Restriction map for
pF1KSDA1688
(Circular) (Six-base) MAPSORT of: pF1KSDA1688.seq Check: 7008 from: 1 to: 6386
pF1KSDA1688 6386 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2952 2952
Size: 6386
AccI GT'mk_AC
Cuts at: 167 1322 3424 167
Size: 1155 2102 3129
Fragments arranged by size:
3129 2102 1155
Acc65I G'GTAC_C
Cuts at: 3032 3402 3032
Size: 370 6016
AclI AA'CG_TT
Cuts at: 6226 6226
Size: 6386
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 973 1917 2746 2901 2974 3043 4211 4643
Size: 501 944 829 155 73 69 1168 432
Cuts at: 4643 5390 5658 472
Size: 747 268 1200
Fragments arranged by size:
1200 1168 944 829 747 501 432 268
155 73 69
AfeI AGC'GCT
Cuts at: 1267 2830 1267
Size: 1563 4823
AflIII A'CryG_T
Cuts at: 353 3738 4842 5819 353
Size: 3385 1104 977 920
Fragments arranged by size:
3385 1104 977 920
AgeI A'CCGG_T
Cuts at: 268 4774 268
Size: 4506 1880
AhdI GACnn_n'nnGTC
Cuts at: 1569 1569
Size: 6386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2815 2815
Size: 6386
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 451 483 2203 2235 451
Size: 32 1720 32 4602
Fragments arranged by size:
4602 1720 32 32
AlwNI CAG_nnn'CTG
Cuts at: 450 1236 1424 1446 1689 1973 2016 2463 3345
Size: 786 188 22 243 284 43 447 882
Cuts at: 3345 3691 5258 450
Size: 346 1567 1578
Fragments arranged by size:
1578 1567 882 786 447 346 284 243
188 43 22
ApaI G_GGCC'C
Cuts at: 1216 1663 1216
Size: 447 5939
ApaLI G'TGCA_C
Cuts at: 3124 5156 3124
Size: 2032 4354
ApoI r'AATT_y
Cuts at: 3390 5739 5995 3390
Size: 2349 256 3781
Fragments arranged by size:
3781 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6386
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6386
AvaI C'yCGr_G
Cuts at: 496 550 744 1261 1590 1663 1912 2836 3103
Size: 54 194 517 329 73 249 924 267
Cuts at: 3103 3406 496
Size: 303 3476
Fragments arranged by size:
3476 924 517 329 303 267 249 194
73 54
BamHI G'GATC_C
Cuts at: 3411 3411
Size: 6386
BanI G'GyrC_C
Cuts at: 401 748 913 1891 1984 2979 3032 3249 3402
Size: 347 165 978 93 995 53 217 153
Cuts at: 3402 3816 4064 4099 401
Size: 414 248 35 2688
Fragments arranged by size:
2688 995 978 414 347 248 217 165
153 93 53 35
BanII G_rGCy'C
Cuts at: 551 557 791 968 1073 1109 1216 1342 1663
Size: 6 234 177 105 36 107 126 321
Cuts at: 1663 1781 2066 2363 2477 2546 3053 3400 4430
Size: 118 285 297 114 69 507 347 1030
Cuts at: 4430 551
Size: 2507
Fragments arranged by size:
2507 1030 507 347 321 297 285 234
177 126 118 114 107 105 69 36
6
BbeI G_GCGC'C
Cuts at: 752 1895 3253 3820 4068 752
Size: 1143 1358 567 248 3070
Fragments arranged by size:
3070 1358 1143 567 248
BbsI GAAGACnn'nnnn_
Cuts at: 2154 3002 3177 2154
Size: 848 175 5363
Fragments arranged by size:
5363 848 175
BbvCI CC'TCA_GC
Cuts at: 1432 1432
Size: 6386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2929 2963 2929
Size: 34 6352
BciVI GTATCCnnnnn_n'
Cuts at: 4284 5045 6386 4284
