Restriction map for
pF1KSDA1605
(Circular) (Six-base) MAPSORT of: pF1KSDA1605.seq Check: 5290 from: 1 to: 5768
pF1KSDA1605 5768 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 880 1197 1458 880
Size: 317 261 5190
Fragments arranged by size:
5190 317 261
AccI GT'mk_AC
Cuts at: 1414 2600 2806 1414
Size: 1186 206 4376
Fragments arranged by size:
4376 1186 206
Acc65I G'GTAC_C
Cuts at: 485 500 1850 2784 485
Size: 15 1350 934 3469
Fragments arranged by size:
3469 1350 934 15
AclI AA'CG_TT
Cuts at: 5608 5608
Size: 5768
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 1210 2011 2068 2425 2624 2725 3593 4025
Size: 971 801 57 357 199 101 868 432
Cuts at: 4025 4772 5040 239
Size: 747 268 967
Fragments arranged by size:
971 967 868 801 747 432 357 268
199 101 57
AflIII A'CryG_T
Cuts at: 1652 2054 2454 3120 4224 5201 1652
Size: 402 400 666 1104 977 2219
Fragments arranged by size:
2219 1104 977 666 402 400
AgeI A'CCGG_T
Cuts at: 4156 4156
Size: 5768
AleI CACnn'nnGTG
Cuts at: 2437 2437
Size: 5768
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1565 1597 1919 1951 1565
Size: 32 322 32 5382
Fragments arranged by size:
5382 322 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1450 3073 4640 1450
Size: 1623 1567 2578
Fragments arranged by size:
2578 1623 1567
ApaLI G'TGCA_C
Cuts at: 4538 4538
Size: 5768
ApoI r'AATT_y
Cuts at: 2772 5121 5377 2772
Size: 2349 256 3163
Fragments arranged by size:
3163 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5768
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5768
AvaI C'yCGr_G
Cuts at: 824 1325 2788 824
Size: 501 1463 3804
Fragments arranged by size:
3804 1463 501
AvrII C'CTAG_G
Cuts at: 2418 2418
Size: 5768
BamHI G'GATC_C
Cuts at: 138 198 2793 138
Size: 60 2595 3113
Fragments arranged by size:
3113 2595 60
BanI G'GyrC_C
Cuts at: 232 366 485 500 1850 1872 2748 2784 3198
Size: 134 119 15 1350 22 876 36 414
Cuts at: 3198 3446 3481 232
Size: 248 35 2519
Fragments arranged by size:
2519 1350 876 414 248 134 119 36
35 22 15
BanII G_rGCy'C
Cuts at: 1052 1519 1793 2633 2690 2782 3812 1052
Size: 467 274 840 57 92 1030 3008
Fragments arranged by size:
3008 1030 840 467 274 92 57
BbeI G_GCGC'C
Cuts at: 3202 3450 3202
Size: 248 5520
BbsI GAAGACnn'nnnn_
Cuts at: 278 514 532 278
Size: 236 18 5514
Fragments arranged by size:
5514 236 18
BbvCI CC'TCA_GC
Cuts at: 1456 2281 1456
Size: 825 4943
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1316 1350 1993 2027 1316
Size: 34 643 34 5057
Fragments arranged by size:
5057 643 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3666 4427 5768 3666
Size: 761 1341 3666
Fragments arranged by size:
3666 1341 761
BclI T'GATC_A
Cuts at: 545 2034 3288 545
Size: 1489 1254 3025
Fragments arranged by size:
3025 1489 1254
BfrBI ATG'CAT
Cuts at: 1948 1948
Size: 5768
BglI GCCn_nnn'nGGC
Cuts at: 2521 2521
Size: 5768
BglII A'GATC_T
Cuts at: 3283 5320 3283
Size: 2037 3731
BlpI GC'TnA_GC
Cuts at: 1792 2880 1792
Size: 1088 4680
Bme1580I G_kGCm'C
Cuts at: 2250 3393 3486 4542 2250
Size: 1143 93 1056 3476
Fragments arranged by size:
3476 1143 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 789 1018 2171 2980 3061 3396 5540 789
Size: 229 1153 809 81 335 2144 1017
Fragments arranged by size:
2144 1153 1017 809 335 229 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1518 4135 1518
Size: 2617 3151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 769 1081 1313 1363 642
Size: 127 312 232 50 5047
Fragments arranged by size:
5047 312 232 127 50
Bpu10I CC'TnA_GC
Cuts at: 448 1456 2281 448
