Restriction map for
pF1KSDA1686
(Circular) (Six-base) MAPSORT of: pF1KSDA1686.seq Check: 4895 from: 1 to: 6661
pF1KSDA1686 6661 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 3697 3697
Size: 6661
Acc65I G'GTAC_C
Cuts at: 3675 3675
Size: 6661
AclI AA'CG_TT
Cuts at: 6501 6501
Size: 6661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 503 824 1511 2210 2251 2792 3071 3258
Size: 24 321 687 699 41 541 279 187
Cuts at: 3258 3479 4484 4916 5665 5933 479
Size: 221 1005 432 749 268 1207
Fragments arranged by size:
1207 1005 749 699 687 541 432 321
279 268 221 187 41 24
AflIII A'CryG_T
Cuts at: 2410 4011 5117 6094 2410
Size: 1601 1106 977 2977
Fragments arranged by size:
2977 1601 1106 977
AgeI A'CCGG_T
Cuts at: 630 5049 630
Size: 4419 2242
AhdI GACnn_n'nnGTC
Cuts at: 1908 1908
Size: 6661
AleI CACnn'nnGTG
Cuts at: 2528 2528
Size: 6661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 313 313
Size: 6661
AlwNI CAG_nnn'CTG
Cuts at: 943 2788 3964 5533 943
Size: 1845 1176 1569 2071
Fragments arranged by size:
2071 1845 1569 1176
ApaI G_GGCC'C
Cuts at: 1313 1875 1313
Size: 562 6099
ApaLI G'TGCA_C
Cuts at: 1216 2564 5431 1216
Size: 1348 2867 2446
Fragments arranged by size:
2867 2446 1348
ApoI r'AATT_y
Cuts at: 603 1164 1350 2096 2555 2591 3117 3345 3663
Size: 561 186 746 459 36 526 228 318
Cuts at: 3663 6014 6270 603
Size: 2351 256 994
Fragments arranged by size:
2351 994 746 561 526 459 318 256
228 186 36
AseI AT'TA_AT
Cuts at: 20 815 1148 20
Size: 795 333 5533
Fragments arranged by size:
5533 795 333
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6661
AvaI C'yCGr_G
Cuts at: 2426 3679 2426
Size: 1253 5408
BamHI G'GATC_C
Cuts at: 3684 3684
Size: 6661
BanI G'GyrC_C
Cuts at: 3675 4089 4337 4372 3675
Size: 414 248 35 5964
Fragments arranged by size:
5964 414 248 35
BanII G_rGCy'C
Cuts at: 547 1313 1470 1875 3673 4703 547
Size: 766 157 405 1798 1030 2505
Fragments arranged by size:
2505 1798 1030 766 405 157
BbeI G_GCGC'C
Cuts at: 4093 4341 4093
Size: 248 6413
BbsI GAAGACnn'nnnn_
Cuts at: 195 1817 3036 195
Size: 1622 1219 3820
Fragments arranged by size:
3820 1622 1219
BciVI GTATCCnnnnn_n'
Cuts at: 763 1783 2041 4557 5320 6661 763
Size: 1020 258 2516 763 1341 763
Fragments arranged by size:
2516 1341 1020 763 763 258
BclI T'GATC_A
Cuts at: 4179 4179
Size: 6661
BfrBI ATG'CAT
Cuts at: 2337 2337
Size: 6661
BglI GCCn_nnn'nGGC
Cuts at: 551 551
Size: 6661
BglII A'GATC_T
Cuts at: 1278 4174 6213 1278
Size: 2896 2039 1726
Fragments arranged by size:
2896 2039 1726
BlpI GC'TnA_GC
Cuts at: 3771 3771
Size: 6661
Bme1580I G_kGCm'C
Cuts at: 1220 1313 1875 2568 4284 4377 5435 1220
Size: 93 562 693 1716 93 1058 2446
Fragments arranged by size:
2446 1716 1058 693 562 93 93
BmrI ACTGGGnnnn_n'
Cuts at: 3871 3952 4287 6433 3871
Size: 81 335 2146 4099
Fragments arranged by size:
4099 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 171 5026 171
Size: 4855 1806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 166
Size: 6661
Bpu10I CC'TnA_GC
Cuts at: 1883 3329 1883
Size: 1446 5215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1257 2092 2317 2925 3398 3835 4714 5208 5506
Size: 835 225 608 473 437 879 494 298
Cuts at: 5506 1257
Size: 2412
Fragments arranged by size:
2412 879 835 608 494 473 437 298
225
BsaI GGTCTCn'nnnn_
Cuts at: 6365 6365
Size: 6661
BsaAI yAC'GTr
Cuts at: 2411 3651 4012 2411
