG
Cuts at: 197 1465 1629 2056 3313 4882 197
Size: 1268 164 427 1257 1569 1325
Fragments arranged by size:
1569 1325 1268 1257 427 164
ApaI G_GGCC'C
Cuts at: 316 352 1360 2095 316
Size: 36 1008 735 4231
Fragments arranged by size:
4231 1008 735 36
ApaLI G'TGCA_C
Cuts at: 498 4780 498
Size: 4282 1728
ApoI r'AATT_y
Cuts at: 144 1498 2126 2435 3012 5363 5619 144
Size: 1354 628 309 577 2351 256 535
Fragments arranged by size:
2351 1354 628 577 535 309 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6010
AvaI C'yCGr_G
Cuts at: 1359 1562 3028 1359
Size: 203 1466 4341
Fragments arranged by size:
4341 1466 203
AvrII C'CTAG_G
Cuts at: 1432 1432
Size: 6010
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2748 2781 2748
Size: 33 5977
BamHI G'GATC_C
Cuts at: 3033 3033
Size: 6010
BanI G'GyrC_C
Cuts at: 427 600 690 751 978 2286 3024 3438 3686
Size: 173 90 61 227 1308 738 414 248
Cuts at: 3686 3721 427
Size: 35 2716
Fragments arranged by size:
2716 1308 738 414 248 227 173 90
61 35
BanII G_rGCy'C
Cuts at: 316 352 1360 1528 1852 2095 2841 3022 4052
Size: 36 1008 168 324 243 746 181 1030
Cuts at: 4052 316
Size: 2274
Fragments arranged by size:
2274 1030 1008 746 324 243 181 168
36
BbeI G_GCGC'C
Cuts at: 3442 3690 3442
Size: 248 5762
BbvCI CC'TCA_GC
Cuts at: 344 2225 344
Size: 1881 4129
BciVI GTATCCnnnnn_n'
Cuts at: 3906 4669 6010 3906
Size: 763 1341 3906
Fragments arranged by size:
3906 1341 763
BclI T'GATC_A
Cuts at: 3528 3528
Size: 6010
BglI GCCn_nnn'nGGC
Cuts at: 1924 1924
Size: 6010
BglII A'GATC_T
Cuts at: 3523 5562 3523
Size: 2039 3971
BlpI GC'TnA_GC
Cuts at: 1613 3120 1613
Size: 1507 4503
Bme1580I G_kGCm'C
Cuts at: 316 352 502 695 983 1016 1360 1829 2095
Size: 36 150 193 288 33 344 469 266
Cuts at: 2095 2969 3633 3726 4784 316
Size: 874 664 93 1058 1542
Fragments arranged by size:
1542 1058 874 664 469 344 288 266
193 150 93 36 33
BmrI ACTGGGnnnn_n'
Cuts at: 443 1825 3220 3301 3636 5782 443
Size: 1382 1395 81 335 2146 671
Fragments arranged by size:
2146 1395 1382 671 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4375 4375
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 525 1765 1968 274
Size: 251 1240 203 4316
Fragments arranged by size:
4316 1240 251 203
Bpu10I CC'TnA_GC
Cuts at: 344 460 784 856 1036 2225 2650 2715 344
Size: 116 324 72 180 1189 425 65 3639
Fragments arranged by size:
3639 1189 425 324 180 116 72 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 372 2781 3184 4063 4557 4855 372
Size: 2409 403 879 494 298 1527
Fragments arranged by size:
2409 1527 879 494 403 298
BsaI GGTCTCn'nnnn_
Cuts at: 235 5714 235
Size: 5479 531
BsaAI yAC'GTr
Cuts at: 3000 3361 3000
Size: 361 5649
BsaBI GATnn'nnATC
Cuts at: 6 3522 5561 6
Size: 3516 2039 455
Fragments arranged by size:
3516 2039 455
BsaHI Gr'CG_yC
Cuts at: 257 3439 3687 4391 5887 257
Size: 3182 248 704 1496 380
Fragments arranged by size:
3182 1496 704 380 248
BsaWI w'CCGG_w
Cuts at: 1394 3197 3718 4398 4672 4819 5230 1394
Size: 1803 521 680 274 147 411 2174
Fragments arranged by size:
2174 1803 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2065 2095 2065
Size: 30 5980
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1153 2611 1153
Size: 1458 4552
