Restriction map for
pF1KSDA1614
(Circular) (Six-base) MAPSORT of: pF1KSDA1614.seq Check: 6122 from: 1 to: 6707
pF1KSDA1614 6707 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2162 2162
Size: 6707
AatII G_ACGT'C
Cuts at: 3247 3247
Size: 6707
AccI GT'mk_AC
Cuts at: 3745 3745
Size: 6707
Acc65I G'GTAC_C
Cuts at: 3723 3723
Size: 6707
AclI AA'CG_TT
Cuts at: 1052 6547 1052
Size: 5495 1212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 2237 2555 4532 4964 5711 5979 2122
Size: 115 318 1977 432 747 268 2850
Fragments arranged by size:
2850 1977 747 432 318 268 115
AfeI AGC'GCT
Cuts at: 949 3538 949
Size: 2589 4118
AflIII A'CryG_T
Cuts at: 3030 4059 5163 6140 3030
Size: 1029 1104 977 3597
Fragments arranged by size:
3597 1104 1029 977
AgeI A'CCGG_T
Cuts at: 3359 5095 3359
Size: 1736 4971
AhdI GACnn_n'nnGTC
Cuts at: 1193 1193
Size: 6707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1180 1579 1180
Size: 399 6308
AlwNI CAG_nnn'CTG
Cuts at: 538 1551 2641 2687 3022 3058 4012 5579 538
Size: 1013 1090 46 335 36 954 1567 1666
Fragments arranged by size:
1666 1567 1090 1013 954 335 46 36
ApaI G_GGCC'C
Cuts at: 182 364 740 1459 1585 2842 3261 182
Size: 182 376 719 126 1257 419 3628
Fragments arranged by size:
3628 1257 719 419 376 182 126
ApaLI G'TGCA_C
Cuts at: 5477 5477
Size: 6707
ApoI r'AATT_y
Cuts at: 3711 6060 6316 3711
Size: 2349 256 4102
Fragments arranged by size:
4102 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6707
AvaI C'yCGr_G
Cuts at: 1152 1352 2087 2783 3727 1152
Size: 200 735 696 944 4132
Fragments arranged by size:
4132 944 735 696 200
AvrII C'CTAG_G
Cuts at: 2158 2158
Size: 6707
BamHI G'GATC_C
Cuts at: 1904 3732 1904
Size: 1828 4879
BanI G'GyrC_C
Cuts at: 1156 1701 1710 1730 1782 2544 2735 3514 3584
Size: 545 9 20 52 762 191 779 70
Cuts at: 3584 3723 4137 4385 4420 1156
Size: 139 414 248 35 3443
Fragments arranged by size:
3443 779 762 545 414 248 191 139
70 52 35 20 9
BanII G_rGCy'C
Cuts at: 182 364 679 740 760 1069 1222 1459 1585
Size: 182 315 61 20 309 153 237 126
Cuts at: 1585 1629 1814 1858 1886 2080 2126 2801 2842
Size: 44 185 44 28 194 46 675 41
Cuts at: 2842 3261 3721 4751 182
Size: 419 460 1030 2138
Fragments arranged by size:
2138 1030 675 460 419 315 309 237
194 185 182 153 126 61 46 44
44 41 28 20
BbeI G_GCGC'C
Cuts at: 1786 2548 3588 4141 4389 1786
Size: 762 1040 553 248 4104
Fragments arranged by size:
4104 1040 762 553 248
BbvCI CC'TCA_GC
Cuts at: 222 2114 3617 222
Size: 1892 1503 3312
Fragments arranged by size:
3312 1892 1503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1734 1768 1734
Size: 34 6673
BciVI GTATCCnnnnn_n'
Cuts at: 2659 4605 5366 6707 2659
Size: 1946 761 1341 2659
Fragments arranged by size:
2659 1946 1341 761
BclI T'GATC_A
Cuts at: 4227 4227
Size: 6707
BglI GCCn_nnn'nGGC
Cuts at: 573 1334 573
Size: 761 5946
BglII A'GATC_T
Cuts at: 882 4222 6259 882
Size: 3340 2037 1330
Fragments arranged by size:
3340 2037 1330
BlpI GC'TnA_GC
Cuts at: 1216 1552 2910 3343 3819 1216
Size: 336 1358 433 476 4104
Fragments arranged by size:
4104 1358 476 433 336
Bme1580I G_kGCm'C
Cuts at: 182 364 740 1159 1260 1310 1403 1442 1459
Size: 182 376 419 101 50 93 39 17
Cuts at: 1459 1585 1939 2842 3261 3441 3553 4332 4425
Size: 126 354 903 419 180 112 779 93
Cuts at: 4425 5481 182
Size: 1056 1408
Fragments arranged by size:
1408 1056 903 779 419 419 376 354
182 180 126 112 101 93 93 50
39 17
BmgBI CAC'GTC