Size: 1240 361 5060
Fragments arranged by size:
5060 1240 361
BsaBI GATnn'nnATC
Cuts at: 6 1381 4173 6212 6
Size: 1375 2792 2039 455
Fragments arranged by size:
2792 2039 1375 455
BsaHI Gr'CG_yC
Cuts at: 4090 4338 5042 6538 4090
Size: 248 704 1496 4213
Fragments arranged by size:
4213 1496 704 248
BsaWI w'CCGG_w
Cuts at: 271 630 3848 4369 5049 5323 5470 5881 271
Size: 359 3218 521 680 274 147 411 1051
Fragments arranged by size:
3218 1051 680 521 411 359 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2029 2059 2096 2126 2029
Size: 30 37 30 6564
Fragments arranged by size:
6564 37 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 1606 1856 1990 232
Size: 1374 250 134 4903
Fragments arranged by size:
4903 1374 250 134
BseYI C'CCAG_C
Cuts at: 308 2349 4084 4842 5421 5917 308
Size: 2041 1735 758 579 496 1052
Fragments arranged by size:
2041 1735 1052 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 855 2975 226
Size: 629 2120 3912
Fragments arranged by size:
3912 2120 629
BsiEI CG_ry'CG
Cuts at: 104 198 1332 4201 4247 5457 104
Size: 94 1134 2869 46 1210 1308
Fragments arranged by size:
2869 1308 1210 1134 94 46
BsiHKAI G_wGCw'C
Cuts at: 232 292 1220 1675 2568 3673 4451 4641 5435
Size: 60 928 455 893 1105 778 190 794
Cuts at: 5435 232
Size: 1458
Fragments arranged by size:
1458 1105 928 893 794 778 455 190
60
BsmBI CGTCTCn'nnnn_
Cuts at: 1998 1998
Size: 6661
Bsp1286I G_dGCh'C
Cuts at: 232 292 547 1220 1313 1470 1675 1875 2568
Size: 60 255 673 93 157 205 200 693
Cuts at: 2568 3673 4284 4377 4451 4641 4703 5435 232
Size: 1105 611 93 74 190 62 732 1458
Fragments arranged by size:
1458 1105 732 693 673 611 255 205
200 190 157 93 93 74 62 60
BspEI T'CCGG_A
Cuts at: 5881 5881
Size: 6661
BspHI T'CATG_A
Cuts at: 1401 1563 5837 1401
Size: 162 4274 2225
Fragments arranged by size:
4274 2225 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 382 3709 3923 4225 4606 382
Size: 3327 214 302 381 2437
Fragments arranged by size:
3327 2437 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1588 4951 5005 6491 6656 50
Size: 1538 3363 54 1486 165 55
Fragments arranged by size:
3363 1538 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4571 4571
Size: 6661
BsrFI r'CCGG_y
Cuts at: 253 278 630 4657 4838 5049 253
Size: 25 352 4027 181 211 1865
Fragments arranged by size:
4027 1865 352 211 181 25
BssSI C'ACGA_G
Cuts at: 563 4930 5290 563
Size: 4367 360 1934
Fragments arranged by size:
4367 1934 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1204 2307 4036 1204
Size: 1103 1729 3829
Fragments arranged by size:
3829 1729 1103
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6661
BstEII G'GTnAC_C
Cuts at: 267 267
Size: 6661
BstXI CCAn_nnnn'nTGG
Cuts at: 1713 2133 1713
Size: 420 6241
BstYI r'GATC_y
Cuts at: 1 135 152 1034 1278 2928 3684 4151 4174
Size: 134 17 882 244 1650 756 467 23
Cuts at: 4174 4509 4755 5769 5855 5867 6213 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1650 1014 882 756 467 449 346 335
246 244 134 86 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 3049 3392 3524 3049
Size: 343 132 6186
Fragments arranged by size:
6186 343 132
BtgI C'CryG_G
Cuts at: 125 1712 1764 125
Size: 1587 52 5022
Fragments arranged by size:
5022 1587 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3575 4609 4790 4953 3575
Size: 1034 181 163 5283
