Restriction map for
pF1KSDA1599
(Circular) (Six-base) MAPSORT of: pF1KSDA1599.seq Check: 716 from: 1 to: 4916
pF1KSDA1599 4916 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 274 1954 274
Size: 1680 3236
Acc65I G'GTAC_C
Cuts at: 1932 1932
Size: 4916
AclI AA'CG_TT
Cuts at: 757 4756 757
Size: 3999 917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 3173 3920 4188 2741
Size: 432 747 268 3469
Fragments arranged by size:
3469 747 432 268
AflIII A'CryG_T
Cuts at: 239 2268 3372 4349 239
Size: 2029 1104 977 806
Fragments arranged by size:
2029 1104 977 806
AgeI A'CCGG_T
Cuts at: 3304 3304
Size: 4916
AhdI GACnn_n'nnGTC
Cuts at: 147 147
Size: 4916
AleI CACnn'nnGTG
Cuts at: 196 1444 196
Size: 1248 3668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 586 692 1747 586
Size: 106 1055 3755
Fragments arranged by size:
3755 1055 106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 742 774 742
Size: 32 4884
AlwNI CAG_nnn'CTG
Cuts at: 647 2221 3788 647
Size: 1574 1567 1775
Fragments arranged by size:
1775 1574 1567
ApaLI G'TGCA_C
Cuts at: 1068 3686 1068
Size: 2618 2298
ApoI r'AATT_y
Cuts at: 549 1023 1920 4269 4525 549
Size: 474 897 2349 256 940
Fragments arranged by size:
2349 940 897 474 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4916
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
AvaI C'yCGr_G
Cuts at: 1655 1754 1936 1655
Size: 99 182 4635
Fragments arranged by size:
4635 182 99
BamHI G'GATC_C
Cuts at: 1941 1941
Size: 4916
BanI G'GyrC_C
Cuts at: 613 1932 2346 2594 2629 613
Size: 1319 414 248 35 2900
Fragments arranged by size:
2900 1319 414 248 35
BanII G_rGCy'C
Cuts at: 641 840 1183 1930 2960 641
Size: 199 343 747 1030 2597
Fragments arranged by size:
2597 1030 747 343 199
BbeI G_GCGC'C
Cuts at: 2350 2598 2350
Size: 248 4668
BbvCI CC'TCA_GC
Cuts at: 632 632
Size: 4916
BciVI GTATCCnnnnn_n'
Cuts at: 2814 3575 4916 2814
Size: 761 1341 2814
Fragments arranged by size:
2814 1341 761
BclI T'GATC_A
Cuts at: 2436 2436
Size: 4916
BglI GCCn_nnn'nGGC
Cuts at: 1750 1750
Size: 4916
BglII A'GATC_T
Cuts at: 2431 4468 2431
Size: 2037 2879
BlpI GC'TnA_GC
Cuts at: 154 640 1735 2028 154
Size: 486 1095 293 3042
Fragments arranged by size:
3042 1095 486 293
Bme1580I G_kGCm'C
Cuts at: 1072 2541 2634 3690 1072
Size: 1469 93 1056 2298
Fragments arranged by size:
2298 1469 1056 93
BmgBI CAC'GTC
Cuts at: 1641 1761 1641
Size: 120 4796
BmrI ACTGGGnnnn_n'
Cuts at: 897 1476 2128 2209 2544 4688 897
Size: 579 652 81 335 2144 1125
Fragments arranged by size:
2144 1125 652 579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 183 1362 3283 183
Size: 1179 1921 1816
Fragments arranged by size:
1921 1816 1179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 1393
Size: 4916
Bpu10I CC'TnA_GC
Cuts at: 632 632
Size: 4916
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2092 2971 3463 3761 2092
Size: 879 492 298 3247
Fragments arranged by size:
3247 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4620 4620
Size: 4916
BsaAI yAC'GTr
Cuts at: 1020 1446 1710 1731 1908 2269 1020
Size: 426 264 21 177 361 3667