Cuts at: 655 3456 655
Size: 2801 3906
BmrI ACTGGGnnnn_n'
Cuts at: 1155 2283 3919 4000 4335 6479 1155
Size: 1128 1636 81 335 2144 1383
Fragments arranged by size:
2144 1636 1383 1128 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 237 370 1405 1968 5074 237
Size: 133 1035 563 3106 1870
Fragments arranged by size:
3106 1870 1035 563 133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 671 972 980 988 1375 1401 1540 1789
Size: 331 301 8 8 387 26 139 249
Cuts at: 1789 1798 2064 2281 2890 3044 340
Size: 9 266 217 609 154 4003
Fragments arranged by size:
4003 609 387 331 301 266 249 217
154 139 26 9 8 8
Bpu10I CC'TnA_GC
Cuts at: 222 245 1852 2114 2617 3617 3640 222
Size: 23 1607 262 503 1000 23 3289
Fragments arranged by size:
3289 1607 1000 503 262 23 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4762 5254 5552 3883
Size: 879 492 298 5038
Fragments arranged by size:
5038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 956 6411 956
Size: 5455 1252
BsaAI yAC'GTr
Cuts at: 1473 3699 4060 1473
Size: 2226 361 4120
Fragments arranged by size:
4120 2226 361
BsaBI GATnn'nnATC
Cuts at: 6 4221 6258 6
Size: 4215 2037 455
Fragments arranged by size:
4215 2037 455
BsaHI Gr'CG_yC
Cuts at: 1783 2386 2545 3244 3585 4138 4386 5088 6584
Size: 603 159 699 341 553 248 702 1496
Cuts at: 6584 1783
Size: 1906
Fragments arranged by size:
1906 1496 702 699 603 553 341 248
159
BsaWI w'CCGG_w
Cuts at: 2856 3359 3896 4417 5095 5369 5516 5927 2856
Size: 503 537 521 678 274 147 411 3636
Fragments arranged by size:
3636 678 537 521 503 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1853 1883 1916 1946 2959 2989 3017 3047 1853
Size: 30 33 30 1013 30 28 30 5513
Fragments arranged by size:
5513 1013 33 30 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 539 613 925 1740 2017 2980 3599 539
Size: 74 312 815 277 963 619 3647
Fragments arranged by size:
3647 963 815 619 312 277 74
BseYI C'CCAG_C
Cuts at: 326 732 739 826 939 982 1347 1842 2027
Size: 406 7 87 113 43 365 495 185
Cuts at: 2027 2144 2310 2540 2622 2711 2716 3186 3466
Size: 117 166 230 82 89 5 470 280
Cuts at: 3466 3629 4132 4890 5467 5963 326
Size: 163 503 758 577 496 1070
Fragments arranged by size:
1070 758 577 503 496 495 470 406
365 280 230 185 166 163 117 113
89 87 82 43 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1100 3262 3422 1100
Size: 2162 160 4385
Fragments arranged by size:
4385 2162 160
BsiEI CG_ry'CG
Cuts at: 104 4249 4295 5503 104
Size: 4145 46 1208 1308
Fragments arranged by size:
4145 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1558 1814 1858 2126 3465 3721 4499 4689 5481
Size: 256 44 268 1339 256 778 190 792
Cuts at: 5481 1558
Size: 2784
Fragments arranged by size:
2784 1339 792 778 268 256 256 190
44
Bsp1286I G_dGCh'C
Cuts at: 182 364 679 740 760 1069 1159 1222 1260
Size: 182 315 61 20 309 90 63 38
Cuts at: 1260 1310 1403 1442 1459 1558 1585 1629 1814
Size: 50 93 39 17 99 27 44 185
Cuts at: 1814 1858 1886 1939 2080 2126 2801 2842 3261
Size: 44 28 53 141 46 675 41 419
Cuts at: 3261 3441 3465 3553 3721 4332 4425 4499 4689
Size: 180 24 88 168 611 93 74 190
Cuts at: 4689 4751 5481 182
Size: 62 730 1408
Fragments arranged by size:
1408 730 675 611 419 315 309 190
185 182 180 168 141 99 93 93
90 88 74 63 62 61 53 50
46 44 44 41 39 38 28 27
24 20 17
BspEI T'CCGG_A
Cuts at: 5927 5927
Size: 6707
BspHI T'CATG_A
Cuts at: 5883 5883
Size: 6707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 572 2162 3757 3971 4273 4654 572
