Restriction map for
pF1KSDA1609
(Circular) (Six-base) MAPSORT of: pF1KSDA1609.seq Check: 6297 from: 1 to: 4505
pF1KSDA1609 4505 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 603 603
Size: 4505
AccI GT'mk_AC
Cuts at: 344 1543 344
Size: 1199 3306
Acc65I G'GTAC_C
Cuts at: 1521 1521
Size: 4505
AclI AA'CG_TT
Cuts at: 4345 4345
Size: 4505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 2330 2762 3509 3777 286
Size: 2044 432 747 268 1014
Fragments arranged by size:
2044 1014 747 432 268
AflIII A'CryG_T
Cuts at: 380 1160 1857 2961 3938 380
Size: 780 697 1104 977 947
Fragments arranged by size:
1104 977 947 780 697
AgeI A'CCGG_T
Cuts at: 2893 2893
Size: 4505
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 147 179 709 741 147
Size: 32 530 32 3911
Fragments arranged by size:
3911 530 32 32
AlwNI CAG_nnn'CTG
Cuts at: 564 618 684 1431 1810 3377 564
Size: 54 66 747 379 1567 1692
Fragments arranged by size:
1692 1567 747 379 66 54
ApaI G_GGCC'C
Cuts at: 599 677 998 599
Size: 78 321 4106
Fragments arranged by size:
4106 321 78
ApaLI G'TGCA_C
Cuts at: 538 1284 3275 538
Size: 746 1991 1768
Fragments arranged by size:
1991 1768 746
ApoI r'AATT_y
Cuts at: 1509 3858 4114 1509
Size: 2349 256 1900
Fragments arranged by size:
2349 1900 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
AvaI C'yCGr_G
Cuts at: 605 902 1012 1525 605
Size: 297 110 513 3585
Fragments arranged by size:
3585 513 297 110
BamHI G'GATC_C
Cuts at: 1530 1530
Size: 4505
BanI G'GyrC_C
Cuts at: 914 923 1521 1935 2183 2218 914
Size: 9 598 414 248 35 3201
Fragments arranged by size:
3201 598 414 248 35 9
BanII G_rGCy'C
Cuts at: 599 677 947 998 1472 1519 2549 599
Size: 78 270 51 474 47 1030 2555
Fragments arranged by size:
2555 1030 474 270 78 51 47
BbeI G_GCGC'C
Cuts at: 927 1939 2187 927
Size: 1012 248 3245
Fragments arranged by size:
3245 1012 248
BciVI GTATCCnnnnn_n'
Cuts at: 2403 3164 4505 2403
Size: 761 1341 2403
Fragments arranged by size:
2403 1341 761
BclI T'GATC_A
Cuts at: 198 2025 198
Size: 1827 2678
BglI GCCn_nnn'nGGC
Cuts at: 681 681
Size: 4505
BglII A'GATC_T
Cuts at: 2020 4057 2020
Size: 2037 2468
BlpI GC'TnA_GC
Cuts at: 547 626 1617 547
Size: 79 991 3435
Fragments arranged by size:
3435 991 79
Bme1580I G_kGCm'C
Cuts at: 542 599 677 998 1288 2130 2223 3279 542
Size: 57 78 321 290 842 93 1056 1768
Fragments arranged by size:
1768 1056 842 321 290 93 78 57
BmgBI CAC'GTC
Cuts at: 285 285
Size: 4505
BmrI ACTGGGnnnn_n'
Cuts at: 365 587 672 729 1210 1717 1798 2133 4277
Size: 222 85 57 481 507 81 335 2144
Cuts at: 4277 365
Size: 593
Fragments arranged by size:
2144 593 507 481 335 222 85 81
57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 4505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 1456
Size: 4505
Bpu10I CC'TnA_GC
Cuts at: 821 1316 821
Size: 495 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1681 2560 3052 3350 1681
Size: 879 492 298 2836
Fragments arranged by size:
2836 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1355 4209 1355