Size: 761 1341 4284
Fragments arranged by size:
4284 1341 761
BclI T'GATC_A
Cuts at: 3906 3906
Size: 6386
BglI GCCn_nnn'nGGC
Cuts at: 524 904 1759 1810 2138 524
Size: 380 855 51 328 4772
Fragments arranged by size:
4772 855 380 328 51
BglII A'GATC_T
Cuts at: 3901 5938 3901
Size: 2037 4349
BlpI GC'TnA_GC
Cuts at: 3498 3498
Size: 6386
Bme1580I G_kGCm'C
Cuts at: 1216 1597 1663 1987 2109 2984 3128 4011 4104
Size: 381 66 324 122 875 144 883 93
Cuts at: 4104 5160 1216
Size: 1056 2442
Fragments arranged by size:
2442 1056 883 875 381 324 144 122
93 66
BmgBI CAC'GTC
Cuts at: 356 1119 3006 356
Size: 763 1887 3736
Fragments arranged by size:
3736 1887 763
BmrI ACTGGGnnnn_n'
Cuts at: 1069 2103 2223 3598 3679 4014 6158 1069
Size: 1034 120 1375 81 335 2144 1297
Fragments arranged by size:
2144 1375 1297 1034 335 120 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4753 4753
Size: 6386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1180 1300 1326 1995 2041 2136 2310 2449
Size: 615 120 26 669 46 95 174 139
Cuts at: 2449 2469 2539 3064 565
Size: 20 70 525 3887
Fragments arranged by size:
3887 669 615 525 174 139 120 95
70 46 26 20
Bpu10I CC'TnA_GC
Cuts at: 1273 1432 1487 1273
Size: 159 55 6172
Fragments arranged by size:
6172 159 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 3562 4441 4933 5231 682
Size: 2880 879 492 298 1837
Fragments arranged by size:
2880 1837 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6090 6090
Size: 6386
BsaAI yAC'GTr
Cuts at: 1308 1326 2340 2421 2663 3378 3739 1308
Size: 18 1014 81 242 715 361 3955
Fragments arranged by size:
3955 1014 715 361 242 81 18
BsaBI GATnn'nnATC
Cuts at: 6 3900 5937 6
Size: 3894 2037 455
Fragments arranged by size:
3894 2037 455
BsaHI Gr'CG_yC
Cuts at: 299 749 1451 1892 3250 3817 4065 4767 6263
Size: 450 702 441 1358 567 248 702 1496
Cuts at: 6263 299
Size: 422
Fragments arranged by size:
1496 1358 702 702 567 450 441 422
248
BsaWI w'CCGG_w
Cuts at: 268 1459 3575 4096 4774 5048 5195 5606 268
Size: 1191 2116 521 678 274 147 411 1048
Fragments arranged by size:
2116 1191 1048 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 709 739 2203 2233 709
Size: 30 1464 30 4862
Fragments arranged by size:
4862 1464 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 528 717 948 1020 1081 1857 1992 2181 2758
Size: 189 231 72 61 776 135 189 577
Cuts at: 2758 2890 3061 528
Size: 132 171 3853
Fragments arranged by size:
3853 776 577 231 189 189 171 135
132 72 61
BseYI C'CCAG_C
Cuts at: 790 880 995 1160 1417 1850 1859 1955 2146
Size: 90 115 165 257 433 9 96 191
Cuts at: 2146 2537 3811 4569 5146 5642 790
Size: 391 1274 758 577 496 1534
Fragments arranged by size:
1534 1274 758 577 496 433 391 257
191 165 115 96 90 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 2059 2401 2476 2878 3211 171
Size: 1888 342 75 402 333 3346
Fragments arranged by size:
3346 1888 402 342 333 75
BsiEI CG_ry'CG