Fragments arranged by size:
5283 1034 181 163
BtsI GCAGTG_nn'
Cuts at: 536 1551 2432 2963 3179 3560 3902 536
Size: 1015 881 531 216 381 342 3295
Fragments arranged by size:
3295 1015 881 531 381 342 216
DraI TTT'AAA
Cuts at: 3658 3658
Size: 6661
DraIII CAC_nnn'GTG
Cuts at: 1625 1625
Size: 6661
DrdI GACnn_nn'nnGTC
Cuts at: 1935 4365 5225 1935
Size: 2430 860 3371
Fragments arranged by size:
3371 2430 860
EaeI y'GGCC_r
Cuts at: 195 475 4244 4418 4809 4836 6183 195
Size: 280 3769 174 391 27 1347 673
Fragments arranged by size:
3769 1347 673 391 280 174 27
EagI C'GGCC_G
Cuts at: 195 4244 195
Size: 4049 2612
EarI CTCTTCn'nnn_
Cuts at: 740 2252 3002 3443 4682 4892 740
Size: 1512 750 441 1239 210 2509
Fragments arranged by size:
2509 1512 1239 750 441 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 148 4867 5179 5325 6471 148
Size: 4719 312 146 1146 338
Fragments arranged by size:
4719 1146 338 312 146
EcoICRI GAG'CTC
Cuts at: 3671 3671
Size: 6661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 479 503 824 1511 2210 2251 2792 3071
Size: 313 24 321 687 699 41 541 279
Cuts at: 3071 3258 3479 4484 4916 5665 5933 166
Size: 187 221 1005 432 749 268 894
Fragments arranged by size:
1005 894 749 699 687 541 432 321
313 279 268 221 187 41 24
EcoO109I rG'GnC_Cy
Cuts at: 1309 1310 1579 1871 3798 1309
Size: 1 269 292 1927 4172
Fragments arranged by size:
4172 1927 292 269 1
EcoRI G'AATT_C
Cuts at: 1164 2555 2591 3663 1164
Size: 1391 36 1072 4162
Fragments arranged by size:
4162 1391 1072 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 4678 2281
Size: 2397 4264
FspI TGC'GCA
Cuts at: 4440 4440
Size: 6661
HaeII r_GCGC'y
Cuts at: 4093 4341 5365 6344 4093
Size: 248 1024 979 4410
Fragments arranged by size:
4410 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1177 1209 1894 1926 2913 2945 3420 3452 4501
Size: 32 685 32 987 32 475 32 1049
Cuts at: 4501 4531 4533 4563 1177
Size: 30 2 30 3275
Fragments arranged by size:
3275 1049 987 685 475 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2738 3483 3698 2738
Size: 745 215 5701
Fragments arranged by size:
5701 745 215
HindIII A'AGCT_T
Cuts at: 497 2068 2303 497
Size: 1571 235 4855
Fragments arranged by size:
4855 1571 235
HpaI GTT'AAC
Cuts at: 2738 2738
Size: 6661
KasI G'GCGC_C
Cuts at: 4089 4337 4089
Size: 248 6413
KpnI G_GTAC'C
Cuts at: 3679 3679
Size: 6661
MfeI C'AATT_G
Cuts at: 411 411
Size: 6661
MluI A'CGCG_T
Cuts at: 6094 6094
Size: 6661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1346 2817 5332 5516 1346
Size: 1471 2515 184 2491
Fragments arranged by size:
2515 2491 1471 184
MscI TGG'CCA
Cuts at: 4420 4420
Size: 6661
MslI CAynn'nnrTG
Cuts at: 1892 2528 3643 4648 4775 1892
Size: 636 1115 1005 127 3778
Fragments arranged by size:
3778 1115 1005 636 127
MspA1I CmG'CkG
Cuts at: 1921 2145 2517 3770 4084 4444 5459 5704 1921
Size: 224 372 1253 314 360 1015 245 2878
Fragments arranged by size:
2878 1253 1015 372 360 314 245 224
NaeI GCC'GGC
Cuts at: 4840 4840
Size: 6661
NarI GG'CG_CC
Cuts at: 4090 4338 4090
Size: 248 6413
NcoI C'CATG_G
Cuts at: 125 1712 125
Size: 1587 5074
NdeI CA'TA_TG
Cuts at: 3309 3309
Size: 6661
NgoMIV G'CCGG_C
Cuts at: 4838 4838
Size: 6661
NruI TCG'CGA
Cuts at: 5022 5022
Size: 6661
NsiI A_TGCA'T
Cuts at: 2339 2339
Size: 6661
NspI r_CATG'y
Cuts at: 855 1202 1891 2251 2341 3712 4212 5121 855
Size: 347 689 360 90 1371 500 909 2395