Fragments arranged by size:
3667 426 361 264 177 21
BsaBI GATnn'nnATC
Cuts at: 6 2430 4467 6
Size: 2424 2037 455
Fragments arranged by size:
2424 2037 455
BsaHI Gr'CG_yC
Cuts at: 148 1613 2347 2595 3297 4793 148
Size: 1465 734 248 702 1496 271
Fragments arranged by size:
1496 1465 734 702 271 248
BsaWI w'CCGG_w
Cuts at: 2105 2626 3304 3578 3725 4136 2105
Size: 521 678 274 147 411 2885
Fragments arranged by size:
2885 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 476 506 1051 1081 476
Size: 30 545 30 4311
Fragments arranged by size:
4311 545 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 649 1641 400
Size: 249 992 3675
Fragments arranged by size:
3675 992 249
BseYI C'CCAG_C
Cuts at: 1863 2341 3099 3676 4172 1863
Size: 478 758 577 496 2607
Fragments arranged by size:
2607 758 577 496 478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 1153 1423 1489 1876 459
Size: 694 270 66 387 3499
Fragments arranged by size:
3499 694 387 270 66
BsiEI CG_ry'CG
Cuts at: 104 861 1466 2458 2504 3712 104
Size: 757 605 992 46 1208 1308
Fragments arranged by size:
1308 1208 992 757 605 46
BsiHKAI G_wGCw'C
Cuts at: 641 1072 1930 2708 2898 3690 641
Size: 431 858 778 190 792 1867
Fragments arranged by size:
1867 858 792 778 431 190
BsmI GAATG_Cn'
Cuts at: 538 1461 1811 538
Size: 923 350 3643
Fragments arranged by size:
3643 923 350
BsmBI CGTCTCn'nnnn_
Cuts at: 1751 1751
Size: 4916
Bsp1286I G_dGCh'C
Cuts at: 641 840 1072 1183 1930 2541 2634 2708 2898
Size: 199 232 111 747 611 93 74 190
Cuts at: 2898 2960 3690 641
Size: 62 730 1867
Fragments arranged by size:
1867 747 730 611 232 199 190 111
93 74 62
BspEI T'CCGG_A
Cuts at: 4136 4136
Size: 4916
BspHI T'CATG_A
Cuts at: 785 4092 785
Size: 3307 1609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1966 2180 2482 2863 1966
Size: 214 302 381 4019
Fragments arranged by size:
4019 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3208 3262 4746 4911 50
Size: 3158 54 1484 165 55
Fragments arranged by size:
3158 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2828 2828
Size: 4916
BsrFI r'CCGG_y
Cuts at: 2914 3095 3304 2914
Size: 181 209 4526
Fragments arranged by size:
4526 209 181
BssSI C'ACGA_G
Cuts at: 1312 3187 3545 1312
Size: 1875 358 2683
Fragments arranged by size:
2683 1875 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2293 2293
Size: 4916
BstBI TT'CG_AA
Cuts at: 110 3277 110
Size: 3167 1749
BstEII G'GTnAC_C
Cuts at: 1437 1437
Size: 4916
BstXI CCAn_nnnn'nTGG
Cuts at: 198 687 873 198
Size: 489 186 4241
Fragments arranged by size:
4241 489 186
BstYI r'GATC_y
Cuts at: 1 1646 1772 1941 2408 2431 2766 3012 4024
Size: 1645 126 169 467 23 335 246 1012
Cuts at: 4024 4110 4122 4468 1
Size: 86 12 346 449
Fragments arranged by size:
1645 1012 467 449 346 335 246 169
126 86 23 12
BstZ17I GTA'TAC
Cuts at: 275 275
Size: 4916
Bsu36I CC'TnA_GG
Cuts at: 137 137
Size: 4916
BtgI C'CryG_G
Cuts at: 125 951 1433 1616 125
Size: 826 482 183 3425
Fragments arranged by size:
3425 826 482 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2866 3047 3210 2866