Cuts at: 104 296 409 1168 1189 1775 2334 3928 3974
Size: 192 113 759 21 586 559 1594 46
Cuts at: 3974 5182 104
Size: 1208 1308
Fragments arranged by size:
1594 1308 1208 759 586 559 192 113
46 21
BsiHKAI G_wGCw'C
Cuts at: 968 1244 2363 2477 2546 3128 3335 3400 4178
Size: 276 1119 114 69 582 207 65 778
Cuts at: 4178 4368 5160 968
Size: 190 792 2194
Fragments arranged by size:
2194 1119 792 778 582 276 207 190
114 69 65
BsmI GAATG_Cn'
Cuts at: 2952 2952
Size: 6386
BsmBI CGTCTCn'nnnn_
Cuts at: 2037 2191 2037
Size: 154 6232
Bsp1286I G_dGCh'C
Cuts at: 551 557 791 968 1073 1109 1216 1244 1342
Size: 6 234 177 105 36 107 28 98
Cuts at: 1342 1597 1663 1781 1987 2066 2109 2363 2477
Size: 255 66 118 206 79 43 254 114
Cuts at: 2477 2546 2984 3053 3128 3335 3400 4011 4104
Size: 69 438 69 75 207 65 611 93
Cuts at: 4104 4178 4368 4430 5160 551
Size: 74 190 62 730 1777
Fragments arranged by size:
1777 730 611 438 255 254 234 207
206 190 177 118 114 107 105 98
93 79 75 74 69 69 66 65
62 43 36 28 6
BspEI T'CCGG_A
Cuts at: 1459 5606 1459
Size: 4147 2239
BspHI T'CATG_A
Cuts at: 5562 5562
Size: 6386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 179 1679 2952 3171 3436 3650 3952 4333 179
Size: 1500 1273 219 265 214 302 381 2232
Fragments arranged by size:
2232 1500 1273 381 302 265 219 214
BsrBI CCG'CTC
Cuts at: 50 848 1059 1741 4678 4732 6216 6381 50
Size: 798 211 682 2937 54 1484 165 55
Fragments arranged by size:
2937 1484 798 682 211 165 55 54
BsrDI GCAATG_nn'
Cuts at: 819 4298 819
Size: 3479 2907
BsrFI r'CCGG_y
Cuts at: 268 291 295 404 1150 1294 1360 1917 2123
Size: 23 4 109 746 144 66 557 206
Cuts at: 2123 2333 2393 2492 2615 3195 4384 4565 4774
Size: 210 60 99 123 580 1189 181 209
Cuts at: 4774 268
Size: 1880
Fragments arranged by size:
1880 1189 746 580 557 210 209 206
181 144 123 109 99 66 60 23
4
BsrGI T'GTAC_A
Cuts at: 2381 2381
Size: 6386
BssHII G'CGCG_C
Cuts at: 474 1356 474
Size: 882 5504
BssSI C'ACGA_G
Cuts at: 3064 4657 5015 3064
Size: 1593 358 4435
Fragments arranged by size:
4435 1593 358
BstAPI GCAn_nnn'nTGC
Cuts at: 579 1239 2463 3132 3763 579
Size: 660 1224 669 631 3202
Fragments arranged by size:
3202 1224 669 660 631
BstBI TT'CG_AA
Cuts at: 110 4747 110
Size: 4637 1749
BstEII G'GTnAC_C
Cuts at: 264 2389 2896 3210 264
Size: 2125 507 314 3440
Fragments arranged by size:
3440 2125 507 314
BstXI CCAn_nnnn'nTGG
Cuts at: 814 2283 2559 2991 3246 3360 814
Size: 1469 276 432 255 114 3840
Fragments arranged by size:
3840 1469 432 276 255 114
BstYI r'GATC_y
Cuts at: 1 2012 2747 3411 3878 3901 4236 4482 5494
Size: 2011 735 664 467 23 335 246 1012
Cuts at: 5494 5580 5592 5938 1
Size: 86 12 346 449
Fragments arranged by size:
2011 1012 735 664 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 602 1112 1366 1475 2532 3119 3239 602
Size: 510 254 109 1057 587 120 3749
Fragments arranged by size:
3749 1057 587 510 254 120 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2256 3069 4336 4517 4680 2256
