Restriction map for
pF1KSDA1674
(Circular) (Six-base) MAPSORT of: pF1KSDA1674.seq Check: 9127 from: 1 to: 4727
pF1KSDA1674 4727 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1605 1765 1605
Size: 160 4567
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2552 2984 3731 3999 2552
Size: 432 747 268 3280
Fragments arranged by size:
3280 747 432 268
AfeI AGC'GCT
Cuts at: 440 622 440
Size: 182 4545
AflIII A'CryG_T
Cuts at: 749 1041 2079 3183 4160 749
Size: 292 1038 1104 977 1316
Fragments arranged by size:
1316 1104 1038 977 292
AgeI A'CCGG_T
Cuts at: 687 3115 687
Size: 2428 2299
AhdI GACnn_n'nnGTC
Cuts at: 750 1092 750
Size: 342 4385
AleI CACnn'nnGTG
Cuts at: 236 236
Size: 4727
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 371 1291 371
Size: 920 3807
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 638 670 638
Size: 32 4695
AlwNI CAG_nnn'CTG
Cuts at: 1179 2032 3599 1179
Size: 853 1567 2307
Fragments arranged by size:
2307 1567 853
ApaLI G'TGCA_C
Cuts at: 3497 3497
Size: 4727
ApoI r'AATT_y
Cuts at: 574 1431 1731 4080 4336 574
Size: 857 300 2349 256 965
Fragments arranged by size:
2349 965 857 300 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 717 1747 717
Size: 1030 3697
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 1743 2157 2405 2440 1743
Size: 414 248 35 4030
Fragments arranged by size:
4030 414 248 35
BanII G_rGCy'C
Cuts at: 462 724 1163 1236 1265 1741 2771 462
Size: 262 439 73 29 476 1030 2418
Fragments arranged by size:
2418 1030 476 439 262 73 29
BbeI G_GCGC'C
Cuts at: 2161 2409 2161
Size: 248 4479
BbsI GAAGACnn'nnnn_
Cuts at: 1063 1063
Size: 4727
BbvCI CC'TCA_GC
Cuts at: 584 919 584
Size: 335 4392
BciVI GTATCCnnnnn_n'
Cuts at: 2625 3386 4727 2625
Size: 761 1341 2625
Fragments arranged by size:
2625 1341 761
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglII A'GATC_T
Cuts at: 1323 2242 4279 1323
Size: 919 2037 1771
Fragments arranged by size:
2037 1771 919
BlpI GC'TnA_GC
Cuts at: 1264 1839 1264
Size: 575 4152
Bme1580I G_kGCm'C
Cuts at: 1212 1419 2352 2445 3501 1212
Size: 207 933 93 1056 2438
Fragments arranged by size:
2438 1056 933 207 93
BmgBI CAC'GTC
Cuts at: 1458 1458
Size: 4727
BmrI ACTGGGnnnn_n'
Cuts at: 1939 2020 2355 4499 1939
Size: 81 335 2144 2167
Fragments arranged by size:
2167 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 985 3094 985
Size: 2109 2618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 751 761 1053 1147 1525 196
Size: 555 10 292 94 378 3398
Fragments arranged by size:
3398 555 378 292 94 10
Bpu10I CC'TnA_GC
Cuts at: 584 776 919 584
Size: 192 143 4392
Fragments arranged by size:
4392 192 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 359 659 982 1903 2782 3274 3572 300
Size: 59 300 323 921 879 492 298 1455
Fragments arranged by size:
1455 921 879 492 323 300 298 59
BsaI GGTCTCn'nnnn_
Cuts at: 737 4431 737
Size: 3694 1033
BsaAI yAC'GTr
Cuts at: 942 1719 2080 942
Size: 777 361 3589
Fragments arranged by size:
3589 777 361
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 2158 2406 3108 4604 2158
Size: 248 702 1496 2281
Fragments arranged by size:
2281 1496 702 248
BsaWI w'CCGG_w
Cuts at: 419 687 1916 2437 3115 3389 3536 3947 419
Size: 268 1229 521 678 274 147 411 1199
Fragments arranged by size:
1229 1199 678 521 411 274 268 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 638 668 638
Size: 30 4697
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 252 322 572 861 907 910 1056 1302
Size: 59 70 250 289 46 3 146 246
Cuts at: 1302 1522 193
Size: 220 3398
Fragments arranged by size:
3398 289 250 246 220 146 70 59
