Restriction map for
pF1KSDA1612
(Circular) (Six-base) MAPSORT of: pF1KSDA1612.seq Check: 6214 from: 1 to: 4763
pF1KSDA1612 4763 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 228 228
Size: 4763
AccI GT'mk_AC
Cuts at: 1634 1801 1634
Size: 167 4596
Acc65I G'GTAC_C
Cuts at: 664 1779 664
Size: 1115 3648
AclI AA'CG_TT
Cuts at: 4603 4603
Size: 4763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 809 821 1030 1234 1310 2588 3020 3767
Size: 463 12 209 204 76 1278 432 747
Cuts at: 3767 4035 346
Size: 268 1074
Fragments arranged by size:
1278 1074 747 463 432 268 209 204
76 12
AflII C'TTAA_G
Cuts at: 1021 1021
Size: 4763
AflIII A'CryG_T
Cuts at: 545 1068 2115 3219 4196 545
Size: 523 1047 1104 977 1112
Fragments arranged by size:
1112 1104 1047 977 523
AgeI A'CCGG_T
Cuts at: 1456 3151 1456
Size: 1695 3068
AlwNI CAG_nnn'CTG
Cuts at: 2068 3635 2068
Size: 1567 3196
ApaLI G'TGCA_C
Cuts at: 3533 3533
Size: 4763
ApoI r'AATT_y
Cuts at: 476 565 971 1001 1033 1495 1657 1767 4116
Size: 89 406 30 32 462 162 110 2349
Cuts at: 4116 4372 476
Size: 256 867
Fragments arranged by size:
2349 867 462 406 256 162 110 89
32 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4763
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
AvaI C'yCGr_G
Cuts at: 161 1783 161
Size: 1622 3141
BamHI G'GATC_C
Cuts at: 1788 1788
Size: 4763
BanI G'GyrC_C
Cuts at: 608 664 1779 2193 2441 2476 608
Size: 56 1115 414 248 35 2895
Fragments arranged by size:
2895 1115 414 248 56 35
BanII G_rGCy'C
Cuts at: 152 895 1777 2807 152
Size: 743 882 1030 2108
Fragments arranged by size:
2108 1030 882 743
BbeI G_GCGC'C
Cuts at: 612 2197 2445 612
Size: 1585 248 2930
Fragments arranged by size:
2930 1585 248
BbsI GAAGACnn'nnnn_
Cuts at: 1459 1459
Size: 4763
BbvCI CC'TCA_GC
Cuts at: 245 1121 245
Size: 876 3887
BciVI GTATCCnnnnn_n'
Cuts at: 2661 3422 4763 2661
Size: 761 1341 2661
Fragments arranged by size:
2661 1341 761
BclI T'GATC_A
Cuts at: 2283 2283
Size: 4763
BglII A'GATC_T
Cuts at: 2278 4315 2278
Size: 2037 2726
BlpI GC'TnA_GC
Cuts at: 1875 1875
Size: 4763
Bme1580I G_kGCm'C
Cuts at: 2388 2481 3537 2388
Size: 93 1056 3614
Fragments arranged by size:
3614 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1975 2056 2391 4535 1975
Size: 81 335 2144 2203
Fragments arranged by size:
2203 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 4763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 273
Size: 4763
Bpu10I CC'TnA_GC
Cuts at: 245 1121 1316 245
Size: 876 195 3692
Fragments arranged by size:
3692 876 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1939 2818 3310 3608 1939
Size: 879 492 298 3094
Fragments arranged by size:
3094 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4467 4467
Size: 4763
BsaAI yAC'GTr
Cuts at: 1755 2116 1755
Size: 361 4402
BsaBI GATnn'nnATC
Cuts at: 6 1339 2277 4314 6
Size: 1333 938 2037 455
Fragments arranged by size:
2037 1333 938 455
BsaHI Gr'CG_yC
Cuts at: 265 609 2194 2442 3144 4640 265
Size: 344 1585 248 702 1496 388
Fragments arranged by size:
1585 1496 702 388 344 248
BsaWI w'CCGG_w
Cuts at: 494 1456 1952 2473 3151 3425 3572 3983 494
Size: 962 496 521 678 274 147 411 1274
Fragments arranged by size:
1274 962 678 521 496 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 645 675 843 873 645
Size: 30 168 30 4535
Fragments arranged by size:
4535 168 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1138 1138
Size: 4763
BseYI C'CCAG_C
Cuts at: 156 1535 2188 2946 3523 4019 156
Size: 1379 653 758 577 496 900
Fragments arranged by size:
1379 900 758 653 577 496
BsiEI CG_ry'CG