Size: 2854 1651
BsaAI yAC'GTr
Cuts at: 543 1283 1292 1497 1858 543
Size: 740 9 205 361 3190
Fragments arranged by size:
3190 740 361 205 9
BsaBI GATnn'nnATC
Cuts at: 6 2019 4056 6
Size: 2013 2037 455
Fragments arranged by size:
2037 2013 455
BsaHI Gr'CG_yC
Cuts at: 924 1936 2184 2886 4382 924
Size: 1012 248 702 1496 1047
Fragments arranged by size:
1496 1047 1012 702 248
BsaWI w'CCGG_w
Cuts at: 1694 2215 2893 3167 3314 3725 1694
Size: 521 678 274 147 411 2474
Fragments arranged by size:
2474 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 147 177 946 976 147
Size: 30 769 30 3676
Fragments arranged by size:
3676 769 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 1387 451
Size: 936 3569
BseYI C'CCAG_C
Cuts at: 893 965 1117 1930 2688 3265 3761 893
Size: 72 152 813 758 577 496 1637
Fragments arranged by size:
1637 813 758 577 496 152 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 631
Size: 4505
BsiEI CG_ry'CG
Cuts at: 104 2047 2093 3301 104
Size: 1943 46 1208 1308
Fragments arranged by size:
1943 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 542 793 947 1288 1519 2297 2487 3279 542
Size: 251 154 341 231 778 190 792 1768
Fragments arranged by size:
1768 792 778 341 251 231 190 154
BsmI GAATG_Cn'
Cuts at: 1449 1449
Size: 4505
BsmBI CGTCTCn'nnnn_
Cuts at: 800 800
Size: 4505
Bsp1286I G_dGCh'C
Cuts at: 542 599 677 793 947 998 1288 1472 1519
Size: 57 78 116 154 51 290 184 47
Cuts at: 1519 2130 2223 2297 2487 2549 3279 542
Size: 611 93 74 190 62 730 1768
Fragments arranged by size:
1768 730 611 290 190 184 154 116
93 78 74 62 57 51 47
BspEI T'CCGG_A
Cuts at: 3725 3725
Size: 4505
BspHI T'CATG_A
Cuts at: 449 3681 449
Size: 3232 1273
BspMI ACCTGCnnnn'nnnn_
Cuts at: 603 1555 1769 2071 2452 603
Size: 952 214 302 381 2656
Fragments arranged by size:
2656 952 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2797 2851 4335 4500 50
Size: 2747 54 1484 165 55
Fragments arranged by size:
2747 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2417 2417
Size: 4505
BsrFI r'CCGG_y
Cuts at: 2503 2684 2893 2503
Size: 181 209 4115
Fragments arranged by size:
4115 209 181
BsrGI T'GTAC_A
Cuts at: 881 1130 881
Size: 249 4256
BssSI C'ACGA_G
Cuts at: 2776 3134 2776
Size: 358 4147
BstAPI GCAn_nnn'nTGC
Cuts at: 1882 1882
Size: 4505
BstBI TT'CG_AA
Cuts at: 110 2866 110
Size: 2756 1749
BstEII G'GTnAC_C
Cuts at: 312 312
Size: 4505
BstXI CCAn_nnnn'nTGG
Cuts at: 973 973
Size: 4505
BstYI r'GATC_y
Cuts at: 1 519 1530 1997 2020 2355 2601 3613 3699
Size: 518 1011 467 23 335 246 1012 86
Cuts at: 3699 3711 4057 1
Size: 12 346 449
Fragments arranged by size:
1012 1011 518 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 179 1418 179
Size: 1239 3266
BtgI C'CryG_G
Cuts at: 125 948 1173 125
Size: 823 225 3457
Fragments arranged by size:
3457 823 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2455 2636 2799 2455
Size: 181 163 4161
Fragments arranged by size:
4161 181 163
BtsI GCAGTG_nn'
Cuts at: 1748 1748
Size: 4505