Size: 1590 1595 214 302 381 2625
Fragments arranged by size:
2625 1595 1590 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1524 4999 5053 6537 6702 50
Size: 1474 3475 54 1484 165 55
Fragments arranged by size:
3475 1484 1474 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4619 4619
Size: 6707
BsrFI r'CCGG_y
Cuts at: 1707 3359 3414 4705 4886 5095 1707
Size: 1652 55 1291 181 209 3319
Fragments arranged by size:
3319 1652 1291 209 181 55
BssSI C'ACGA_G
Cuts at: 1404 2434 2818 4978 5336 1404
Size: 1030 384 2160 358 2775
Fragments arranged by size:
2775 2160 1030 384 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1044 1248 2154 2742 2920 4084 1044
Size: 204 906 588 178 1164 3667
Fragments arranged by size:
3667 1164 906 588 204 178
BstBI TT'CG_AA
Cuts at: 110 5068 110
Size: 4958 1749
BstEII G'GTnAC_C
Cuts at: 2626 2626
Size: 6707
BstXI CCAn_nnnn'nTGG
Cuts at: 210 281 318 210
Size: 71 37 6599
Fragments arranged by size:
6599 71 37
BstYI r'GATC_y
Cuts at: 1 882 1904 3019 3732 4199 4222 4557 4803
Size: 881 1022 1115 713 467 23 335 246
Cuts at: 4803 5815 5901 5913 6259 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1115 1022 1012 881 713 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 293 2726 293
Size: 2433 4274
BtgI C'CryG_G
Cuts at: 125 158 209 679 765 1571 2936 3430 3482
Size: 33 51 470 86 806 1365 494 52
Cuts at: 3482 3624 125
Size: 142 3208
Fragments arranged by size:
3208 1365 806 494 470 142 86 52
51 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4657 4838 5001 4657
Size: 181 163 6363
Fragments arranged by size:
6363 181 163
BtsI GCAGTG_nn'
Cuts at: 996 2740 3950 996
Size: 1744 1210 3753
Fragments arranged by size:
3753 1744 1210
DraI TTT'AAA
Cuts at: 3706 3706
Size: 6707
DraIII CAC_nnn'GTG
Cuts at: 1934 3285 3436 1934
Size: 1351 151 5205
Fragments arranged by size:
5205 1351 151
DrdI GACnn_nn'nnGTC
Cuts at: 843 3249 4413 5271 843
Size: 2406 1164 858 2279
Fragments arranged by size:
2406 2279 1164 858
EaeI y'GGCC_r
Cuts at: 149 413 1176 1335 3416 4292 4466 4857 4884
Size: 264 763 159 2081 876 174 391 27
Cuts at: 4884 6229 149
Size: 1345 627
Fragments arranged by size:
2081 1345 876 763 627 391 264 174
159 27
EagI C'GGCC_G
Cuts at: 4292 4292
Size: 6707
EarI CTCTTCn'nnn_
Cuts at: 3157 3617 4730 4940 3157
Size: 460 1113 210 4924
Fragments arranged by size:
4924 1113 460 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1482 2055 2134 4915 5225 5371 6517 1482
Size: 573 79 2781 310 146 1146 1672
Fragments arranged by size:
2781 1672 1146 573 310 146 79
EcoICRI GAG'CTC
Cuts at: 1812 1856 2124 3719 1812
Size: 44 268 1595 4800
Fragments arranged by size:
4800 1595 268 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 671 972 980 988 1375 1401 1540 1789
Size: 331 301 8 8 387 26 139 249
Cuts at: 1789 1798 2064 2122 2237 2281 2555 2890 3044
Size: 9 266 58 115 44 274 335 154
Cuts at: 3044 4532 4964 5711 5979 340
Size: 1488 432 747 268 1068
Fragments arranged by size:
1488 1068 747 432 387 335 331 301
274 268 266 249 154 139 115 58
44 26 9 8 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2303 3496 2303
Size: 1193 5514
EcoO109I rG'GnC_Cy
Cuts at: 178 218 360 361 622 736 793 845 1274
Size: 40 142 1 261 114 57 52 429
Cuts at: 1274 1356 1455 1456 1581 1582 1799 2012 2133
Size: 82 99 1 125 1 217 213 121
Cuts at: 2133 2284 2684 2838 2839 2890 3067 3257 3258
