Restriction map for
pF1KSDA1589
(Circular) (Six-base) MAPSORT of: pF1KSDA1589.seq Check: 1149 from: 1 to: 5468
pF1KSDA1589 5468 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1417 1417
Size: 5468
AatII G_ACGT'C
Cuts at: 1954 1954
Size: 5468
AccI GT'mk_AC
Cuts at: 587 1534 2506 587
Size: 947 972 3549
Fragments arranged by size:
3549 972 947
Acc65I G'GTAC_C
Cuts at: 2484 2484
Size: 5468
AclI AA'CG_TT
Cuts at: 5308 5308
Size: 5468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1199 1329 1345 1795 3293 3725 4472 4740
Size: 190 130 16 450 1498 432 747 268
Cuts at: 4740 1009
Size: 1737
Fragments arranged by size:
1737 1498 747 450 432 268 190 130
16
AflII C'TTAA_G
Cuts at: 886 886
Size: 5468
AflIII A'CryG_T
Cuts at: 2820 3924 4901 2820
Size: 1104 977 3387
Fragments arranged by size:
3387 1104 977
AgeI A'CCGG_T
Cuts at: 1151 3856 1151
Size: 2705 2763
AlwNI CAG_nnn'CTG
Cuts at: 663 1104 1803 1929 2041 2773 4340 663
Size: 441 699 126 112 732 1567 1791
Fragments arranged by size:
1791 1567 732 699 441 126 112
ApaI G_GGCC'C
Cuts at: 1063 2059 1063
Size: 996 4472
ApaLI G'TGCA_C
Cuts at: 1090 4238 1090
Size: 3148 2320
ApoI r'AATT_y
Cuts at: 602 958 2472 4821 5077 602
Size: 356 1514 2349 256 993
Fragments arranged by size:
2349 1514 993 356 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5468
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
AvaI C'yCGr_G
Cuts at: 1302 2488 1302
Size: 1186 4282
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1712 1745 1712
Size: 33 5435
BamHI G'GATC_C
Cuts at: 2493 2493
Size: 5468
BanI G'GyrC_C
Cuts at: 398 1128 1298 2262 2484 2898 3146 3181 398
Size: 730 170 964 222 414 248 35 2685
Fragments arranged by size:
2685 964 730 414 248 222 170 35
BanII G_rGCy'C
Cuts at: 272 842 1010 1063 1111 2059 2482 3512 272
Size: 570 168 53 48 948 423 1030 2228
Fragments arranged by size:
2228 1030 948 570 423 168 53 48
BbeI G_GCGC'C
Cuts at: 2902 3150 2902
Size: 248 5220
BbsI GAAGACnn'nnnn_
Cuts at: 629 629
Size: 5468
BbvCI CC'TCA_GC
Cuts at: 1426 1426
Size: 5468
BciVI GTATCCnnnnn_n'
Cuts at: 1713 3366 4127 5468 1713
Size: 1653 761 1341 1713
Fragments arranged by size:
1713 1653 1341 761
BclI T'GATC_A
Cuts at: 654 1113 2988 654
Size: 459 1875 3134
Fragments arranged by size:
3134 1875 459
BglI GCCn_nnn'nGGC
Cuts at: 1327 1327
Size: 5468
BglII A'GATC_T
Cuts at: 2983 5020 2983
Size: 2037 3431
BlpI GC'TnA_GC
Cuts at: 1846 2580 1846
Size: 734 4734
Bme1580I G_kGCm'C
Cuts at: 135 1063 1094 1583 2059 3093 3186 4242 135
Size: 928 31 489 476 1034 93 1056 1361
Fragments arranged by size:
1361 1056 1034 928 489 476 93 31
BmrI ACTGGGnnnn_n'
Cuts at: 799 1066 1114 2268 2680 2761 3096 5240 799
Size: 267 48 1154 412 81 335 2144 1027
Fragments arranged by size:
2144 1154 1027 412 335 267 81 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3835 3835
Size: 5468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 487 535 1929 448
Size: 39 48 1394 3987
Fragments arranged by size:
3987 1394 48 39
Bpu10I CC'TnA_GC
Cuts at: 1426 2042 1426
Size: 616 4852
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 2644 3523 4015 4313 994
Size: 1650 879 492 298 2149
Fragments arranged by size:
2149 1650 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 358 532 1078 1624 5172 358
Size: 174 546 546 3548 654
Fragments arranged by size:
3548 654 546 546 174
BsaAI yAC'GTr
Cuts at: 2460 2821 2460
Size: 361 5107
BsaBI GATnn'nnATC
Cuts at: 6 659 2982 5019 6
Size: 653 2323 2037 455
Fragments arranged by size:
2323 2037 653 455
BsaHI Gr'CG_yC
Cuts at: 1951 2899 3147 3849 5345 1951
Size: 948 248 702 1496 2074
Fragments arranged by size:
2074 1496 948 702 248
BsaWI w'CCGG_w
Cuts at: 1151 1157 2657 3178 3856 4130 4277 4688 1151
Size: 6 1500 521 678 274 147 411 1931
Fragments arranged by size:
1931 1500 678 521 411 274 147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1275 1305 2186 2216 1275
Size: 30 881 30 4527
Fragments arranged by size:
4527 881 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 280 550 1404 277
Size: 3 270 854 4341
Fragments arranged by size:
4341 854 270 3
BseYI C'CCAG_C
Cuts at: 144 1097 1164 1796 2051 2402 2893 3651 4228
Size: 953 67 632 255 351 491 758 577
Cuts at: 4228 4724 144
Size: 496 888
Fragments arranged by size:
953 888 758 632 577 496 491 351
255 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 759 2200 759
Size: 1441 4027
BsiEI CG_ry'CG
Cuts at: 104 3010 3056 4264 104
Size: 2906 46 1208 1308
Fragments arranged by size:
2906 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 272 1010 1094 2371 2482 3260 3450 4242 272
Size: 738 84 1277 111 778 190 792 1498
Fragments arranged by size:
1498 1277 792 778 738 190 111 84
Bsp1286I G_dGCh'C
Cuts at: 135 272 842 1010 1063 1094 1111 1583 2059
Size: 137 570 168 53 31 17 472 476
Cuts at: 2059 2371 2482 3093 3186 3260 3450 3512 4242
Size: 312 111 611 93 74 190 62 730
Cuts at: 4242 135
Size: 1361
Fragments arranged by size:
1361 730 611 570 476 472 312 190
168 137 111 93 74 62 53 31
17
BspEI T'CCGG_A
Cuts at: 1157 4688 1157
Size: 3531 1937
BspHI T'CATG_A
Cuts at: 1482 4644 1482
Size: 3162 2306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 415 1417 1546 2518 2732 3034 3415 415
Size: 1002 129 972 214 302 381 2468
Fragments arranged by size:
2468 1002 972 381 302 214 129
BsrBI CCG'CTC
Cuts at: 50 303 2046 3760 3814 5298 5463 50
Size: 253 1743 1714 54 1484 165 55
Fragments arranged by size:
1743 1714 1484 253 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 3380 2153
Size: 1227 4241
BsrFI r'CCGG_y
Cuts at: 935 1151 3466 3647 3856 935
Size: 216 2315 181 209 2547
Fragments arranged by size:
2547 2315 216 209 181
BsrGI T'GTAC_A
Cuts at: 485 485
Size: 5468
BssSI C'ACGA_G
Cuts at: 2396 3739 4097 2396
Size: 1343 358 3767
Fragments arranged by size:
3767 1343 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1547 1630 2845 1547
Size: 83 1215 4170
Fragments arranged by size:
4170 1215 83
BstBI TT'CG_AA
Cuts at: 110 3829 110
Size: 3719 1749
BstEII G'GTnAC_C
Cuts at: 2449 2449
Size: 5468
BstYI r'GATC_y
Cuts at: 1 1355 1710 2493 2960 2983 3318 3564 4576
Size: 1354 355 783 467 23 335 246 1012
Cuts at: 4576 4662 4674 5020 1
Size: 86 12 346 449
Fragments arranged by size:
1354 1012 783 467 449 355 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1535 1535
Size: 5468
BtgI C'CryG_G
Cuts at: 1229 2198 1229