DraI TTT'AAA
Cuts at: 1504 1504
Size: 4505
DrdI GACnn_nn'nnGTC
Cuts at: 2211 3069 2211
Size: 858 3647
EaeI y'GGCC_r
Cuts at: 743 975 1382 2090 2264 2655 2682 4027 743
Size: 232 407 708 174 391 27 1345 1221
Fragments arranged by size:
1345 1221 708 407 391 232 174 27
EagI C'GGCC_G
Cuts at: 2090 2090
Size: 4505
EarI CTCTTCn'nnn_
Cuts at: 302 436 2528 2738 302
Size: 134 2092 210 2069
Fragments arranged by size:
2092 2069 210 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 168 2713 3023 3169 4315 168
Size: 2545 310 146 1146 358
Fragments arranged by size:
2545 1146 358 310 146
EcoICRI GAG'CTC
Cuts at: 945 1517 945
Size: 572 3933
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1456 2330 2762 3509 3777 286
Size: 1170 874 432 747 268 1014
Fragments arranged by size:
1170 1014 874 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 367 481 595 673 674 732 994 995 1428
Size: 114 114 78 1 58 262 1 433
Cuts at: 1428 1644 367
Size: 216 3228
Fragments arranged by size:
3228 433 262 216 114 114 78 58
1 1
EcoRI G'AATT_C
Cuts at: 1509 1509
Size: 4505
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4505
FspI TGC'GCA
Cuts at: 2286 2286
Size: 4505
HaeII r_GCGC'y
Cuts at: 927 1939 2187 3209 4188 927
Size: 1012 248 1022 979 1244
Fragments arranged by size:
1244 1022 1012 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 367 399 695 727 856 888 2347
Size: 32 133 32 296 32 129 32 1459
Cuts at: 2347 2377 2379 2409 202
Size: 30 2 30 2298
Fragments arranged by size:
2298 1459 296 133 129 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 345 1544 345
Size: 1199 3306
KasI G'GCGC_C
Cuts at: 923 1935 2183 923
Size: 1012 248 3245
Fragments arranged by size:
3245 1012 248
KpnI G_GTAC'C
Cuts at: 1525 1525
Size: 4505
MfeI C'AATT_G
Cuts at: 202 202
Size: 4505
MluI A'CGCG_T
Cuts at: 3938 3938
Size: 4505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1338 3176 3360 1338
Size: 1838 184 2483
Fragments arranged by size:
2483 1838 184
MscI TGG'CCA
Cuts at: 745 977 1384 2266 745
Size: 232 407 882 2984
Fragments arranged by size:
2984 882 407 232
MslI CAynn'nnrTG
Cuts at: 454 1126 2494 2621 454
Size: 672 1368 127 2338
Fragments arranged by size:
2338 1368 672 127
MspA1I CmG'CkG
Cuts at: 630 897 912 920 1308 1616 1930 2290 3303
Size: 267 15 8 388 308 314 360 1013
Cuts at: 3303 3548 630
Size: 245 1587
Fragments arranged by size:
1587 1013 388 360 314 308 267 245
15 8
NaeI GCC'GGC
Cuts at: 2686 2686
Size: 4505
NarI GG'CG_CC
Cuts at: 924 1936 2184 924
Size: 1012 248 3245
Fragments arranged by size:
3245 1012 248
NcoI C'CATG_G
Cuts at: 125 948 1173 125
Size: 823 225 3457
Fragments arranged by size:
3457 823 225
NgoMIV G'CCGG_C
Cuts at: 2684 2684
Size: 4505
NspI r_CATG'y
Cuts at: 336 1033 1164 1558 2058 2965 336
Size: 697 131 394 500 907 1876
Fragments arranged by size:
1876 907 697 500 394 131
PasI CC'CwG_GG
Cuts at: 592 592
Size: 4505
PciI A'CATG_T
Cuts at: 1160 2961 1160
Size: 1801 2704
PflMI CCAn_nnn'nTGG
Cuts at: 684 684
Size: 4505
PfoI T'CCnGG_A
