Restriction map for
pF1KSDA1701
(Circular) (Six-base) MAPSORT of: pF1KSDA1701.seq Check: 2347 from: 1 to: 4778
pF1KSDA1701 4778 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 1017 1017
Size: 4778
AccI GT'mk_AC
Cuts at: 1816 1816
Size: 4778
Acc65I G'GTAC_C
Cuts at: 1794 1794
Size: 4778
AclI AA'CG_TT
Cuts at: 4618 4618
Size: 4778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 2603 3035 3782 4050 902
Size: 1701 432 747 268 1630
Fragments arranged by size:
1701 1630 747 432 268
AflII C'TTAA_G
Cuts at: 1120 1120
Size: 4778
AflIII A'CryG_T
Cuts at: 424 1335 2130 3234 4211 424
Size: 911 795 1104 977 991
Fragments arranged by size:
1104 991 977 911 795
AgeI A'CCGG_T
Cuts at: 3166 3166
Size: 4778
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 933 933
Size: 4778
AlwNI CAG_nnn'CTG
Cuts at: 619 2083 3650 619
Size: 1464 1567 1747
Fragments arranged by size:
1747 1567 1464
ApaLI G'TGCA_C
Cuts at: 3548 3548
Size: 4778
ApoI r'AATT_y
Cuts at: 1096 1782 4131 4387 1096
Size: 686 2349 256 1487
Fragments arranged by size:
2349 1487 686 256
AseI AT'TA_AT
Cuts at: 20 740 20
Size: 720 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
AvaI C'yCGr_G
Cuts at: 1798 1798
Size: 4778
AvrII C'CTAG_G
Cuts at: 607 607
Size: 4778
BamHI G'GATC_C
Cuts at: 1803 1803
Size: 4778
BanI G'GyrC_C
Cuts at: 553 834 1794 2208 2456 2491 553
Size: 281 960 414 248 35 2840
Fragments arranged by size:
2840 960 414 281 248 35
BanII G_rGCy'C
Cuts at: 156 348 1792 2822 156
Size: 192 1444 1030 2112
Fragments arranged by size:
2112 1444 1030 192
BbeI G_GCGC'C
Cuts at: 2212 2460 2212
Size: 248 4530
BbvCI CC'TCA_GC
Cuts at: 569 620 722 569
Size: 51 102 4625
Fragments arranged by size:
4625 102 51
BciVI GTATCCnnnnn_n'
Cuts at: 2676 3437 4778 2676
Size: 761 1341 2676
Fragments arranged by size:
2676 1341 761
BclI T'GATC_A
Cuts at: 1568 1701 2298 1568
Size: 133 597 4048
Fragments arranged by size:
4048 597 133
BglII A'GATC_T
Cuts at: 2293 4330 2293
Size: 2037 2741
BlpI GC'TnA_GC
Cuts at: 1890 1890
Size: 4778
Bme1580I G_kGCm'C
Cuts at: 558 2403 2496 3552 558
Size: 1845 93 1056 1784
Fragments arranged by size:
1845 1784 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1990 2071 2406 4550 1990
Size: 81 335 2144 2218
Fragments arranged by size:
2218 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 4778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 791 218
Size: 573 4205
Bpu10I CC'TnA_GC
Cuts at: 569 620 722 569
Size: 51 102 4625
Fragments arranged by size:
4625 102 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1728 1954 2833 3325 3623 1728
Size: 226 879 492 298 2883
Fragments arranged by size:
2883 879 492 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 4482 4482
Size: 4778
BsaAI yAC'GTr
Cuts at: 1770 2131 1770
Size: 361 4417
BsaBI GATnn'nnATC
Cuts at: 6 825 2292 4329 6
Size: 819 1467 2037 455
Fragments arranged by size:
2037 1467 819 455
BsaHI Gr'CG_yC
Cuts at: 1014 2209 2457 3159 4655 1014
Size: 1195 248 702 1496 1137
Fragments arranged by size:
1496 1195 1137 702 248
BsaWI w'CCGG_w
Cuts at: 1967 2488 3166 3440 3587 3998 1967