Size: 969 4499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3418 3599 3762 3418
Size: 181 163 5124
Fragments arranged by size:
5124 181 163
BtsI GCAGTG_nn'
Cuts at: 253 827 1019 1980 2283 2331 2711 253
Size: 574 192 961 303 48 380 3010
Fragments arranged by size:
3010 961 574 380 303 192 48
ClaI AT'CG_AT
Cuts at: 827 827
Size: 5468
DraI TTT'AAA
Cuts at: 750 1959 2467 750
Size: 1209 508 3751
Fragments arranged by size:
3751 1209 508
DraIII CAC_nnn'GTG
Cuts at: 1336 1336
Size: 5468
DrdI GACnn_nn'nnGTC
Cuts at: 768 2413 3174 4032 768
Size: 1645 761 858 2204
Fragments arranged by size:
2204 1645 858 761
EaeI y'GGCC_r
Cuts at: 321 896 2032 2243 3053 3227 3618 3645 4990
Size: 575 1136 211 810 174 391 27 1345
Cuts at: 4990 321
Size: 799
Fragments arranged by size:
1345 1136 810 799 575 391 211 174
27
EagI C'GGCC_G
Cuts at: 3053 3053
Size: 5468
EarI CTCTTCn'nnn_
Cuts at: 433 518 958 3491 3701 433
Size: 85 440 2533 210 2200
Fragments arranged by size:
2533 2200 440 210 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3676 3986 4132 5278 3676
Size: 310 146 1146 3866
Fragments arranged by size:
3866 1146 310 146
EcoICRI GAG'CTC
Cuts at: 270 1008 2480 270
Size: 738 1472 3258
Fragments arranged by size:
3258 1472 738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 487 535 1009 1199 1329 1345 1795 1929
Size: 39 48 474 190 130 16 450 134
Cuts at: 1929 3293 3725 4472 4740 448
Size: 1364 432 747 268 1176
Fragments arranged by size:
1364 1176 747 474 450 432 268 190
134 130 48 39 16
EcoNI CCTnn'n_nnAGG
Cuts at: 346 1908 346
Size: 1562 3906
EcoO109I rG'GnC_Cy
Cuts at: 156 891 1060 2055 2056 2607 156
Size: 735 169 995 1 551 3017
Fragments arranged by size:
3017 995 735 551 169 1
EcoRI G'AATT_C
Cuts at: 2472 2472
Size: 5468
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 209 3487 209
Size: 3278 2190
FspI TGC'GCA
Cuts at: 3249 3249
Size: 5468
HaeII r_GCGC'y
Cuts at: 288 1803 2902 3150 4172 5151 288
Size: 1515 1099 248 1022 979 605
Fragments arranged by size:
1515 1099 1022 979 605 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 1105 1137 1641 1673 2353 2385 3310
Size: 32 385 32 504 32 680 32 925
Cuts at: 3310 3340 3342 3372 688
Size: 30 2 30 2784
Fragments arranged by size:
2784 925 680 504 385 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2507 2507
Size: 5468
HindIII A'AGCT_T
Cuts at: 980 980
Size: 5468
KasI G'GCGC_C
Cuts at: 2898 3146 2898
Size: 248 5220
KpnI G_GTAC'C
Cuts at: 2488 2488
Size: 5468
MluI A'CGCG_T
Cuts at: 4901 4901
Size: 5468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1419 1787 4139 4323 1419
Size: 368 2352 184 2564
Fragments arranged by size:
2564 2352 368 184
MscI TGG'CCA
Cuts at: 323 898 3229 323
Size: 575 2331 2562
Fragments arranged by size:
2562 2331 575
MslI CAynn'nnrTG
Cuts at: 1517 3457 3584 1517
Size: 1940 127 3401
Fragments arranged by size:
3401 1940 127
MspA1I CmG'CkG
Cuts at: 200 257 1349 1430 1499 2015 2579 2893 3253
Size: 57 1092 81 69 516 564 314 360
Cuts at: 3253 4266 4511 200
Size: 1013 245 1157
Fragments arranged by size:
1157 1092 1013 564 516 360 314 245
81 69 57
NaeI GCC'GGC
Cuts at: 3649 3649
Size: 5468
NarI GG'CG_CC
Cuts at: 2899 3147 2899