BseYI C'CCAG_C
Cuts at: 197 271 2728 3433 4191 4770 5266 197
Size: 74 2457 705 758 579 496 941
Fragments arranged by size:
2457 941 758 705 579 496 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1069 1321 1502 1981 2404 2481 817
Size: 252 252 181 479 423 77 4346
Fragments arranged by size:
4346 479 423 252 252 181 77
BsiEI CG_ry'CG
Cuts at: 104 3550 3596 4806 104
Size: 3446 46 1210 1308
Fragments arranged by size:
3446 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 502 610 646 790 826 898 934 1042 1078
Size: 108 36 144 36 72 36 108 36
Cuts at: 1078 1114 1150 1222 1366 3022 3800 3990 4784
Size: 36 36 72 144 1656 778 190 794
Cuts at: 4784 502
Size: 1728
Fragments arranged by size:
1728 1656 794 778 190 144 144 108
108 72 72 36 36 36 36 36
36
Bsp1286I G_dGCh'C
Cuts at: 316 352 502 610 646 695 790 826 898
Size: 36 150 108 36 49 95 36 72
Cuts at: 898 934 983 1016 1042 1078 1114 1150 1222
Size: 36 49 33 26 36 36 36 72
Cuts at: 1222 1360 1366 1528 1829 1852 2095 2841 2969
Size: 138 6 162 301 23 243 746 128
Cuts at: 2969 3022 3633 3726 3800 3990 4052 4784 316
Size: 53 611 93 74 190 62 732 1542
Fragments arranged by size:
1542 746 732 611 301 243 190 162
150 138 128 108 95 93 74 72
72 62 53 49 49 36 36 36
36 36 36 36 33 26 23 6
BspEI T'CCGG_A
Cuts at: 5230 5230
Size: 6010
BspHI T'CATG_A
Cuts at: 5186 5186
Size: 6010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2634 2758 3058 3272 3574 3955 2634
Size: 124 300 214 302 381 4689
Fragments arranged by size:
4689 381 302 300 214 124
BsrBI CCG'CTC
Cuts at: 50 765 4300 4354 5840 6005 50
Size: 715 3535 54 1486 165 55
Fragments arranged by size:
3535 1486 715 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2939 3920 2939
Size: 981 5029
BsrFI r'CCGG_y
Cuts at: 906 4006 4187 4398 906
Size: 3100 181 211 2518
Fragments arranged by size:
3100 2518 211 181
BsrGI T'GTAC_A
Cuts at: 2567 2567
Size: 6010
BssSI C'ACGA_G
Cuts at: 4279 4639 4279
Size: 360 5650
BstAPI GCAn_nnn'nTGC
Cuts at: 1629 3385 1629
Size: 1756 4254
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1437 1437
Size: 6010
BstXI CCAn_nnnn'nTGG
Cuts at: 132 2342 2518 2909 132
Size: 2210 176 391 3233
Fragments arranged by size:
3233 2210 391 176
BstYI r'GATC_y
Cuts at: 1 284 2098 2160 3033 3500 3523 3858 4104
Size: 283 1814 62 873 467 23 335 246
Cuts at: 4104 5118 5204 5216 5562 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1814 1014 873 467 449 346 335 283
246 86 62 23 12
Bsu36I CC'TnA_GG
Cuts at: 1475 1784 1475
Size: 309 5701
BtgI C'CryG_G
Cuts at: 686 987 1451 686
Size: 301 464 5245
Fragments arranged by size:
5245 464 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3958 4139 4302 3958
Size: 181 163 5666
Fragments arranged by size:
5666 181 163
BtsI GCAGTG_nn'
Cuts at: 1055 3251 1055
Size: 2196 3814
DraI TTT'AAA
Cuts at: 3007 3007
Size: 6010
DraIII CAC_nnn'GTG
Cuts at: 739 739
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 3714 4574 3714
Size: 860 5150
EaeI y'GGCC_r
Cuts at: 908 2173 2451 2879 2911 3593 3767 4158 4185
Size: 1265 278 428 32 682 174 391 27
Cuts at: 4185 5532 908
Size: 1347 1386
Fragments arranged by size:
1386 1347 1265 682 428 391 278 174
32 27
EagI C'GGCC_G