Cuts at: 104 2305 2351 3559 104
Size: 2201 46 1208 1308
Fragments arranged by size:
2201 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1777 2555 2745 3537 1777
Size: 778 190 792 3003
Fragments arranged by size:
3003 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 152 895 1777 2388 2481 2555 2745 2807 3537
Size: 743 882 611 93 74 190 62 730
Cuts at: 3537 152
Size: 1378
Fragments arranged by size:
1378 882 743 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3983 3983
Size: 4763
BspHI T'CATG_A
Cuts at: 912 1476 3939 912
Size: 564 2463 1736
Fragments arranged by size:
2463 1736 564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 228 1813 2027 2329 2710 228
Size: 1585 214 302 381 2281
Fragments arranged by size:
2281 1585 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3055 3109 4593 4758 50
Size: 3005 54 1484 165 55
Fragments arranged by size:
3005 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1371 1568 2675 1371
Size: 197 1107 3459
Fragments arranged by size:
3459 1107 197
BsrFI r'CCGG_y
Cuts at: 1456 2761 2942 3151 1456
Size: 1305 181 209 3068
Fragments arranged by size:
3068 1305 209 181
BsrGI T'GTAC_A
Cuts at: 677 677
Size: 4763
BssSI C'ACGA_G
Cuts at: 3034 3392 3034
Size: 358 4405
BstAPI GCAn_nnn'nTGC
Cuts at: 2140 2140
Size: 4763
BstBI TT'CG_AA
Cuts at: 110 3124 110
Size: 3014 1749
BstYI r'GATC_y
Cuts at: 1 1788 2255 2278 2613 2859 3871 3957 3969
Size: 1787 467 23 335 246 1012 86 12
Cuts at: 3969 4315 1
Size: 346 449
Fragments arranged by size:
1787 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2713 2894 3057 2713
Size: 181 163 4419
Fragments arranged by size:
4419 181 163
BtsI GCAGTG_nn'
Cuts at: 2006 2006
Size: 4763
DraI TTT'AAA
Cuts at: 1762 1762
Size: 4763
DraIII CAC_nnn'GTG
Cuts at: 318 783 318
Size: 465 4298
DrdI GACnn_nn'nnGTC
Cuts at: 2469 3327 2469
Size: 858 3905
EaeI y'GGCC_r
Cuts at: 139 1337 2348 2522 2913 2940 4285 139
Size: 1198 1011 174 391 27 1345 617
Fragments arranged by size:
1345 1198 1011 617 391 174 27
EagI C'GGCC_G
Cuts at: 2348 2348
Size: 4763
EarI CTCTTCn'nnn_
Cuts at: 436 441 974 1583 2786 2996 436
Size: 5 533 609 1203 210 2203
Fragments arranged by size:
2203 1203 609 533 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 211 2971 3281 3427 4573 211
Size: 2760 310 146 1146 401
Fragments arranged by size:
2760 1146 401 310 146
EcoICRI GAG'CTC
Cuts at: 1775 1775
Size: 4763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 346 809 821 1030 1234 1310 2588 3020
Size: 73 463 12 209 204 76 1278 432
Cuts at: 3020 3767 4035 273
Size: 747 268 1001
Fragments arranged by size:
1278 1001 747 463 432 268 209 204
76 73 12
EcoNI CCTnn'n_nnAGG
Cuts at: 648 648
Size: 4763
EcoO109I rG'GnC_Cy
Cuts at: 1090 1902 1090
Size: 812 3951
EcoRI G'AATT_C
Cuts at: 565 1767 565
Size: 1202 3561
EcoRV GAT'ATC
Cuts at: 1284 1284
Size: 4763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1637 1719 2782 1637
Size: 82 1063 3618
Fragments arranged by size:
3618 1063 82
FspI TGC'GCA
Cuts at: 2544 2544
Size: 4763
HaeII r_GCGC'y
Cuts at: 612 2197 2445 3467 4446 612
Size: 1585 248 1022 979 929
Fragments arranged by size:
1585 1022 979 929 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 517 549 843 875 2605 2635 2637
Size: 32 4 32 294 32 1730 30 2
Cuts at: 2637 2667 481
Size: 30 2577
Fragments arranged by size:
2577 1730 294 32 32 32 30 30
4 2
HincII GTy'rAC
Cuts at: 1802 1802
Size: 4763
HindIII A'AGCT_T
Cuts at: 1132 1216 1132
Size: 84 4679
KasI G'GCGC_C
Cuts at: 608 2193 2441 608
Size: 1585 248 2930
Fragments arranged by size:
2930 1585 248
KpnI G_GTAC'C
Cuts at: 668 1783 668
Size: 1115 3648
MluI A'CGCG_T
Cuts at: 4196 4196
Size: 4763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1223 3434 3618 1223
Size: 2211 184 2368
Fragments arranged by size:
2368 2211 184
MscI TGG'CCA
Cuts at: 1339 2524 1339
Size: 1185 3578
MslI CAynn'nnrTG
Cuts at: 289 295 544 593 1067 2752 2879 289
Size: 6 249 49 474 1685 127 2173
Fragments arranged by size:
2173 1685 474 249 127 49 6
MspA1I CmG'CkG
Cuts at: 145 1874 2188 2548 3561 3806 145
Size: 1729 314 360 1013 245 1102
Fragments arranged by size:
1729 1102 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2944 2944
Size: 4763
NarI GG'CG_CC
Cuts at: 609 2194 2442 609
Size: 1585 248 2930
Fragments arranged by size:
2930 1585 248
NgoMIV G'CCGG_C
Cuts at: 2942 2942
Size: 4763
NspI r_CATG'y
Cuts at: 549 1072 1816 2316 3223 549
Size: 523 744 500 907 2089
Fragments arranged by size:
2089 907 744 523 500
PasI CC'CwG_GG
Cuts at: 644 644
Size: 4763
PciI A'CATG_T
Cuts at: 545 1068 3219 545
Size: 523 2151 2089
Fragments arranged by size:
2151 2089 523
PflMI CCAn_nnn'nTGG
Cuts at: 1549 1549
Size: 4763
PmeI GTTT'AAAC
Cuts at: 1762 1762
Size: 4763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4731
PpuMI rG'GwC_Cy
Cuts at: 1090 1090
Size: 4763
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 866 898 866
Size: 32 4731
PstI C_TGCA'G
Cuts at: 1810 2495 1810
Size: 685 4078
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4763
PvuII CAG'CTG
Cuts at: 2188 2548 2188
Size: 360 4403
RsrII CG'GwC_CG
Cuts at: 2958 2958
Size: 4763
SacI G_AGCT'C
Cuts at: 1777 1777
Size: 4763
SalI G'TCGA_C
Cuts at: 1800 1800
Size: 4763
SapI GCTCTTCn'nnn_
Cuts at: 1583 2786 2996 1583
Size: 1203 210 3350
Fragments arranged by size:
3350 1203 210
SbfI CC_TGCA'GG
Cuts at: 1810 1810
Size: 4763
ScaI AGT'ACT
Cuts at: 4280 4280
Size: 4763
SexAI A'CCwGG_T
Cuts at: 632 779 632
Size: 147 4616
SfcI C'TryA_G
Cuts at: 33 1635 1806 2491 3484 3675 33
Size: 1602 171 685 993 191 1121
Fragments arranged by size:
1602 1121 993 685 191 171
SfoI GGC'GCC
Cuts at: 610 2195 2443 610
Size: 1585 248 2930
Fragments arranged by size:
2930 1585 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4763
SmaI CCC'GGG
Cuts at: 163 1785 163
Size: 1622 3141
SmlI C'TyrA_G
Cuts at: 1021 1918 2833 3325 3587 1021
Size: 897 915 492 262 2197
Fragments arranged by size:
2197 915 897 492 262
SnaBI TAC'GTA
Cuts at: 1755 1755
Size: 4763
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4763
SphI G_CATG'C
Cuts at: 1816 2316 1816
Size: 500 4263
StuI AGG'CCT
Cuts at: 250 1054 1211 250
Size: 804 157 3802
Fragments arranged by size:
3802 804 157
StyI C'CwwG_G
Cuts at: 1055 1431 1897 2251 1055
Size: 376 466 354 3567
Fragments arranged by size:
3567 466 376 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 649 2346 2479 2938 3147 649
Size: 1697 133 459 209 2265
Fragments arranged by size:
2265 1697 459 209 133
TatI w'GTAC_w
Cuts at: 677 1517 4278 677
Size: 840 2761 1162
Fragments arranged by size:
2761 1162 840
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 645 677 4280 4312 645
Size: 32 3603 32 1096
Fragments arranged by size:
3603 1096 32 32
Tth111I GACn'n_nGTC
Cuts at: 2560 2560
Size: 4763
XbaI T'CTAG_A
Cuts at: 1794 1794
Size: 4763
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2258 2258
Size: 4763
XmaI C'CCGG_G
Cuts at: 161 1783 161
Size: 1622 3141
XmnI GAAnn'nnTTC
Cuts at: 359 695 1702 359
Size: 336 1007 3420
Fragments arranged by size:
3420 1007 336
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI
SrfI SspI SwaI XhoI ZraI