Size: 813 1267 181 163 3962
Fragments arranged by size:
3962 1267 813 181 163
BtsI GCAGTG_nn'
Cuts at: 1900 2413 2797 3085 3629 1900
Size: 513 384 288 544 4657
Fragments arranged by size:
4657 544 513 384 288
DraI TTT'AAA
Cuts at: 3385 3385
Size: 6386
DraIII CAC_nnn'GTG
Cuts at: 398 398
Size: 6386
DrdI GACnn_nn'nnGTC
Cuts at: 3004 3286 4092 4950 3004
Size: 282 806 858 4440
Fragments arranged by size:
4440 858 806 282
EaeI y'GGCC_r
Cuts at: 293 406 434 702 1186 1691 1772 2435 2549
Size: 113 28 268 484 505 81 663 114
Cuts at: 2549 3116 3197 3236 3971 4145 4536 4563 5908
Size: 567 81 39 735 174 391 27 1345
Cuts at: 5908 293
Size: 771
Fragments arranged by size:
1345 771 735 663 567 505 484 391
268 174 114 113 81 81 39 28
27
EagI C'GGCC_G
Cuts at: 293 406 1186 1772 3971 293
Size: 113 780 586 2199 2708
Fragments arranged by size:
2708 2199 780 586 113
EarI CTCTTCn'nnn_
Cuts at: 645 949 1603 2020 2257 2368 4409 4619 645
Size: 304 654 417 237 111 2041 210 2412
Fragments arranged by size:
2412 2041 654 417 304 237 210 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 499 2274 2503 4594 4904 5050 6196 499
Size: 1775 229 2091 310 146 1146 689
Fragments arranged by size:
2091 1775 1146 689 310 229 146
EcoICRI GAG'CTC
Cuts at: 966 2361 2475 2544 3398 966
Size: 1395 114 69 854 3954
Fragments arranged by size:
3954 1395 854 114 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 565 973 1180 1300 1326 1917 1995 2041
Size: 93 408 207 120 26 591 78 46
Cuts at: 2041 2136 2310 2449 2469 2539 2746 2901 2974
Size: 95 174 139 20 70 207 155 73
Cuts at: 2974 3043 3064 4211 4643 5390 5658 472
Size: 69 21 1147 432 747 268 1200
Fragments arranged by size:
1200 1147 747 591 432 408 268 207
207 174 155 139 120 95 93 78
73 70 69 46 26 21 20
EcoNI CCTnn'n_nnAGG
Cuts at: 664 1693 1789 664
Size: 1029 96 5261
Fragments arranged by size:
5261 1029 96
EcoO109I rG'GnC_Cy
Cuts at: 285 722 1089 1212 1213 1483 1659 1660 1668
Size: 437 367 123 1 270 176 1 8
Cuts at: 1668 2091 2247 2460 2922 3525 285
Size: 423 156 213 462 603 3146
Fragments arranged by size:
3146 603 462 437 423 367 270 213
176 156 123 8 1 1
EcoRI G'AATT_C
Cuts at: 3390 3390
Size: 6386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2019 4405 2019
Size: 2386 4000
FspI TGC'GCA
Cuts at: 2182 4167 2182
Size: 1985 4401
HaeII r_GCGC'y
Cuts at: 752 1269 1446 1646 1895 1951 2832 2844 2890
Size: 517 177 200 249 56 881 12 46
Cuts at: 2890 3253 3820 4068 5090 6069 752
Size: 363 567 248 1022 979 1069
Fragments arranged by size:
1069 1022 979 881 567 517 363 249
248 200 177 56 46 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1528 1555 1560 1587 4228 4258 4260 4290 1528
Size: 27 5 27 2641 30 2 30 3624
Fragments arranged by size:
3624 2641 30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 168 3425 168
Size: 3257 3129
KasI G'GCGC_C
Cuts at: 748 1891 3249 3816 4064 748
