Restriction map for
pF1KSDA1617
(Circular) (Six-base) MAPSORT of: pF1KSDA1617.seq Check: 9419 from: 1 to: 5819
pF1KSDA1617 5819 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2857 2857
Size: 5819
Acc65I G'GTAC_C
Cuts at: 2835 2835
Size: 5819
AclI AA'CG_TT
Cuts at: 5659 5659
Size: 5819
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 691 878 1945 2752 3644 4076 4823 5091
Size: 446 187 1067 807 892 432 747 268
Cuts at: 5091 245
Size: 973
Fragments arranged by size:
1067 973 892 807 747 446 432 268
187
AflIII A'CryG_T
Cuts at: 318 392 3171 4275 5252 318
Size: 74 2779 1104 977 885
Fragments arranged by size:
2779 1104 977 885 74
AgeI A'CCGG_T
Cuts at: 4207 4207
Size: 5819
AhdI GACnn_n'nnGTC
Cuts at: 2367 2367
Size: 5819
AleI CACnn'nnGTG
Cuts at: 2608 2608
Size: 5819
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1822 2016 1822
Size: 194 5625
AlwNI CAG_nnn'CTG
Cuts at: 435 1630 3124 4691 435
Size: 1195 1494 1567 1563
Fragments arranged by size:
1567 1563 1494 1195
ApaLI G'TGCA_C
Cuts at: 525 4589 525
Size: 4064 1755
ApoI r'AATT_y
Cuts at: 592 967 1905 1995 2037 2823 5172 5428 592
Size: 375 938 90 42 786 2349 256 983
Fragments arranged by size:
2349 983 938 786 375 256 90 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 5819
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5819
AvaI C'yCGr_G
Cuts at: 2268 2527 2839 2268
Size: 259 312 5248
Fragments arranged by size:
5248 312 259
BamHI G'GATC_C
Cuts at: 2844 2844
Size: 5819
BanI G'GyrC_C
Cuts at: 1208 2379 2475 2835 3249 3497 3532 1208
Size: 1171 96 360 414 248 35 3495
Fragments arranged by size:
3495 1171 414 360 248 96 35
BanII G_rGCy'C
Cuts at: 1067 2269 2345 2432 2833 3863 1067
Size: 1202 76 87 401 1030 3023
Fragments arranged by size:
3023 1202 1030 401 87 76
BbeI G_GCGC'C
Cuts at: 2479 3253 3501 2479
Size: 774 248 4797
Fragments arranged by size:
4797 774 248
BbsI GAAGACnn'nnnn_
Cuts at: 2234 2234
Size: 5819
BbvCI CC'TCA_GC
Cuts at: 1712 2490 1712
Size: 778 5041
BciVI GTATCCnnnnn_n'
Cuts at: 3717 4478 5819 3717
Size: 761 1341 3717
Fragments arranged by size:
3717 1341 761
BclI T'GATC_A
Cuts at: 3339 3339
Size: 5819
BglI GCCn_nnn'nGGC
Cuts at: 2647 2647
Size: 5819
BglII A'GATC_T
Cuts at: 2135 3334 5371 2135
Size: 1199 2037 2583
Fragments arranged by size:
2583 2037 1199
BlpI GC'TnA_GC
Cuts at: 195 2931 195
Size: 2736 3083
Bme1580I G_kGCm'C
Cuts at: 529 2384 2515 2643 3444 3537 4593 529
Size: 1855 131 128 801 93 1056 1755
Fragments arranged by size:
1855 1755 1056 801 131 128 93
BmgBI CAC'GTC
Cuts at: 2610 2610
Size: 5819
BmrI ACTGGGnnnn_n'
Cuts at: 270 408 1278 1507 2790 3031 3112 3447 5591
Size: 138 870 229 1283 241 81 335 2144
Cuts at: 5591 270
Size: 498
Fragments arranged by size:
2144 1283 870 498 335 241 229 138
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1246 2100 2352 2400 4186 1246
Size: 854 252 48 1786 2879
Fragments arranged by size:
2879 1786 854 252 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2596 2596
Size: 5819
Bpu10I CC'TnA_GC
Cuts at: 1712 2490 1712
Size: 778 5041
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 1063 2995 3874 4366 4664 219
Size: 844 1932 879 492 298 1374