Size: 248 5220
NgoMIV G'CCGG_C
Cuts at: 3647 3647
Size: 5468
NspI r_CATG'y
Cuts at: 1750 2521 3021 3928 1750
Size: 771 500 907 3290
Fragments arranged by size:
3290 907 771 500
PciI A'CATG_T
Cuts at: 3924 3924
Size: 5468
PflMI CCAn_nnn'nTGG
Cuts at: 1104 1104
Size: 5468
PfoI T'CCnGG_A
Cuts at: 503 1145 503
Size: 642 4826
PmeI GTTT'AAAC
Cuts at: 2467 2467
Size: 5468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4639 4607
Size: 32 5436
PpuMI rG'GwC_Cy
Cuts at: 891 891
Size: 5468
PspOMI G'GGCC_C
Cuts at: 1059 2055 1059
Size: 996 4472
PstI C_TGCA'G
Cuts at: 241 248 1068 1504 2515 3200 241
Size: 7 820 436 1011 685 2509
Fragments arranged by size:
2509 1011 820 685 436 7
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5468
PvuII CAG'CTG
Cuts at: 1430 2015 2893 3253 1430
Size: 585 878 360 3645
Fragments arranged by size:
3645 878 585 360
RsrII CG'GwC_CG
Cuts at: 3663 3663
Size: 5468
SacI G_AGCT'C
Cuts at: 272 1010 2482 272
Size: 738 1472 3258
Fragments arranged by size:
3258 1472 738
SalI G'TCGA_C
Cuts at: 2505 2505
Size: 5468
SapI GCTCTTCn'nnn_
Cuts at: 3491 3701 3491
Size: 210 5258
SbfI CC_TGCA'GG
Cuts at: 2515 2515
Size: 5468
ScaI AGT'ACT
Cuts at: 1693 4985 1693
Size: 3292 2176
SexAI A'CCwGG_T
Cuts at: 369 369
Size: 5468
SfcI C'TryA_G
Cuts at: 33 237 244 1064 1500 2511 3196 4189 4380
Size: 204 7 820 436 1011 685 993 191
Cuts at: 4380 33
Size: 1121
Fragments arranged by size:
1121 1011 993 820 685 436 204 191
7
SfoI GGC'GCC
Cuts at: 2900 3148 2900
Size: 248 5220
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
SmaI CCC'GGG
Cuts at: 1304 2490 1304
Size: 1186 4282
SmlI C'TyrA_G
Cuts at: 886 1009 2623 3538 4030 4292 886
Size: 123 1614 915 492 262 2062
Fragments arranged by size:
2062 1614 915 492 262 123
SnaBI TAC'GTA
Cuts at: 2460 2460
Size: 5468
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5468
SphI G_CATG'C
Cuts at: 2521 3021 2521
Size: 500 4968
StuI AGG'CCT
Cuts at: 1546 2250 1546
Size: 704 4764
StyI C'CwwG_G
Cuts at: 963 1001 1851 1862 2602 2956 963
Size: 38 850 11 740 354 3475
Fragments arranged by size:
3475 850 740 354 38 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1111 2250 3051 3184 3643 3852 1111
Size: 1139 801 133 459 209 2727
Fragments arranged by size:
2727 1139 801 459 209 133
TatI w'GTAC_w
Cuts at: 485 1691 4983 485
Size: 1206 3292 970
Fragments arranged by size:
3292 1206 970
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1275 1307 2186 2218 4985 5017 1275
Size: 32 879 32 2767 32 1726
Fragments arranged by size:
2767 1726 879 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 910 3265 910
Size: 2355 3113
XbaI T'CTAG_A
Cuts at: 2499 2499
Size: 5468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2963 2963
Size: 5468
XmaI C'CCGG_G
Cuts at: 1302 2488 1302
Size: 1186 4282
XmnI GAAnn'nnTTC
Cuts at: 602 994 2305 602
Size: 392 1311 3765
Fragments arranged by size:
3765 1311 392
ZraI GAC'GTC
Cuts at: 1952 1952
Size: 5468
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AlfI AloI AscI AvrII BcgI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BssHII BstXI
Bsu36I CspCI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI SspI SwaI XhoI