Restriction map for
pF1KSDA1663
(Circular) (Six-base) MAPSORT of: pF1KSDA1663.seq Check: 6916 from: 1 to: 4169
pF1KSDA1663 4169 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1012 1012
Size: 4169
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 257 1185 257
Size: 928 3241
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1994 2426 3173 3441 829
Size: 1165 432 747 268 1557
Fragments arranged by size:
1557 1165 747 432 268
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 1474 3041 1474
Size: 1567 2602
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 679 1173 3522 3778 679
Size: 494 2349 256 1070
Fragments arranged by size:
2349 1070 494 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 1189 1189
Size: 4169
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 191 257 487 967 1000 1048 1185 1599 1847
Size: 66 230 480 33 48 137 414 248
Cuts at: 1847 1882 191
Size: 35 2478
Fragments arranged by size:
2478 480 414 248 230 137 66 48
35 33
BanII G_rGCy'C
Cuts at: 1183 2213 1183
Size: 1030 3139
BbeI G_GCGC'C
Cuts at: 195 1603 1851 195
Size: 1408 248 2513
Fragments arranged by size:
2513 1408 248
BbsI GAAGACnn'nnnn_
Cuts at: 1078 1078
Size: 4169
BbvCI CC'TCA_GC
Cuts at: 1093 1093
Size: 4169
BciVI GTATCCnnnnn_n'
Cuts at: 1130 2067 2828 4169 1130
Size: 937 761 1341 1130
Fragments arranged by size:
1341 1130 937 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BfrBI ATG'CAT
Cuts at: 837 837
Size: 4169
BglI GCCn_nnn'nGGC
Cuts at: 865 865
Size: 4169
BglII A'GATC_T
Cuts at: 1684 3721 1684
Size: 2037 2132
BlpI GC'TnA_GC
Cuts at: 638 1281 638
Size: 643 3526
Bme1580I G_kGCm'C
Cuts at: 492 551 1794 1887 2943 492
Size: 59 1243 93 1056 1718
Fragments arranged by size:
1718 1243 1056 93 59
BmrI ACTGGGnnnn_n'
Cuts at: 1381 1462 1797 3941 1381
Size: 81 335 2144 1609
Fragments arranged by size:
2144 1609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 842 2536 842
Size: 1694 2475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1087
Size: 4169
Bpu10I CC'TnA_GC
Cuts at: 1093 1093
Size: 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 2224 2716 3014 1345
Size: 879 492 298 2500
Fragments arranged by size:
2500 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3873 3873
Size: 4169
BsaAI yAC'GTr
Cuts at: 1161 1522 1161
Size: 361 3808
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 192 533 1600 1848 2550 4046 192
Size: 341 1067 248 702 1496 315
Fragments arranged by size:
1496 1067 702 341 315 248
BsaWI w'CCGG_w
Cuts at: 1358 1879 2557 2831 2978 3389 1358
Size: 521 678 274 147 411 2138
Fragments arranged by size:
2138 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 892 922 892
Size: 30 4139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 403 229
Size: 174 3995
BseYI C'CCAG_C
Cuts at: 610 878 928 1123 1594 2352 2929 3425 610
Size: 268 50 195 471 758 577 496 1354
Fragments arranged by size:
1354 758 577 496 471 268 195 50
BsiEI CG_ry'CG
Cuts at: 104 950 1711 1757 2965 104
Size: 846 761 46 1208 1308
Fragments arranged by size:
1308 1208 846 761 46
BsiHKAI G_wGCw'C
Cuts at: 1183 1961 2151 2943 1183
Size: 778 190 792 2409
Fragments arranged by size:
2409 792 778 190
BsmI GAATG_Cn'
Cuts at: 837 837
Size: 4169
Bsp1286I G_dGCh'C
Cuts at: 492 551 1183 1794 1887 1961 2151 2213 2943
Size: 59 632 611 93 74 190 62 730
Cuts at: 2943 492
Size: 1718
Fragments arranged by size:
1718 730 632 611 190 93 74 62
59
BspEI T'CCGG_A
Cuts at: 3389 3389
Size: 4169
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1012 1219 1433 1735 2116 1012
Size: 207 214 302 381 3065
Fragments arranged by size:
3065 381 302 214 207
BsrBI CCG'CTC
Cuts at: 50 225 632 637 788 2461 2515 3999 4164
Size: 175 407 5 151 1673 54 1484 165
Cuts at: 4164 50
Size: 55
Fragments arranged by size:
1673 1484 407 175 165 151 55 54
5
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 2167 2348 2557 2167
Size: 181 209 3779
Fragments arranged by size:
3779 209 181
BsrGI T'GTAC_A
Cuts at: 173 908 173
Size: 735 3434
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1546
Size: 4169
BstBI TT'CG_AA
Cuts at: 110 2530 110
Size: 2420 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1039 1039
Size: 4169
BstYI r'GATC_y
Cuts at: 1 1194 1661 1684 2019 2265 3277 3363 3375
Size: 1193 467 23 335 246 1012 86 12