Cuts at: 3593 3593
Size: 6010
EarI CTCTTCn'nnn_
Cuts at: 1838 4031 4241 1838
Size: 2193 210 3607
Fragments arranged by size:
3607 2193 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4216 4528 4674 5820 4216
Size: 312 146 1146 4406
Fragments arranged by size:
4406 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3020 3020
Size: 6010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 525 1600 1765 1968 2152 2976 3833 4265
Size: 251 1075 165 203 184 824 857 432
Cuts at: 4265 5014 5282 274
Size: 749 268 1002
Fragments arranged by size:
1075 1002 857 824 749 432 268 251
203 184 165
EcoO109I rG'GnC_Cy
Cuts at: 213 268 294 312 348 421 456 780 852
Size: 55 26 18 36 73 35 324 72
Cuts at: 852 1032 1356 1471 1489 1506 1819 2091 2258
Size: 180 324 115 18 17 313 272 167
Cuts at: 2258 2646 2830 3147 213
Size: 388 184 317 3076
Fragments arranged by size:
3076 388 324 324 317 313 272 184
180 167 115 73 72 55 36 35
26 18 18 17
EcoRI G'AATT_C
Cuts at: 144 1498 2435 3012 144
Size: 1354 937 577 3142
Fragments arranged by size:
3142 1354 937 577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4027 4027
Size: 6010
FspI TGC'GCA
Cuts at: 185 3789 185
Size: 3604 2406
HaeII r_GCGC'y
Cuts at: 395 2772 3442 3690 4714 5693 395
Size: 2377 670 248 1024 979 712
Fragments arranged by size:
2377 1024 979 712 670 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 953 985 2275 2307 3850 3880 3882 3912 953
Size: 32 1290 32 1543 30 2 30 3051
Fragments arranged by size:
3051 1543 1290 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3047 3047
Size: 6010
HindIII A'AGCT_T
Cuts at: 2652 2652
Size: 6010
KasI G'GCGC_C
Cuts at: 3438 3686 3438
Size: 248 5762
KpnI G_GTAC'C
Cuts at: 604 2290 3028 604
Size: 1686 738 3586
Fragments arranged by size:
3586 1686 738
MluI A'CGCG_T
Cuts at: 5443 5443
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4681 4865 4681
Size: 184 5826
MscI TGG'CCA
Cuts at: 2175 2881 2913 3769 2175
Size: 706 32 856 4416
Fragments arranged by size:
4416 856 706 32
MslI CAynn'nnrTG
Cuts at: 361 433 505 577 649 685 721 757 793
Size: 72 72 72 72 36 36 36 36
Cuts at: 793 829 865 901 937 1009 1045 1081 1117
Size: 36 36 36 36 72 36 36 36
Cuts at: 1117 1153 1225 1261 1297 2119 2340 2516 3997
Size: 36 72 36 36 822 221 176 1481
Cuts at: 3997 4124 361
Size: 127 2247
Fragments arranged by size:
2247 1481 822 221 176 127 72 72
72 72 72 72 36 36 36 36
36 36 36 36 36 36 36 36
36 36
MspA1I CmG'CkG
Cuts at: 197 238 343 989 1965 3119 3433 3793 4808
Size: 41 105 646 976 1154 314 360 1015
Cuts at: 4808 5053 197
Size: 245 1154
Fragments arranged by size:
1154 1154 1015 976 646 360 314 245
105 41
NaeI GCC'GGC
Cuts at: 908 4189 908
Size: 3281 2729
NarI GG'CG_CC
Cuts at: 3439 3687 3439
Size: 248 5762
NgoMIV G'CCGG_C
Cuts at: 906 4187 906
Size: 3281 2729
NruI TCG'CGA
Cuts at: 4371 4371
Size: 6010
NspI r_CATG'y
Cuts at: 2743 3061 3561 4470 2743
Size: 318 500 909 4283
Fragments arranged by size:
4283 909 500 318
PasI CC'CwG_GG
Cuts at: 264 264
Size: 6010
PciI A'CATG_T
Cuts at: 4466 4466
Size: 6010
PfoI T'CCnGG_A
Cuts at: 1661 1661
Size: 6010
PmeI GTTT'AAAC
Cuts at: 3007 3007
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 5149 5181 114
Size: 32 5003 32 943