Size: 1143 1358 567 248 3070
Fragments arranged by size:
3070 1358 1143 567 248
KpnI G_GTAC'C
Cuts at: 3036 3406 3036
Size: 370 6016
MluI A'CGCG_T
Cuts at: 5819 5819
Size: 6386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 188 3299 5057 5241 188
Size: 3111 1758 184 1333
Fragments arranged by size:
3111 1758 1333 184
MscI TGG'CCA
Cuts at: 436 2437 2551 3238 4147 436
Size: 2001 114 687 909 2675
Fragments arranged by size:
2675 2001 909 687 114
MslI CAynn'nnrTG
Cuts at: 1117 2320 2815 3217 3244 4375 4502 1117
Size: 1203 495 402 27 1131 127 3001
Fragments arranged by size:
3001 1203 1131 495 402 127 27
MspA1I CmG'CkG
Cuts at: 432 604 632 987 1164 1236 1317 1368 1518
Size: 172 28 355 177 72 81 51 150
Cuts at: 1518 1850 2138 2156 2487 2534 2847 3497 3811
Size: 332 288 18 331 47 313 650 314
Cuts at: 3811 4171 5184 5429 432
Size: 360 1013 245 1389
Fragments arranged by size:
1389 1013 650 360 355 332 331 314
313 288 245 177 172 150 81 72
51 47 28 18
NaeI GCC'GGC
Cuts at: 293 297 1152 1296 1362 2125 2494 3197 4567
Size: 4 855 144 66 763 369 703 1370
Cuts at: 4567 293
Size: 2112
Fragments arranged by size:
2112 1370 855 763 703 369 144 66
4
NarI GG'CG_CC
Cuts at: 749 1892 3250 3817 4065 749
Size: 1143 1358 567 248 3070
Fragments arranged by size:
3070 1358 1143 567 248
NcoI C'CATG_G
Cuts at: 1112 1475 3239 1112
Size: 363 1764 4259
Fragments arranged by size:
4259 1764 363
NgoMIV G'CCGG_C
Cuts at: 291 295 1150 1294 1360 2123 2492 3195 4565
Size: 4 855 144 66 763 369 703 1370
Cuts at: 4565 291
Size: 2112
Fragments arranged by size:
2112 1370 855 763 703 369 144 66
4
NspI r_CATG'y
Cuts at: 252 765 2283 2758 2820 3439 3939 4846 252
Size: 513 1518 475 62 619 500 907 1792
Fragments arranged by size:
1792 1518 907 619 513 500 475 62
PasI CC'CwG_GG
Cuts at: 807 970 2852 2926 807
Size: 163 1882 74 4267
Fragments arranged by size:
4267 1882 163 74
PciI A'CATG_T
Cuts at: 4842 4842
Size: 6386
PflMI CCAn_nnn'nTGG
Cuts at: 1236 1446 1547 2447 3345 1236
Size: 210 101 900 898 4277
Fragments arranged by size:
4277 900 898 210 101
PmeI GTTT'AAAC
Cuts at: 3385 3385
Size: 6386
PmlI CAC'GTG
Cuts at: 2340 2421 2340
Size: 81 6305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1043 1075 2203 2235 5525 5557 1043
Size: 32 1128 32 3290 32 1872
Fragments arranged by size:
3290 1872 1128 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 285 722 1483 1668 2091 2922 285
Size: 437 761 185 423 831 3749
Fragments arranged by size:
3749 831 761 437 423 185
PspOMI G'GGCC_C
Cuts at: 1212 1659 1212
Size: 447 5939
PstI C_TGCA'G
Cuts at: 446 890 2804 2963 3182 3433 4118 446
Size: 444 1914 159 219 251 685 2714
Fragments arranged by size:
2714 1914 685 444 251 219 159
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6386
PvuII CAG'CTG
Cuts at: 1236 1317 1850 2156 2487 2847 3811 4171 1236
Size: 81 533 306 331 360 964 360 3451
Fragments arranged by size:
3451 964 533 360 360 331 306 81
