Restriction map for
pF1KSDA1665
(Circular) (Six-base) MAPSORT of: pF1KSDA1665.seq Check: 1125 from: 1 to: 3749
pF1KSDA1665 3749 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 489 489
Size: 3749
AccI GT'mk_AC
Cuts at: 787 787
Size: 3749
Acc65I G'GTAC_C
Cuts at: 765 765
Size: 3749
AclI AA'CG_TT
Cuts at: 3589 3589
Size: 3749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2006 2753 3021 1574
Size: 432 747 268 2302
Fragments arranged by size:
2302 747 432 268
AflIII A'CryG_T
Cuts at: 602 1101 2205 3182 602
Size: 499 1104 977 1169
Fragments arranged by size:
1169 1104 977 499
AgeI A'CCGG_T
Cuts at: 2137 2137
Size: 3749
AleI CACnn'nnGTG
Cuts at: 682 682
Size: 3749
AlwNI CAG_nnn'CTG
Cuts at: 1054 2621 1054
Size: 1567 2182
ApaI G_GGCC'C
Cuts at: 620 620
Size: 3749
ApaLI G'TGCA_C
Cuts at: 415 2519 415
Size: 2104 1645
ApoI r'AATT_y
Cuts at: 753 3102 3358 753
Size: 2349 256 1144
Fragments arranged by size:
2349 1144 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3749
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
AvaI C'yCGr_G
Cuts at: 769 769
Size: 3749
BamHI G'GATC_C
Cuts at: 161 688 774 161
Size: 527 86 3136
Fragments arranged by size:
3136 527 86
BanI G'GyrC_C
Cuts at: 765 1179 1427 1462 765
Size: 414 248 35 3052
Fragments arranged by size:
3052 414 248 35
BanII G_rGCy'C
Cuts at: 620 763 1793 620
Size: 143 1030 2576
Fragments arranged by size:
2576 1030 143
BbeI G_GCGC'C
Cuts at: 1183 1431 1183
Size: 248 3501
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 184 218 184
Size: 34 3715
BciVI GTATCCnnnnn_n'
Cuts at: 1647 2408 3749 1647
Size: 761 1341 1647
Fragments arranged by size:
1647 1341 761
BclI T'GATC_A
Cuts at: 1269 1269
Size: 3749
BglII A'GATC_T
Cuts at: 1264 3301 1264
Size: 2037 1712
BlpI GC'TnA_GC
Cuts at: 624 861 624
Size: 237 3512
Bme1580I G_kGCm'C
Cuts at: 419 620 1374 1467 2523 419
Size: 201 754 93 1056 1645
Fragments arranged by size:
1645 1056 754 201 93
BmgBI CAC'GTC
Cuts at: 420 605 420
Size: 185 3564
BmrI ACTGGGnnnn_n'
Cuts at: 961 1042 1377 3521 961
Size: 81 335 2144 1189
Fragments arranged by size:
2144 1189 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 3749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 3749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1804 2296 2594 925
Size: 879 492 298 2080
Fragments arranged by size:
2080 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3453 3453
Size: 3749
BsaAI yAC'GTr
Cuts at: 741 1102 741
Size: 361 3388
BsaBI GATnn'nnATC
Cuts at: 6 1263 3300 6
Size: 1257 2037 455
Fragments arranged by size:
2037 1257 455
BsaHI Gr'CG_yC
Cuts at: 1180 1428 2130 3626 1180
Size: 248 702 1496 1303
Fragments arranged by size:
1496 1303 702 248
BsaWI w'CCGG_w
Cuts at: 158 938 1459 2137 2411 2558 2969 158
Size: 780 521 678 274 147 411 938
Fragments arranged by size:
938 780 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 404 434 487 517 658 688 404
Size: 30 53 30 141 30 3465
Fragments arranged by size:
3465 141 53 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 577 664 223
Size: 354 87 3308