Fragments arranged by size:
5003 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 268 421 1471 1506 1819 2646 2830 268
Size: 153 1050 35 313 827 184 3448
Fragments arranged by size:
3448 1050 827 313 184 153 35
PshAI GACnn'nnGTC
Cuts at: 2007 2007
Size: 6010
PspOMI G'GGCC_C
Cuts at: 312 348 1356 2091 312
Size: 36 1008 735 4231
Fragments arranged by size:
4231 1008 735 36
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2519 2551 2519
Size: 32 5978
PstI C_TGCA'G
Cuts at: 2283 2694 2755 3055 3740 2283
Size: 411 61 300 685 4553
Fragments arranged by size:
4553 685 411 300 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6010
PvuII CAG'CTG
Cuts at: 197 3433 3793 197
Size: 3236 360 2414
Fragments arranged by size:
3236 2414 360
RsrII CG'GwC_CG
Cuts at: 1321 4203 1321
Size: 2882 3128
SacI G_AGCT'C
Cuts at: 3022 3022
Size: 6010
SacII CC_GC'GG
Cuts at: 990 990
Size: 6010
SalI G'TCGA_C
Cuts at: 3045 3045
Size: 6010
SanDI GG'GwC_CC
Cuts at: 268 268
Size: 6010
SapI GCTCTTCn'nnn_
Cuts at: 4031 4241 4031
Size: 210 5800
SbfI CC_TGCA'GG
Cuts at: 3055 3055
Size: 6010
ScaI AGT'ACT
Cuts at: 5527 5527
Size: 6010
SexAI A'CCwGG_T
Cuts at: 735 735
Size: 6010
SfcI C'TryA_G
Cuts at: 33 1493 2043 2279 2690 2751 3051 3736 4731
Size: 1460 550 236 411 61 300 685 995
Cuts at: 4731 4922 33
Size: 191 1121
Fragments arranged by size:
1460 1121 995 685 550 411 300 236
191 61
SfoI GGC'GCC
Cuts at: 3440 3688 3440
Size: 248 5762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6010
SmaI CCC'GGG
Cuts at: 1564 3030 1564
Size: 1466 4544
SmlI C'TyrA_G
Cuts at: 387 2760 3163 4078 4572 4834 387
Size: 2373 403 915 494 262 1563
Fragments arranged by size:
2373 1563 915 494 403 262
SnaBI TAC'GTA
Cuts at: 3000 3000
Size: 6010
SpeI A'CTAG_T
Cuts at: 67 2529 67
Size: 2462 3548
SphI G_CATG'C
Cuts at: 3061 3561 3061
Size: 500 5510
SspI AAT'ATT
Cuts at: 1898 1898
Size: 6010
StuI AGG'CCT
Cuts at: 385 1447 2622 385
Size: 1062 1175 3773
Fragments arranged by size:
3773 1175 1062
StyI C'CwwG_G
Cuts at: 974 1432 1442 1919 3142 3496 974
Size: 458 10 477 1223 354 3488
Fragments arranged by size:
3488 1223 477 458 354 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 834 888 906 942 3591 3724 4183 4394 834
Size: 54 18 36 2649 133 459 211 2450
Fragments arranged by size:
2649 2450 459 211 133 54 36 18
TatI w'GTAC_w
Cuts at: 2441 2567 2921 5525 2441
Size: 126 354 2604 2926
Fragments arranged by size:
2926 2604 354 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1319 1351 5527 5559 1319
Size: 32 4176 32 1770
Fragments arranged by size:
4176 1770 32 32
Tth111I GACn'n_nGTC
Cuts at: 3805 3805
Size: 6010
XbaI T'CTAG_A
Cuts at: 3039 3039
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1449 1542 3503 1449
Size: 93 1961 3956
Fragments arranged by size:
3956 1961 93
XmaI C'CCGG_G
Cuts at: 1562 3028 1562
Size: 1466 4544
ZraI GAC'GTC
Cuts at: 258 258
Size: 6010
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NruI NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AloI AscI BbsI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BssHII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NsiI PacI PflMI PmlI PsiI
PspXI SfiI SgrAI SrfI SwaI XhoI XmnI