Size: 1008 825 3935
Fragments arranged by size:
3935 1008 825
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 1639 1723 1807 2944 3823 4315 4613 501
Size: 1138 84 84 1137 879 492 298 1656
Fragments arranged by size:
1656 1138 1137 879 492 298 84 84
BsaI GGTCTCn'nnnn_
Cuts at: 5472 5472
Size: 5768
BsaAI yAC'GTr
Cuts at: 2760 3121 2760
Size: 361 5407
BsaBI GATnn'nnATC
Cuts at: 6 3282 5319 6
Size: 3276 2037 455
Fragments arranged by size:
3276 2037 455
BsaHI Gr'CG_yC
Cuts at: 161 3199 3447 4149 5645 161
Size: 3038 248 702 1496 284
Fragments arranged by size:
3038 1496 702 284 248
BsaWI w'CCGG_w
Cuts at: 309 503 671 2957 3478 4156 4430 4577 4988
Size: 194 168 2286 521 678 274 147 411
Cuts at: 4988 309
Size: 1089
Fragments arranged by size:
2286 1089 678 521 411 274 194 168
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 427 457 923 953 1586 1616 1919 1949 427
Size: 30 466 30 633 30 303 30 4246
Fragments arranged by size:
4246 633 466 303 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1651 2661 1651
Size: 1010 4758
BseYI C'CCAG_C
Cuts at: 165 1641 2681 3193 3951 4528 5024 165
Size: 1476 1040 512 758 577 496 909
Fragments arranged by size:
1476 1040 909 758 577 512 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1124 2590 265
Size: 859 1466 3443
Fragments arranged by size:
3443 1466 859
BsiEI CG_ry'CG
Cuts at: 104 1689 3310 3356 4564 104
Size: 1585 1621 46 1208 1308
Fragments arranged by size:
1621 1585 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1793 2469 2633 2690 2782 3560 3750 4542 1793
Size: 676 164 57 92 778 190 792 3019
Fragments arranged by size:
3019 792 778 676 190 164 92 57
BsmBI CGTCTCn'nnnn_
Cuts at: 286 447 1013 286
Size: 161 566 5041
Fragments arranged by size:
5041 566 161
Bsp1286I G_dGCh'C
Cuts at: 1052 1519 1793 2250 2469 2633 2690 2782 3393
Size: 467 274 457 219 164 57 92 611
Cuts at: 3393 3486 3560 3750 3812 4542 1052
Size: 93 74 190 62 730 2278
Fragments arranged by size:
2278 730 611 467 457 274 219 190
164 93 92 74 62 57
BspEI T'CCGG_A
Cuts at: 309 4988 309
Size: 4679 1089
BspHI T'CATG_A
Cuts at: 399 4944 399
Size: 4545 1223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 237 497 880 1197 1458 1995 2818 3032 3334
Size: 260 383 317 261 537 823 214 302
Cuts at: 3334 3715 237
Size: 381 2290
Fragments arranged by size:
2290 823 537 383 381 317 302 261
260 214
BsrBI CCG'CTC
Cuts at: 50 4060 4114 5598 5763 50
Size: 4010 54 1484 165 55
Fragments arranged by size:
4010 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1286 3680 1286
Size: 2394 3374
BsrFI r'CCGG_y
Cuts at: 157 3766 3947 4156 157
Size: 3609 181 209 1769
Fragments arranged by size:
3609 1769 209 181
BsrGI T'GTAC_A
Cuts at: 1724 1724
Size: 5768
BssSI C'ACGA_G
Cuts at: 1142 4039 4397 1142
Size: 2897 358 2513
Fragments arranged by size:
2897 2513 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1450 3145 1450
Size: 1695 4073
BstBI TT'CG_AA
Cuts at: 110 4129 110
Size: 4019 1749
BstEII G'GTnAC_C
Cuts at: 1164 2163 1164
Size: 999 4769
BstXI CCAn_nnnn'nTGG
Cuts at: 1909 2389 1909
Size: 480 5288
BstYI r'GATC_y
Cuts at: 1 138 198 1218 1499 2793 3260 3283 3618
Size: 137 60 1020 281 1294 467 23 335
Cuts at: 3618 3864 4876 4962 4974 5320 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1294 1020 1012 467 449 346 335 281
246 137 86 60 23 12
BstZ17I GTA'TAC
Cuts at: 1415 1415
Size: 5768
BtgI C'CryG_G
Cuts at: 125 615 1160 1930 2516 2544 125
Size: 490 545 770 586 28 3349
Fragments arranged by size:
3349 770 586 545 490 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 666 3718 3899 4062 666