46 3
BseYI C'CCAG_C
Cuts at: 325 522 886 2152 2910 3487 3983 325
Size: 197 364 1266 758 577 496 1069
Fragments arranged by size:
1266 1069 758 577 496 364 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 616
Size: 4727
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3523 104
Size: 2165 46 1208 1308
Fragments arranged by size:
2165 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 462 941 1163 1265 1741 2519 2709 3501 462
Size: 479 222 102 476 778 190 792 1688
Fragments arranged by size:
1688 792 778 479 476 222 190 102
BsmI GAATG_Cn'
Cuts at: 360 360
Size: 4727
BsmBI CGTCTCn'nnnn_
Cuts at: 1188 1188
Size: 4727
Bsp1286I G_dGCh'C
Cuts at: 462 724 941 1163 1212 1236 1265 1419 1741
Size: 262 217 222 49 24 29 154 322
Cuts at: 1741 2352 2445 2519 2709 2771 3501 462
Size: 611 93 74 190 62 730 1688
Fragments arranged by size:
1688 730 611 322 262 222 217 190
154 93 74 62 49 29 24
BspEI T'CCGG_A
Cuts at: 3947 3947
Size: 4727
BspHI T'CATG_A
Cuts at: 3903 3903
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1777 1991 2293 2674 1777
Size: 214 302 381 3830
Fragments arranged by size:
3830 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1144 3019 3073 4557 4722 50
Size: 1094 1875 54 1484 165 55
Fragments arranged by size:
1875 1484 1094 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2639 2639
Size: 4727
BsrFI r'CCGG_y
Cuts at: 687 1644 2725 2906 3115 687
Size: 957 1081 181 209 2299
Fragments arranged by size:
2299 1081 957 209 181
BssSI C'ACGA_G
Cuts at: 2998 3356 2998
Size: 358 4369
BstAPI GCAn_nnn'nTGC
Cuts at: 2104 2104
Size: 4727
BstBI TT'CG_AA
Cuts at: 110 3088 110
Size: 2978 1749
BstYI r'GATC_y
Cuts at: 1 1323 1530 1752 2219 2242 2577 2823 3835
Size: 1322 207 222 467 23 335 246 1012
Cuts at: 3835 3921 3933 4279 1
Size: 86 12 346 449
Fragments arranged by size:
1322 1012 467 449 346 335 246 222
207 86 23 12
BtgI C'CryG_G
Cuts at: 125 1282 1488 125
Size: 1157 206 3364
Fragments arranged by size:
3364 1157 206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 561 1210 1970 561
Size: 649 760 3318
Fragments arranged by size:
3318 760 649
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3291 2433
Size: 858 3869
EaeI y'GGCC_r
Cuts at: 1491 2312 2486 2877 2904 4249 1491
Size: 821 174 391 27 1345 1969
Fragments arranged by size:
1969 1345 821 391 174 27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 1127 1243 1253 1433 2750 2960 1127
Size: 116 10 180 1317 210 2894
Fragments arranged by size:
2894 1317 210 180 116 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1346 2935 3245 3391 4537 1346
Size: 1589 310 146 1146 1536
Fragments arranged by size:
1589 1536 1146 310 146
EcoICRI GAG'CTC
Cuts at: 460 1161 1263 1739 460
Size: 701 102 476 3448
Fragments arranged by size:
3448 701 476 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 751 761 1053 1147 1525 2552 2984 3731
Size: 555 10 292 94 378 1027 432 747
Cuts at: 3731 3999 196
Size: 268 924
Fragments arranged by size:
1027 924 747 555 432 378 292 268
94 10
EcoNI CCTnn'n_nnAGG
Cuts at: 1068 1068
Size: 4727
EcoO109I rG'GnC_Cy
Cuts at: 780 1497 1866 780
Size: 717 369 3641
Fragments arranged by size:
3641 717 369
EcoRI G'AATT_C
Cuts at: 574 1731 574
Size: 1157 3570
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 831 1023 2746 831
Size: 192 1723 2812
Fragments arranged by size:
2812 1723 192
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 442 624 909 1153 2161 2409 3431 4410 442
Size: 182 285 244 1008 248 1022 979 759
Fragments arranged by size:
1022 1008 979 759 285 248 244 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 236 268 444 476 736 768 1078 1110 2569