Cuts at: 860 1403 1472 1481 860
Size: 543 69 9 3884
Fragments arranged by size:
3884 543 69 9
PmeI GTTT'AAAC
Cuts at: 1504 1504
Size: 4505
PmlI CAC'GTG
Cuts at: 543 1283 543
Size: 740 3765
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 147 179 3644 3676 147
Size: 32 3465 32 976
Fragments arranged by size:
3465 976 32 32
PpuMI rG'GwC_Cy
Cuts at: 367 481 732 367
Size: 114 251 4140
Fragments arranged by size:
4140 251 114
PspOMI G'GGCC_C
Cuts at: 595 673 994 595
Size: 78 321 4106
Fragments arranged by size:
4106 321 78
PspXI vC'TCGA_Gb
Cuts at: 1012 1012
Size: 4505
PstI C_TGCA'G
Cuts at: 1552 2237 1552
Size: 685 3820
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4505
PvuII CAG'CTG
Cuts at: 630 897 1308 1930 2290 630
Size: 267 411 622 360 2845
Fragments arranged by size:
2845 622 411 360 267
RsrII CG'GwC_CG
Cuts at: 2700 2700
Size: 4505
SacI G_AGCT'C
Cuts at: 947 1519 947
Size: 572 3933
SalI G'TCGA_C
Cuts at: 343 1542 343
Size: 1199 3306
SanDI GG'GwC_CC
Cuts at: 367 732 367
Size: 365 4140
SapI GCTCTTCn'nnn_
Cuts at: 302 2528 2738 302
Size: 2226 210 2069
Fragments arranged by size:
2226 2069 210
SbfI CC_TGCA'GG
Cuts at: 1552 1552
Size: 4505
ScaI AGT'ACT
Cuts at: 4022 4022
Size: 4505
SfcI C'TryA_G
Cuts at: 33 274 1548 2233 3226 3417 33
Size: 241 1274 685 993 191 1121
Fragments arranged by size:
1274 1121 993 685 241 191
SfoI GGC'GCC
Cuts at: 925 1937 2185 925
Size: 1012 248 3245
Fragments arranged by size:
3245 1012 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4505
SmaI CCC'GGG
Cuts at: 607 1527 607
Size: 920 3585
SmlI C'TyrA_G
Cuts at: 1012 1660 2575 3067 3329 1012
Size: 648 915 492 262 2188
Fragments arranged by size:
2188 915 648 492 262
SnaBI TAC'GTA
Cuts at: 1497 1497
Size: 4505
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4505
SphI G_CATG'C
Cuts at: 336 1558 2058 336
Size: 1222 500 2783
Fragments arranged by size:
2783 1222 500
StyI C'CwwG_G
Cuts at: 125 948 1173 1385 1639 1993 125
Size: 823 225 212 254 354 2637
Fragments arranged by size:
2637 823 354 254 225 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 711 716 1227 2088 2221 2680 2889 711
Size: 5 511 861 133 459 209 2327
Fragments arranged by size:
2327 861 511 459 209 133 5
TatI w'GTAC_w
Cuts at: 881 1130 1163 4020 881
Size: 249 33 2857 1366
Fragments arranged by size:
2857 1366 249 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 465 497 946 978 4022 4054 465
Size: 32 449 32 3044 32 916
Fragments arranged by size:
3044 916 449 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2302 2302
Size: 4505
XbaI T'CTAG_A
Cuts at: 1536 1536
Size: 4505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2000 2000
Size: 4505
XhoI C'TCGA_G
Cuts at: 1012 1012
Size: 4505
XmaI C'CCGG_G
Cuts at: 605 1525 605
Size: 920 3585
XmnI GAAnn'nnTTC
Cuts at: 296 296
Size: 4505
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI BsiWI BssHII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PsrI SacII SexAI SfiI SgrAI SrfI
SspI StuI SwaI ZraI