Size: 151 400 154 1 51 177 190 1
Cuts at: 3258 3656 3846 178
Size: 398 190 3039
Fragments arranged by size:
3039 429 400 398 261 217 213 190
190 177 154 151 142 125 121 114
99 82 57 52 51 40 1 1
1 1 1
EcoRI G'AATT_C
Cuts at: 3711 3711
Size: 6707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4726 4726
Size: 6707
FseI GG_CCGG'CC
Cuts at: 3418 3418
Size: 6707
FspI TGC'GCA
Cuts at: 4488 4488
Size: 6707
HaeII r_GCGC'y
Cuts at: 951 983 1539 1545 1786 2548 3540 3588 4141
Size: 32 556 6 241 762 992 48 553
Cuts at: 4141 4389 5411 6390 951
Size: 248 1022 979 1268
Fragments arranged by size:
1268 1022 992 979 762 556 553 248
241 48 32 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 442 474 880 912 1135 1167 1179 1211 1474
Size: 32 406 32 223 32 12 32 263
Cuts at: 1474 1506 2959 2991 4549 4579 4581 4611 442
Size: 32 1453 32 1558 30 2 30 2538
Fragments arranged by size:
2538 1558 1453 406 263 223 32 32
32 32 32 32 30 30 12 2
HincII GTy'rAC
Cuts at: 3746 3746
Size: 6707
KasI G'GCGC_C
Cuts at: 1782 2544 3584 4137 4385 1782
Size: 762 1040 553 248 4104
Fragments arranged by size:
4104 1040 762 553 248
KpnI G_GTAC'C
Cuts at: 3727 3727
Size: 6707
MluI A'CGCG_T
Cuts at: 6140 6140
Size: 6707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3286 5378 5562 3286
Size: 2092 184 4431
Fragments arranged by size:
4431 2092 184
MscI TGG'CCA
Cuts at: 415 1337 4468 415
Size: 922 3131 2654
Fragments arranged by size:
3131 2654 922
MslI CAynn'nnrTG
Cuts at: 4696 4823 4696
Size: 127 6580
MspA1I CmG'CkG
Cuts at: 160 226 255 579 729 1114 1421 1433 1573
Size: 66 29 324 150 385 307 12 140
Cuts at: 1573 1748 3406 3525 3531 3818 4132 4492 5505
Size: 175 1658 119 6 287 314 360 1013
Cuts at: 5505 5750 160
Size: 245 1117
Fragments arranged by size:
1658 1117 1013 385 360 324 314 307
287 245 175 150 140 119 66 29
12 6
NaeI GCC'GGC
Cuts at: 1709 3416 4888 1709
Size: 1707 1472 3528
Fragments arranged by size:
3528 1707 1472
NarI GG'CG_CC
Cuts at: 1783 2545 3585 4138 4386 1783
Size: 762 1040 553 248 4104
Fragments arranged by size:
4104 1040 762 553 248
NcoI C'CATG_G
Cuts at: 125 765 3624 125
Size: 640 2859 3208
Fragments arranged by size:
3208 2859 640
NgoMIV G'CCGG_C
Cuts at: 1707 3414 4886 1707
Size: 1707 1472 3528
Fragments arranged by size:
3528 1707 1472
NspI r_CATG'y
Cuts at: 3034 3760 4260 5167 3034
Size: 726 500 907 4574
Fragments arranged by size:
4574 907 726 500
PasI CC'CwG_GG
Cuts at: 335 1320 1773 2280 2895 3135 3162 3544 335
Size: 985 453 507 615 240 27 382 3498
Fragments arranged by size:
3498 985 615 507 453 382 240 27
PciI A'CATG_T
Cuts at: 3030 5163 3030
Size: 2133 4574
PflMI CCAn_nnn'nTGG
Cuts at: 341 341
Size: 6707
PfoI T'CCnGG_A
Cuts at: 777 1637 1805 1907 2341 777
Size: 860 168 102 434 5143
Fragments arranged by size:
5143 860 434 168 102
PmeI GTTT'AAAC
Cuts at: 3706 3706
Size: 6707
PmlI CAC'GTG
Cuts at: 1473 1473
Size: 6707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5846 5878 5846
Size: 32 6675
PpuMI rG'GwC_Cy
Cuts at: 218 793 845 1274 1356 1799 2012 2284 2684
Size: 575 52 429 82 443 213 272 400
Cuts at: 2684 2890 3067 218
Size: 206 177 3858
Fragments arranged by size:
3858 575 443 429 400 272 213 206
177 82 52
PshAI GACnn'nnGTC
Cuts at: 722 722
Size: 6707
PspOMI G'GGCC_C
Cuts at: 178 360 736 1455 1581 2838 3257 178
Size: 182 376 719 126 1257 419 3628
Fragments arranged by size:
3628 1257 719 419 376 182 126
PspXI vC'TCGA_Gb
Cuts at: 2087 2087
Size: 6707