Fragments arranged by size:
3308 354 87
BseYI C'CCAG_C
Cuts at: 619 1174 1932 2509 3005 619
Size: 555 758 577 496 1363
Fragments arranged by size:
1363 758 577 555 496
BsiEI CG_ry'CG
Cuts at: 104 1291 1337 2545 104
Size: 1187 46 1208 1308
Fragments arranged by size:
1308 1208 1187 46
BsiHKAI G_wGCw'C
Cuts at: 419 537 763 1541 1731 2523 419
Size: 118 226 778 190 792 1645
Fragments arranged by size:
1645 792 778 226 190 118
BsmBI CGTCTCn'nnnn_
Cuts at: 426 514 426
Size: 88 3661
Bsp1286I G_dGCh'C
Cuts at: 419 537 620 763 1374 1467 1541 1731 1793
Size: 118 83 143 611 93 74 190 62
Cuts at: 1793 2523 419
Size: 730 1645
Fragments arranged by size:
1645 730 611 190 143 118 93 83
74 62
BspEI T'CCGG_A
Cuts at: 158 2969 158
Size: 2811 938
BspHI T'CATG_A
Cuts at: 2925 2925
Size: 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 489 799 1013 1315 1696 489
Size: 310 214 302 381 2542
Fragments arranged by size:
2542 381 310 302 214
BsrBI CCG'CTC
Cuts at: 50 2041 2095 3579 3744 50
Size: 1991 54 1484 165 55
Fragments arranged by size:
1991 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1661 1661
Size: 3749
BsrFI r'CCGG_y
Cuts at: 556 1747 1928 2137 556
Size: 1191 181 209 2168
Fragments arranged by size:
2168 1191 209 181
BsrGI T'GTAC_A
Cuts at: 242 242
Size: 3749
BssSI C'ACGA_G
Cuts at: 2020 2378 2020
Size: 358 3391
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 1126
Size: 3749
BstBI TT'CG_AA
Cuts at: 110 2110 110
Size: 2000 1749
BstYI r'GATC_y
Cuts at: 1 161 566 688 774 1241 1264 1599 1845
Size: 160 405 122 86 467 23 335 246
Cuts at: 1845 2857 2943 2955 3301 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 405 346 335 246 160
122 86 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1699 1880 2043 1699
Size: 181 163 3405
Fragments arranged by size:
3405 181 163
BtsI GCAGTG_nn'
Cuts at: 463 992 463
Size: 529 3220
DraI TTT'AAA
Cuts at: 748 748
Size: 3749
DrdI GACnn_nn'nnGTC
Cuts at: 1455 2313 1455
Size: 858 2891
EaeI y'GGCC_r
Cuts at: 227 1334 1508 1899 1926 3271 227
Size: 1107 174 391 27 1345 705
Fragments arranged by size:
1345 1107 705 391 174 27
EagI C'GGCC_G
Cuts at: 1334 1334
Size: 3749
EarI CTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1957 2267 2413 3559 1957
Size: 310 146 1146 2147
Fragments arranged by size:
2147 1146 310 146
EcoICRI GAG'CTC
Cuts at: 761 761
Size: 3749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 1574 2006 2753 3021 307
Size: 1267 432 747 268 1035
Fragments arranged by size:
1267 1035 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 616 888 616
Size: 272 3477
EcoRI G'AATT_C
Cuts at: 753 753
Size: 3749
EcoRV GAT'ATC
Cuts at: 276 276
Size: 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1768 1768
Size: 3749
FspI TGC'GCA
Cuts at: 1530 1530
Size: 3749
HaeII r_GCGC'y
Cuts at: 1183 1431 2453 3432 1183
Size: 248 1022 979 1500
Fragments arranged by size:
1500 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 304 336 404 436 1591 1621 1623 1653 304
Size: 32 68 32 1155 30 2 30 2400
Fragments arranged by size:
2400 1155 68 32 32 30 30 2
HincII GTy'rAC
Cuts at: 600 788 600
Size: 188 3561
KasI G'GCGC_C
Cuts at: 1179 1427 1179
Size: 248 3501
KpnI G_GTAC'C
Cuts at: 769 769
Size: 3749
MluI A'CGCG_T
Cuts at: 3182 3182
Size: 3749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2420 2604 2420
Size: 184 3565
MscI TGG'CCA
Cuts at: 229 1510 229
Size: 1281 2468
MslI CAynn'nnrTG
Cuts at: 682 1738 1865 682
Size: 1056 127 2566
Fragments arranged by size:
2566 1056 127
MspA1I CmG'CkG
Cuts at: 860 1174 1534 2547 2792 860
Size: 314 360 1013 245 1817
Fragments arranged by size:
1817 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1930 1930
Size: 3749
NarI GG'CG_CC
Cuts at: 1180 1428 1180
Size: 248 3501
NgoMIV G'CCGG_C
Cuts at: 1928 1928
Size: 3749
NspI r_CATG'y
Cuts at: 802 1302 2209 802
Size: 500 907 2342
Fragments arranged by size:
2342 907 500
PasI CC'CwG_GG
Cuts at: 307 307
Size: 3749
PciI A'CATG_T
Cuts at: 2205 2205
Size: 3749
PmeI GTTT'AAAC
Cuts at: 748 748
Size: 3749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2888 2920 2888
Size: 32 3717
PspOMI G'GGCC_C
Cuts at: 616 616
Size: 3749
PstI C_TGCA'G
Cuts at: 403 470 796 1481 403
Size: 67 326 685 2671
Fragments arranged by size:
2671 685 326 67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3749
PvuII CAG'CTG
Cuts at: 1174 1534 1174
Size: 360 3389
RsrII CG'GwC_CG
Cuts at: 1944 1944
Size: 3749
SacI G_AGCT'C
Cuts at: 763 763
Size: 3749
SalI G'TCGA_C
Cuts at: 786 786
Size: 3749
SapI GCTCTTCn'nnn_
Cuts at: 1772 1982 1772
Size: 210 3539
SbfI CC_TGCA'GG
Cuts at: 796 796
Size: 3749
ScaI AGT'ACT
Cuts at: 3266 3266
Size: 3749
SfcI C'TryA_G
Cuts at: 33 399 466 792 1477 2470 2661 33
Size: 366 67 326 685 993 191 1121
Fragments arranged by size:
1121 993 685 366 326 191 67
SfoI GGC'GCC
Cuts at: 1181 1429 1181
Size: 248 3501
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3749
SgrAI Cr'CCGG_yG
Cuts at: 556 556
Size: 3749
SmaI CCC'GGG
Cuts at: 771 771
Size: 3749
SmlI C'TyrA_G
Cuts at: 904 1819 2311 2573 904
Size: 915 492 262 2080
Fragments arranged by size:
2080 915 492 262
SnaBI TAC'GTA
Cuts at: 741 741
Size: 3749
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3749
SphI G_CATG'C
Cuts at: 802 1302 802
Size: 500 3249
StuI AGG'CCT
Cuts at: 707 707
Size: 3749
StyI C'CwwG_G
Cuts at: 169 883 1237 169
Size: 714 354 2681
Fragments arranged by size:
2681 714 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 495 1332 1465 1924 2133 495
Size: 837 133 459 209 2111
Fragments arranged by size:
2111 837 459 209 133
TatI w'GTAC_w
Cuts at: 242 3264 242
Size: 3022 727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 487 519 3266 3298 487
Size: 32 2747 32 938
Fragments arranged by size:
2747 938 32 32
Tth111I GACn'n_nGTC
Cuts at: 154 1546 154
Size: 1392 2357
XbaI T'CTAG_A
Cuts at: 780 780
Size: 3749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1244 132
Size: 1112 2637
XmaI C'CCGG_G
Cuts at: 769 769
Size: 3749
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I BsgI
BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SrfI SspI SwaI XhoI
XmnI ZraI