Cuts at: 3375 3721 1
Size: 346 449
Fragments arranged by size:
1193 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 386 386
Size: 4169
BtgI C'CryG_G
Cuts at: 323 1142 323
Size: 819 3350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 572 2119 2300 2463 572
Size: 1547 181 163 2278
Fragments arranged by size:
2278 1547 181 163
BtsI GCAGTG_nn'
Cuts at: 747 996 1412 747
Size: 249 416 3504
Fragments arranged by size:
3504 416 249
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 330 365 663 698 900 935 330
Size: 35 298 35 202 35 3564
Fragments arranged by size:
3564 298 202 35 35 35
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 335 600 753 857 947 954 1151 1754 1928
Size: 265 153 104 90 7 197 603 174
Cuts at: 1928 2319 2346 3691 335
Size: 391 27 1345 813
Fragments arranged by size:
1345 813 603 391 265 197 174 153
104 90 27 7
EagI C'GGCC_G
Cuts at: 947 1754 947
Size: 807 3362
EarI CTCTTCn'nnn_
Cuts at: 940 1066 2192 2402 940
Size: 126 1126 210 2707
Fragments arranged by size:
2707 1126 210 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1087 1994 2426 3173 3441 829
Size: 258 907 432 747 268 1557
Fragments arranged by size:
1557 907 747 432 268 258
EcoO109I rG'GnC_Cy
Cuts at: 1308 1308
Size: 4169
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 537 1095 2188 537
Size: 558 1093 2518
Fragments arranged by size:
2518 1093 558
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 195 1603 1851 2873 3852 195
Size: 1408 248 1022 979 512
Fragments arranged by size:
1408 1022 979 512 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2011 2041 2043 2073 2011
Size: 30 2 30 4107
Fragments arranged by size:
4107 30 30 2
HincII GTy'rAC
Cuts at: 1208 1208
Size: 4169
KasI G'GCGC_C
Cuts at: 191 1599 1847 191
Size: 1408 248 2513
Fragments arranged by size:
2513 1408 248
KpnI G_GTAC'C
Cuts at: 261 1189 261
Size: 928 3241
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 602 2840 3024 602
Size: 2238 184 1747
Fragments arranged by size:
2238 1747 184
MscI TGG'CCA
Cuts at: 602 755 956 1153 1930 602
Size: 153 201 197 777 2841
Fragments arranged by size:
2841 777 201 197 153
MslI CAynn'nnrTG
Cuts at: 694 2158 2285 694
Size: 1464 127 2578
Fragments arranged by size:
2578 1464 127
MspA1I CmG'CkG
Cuts at: 293 965 1280 1594 1954 2967 3212 293
Size: 672 315 314 360 1013 245 1250
Fragments arranged by size:
1250 1013 672 360 315 314 245
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 192 1600 1848 192
Size: 1408 248 2513
Fragments arranged by size:
2513 1408 248
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NsiI A_TGCA'T
Cuts at: 839 839
Size: 4169
NspI r_CATG'y
Cuts at: 1222 1722 2629 1222
Size: 500 907 2762
Fragments arranged by size:
2762 907 500
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PflMI CCAn_nnn'nTGG
Cuts at: 598 1143 598
Size: 545 3624
PfoI T'CCnGG_A
Cuts at: 1064 1064
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PstI C_TGCA'G
Cuts at: 1216 1901 1216
Size: 685 3484
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 1594 1954 1594
Size: 360 3809
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 1212 1897 2890 3081 33
Size: 1179 685 993 191 1121
Fragments arranged by size:
1179 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 865 865
Size: 4169
SfoI GGC'GCC
Cuts at: 193 1601 1849 193
Size: 1408 248 2513
Fragments arranged by size:
2513 1408 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 1324 2239 2731 2993 1324
Size: 915 492 262 2500
Fragments arranged by size:
2500 915 492 262
SnaBI TAC'GTA
Cuts at: 1161 1161
Size: 4169
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
StuI AGG'CCT
Cuts at: 1091 1091
Size: 4169
StyI C'CwwG_G
Cuts at: 896 1303 1657 896
Size: 407 354 3408
Fragments arranged by size:
3408 407 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 624 807 903 942 1752 1885 2344 2553 624
Size: 183 96 39 810 133 459 209 2240
Fragments arranged by size:
2240 810 459 209 183 133 96 39
TatI w'GTAC_w
Cuts at: 173 908 3684 173
Size: 735 2776 658
Fragments arranged by size:
2776 735 658
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1664 1664
Size: 4169
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BmgBI BmtI BsgI BsiWI
BsmBI BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SrfI
SspI SwaI XhoI XmnI ZraI