RsrII CG'GwC_CG
Cuts at: 4581 4581
Size: 6386
SacI G_AGCT'C
Cuts at: 968 2363 2477 2546 3400 968
Size: 1395 114 69 854 3954
Fragments arranged by size:
3954 1395 854 114 69
SacII CC_GC'GG
Cuts at: 605 1369 2535 605
Size: 764 1166 4456
Fragments arranged by size:
4456 1166 764
SalI G'TCGA_C
Cuts at: 166 3423 166
Size: 3257 3129
SanDI GG'GwC_CC
Cuts at: 285 2922 285
Size: 2637 3749
SapI GCTCTTCn'nnn_
Cuts at: 645 1603 2020 2368 4409 4619 645
Size: 958 417 348 2041 210 2412
Fragments arranged by size:
2412 2041 958 417 348 210
SbfI CC_TGCA'GG
Cuts at: 3182 3433 3182
Size: 251 6135
ScaI AGT'ACT
Cuts at: 2674 5903 2674
Size: 3229 3157
SexAI A'CCwGG_T
Cuts at: 260 260
Size: 6386
SfcI C'TryA_G
Cuts at: 33 442 717 886 1467 2689 2800 2959 3178
Size: 409 275 169 581 1222 111 159 219
Cuts at: 3178 3429 4114 5107 5298 33
Size: 251 685 993 191 1121
Fragments arranged by size:
1222 1121 993 685 581 409 275 251
219 191 169 159 111
SfoI GGC'GCC
Cuts at: 750 1893 3251 3818 4066 750
Size: 1143 1358 567 248 3070
Fragments arranged by size:
3070 1358 1143 567 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6386
SgrAI Cr'CCGG_yG
Cuts at: 268 268
Size: 6386
SmaI CCC'GGG
Cuts at: 498 1592 1665 1914 3408 498
Size: 1094 73 249 1494 3476
Fragments arranged by size:
3476 1494 1094 249 73
SmlI C'TyrA_G
Cuts at: 661 3541 4456 4948 5210 661
Size: 2880 915 492 262 1837
Fragments arranged by size:
2880 1837 915 492 262
SnaBI TAC'GTA
Cuts at: 3378 3378
Size: 6386
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6386
SphI G_CATG'C
Cuts at: 765 2758 2820 3439 3939 765
Size: 1993 62 619 500 3212
Fragments arranged by size:
3212 1993 619 500 62
SrfI GCCC'GGGC
Cuts at: 1914 1914
Size: 6386
StuI AGG'CCT
Cuts at: 2353 2990 2353
Size: 637 5749
StyI C'CwwG_G
Cuts at: 1112 1475 2402 2441 3239 3520 3874 1112
Size: 363 927 39 798 281 354 3624
Fragments arranged by size:
3624 927 798 363 354 281 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 204 894 2377 2844 3969 4102 4561 4770 204
Size: 690 1483 467 1125 133 459 209 1820
Fragments arranged by size:
1820 1483 1125 690 467 459 209 133
TatI w'GTAC_w
Cuts at: 1283 2381 2672 5901 1283
Size: 1098 291 3229 1768
Fragments arranged by size:
3229 1768 1098 291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 133 165 827 859 5903 5935 133
Size: 32 662 32 5044 32 584
Fragments arranged by size:
5044 662 584 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2629 4183 2629
Size: 1554 4832
XbaI T'CTAG_A
Cuts at: 3417 3417
Size: 6386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 540 3881 540
Size: 3341 3045
XmaI C'CCGG_G
Cuts at: 496 1590 1663 1912 3406 496
Size: 1094 73 249 1494 3476
Fragments arranged by size:
3476 1494 1094 249 73
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AscI AvrII BaeI BfrBI BmtI
BsiWI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PshAI PsiI PspXI PsrI SfiI SspI
SwaI XhoI XmnI ZraI