Size: 32 176 32 260 32 310 32 1459
Cuts at: 2569 2599 2601 2631 236
Size: 30 2 30 2332
Fragments arranged by size:
2332 1459 310 260 176 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1606 1766 1606
Size: 160 4567
KasI G'GCGC_C
Cuts at: 2157 2405 2157
Size: 248 4479
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MfeI C'AATT_G
Cuts at: 337 337
Size: 4727
MluI A'CGCG_T
Cuts at: 749 4160 749
Size: 3411 1316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 222 691 3398 3582 222
Size: 469 2707 184 1367
Fragments arranged by size:
2707 1367 469 184
MscI TGG'CCA
Cuts at: 1493 2488 1493
Size: 995 3732
MslI CAynn'nnrTG
Cuts at: 236 2716 2843 236
Size: 2480 127 2120
Fragments arranged by size:
2480 2120 127
MspA1I CmG'CkG
Cuts at: 1284 1838 2152 2512 3525 3770 1284
Size: 554 314 360 1013 245 2241
Fragments arranged by size:
2241 1013 554 360 314 245
NaeI GCC'GGC
Cuts at: 1646 2908 1646
Size: 1262 3465
NarI GG'CG_CC
Cuts at: 2158 2406 2158
Size: 248 4479
NcoI C'CATG_G
Cuts at: 125 1488 125
Size: 1363 3364
NgoMIV G'CCGG_C
Cuts at: 1644 2906 1644
Size: 1262 3465
NspI r_CATG'y
Cuts at: 1780 2280 3187 1780
Size: 500 907 3320
Fragments arranged by size:
3320 907 500
PciI A'CATG_T
Cuts at: 3183 3183
Size: 4727
PfoI T'CCnGG_A
Cuts at: 1069 1163 1069
Size: 94 4633
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PmlI CAC'GTG
Cuts at: 942 942
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 638 670 3866 3898 638
Size: 32 3196 32 1467
Fragments arranged by size:
3196 1467 32 32
PpuMI rG'GwC_Cy
Cuts at: 780 780
Size: 4727
PstI C_TGCA'G
Cuts at: 1217 1424 1774 2459 1217
Size: 207 350 685 3485
Fragments arranged by size:
3485 685 350 207
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 2152 2512 2152
Size: 360 4367
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 462 1163 1265 1741 462
Size: 701 102 476 3448
Fragments arranged by size:
3448 701 476 102
SacII CC_GC'GG
Cuts at: 1285 1285
Size: 4727
SalI G'TCGA_C
Cuts at: 1604 1764 1604
Size: 160 4567
SapI GCTCTTCn'nnn_
Cuts at: 1253 2750 2960 1253
Size: 1497 210 3020
Fragments arranged by size:
3020 1497 210
SbfI CC_TGCA'GG
Cuts at: 1424 1774 1424
Size: 350 4377
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SfcI C'TryA_G
Cuts at: 33 1213 1420 1552 1770 2455 3448 3639 33
Size: 1180 207 132 218 685 993 191 1121
Fragments arranged by size:
1180 1121 993 685 218 207 191 132
SfoI GGC'GCC
Cuts at: 2159 2407 2159
Size: 248 4479
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1749 1749
Size: 4727
SmlI C'TyrA_G
Cuts at: 279 374 674 997 1882 2797 3289 3551 279
Size: 95 300 323 885 915 492 262 1455
Fragments arranged by size:
1455 915 885 492 323 300 262 95
SnaBI TAC'GTA
Cuts at: 1719 1719
Size: 4727
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1780 2280 1780
Size: 500 4227
StyI C'CwwG_G
Cuts at: 125 759 1488 1861 2215 125
Size: 634 729 373 354 2637
Fragments arranged by size:
2637 729 634 373 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2310 2443 2902 3111 2310
Size: 133 459 209 3926
Fragments arranged by size:
3926 459 209 133
TatI w'GTAC_w
Cuts at: 4242 4242
Size: 4727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 4695
Tth111I GACn'n_nGTC
Cuts at: 2524 2524
Size: 4727
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2222
Size: 4727
XmaI C'CCGG_G
Cuts at: 1747 1747
Size: 4727
XmnI GAAnn'nnTTC
Cuts at: 1629 1629
Size: 4727
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII ApaI AscI AvrII BaeI BcgI
BfrBI BglI BmtI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
ZraI