PstI C_TGCA'G
Cuts at: 1040 1253 1381 1532 1881 1990 2795 2848 3005
Size: 213 128 151 349 109 805 53 157
Cuts at: 3005 3530 3536 3754 4439 1040
Size: 525 6 218 685 3308
Fragments arranged by size:
3308 805 685 525 349 218 213 157
151 128 109 53 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6707
PvuII CAG'CTG
Cuts at: 226 255 579 1114 1748 3406 3525 3531 4132
Size: 29 324 535 634 1658 119 6 601
Cuts at: 4132 4492 226
Size: 360 2441
Fragments arranged by size:
2441 1658 634 601 535 360 324 119
29 6
RsrII CG'GwC_CG
Cuts at: 4902 4902
Size: 6707
SacI G_AGCT'C
Cuts at: 1814 1858 2126 3721 1814
Size: 44 268 1595 4800
Fragments arranged by size:
4800 1595 268 44
SacII CC_GC'GG
Cuts at: 161 1574 161
Size: 1413 5294
SalI G'TCGA_C
Cuts at: 3744 3744
Size: 6707
SanDI GG'GwC_CC
Cuts at: 1356 2284 1356
Size: 928 5779
SapI GCTCTTCn'nnn_
Cuts at: 3157 4730 4940 3157
Size: 1573 210 4924
Fragments arranged by size:
4924 1573 210
SbfI CC_TGCA'GG
Cuts at: 3754 3754
Size: 6707
ScaI AGT'ACT
Cuts at: 661 6224 661
Size: 5563 1144
SexAI A'CCwGG_T
Cuts at: 3374 3374
Size: 6707
SfcI C'TryA_G
Cuts at: 33 1036 1249 1377 1528 1877 1986 2791 2844
Size: 1003 213 128 151 349 109 805 53
Cuts at: 2844 3001 3204 3526 3532 3750 4435 5428 5619
Size: 157 203 322 6 218 685 993 191
Cuts at: 5619 33
Size: 1121
Fragments arranged by size:
1121 1003 993 805 685 349 322 218
213 203 191 157 151 128 109 53
6
SfoI GGC'GCC
Cuts at: 1784 2546 3586 4139 4387 1784
Size: 762 1040 553 248 4104
Fragments arranged by size:
4104 1040 762 553 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6707
SmaI CCC'GGG
Cuts at: 1354 3729 1354
Size: 2375 4332
SmlI C'TyrA_G
Cuts at: 2087 3862 4777 5269 5531 2087
Size: 1775 915 492 262 3263
Fragments arranged by size:
3263 1775 915 492 262
SnaBI TAC'GTA
Cuts at: 3699 3699
Size: 6707
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6707
SphI G_CATG'C
Cuts at: 3760 4260 3760
Size: 500 6207
StuI AGG'CCT
Cuts at: 1738 2085 1738
Size: 347 6360
StyI C'CwwG_G
Cuts at: 125 174 380 765 832 2046 2128 2158 3624
Size: 49 206 385 67 1214 82 30 1466
Cuts at: 3624 3841 4195 125
Size: 217 354 2637
Fragments arranged by size:
2637 1466 1214 385 354 217 206 82
67 49 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 931 1834 2008 2614 2753 3448 4290 4423 4882
Size: 903 174 606 139 695 842 133 459
Cuts at: 4882 5091 931
Size: 209 2547
Fragments arranged by size:
2547 903 842 695 606 459 209 174
139 133
TatI w'GTAC_w
Cuts at: 348 659 6222 348
Size: 311 5563 833
Fragments arranged by size:
5563 833 311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1853 1885 1916 1948 1983 2015 2086 2118 3017
Size: 32 31 32 35 32 71 32 899
Cuts at: 3017 3049 6224 6256 1853
Size: 32 3175 32 2304
Fragments arranged by size:
3175 2304 899 71 35 32 32 32
32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 3012 4504 3012
Size: 1492 5215
XbaI T'CTAG_A
Cuts at: 3738 3738
Size: 6707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 695 1822 2296 2504 2902 3372 3403 4202 695
Size: 1127 474 208 398 470 31 799 3200
Fragments arranged by size:
3200 1127 799 474 470 398 208 31
XhoI C'TCGA_G
Cuts at: 2087 2087
Size: 6707
XmaI C'CCGG_G
Cuts at: 1352 3727 1352
Size: 2375 4332
ZraI GAC'GTC
Cuts at: 3245 3245
Size: 6707
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AflII AleI AloI AscI BaeI BbsI BfrBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PsiI PsrI SfiI SgrAI SrfI
SspI SwaI XmnI