Fragments arranged by size:
1932 1374 879 844 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 206 2323 5523 206
Size: 2117 3200 502
Fragments arranged by size:
3200 2117 502
BsaAI yAC'GTr
Cuts at: 395 422 604 2811 3172 395
Size: 27 182 2207 361 3042
Fragments arranged by size:
3042 2207 361 182 27
BsaBI GATnn'nnATC
Cuts at: 6 3333 5370 6
Size: 3327 2037 455
Fragments arranged by size:
3327 2037 455
BsaHI Gr'CG_yC
Cuts at: 2300 2476 3250 3498 4200 5696 2300
Size: 176 774 248 702 1496 2423
Fragments arranged by size:
2423 1496 774 702 248 176
BsaWI w'CCGG_w
Cuts at: 3008 3529 4207 4481 4628 5039 3008
Size: 521 678 274 147 411 3788
Fragments arranged by size:
3788 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1226 2302 2341 2478 2569 2572 2575 1226
Size: 1076 39 137 91 3 3 4470
Fragments arranged by size:
4470 1076 137 91 39 3 3
BseYI C'CCAG_C
Cuts at: 2736 3244 4002 4579 5075 2736
Size: 508 758 577 496 3480
Fragments arranged by size:
3480 758 577 508 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1535 2245 2734 1535
Size: 710 489 4620
Fragments arranged by size:
4620 710 489
BsiEI CG_ry'CG
Cuts at: 104 3361 3407 4615 104
Size: 3257 46 1208 1308
Fragments arranged by size:
3257 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 136 529 2345 2833 3611 3801 4593 136
Size: 393 1816 488 778 190 792 1362
Fragments arranged by size:
1816 1362 792 778 488 393 190
BsiWI C'GTAC_G
Cuts at: 1971 1971
Size: 5819
BsmI GAATG_Cn'
Cuts at: 332 332
Size: 5819
BsmBI CGTCTCn'nnnn_
Cuts at: 1912 1912
Size: 5819
Bsp1286I G_dGCh'C
Cuts at: 136 529 1067 2269 2345 2384 2432 2515 2643
Size: 393 538 1202 76 39 48 83 128
Cuts at: 2643 2833 3444 3537 3611 3801 3863 4593 136
Size: 190 611 93 74 190 62 730 1362
Fragments arranged by size:
1362 1202 730 611 538 393 190 190
128 93 83 76 74 62 48 39
BspEI T'CCGG_A
Cuts at: 5039 5039
Size: 5819
BspHI T'CATG_A
Cuts at: 1647 4995 1647
Size: 3348 2471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2869 3083 3385 3766 2869
Size: 214 302 381 4922
Fragments arranged by size:
4922 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2420 4111 4165 5649 5814 50
Size: 2370 1691 54 1484 165 55
Fragments arranged by size:
2370 1691 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3731 3731
Size: 5819
BsrFI r'CCGG_y
Cuts at: 2332 2438 2478 3817 3998 4207 2332
Size: 106 40 1339 181 209 3944
Fragments arranged by size:
3944 1339 209 181 106 40
BssSI C'ACGA_G
Cuts at: 4090 4448 4090
Size: 358 5461
BstAPI GCAn_nnn'nTGC
Cuts at: 295 435 3196 295
Size: 140 2761 2918
Fragments arranged by size:
2918 2761 140
BstBI TT'CG_AA
Cuts at: 110 4180 110
Size: 4070 1749
BstEII G'GTnAC_C
Cuts at: 2601 2601
Size: 5819
BstXI CCAn_nnnn'nTGG
Cuts at: 642 857 1205 1520 642
Size: 215 348 315 4941
Fragments arranged by size:
4941 348 315 215
BstYI r'GATC_y
Cuts at: 1 1893 2135 2844 3311 3334 3669 3915 4927
Size: 1892 242 709 467 23 335 246 1012
Cuts at: 4927 5013 5025 5371 1
Size: 86 12 346 449
Fragments arranged by size:
1892 1012 709 467 449 346 335 246
242 86 23 12
BtgI C'CryG_G
Cuts at: 125 515 1499 2249 2261 2303 2469 125
Size: 390 984 750 12 42 166 3475
Fragments arranged by size:
3475 984 750 390 166 42 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 483 3769 3950 4113 483
Size: 3286 181 163 2189
Fragments arranged by size:
3286 2189 181 163
BtsI GCAGTG_nn'
Cuts at: 3062 3062
Size: 5819
DraI TTT'AAA
Cuts at: 2818 2818
Size: 5819
DraIII CAC_nnn'GTG
Cuts at: 516 516
Size: 5819
DrdI GACnn_nn'nnGTC
Cuts at: 3525 4383 3525
Size: 858 4961
EaeI y'GGCC_r
Cuts at: 404 1254 2440 2648 2679 3404 3578 3969 3996
Size: 850 1186 208 31 725 174 391 27
Cuts at: 3996 5341 404
Size: 1345 882
Fragments arranged by size:
1345 1186 882 850 725 391 208 174
31 27
EagI C'GGCC_G
Cuts at: 3404 3404
Size: 5819
EarI CTCTTCn'nnn_
Cuts at: 272 980 1573 1723 1910 2279 3842 4052 272
Size: 708 593 150 187 369 1563 210 2039
Fragments arranged by size:
2039 1563 708 593 369 210 187 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2211 2389 4027 4337 4483 5629 2211
Size: 178 1638 310 146 1146 2401
Fragments arranged by size:
2401 1638 1146 310 178 146
EcoICRI GAG'CTC
Cuts at: 2343 2831 2343
Size: 488 5331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 691 878 1945 2596 2752 3644 4076 4823
Size: 446 187 1067 651 156 892 432 747
Cuts at: 4823 5091 245
Size: 268 973
Fragments arranged by size:
1067 973 892 747 651 446 432 268
187 156
EcoNI CCTnn'n_nnAGG
Cuts at: 656 1241 656
Size: 585 5234
EcoO109I rG'GnC_Cy
Cuts at: 649 667 1744 2740 2958 649
Size: 18 1077 996 218 3510
Fragments arranged by size:
3510 1077 996 218 18
EcoRI G'AATT_C
Cuts at: 2823 2823
Size: 5819
EcoRV GAT'ATC
Cuts at: 2060 2060
Size: 5819
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1843 3838 1843
Size: 1995 3824
FspI TGC'GCA
Cuts at: 3600 3600
Size: 5819
HaeII r_GCGC'y
Cuts at: 2479 3253 3501 4523 5502 2479
Size: 774 248 1022 979 2796
Fragments arranged by size:
2796 1022 979 774 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 790 822 1480 1512 2353 2385 3661
Size: 32 607 32 658 32 841 32 1276
Cuts at: 3661 3691 3693 3723 151
Size: 30 2 30 2247
Fragments arranged by size:
2247 1276 841 658 607 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1692 2858 1692
Size: 1166 4653
HindIII A'AGCT_T
Cuts at: 237 1039 237
Size: 802 5017
KasI G'GCGC_C
Cuts at: 2475 3249 3497 2475
Size: 774 248 4797
Fragments arranged by size:
4797 774 248
KpnI G_GTAC'C
Cuts at: 2839 2839
Size: 5819
MfeI C'AATT_G
Cuts at: 1612 1612
Size: 5819
MluI A'CGCG_T
Cuts at: 5252 5252
Size: 5819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 741 2570 2731 4490 4674 741
Size: 1829 161 1759 184 1886
Fragments arranged by size:
1886 1829 1759 184 161
MscI TGG'CCA
Cuts at: 406 1256 2650 3580 406
Size: 850 1394 930 2645
Fragments arranged by size:
2645 1394 930 850
MslI CAynn'nnrTG
Cuts at: 2221 2608 3808 3935 2221
Size: 387 1200 127 4105
Fragments arranged by size:
4105 1200 387 127
MspA1I CmG'CkG
Cuts at: 432 1884 2174 2263 2471 2930 3244 3604 4617
Size: 1452 290 89 208 459 314 360 1013
Cuts at: 4617 4862 432
Size: 245 1389
Fragments arranged by size:
1452 1389 1013 459 360 314 290 245
208 89
NaeI GCC'GGC
Cuts at: 2440 2480 4000 2440
Size: 40 1520 4259
Fragments arranged by size:
4259 1520 40
NarI GG'CG_CC
Cuts at: 2476 3250 3498 2476