Size: 181 163 4572
Fragments arranged by size:
4572 181 163
BtsI GCAGTG_nn'
Cuts at: 304 1130 2159 304
Size: 826 1029 3061
Fragments arranged by size:
3061 1029 826
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1407 1442 1407
Size: 35 4881
DraI TTT'AAA
Cuts at: 1915 1915
Size: 4916
DrdI GACnn_nn'nnGTC
Cuts at: 2622 3480 2622
Size: 858 4058
EaeI y'GGCC_r
Cuts at: 188 1118 1448 1463 2501 2675 3066 3093 4438
Size: 930 330 15 1038 174 391 27 1345
Cuts at: 4438 188
Size: 666
Fragments arranged by size:
1345 1038 930 666 391 330 174 27
15
EagI C'GGCC_G
Cuts at: 1463 2501 1463
Size: 1038 3878
EarI CTCTTCn'nnn_
Cuts at: 2939 3149 2939
Size: 210 4706
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3124 3434 3580 4726 3124
Size: 310 146 1146 3314
Fragments arranged by size:
3314 1146 310 146
EcoICRI GAG'CTC
Cuts at: 639 1928 639
Size: 1289 3627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 2741 3173 3920 4188 1393
Size: 1348 432 747 268 2121
Fragments arranged by size:
2121 1348 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 4916
EcoO109I rG'GnC_Cy
Cuts at: 497 1097 2055 497
Size: 600 958 3358
Fragments arranged by size:
3358 958 600
EcoRI G'AATT_C
Cuts at: 549 1023 1920 549
Size: 474 897 3545
Fragments arranged by size:
3545 897 474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 4916
FspI TGC'GCA
Cuts at: 358 1396 2697 358
Size: 1038 1301 2577
Fragments arranged by size:
2577 1301 1038
HaeII r_GCGC'y
Cuts at: 1213 2350 2598 3620 4599 1213
Size: 1137 248 1022 979 1530
Fragments arranged by size:
1530 1137 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 409 441 1217 1249 2758 2788 2790
Size: 32 244 32 776 32 1509 30 2
Cuts at: 2790 2820 133
Size: 30 2229
Fragments arranged by size:
2229 1509 776 244 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 423 1554 1955 423
Size: 1131 401 3384
Fragments arranged by size:
3384 1131 401
KasI G'GCGC_C
Cuts at: 2346 2594 2346
Size: 248 4668
KpnI G_GTAC'C
Cuts at: 1936 1936
Size: 4916
MluI A'CGCG_T
Cuts at: 4349 4349
Size: 4916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 278 608 1203 3587 3771 278
Size: 330 595 2384 184 1423
Fragments arranged by size:
2384 1423 595 330 184
MscI TGG'CCA
Cuts at: 1120 1450 2677 1120
Size: 330 1227 3359
Fragments arranged by size:
3359 1227 330
MslI CAynn'nnrTG
Cuts at: 196 829 871 1444 2905 3032 196
Size: 633 42 573 1461 127 2080
Fragments arranged by size:
2080 1461 633 573 127 42
MspA1I CmG'CkG
Cuts at: 577 631 1191 1407 1462 2027 2341 2701 3714
Size: 54 560 216 55 565 314 360 1013
Cuts at: 3714 3959 577
Size: 245 1534
Fragments arranged by size:
1534 1013 565 560 360 314 245 216
55 54
NaeI GCC'GGC
Cuts at: 3097 3097
Size: 4916
NarI GG'CG_CC
Cuts at: 2347 2595 2347
Size: 248 4668
NcoI C'CATG_G
Cuts at: 125 1616 125
Size: 1491 3425
NgoMIV G'CCGG_C
Cuts at: 3095 3095
Size: 4916
NspI r_CATG'y
Cuts at: 243 1969 2469 3376 243
Size: 1726 500 907 1783
Fragments arranged by size:
1783 1726 907 500
PasI CC'CwG_GG
Cuts at: 502 1269 502
Size: 767 4149
PciI A'CATG_T
Cuts at: 239 3372 239