Size: 3052 181 163 2372
Fragments arranged by size:
3052 2372 181 163
BtsI GCAGTG_nn'
Cuts at: 499 1537 3011 499
Size: 1038 1474 3256
Fragments arranged by size:
3256 1474 1038
DraI TTT'AAA
Cuts at: 2767 2767
Size: 5768
DraIII CAC_nnn'GTG
Cuts at: 701 1604 701
Size: 903 4865
DrdI GACnn_nn'nnGTC
Cuts at: 3474 4332 3474
Size: 858 4910
EaeI y'GGCC_r
Cuts at: 852 1254 1428 1817 2322 3353 3527 3918 3945
Size: 402 174 389 505 1031 174 391 27
Cuts at: 3945 5290 852
Size: 1345 1330
Fragments arranged by size:
1345 1330 1031 505 402 391 389 174
174 27
EagI C'GGCC_G
Cuts at: 3353 3353
Size: 5768
EarI CTCTTCn'nnn_
Cuts at: 215 409 1487 2744 3791 4001 215
Size: 194 1078 1257 1047 210 1982
Fragments arranged by size:
1982 1257 1078 1047 210 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1654 3976 4286 4432 5578 1654
Size: 2322 310 146 1146 1844
Fragments arranged by size:
2322 1844 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1791 2631 2688 2780 1791
Size: 840 57 92 4779
Fragments arranged by size:
4779 840 92 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 642 769 1081 1210 1313 1363 2011 2068
Size: 403 127 312 129 103 50 648 57
Cuts at: 2068 2425 2624 2725 3593 4025 4772 5040 239
Size: 357 199 101 868 432 747 268 967
Fragments arranged by size:
967 868 747 648 432 403 357 312
268 199 129 127 103 101 57 50
EcoNI CCTnn'n_nnAGG
Cuts at: 1633 1633
Size: 5768
EcoO109I rG'GnC_Cy
Cuts at: 131 783 1833 1914 1980 2173 2907 131
Size: 652 1050 81 66 193 734 2992
Fragments arranged by size:
2992 1050 734 652 193 81 66
EcoRI G'AATT_C
Cuts at: 2772 2772
Size: 5768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2755 3787 2755
Size: 1032 4736
FspI TGC'GCA
Cuts at: 3549 3549
Size: 5768
HaeII r_GCGC'y
Cuts at: 756 3202 3450 4472 5451 756
Size: 2446 248 1022 979 1073
Fragments arranged by size:
2446 1073 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 123 155 825 857 1519 1551 2220 2252 2332
Size: 32 670 32 662 32 669 32 80
Cuts at: 2332 2364 3610 3640 3642 3672 123
Size: 32 1246 30 2 30 2219
Fragments arranged by size:
2219 1246 670 669 662 80 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2807 2807
Size: 5768
KasI G'GCGC_C
Cuts at: 3198 3446 3198
Size: 248 5520
KpnI G_GTAC'C
Cuts at: 489 504 1854 2788 489
Size: 15 1350 934 3469
Fragments arranged by size:
3469 1350 934 15
MluI A'CGCG_T
Cuts at: 5201 5201
Size: 5768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1129 4439 4623 1129
Size: 3310 184 2274
Fragments arranged by size:
3310 2274 184
MscI TGG'CCA
Cuts at: 854 1256 1430 1819 3529 854
Size: 402 174 389 1710 3093
Fragments arranged by size:
3093 1710 402 389 174
MslI CAynn'nnrTG
Cuts at: 541 1748 2437 3757 3884 541
Size: 1207 689 1320 127 2425
Fragments arranged by size:
2425 1320 1207 689 127
MspA1I CmG'CkG
Cuts at: 327 773 1239 2209 2879 3193 3553 4566 4811
Size: 446 466 970 670 314 360 1013 245
Cuts at: 4811 327
Size: 1284
Fragments arranged by size:
1284 1013 970 670 466 446 360 314
245
NaeI GCC'GGC
Cuts at: 3949 3949
Size: 5768
NarI GG'CG_CC
Cuts at: 3199 3447 3199
Size: 248 5520
NcoI C'CATG_G
Cuts at: 125 1930 2516 2544 125
Size: 1805 586 28 3349
Fragments arranged by size:
3349 1805 586 28
NdeI CA'TA_TG
Cuts at: 1734 1734
Size: 5768
NgoMIV G'CCGG_C
Cuts at: 3947 3947
Size: 5768
NsiI A_TGCA'T
Cuts at: 1950 1950
Size: 5768
NspI r_CATG'y
Cuts at: 1368 1656 1725 2058 2458 2821 3321 4228 1368
Size: 288 69 333 400 363 500 907 2908
Fragments arranged by size:
2908 907 500 400 363 333 288 69
PasI CC'CwG_GG