Fragments arranged by size:
2395 1371 909 689 500 360 347 90
PciI A'CATG_T
Cuts at: 5117 5117
Size: 6661
PflMI CCAn_nnn'nTGG
Cuts at: 1476 3267 1476
Size: 1791 4870
PfoI T'CCnGG_A
Cuts at: 169 2033 169
Size: 1864 4797
PmeI GTTT'AAAC
Cuts at: 3658 3658
Size: 6661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5800 5832 5800
Size: 32 6629
PpuMI rG'GwC_Cy
Cuts at: 1579 1579
Size: 6661
PsiI TTA'TAA
Cuts at: 656 1661 656
Size: 1005 5656
PspOMI G'GGCC_C
Cuts at: 1309 1871 1309
Size: 562 6099
PstI C_TGCA'G
Cuts at: 1546 3706 4391 1546
Size: 2160 685 3816
Fragments arranged by size:
3816 2160 685
PvuI CG_AT'CG
Cuts at: 104 1332 104
Size: 1228 5433
PvuII CAG'CTG
Cuts at: 1921 2145 2517 4084 4444 1921
Size: 224 372 1567 360 4138
Fragments arranged by size:
4138 1567 372 360 224
RsrII CG'GwC_CG
Cuts at: 4854 4854
Size: 6661
SacI G_AGCT'C
Cuts at: 3673 3673
Size: 6661
SalI G'TCGA_C
Cuts at: 3696 3696
Size: 6661
SapI GCTCTTCn'nnn_
Cuts at: 3002 3443 4682 4892 3002
Size: 441 1239 210 4771
Fragments arranged by size:
4771 1239 441 210
SbfI CC_TGCA'GG
Cuts at: 3706 3706
Size: 6661
ScaI AGT'ACT
Cuts at: 669 1692 2985 6178 669
Size: 1023 1293 3193 1152
Fragments arranged by size:
3193 1293 1152 1023
SfcI C'TryA_G
Cuts at: 33 548 1221 1542 2642 3702 4387 5382 5573
Size: 515 673 321 1100 1060 685 995 191
Cuts at: 5573 33
Size: 1121
Fragments arranged by size:
1121 1100 1060 995 685 673 515 321
191
SfoI GGC'GCC
Cuts at: 4091 4339 4091
Size: 248 6413
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6661
SgrAI Cr'CCGG_yG
Cuts at: 253 278 253
Size: 25 6636
SmaI CCC'GGG
Cuts at: 3681 3681
Size: 6661
SmlI C'TyrA_G
Cuts at: 1236 2071 2296 2426 2904 3413 3814 4729 5223
Size: 835 225 130 478 509 401 915 494
Cuts at: 5223 5485 1236
Size: 262 2412
Fragments arranged by size:
2412 915 835 509 494 478 401 262
225 130
SnaBI TAC'GTA
Cuts at: 3651 3651
Size: 6661
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6661
SphI G_CATG'C
Cuts at: 1202 2251 2341 3712 4212 1202
Size: 1049 90 1371 500 3651
Fragments arranged by size:
3651 1371 1049 500 90
SspI AAT'ATT
Cuts at: 1722 1722
Size: 6661
StuI AGG'CCT
Cuts at: 1530 1530
Size: 6661
StyI C'CwwG_G
Cuts at: 125 1712 1959 2526 2992 3793 4147 125
Size: 1587 247 567 466 801 354 2639
Fragments arranged by size:
2639 1587 801 567 466 354 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4242 4375 4834 5045 4242
Size: 133 459 211 5858
Fragments arranged by size:
5858 459 211 133
TatI w'GTAC_w
Cuts at: 667 930 1690 2687 2983 6176 667
Size: 263 760 997 296 3193 1152
Fragments arranged by size:
3193 1152 997 760 296 263
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2096 2128 6178 6210 2096
Size: 32 4050 32 2547
Fragments arranged by size:
4050 2547 32 32
Tth111I GACn'n_nGTC
Cuts at: 4456 4456
Size: 6661
XbaI T'CTAG_A
Cuts at: 3532 3690 3532
Size: 158 6503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2364 2439 2833 4154 2364
Size: 75 394 1321 4871
Fragments arranged by size:
4871 1321 394 75
XhoI C'TCGA_G
Cuts at: 2426 2426
Size: 6661
XmaI C'CCGG_G
Cuts at: 3679 3679
Size: 6661
XmnI GAAnn'nnTTC
Cuts at: 1350 3236 1350
Size: 1886 4775
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BaeI
BbvCI BcgI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI NheI
NotI PacI PasI PmlI PshAI PspXI PsrI SacII
SanDI SexAI SfiI SrfI SwaI ZraI