Size: 774 248 4797
Fragments arranged by size:
4797 774 248
NcoI C'CATG_G
Cuts at: 125 1499 2303 125
Size: 1374 804 3641
Fragments arranged by size:
3641 1374 804
NgoMIV G'CCGG_C
Cuts at: 2438 2478 3998 2438
Size: 40 1520 4259
Fragments arranged by size:
4259 1520 40
NspI r_CATG'y
Cuts at: 2872 3372 4279 2872
Size: 500 907 4412
Fragments arranged by size:
4412 907 500
PciI A'CATG_T
Cuts at: 4275 4275
Size: 5819
PfoI T'CCnGG_A
Cuts at: 641 1389 1910 2345 641
Size: 748 521 435 4115
Fragments arranged by size:
4115 748 521 435
PmeI GTTT'AAAC
Cuts at: 2818 2818
Size: 5819
PmlI CAC'GTG
Cuts at: 422 422
Size: 5819
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4958 4990 4958
Size: 32 5787
PpuMI rG'GwC_Cy
Cuts at: 649 649
Size: 5819
PstI C_TGCA'G
Cuts at: 1463 2866 3551 1463
Size: 1403 685 3731
Fragments arranged by size:
3731 1403 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5819
PvuII CAG'CTG
Cuts at: 432 1884 3244 3604 432
Size: 1452 1360 360 2647
Fragments arranged by size:
2647 1452 1360 360
RsrII CG'GwC_CG
Cuts at: 4014 4014
Size: 5819
SacI G_AGCT'C
Cuts at: 2345 2833 2345
Size: 488 5331
SacII CC_GC'GG
Cuts at: 2264 2472 2264
Size: 208 5611
SalI G'TCGA_C
Cuts at: 2856 2856
Size: 5819
SapI GCTCTTCn'nnn_
Cuts at: 1723 3842 4052 1723
Size: 2119 210 3490
Fragments arranged by size:
3490 2119 210
SbfI CC_TGCA'GG
Cuts at: 2866 2866
Size: 5819
ScaI AGT'ACT
Cuts at: 733 5336 733
Size: 4603 1216
SfcI C'TryA_G
Cuts at: 33 671 1459 2862 3547 4540 4731 33
Size: 638 788 1403 685 993 191 1121
Fragments arranged by size:
1403 1121 993 788 685 638 191
SfoI GGC'GCC
Cuts at: 2477 3251 3499 2477
Size: 774 248 4797
Fragments arranged by size:
4797 774 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5819
SmaI CCC'GGG
Cuts at: 2841 2841
Size: 5819
SmlI C'TyrA_G
Cuts at: 234 1042 2974 3889 4381 4643 234
Size: 808 1932 915 492 262 1410
Fragments arranged by size:
1932 1410 915 808 492 262
SnaBI TAC'GTA
Cuts at: 2811 2811
Size: 5819
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5819
SphI G_CATG'C
Cuts at: 2872 3372 2872
Size: 500 5319
SspI AAT'ATT
Cuts at: 271 271
Size: 5819
StyI C'CwwG_G
Cuts at: 125 176 1499 2303 2645 2953 3307 125
Size: 51 1323 804 342 308 354 2637
Fragments arranged by size:
2637 1323 804 354 342 308 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 390 3402 3535 3994 4203 390
Size: 3012 133 459 209 2006
Fragments arranged by size:
3012 2006 459 209 133
TatI w'GTAC_w
Cuts at: 575 731 934 1200 5334 575
Size: 156 203 266 4134 1060
Fragments arranged by size:
4134 1060 266 203 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 729 761 5336 5368 729
Size: 32 4575 32 1180
Fragments arranged by size:
4575 1180 32 32
Tth111I GACn'n_nGTC
Cuts at: 493 1987 3616 493
Size: 1494 1629 2696
Fragments arranged by size:
2696 1629 1494
XbaI T'CTAG_A
Cuts at: 2850 2850
Size: 5819
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3314 3314
Size: 5819
XmaI C'CCGG_G
Cuts at: 2839 2839
Size: 5819
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BsaXI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI FseI FspAI HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI XmnI ZraI