Cuts at: 1030 1030
Size: 5768
PciI A'CATG_T
Cuts at: 1652 2054 2454 4224 1652
Size: 402 400 1770 3196
Fragments arranged by size:
3196 1770 402 400
PflMI CCAn_nnn'nTGG
Cuts at: 2457 2681 2457
Size: 224 5544
PfoI T'CCnGG_A
Cuts at: 2258 2258
Size: 5768
PmeI GTTT'AAAC
Cuts at: 2767 2767
Size: 5768
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1919 1951 4907 4939 1919
Size: 32 2956 32 2748
Fragments arranged by size:
2956 2748 32 32
PpuMI rG'GwC_Cy
Cuts at: 131 1833 1914 1980 131
Size: 1702 81 66 3919
Fragments arranged by size:
3919 1702 81 66
PshAI GACnn'nnGTC
Cuts at: 1685 1685
Size: 5768
PspXI vC'TCGA_Gb
Cuts at: 1325 1325
Size: 5768
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1405 1437 1405
Size: 32 5736
PstI C_TGCA'G
Cuts at: 494 2006 2815 3500 494
Size: 1512 809 685 2762
Fragments arranged by size:
2762 1512 809 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5768
PvuII CAG'CTG
Cuts at: 327 773 1239 2209 3193 3553 327
Size: 446 466 970 984 360 2542
Fragments arranged by size:
2542 984 970 466 446 360
RsrII CG'GwC_CG
Cuts at: 3963 3963
Size: 5768
SacI G_AGCT'C
Cuts at: 1793 2633 2690 2782 1793
Size: 840 57 92 4779
Fragments arranged by size:
4779 840 92 57
SalI G'TCGA_C
Cuts at: 2805 2805
Size: 5768
SanDI GG'GwC_CC
Cuts at: 131 1914 131
Size: 1783 3985
SapI GCTCTTCn'nnn_
Cuts at: 2744 3791 4001 2744
Size: 1047 210 4511
Fragments arranged by size:
4511 1047 210
SbfI CC_TGCA'GG
Cuts at: 2815 2815
Size: 5768
ScaI AGT'ACT
Cuts at: 5285 5285
Size: 5768
SexAI A'CCwGG_T
Cuts at: 362 362
Size: 5768
SfcI C'TryA_G
Cuts at: 33 490 926 1404 1798 2002 2811 3496 4489
Size: 457 436 478 394 204 809 685 993
Cuts at: 4489 4680 33
Size: 191 1121
Fragments arranged by size:
1121 993 809 685 478 457 436 394
204 191
SfoI GGC'GCC
Cuts at: 3200 3448 3200
Size: 248 5520
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5768
SmaI CCC'GGG
Cuts at: 2790 2790
Size: 5768
SmlI C'TyrA_G
Cuts at: 480 1325 1618 1702 1786 2923 3838 4330 4592
Size: 845 293 84 84 1137 915 492 262
Cuts at: 4592 480
Size: 1656
Fragments arranged by size:
1656 1137 915 845 492 293 262 84
84
SnaBI TAC'GTA
Cuts at: 2760 2760
Size: 5768
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5768
SphI G_CATG'C
Cuts at: 2821 3321 2821
Size: 500 5268
StuI AGG'CCT
Cuts at: 2410 2500 2410
Size: 90 5678
StyI C'CwwG_G
Cuts at: 125 236 584 1930 2418 2516 2544 2902 3256
Size: 111 348 1346 488 98 28 358 354
Cuts at: 3256 125
Size: 2637
Fragments arranged by size:
2637 1346 488 358 354 348 111 98
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 577 903 1000 2151 2577 2581 3351 3484 3943
Size: 326 97 1151 426 4 770 133 459
Cuts at: 3943 4152 577
Size: 209 2193
Fragments arranged by size:
2193 1151 770 459 426 326 209 133
97 4
TatI w'GTAC_w
Cuts at: 1724 5283 1724
Size: 3559 2209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 923 955 1586 1618 2240 2272 5285 5317 923
Size: 32 631 32 622 32 3013 32 1374
Fragments arranged by size:
3013 1374 631 622 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3565 3565
Size: 5768
XbaI T'CTAG_A
Cuts at: 972 2799 972
Size: 1827 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1037 2152 2724 3263 1037
Size: 1115 572 539 3542
Fragments arranged by size:
3542 1115 572 539
XhoI C'TCGA_G
Cuts at: 1325 1325
Size: 5768
XmaI C'CCGG_G
Cuts at: 2788 2788
Size: 5768
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI BaeI
BmgBI BmtI BsiWI BsmI BssHII Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI PacI PmlI PsiI PspOMI SacII SfiI SgrAI
SrfI SspI SwaI XmnI ZraI