Size: 3133 1783
PflMI CCAn_nnn'nTGG
Cuts at: 285 1327 285
Size: 1042 3874
PfoI T'CCnGG_A
Cuts at: 227 553 1370 1700 227
Size: 326 817 330 3443
Fragments arranged by size:
3443 817 330 326
PmeI GTTT'AAAC
Cuts at: 1915 1915
Size: 4916
PmlI CAC'GTG
Cuts at: 1446 1731 1446
Size: 285 4631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 4055
Size: 32 4884
PpuMI rG'GwC_Cy
Cuts at: 497 1097 497
Size: 600 4316
PshAI GACnn'nnGTC
Cuts at: 781 1219 781
Size: 438 4478
PstI C_TGCA'G
Cuts at: 220 1963 2648 220
Size: 1743 685 2488
Fragments arranged by size:
2488 1743 685
PvuI CG_AT'CG
Cuts at: 104 861 104
Size: 757 4159
PvuII CAG'CTG
Cuts at: 577 1191 1407 2341 2701 577
Size: 614 216 934 360 2792
Fragments arranged by size:
2792 934 614 360 216
RsrII CG'GwC_CG
Cuts at: 3111 3111
Size: 4916
SacI G_AGCT'C
Cuts at: 641 1930 641
Size: 1289 3627
SalI G'TCGA_C
Cuts at: 1953 1953
Size: 4916
SanDI GG'GwC_CC
Cuts at: 497 1097 497
Size: 600 4316
SapI GCTCTTCn'nnn_
Cuts at: 2939 3149 2939
Size: 210 4706
SbfI CC_TGCA'GG
Cuts at: 220 1963 220
Size: 1743 3173
ScaI AGT'ACT
Cuts at: 1486 4433 1486
Size: 2947 1969
SfcI C'TryA_G
Cuts at: 33 216 648 735 1959 2644 3637 3828 33
Size: 183 432 87 1224 685 993 191 1121
Fragments arranged by size:
1224 1121 993 685 432 191 183 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 1750 1750
Size: 4916
SfoI GGC'GCC
Cuts at: 2348 2596 2348
Size: 248 4668
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
SmaI CCC'GGG
Cuts at: 1657 1938 1657
Size: 281 4635
SmlI C'TyrA_G
Cuts at: 2071 2986 3478 3740 2071
Size: 915 492 262 3247
Fragments arranged by size:
3247 915 492 262
SnaBI TAC'GTA
Cuts at: 1908 1908
Size: 4916
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4916
SphI G_CATG'C
Cuts at: 1969 2469 1969
Size: 500 4416
StuI AGG'CCT
Cuts at: 469 1378 469
Size: 909 4007
StyI C'CwwG_G
Cuts at: 125 170 194 279 480 1538 1616 2050 2404
Size: 45 24 85 201 1058 78 434 354
Cuts at: 2404 125
Size: 2637
Fragments arranged by size:
2637 1058 434 354 201 85 78 45
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 793 1456 2499 2632 3091 3300 793
Size: 663 1043 133 459 209 2409
Fragments arranged by size:
2409 1043 663 459 209 133
TatI w'GTAC_w
Cuts at: 1484 4431 1484
Size: 2947 1969
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 327 359 476 508 786 818 1051 1083 4433
Size: 32 117 32 278 32 233 32 3350
Cuts at: 4433 4465 327
Size: 32 778
Fragments arranged by size:
3350 778 278 233 117 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1681 2713 1681
Size: 1032 3884
XbaI T'CTAG_A
Cuts at: 1947 1947
Size: 4916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 913 1623 2411 913
Size: 710 788 3418
Fragments arranged by size:
3418 788 710
XmaI C'CCGG_G
Cuts at: 1655 1936 1655
Size: 281 4635
XmnI GAAnn'nnTTC
Cuts at: 700 700
Size: 4916
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII ApaI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BsrGI BssHII ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PsiI PspOMI PspXI
PsrI SacII SexAI SgrAI SrfI SspI SwaI XhoI
ZraI