Size: 521 678 274 147 411 2747
Fragments arranged by size:
2747 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 637 640 643 586
Size: 51 3 3 4721
Fragments arranged by size:
4721 51 3 3
BseYI C'CCAG_C
Cuts at: 130 400 463 589 2203 2961 3538 4034 130
Size: 270 63 126 1614 758 577 496 874
Fragments arranged by size:
1614 874 758 577 496 270 126 63
BsiEI CG_ry'CG
Cuts at: 104 2320 2366 3574 104
Size: 2216 46 1208 1308
Fragments arranged by size:
2216 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 348 1792 2570 2760 3552 348
Size: 1444 778 190 792 1574
Fragments arranged by size:
1574 1444 792 778 190
BsmI GAATG_Cn'
Cuts at: 1081 1081
Size: 4778
BsmBI CGTCTCn'nnnn_
Cuts at: 325 325
Size: 4778
Bsp1286I G_dGCh'C
Cuts at: 156 348 558 1792 2403 2496 2570 2760 2822
Size: 192 210 1234 611 93 74 190 62
Cuts at: 2822 3552 156
Size: 730 1382
Fragments arranged by size:
1382 1234 730 611 210 192 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3998 3998
Size: 4778
BspHI T'CATG_A
Cuts at: 3954 3954
Size: 4778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 591 1828 2042 2344 2725 591
Size: 1237 214 302 381 2644
Fragments arranged by size:
2644 1237 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3070 3124 4608 4773 50
Size: 3020 54 1484 165 55
Fragments arranged by size:
3020 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1172 2690 1172
Size: 1518 3260
BsrFI r'CCGG_y
Cuts at: 2776 2957 3166 2776
Size: 181 209 4388
Fragments arranged by size:
4388 209 181
BssSI C'ACGA_G
Cuts at: 3049 3407 3049
Size: 358 4420
BstAPI GCAn_nnn'nTGC
Cuts at: 1162 2155 1162
Size: 993 3785
BstBI TT'CG_AA
Cuts at: 110 3139 110
Size: 3029 1749
BstYI r'GATC_y
Cuts at: 1 826 1803 2270 2293 2628 2874 3886 3972
Size: 825 977 467 23 335 246 1012 86
Cuts at: 3972 3984 4330 1
Size: 12 346 449
Fragments arranged by size:
1012 977 825 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1488 1488
Size: 4778
BtgI C'CryG_G
Cuts at: 125 377 125
Size: 252 4526
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2728 2909 3072 2728
Size: 181 163 4434
Fragments arranged by size:
4434 181 163
BtsI GCAGTG_nn'
Cuts at: 311 2021 311
Size: 1710 3068
DraI TTT'AAA
Cuts at: 1532 1777 1532
Size: 245 4533
DrdI GACnn_nn'nnGTC
Cuts at: 2484 3342 2484
Size: 858 3920
EaeI y'GGCC_r
Cuts at: 1646 1727 2363 2537 2928 2955 4300 1646
Size: 81 636 174 391 27 1345 2124
Fragments arranged by size:
2124 1345 636 391 174 81 27
EagI C'GGCC_G
Cuts at: 2363 2363
Size: 4778
EarI CTCTTCn'nnn_
Cuts at: 464 500 629 2801 3011 464
Size: 36 129 2172 210 2231
Fragments arranged by size:
2231 2172 210 129 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2986 3296 3442 4588 2986
Size: 310 146 1146 3176
Fragments arranged by size:
3176 1146 310 146
EcoICRI GAG'CTC
Cuts at: 346 1790 346
Size: 1444 3334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 791 902 2603 3035 3782 4050 218
Size: 573 111 1701 432 747 268 946
Fragments arranged by size:
1701 946 747 573 432 268 111
EcoNI CCTnn'n_nnAGG
Cuts at: 611 611
Size: 4778
EcoO109I rG'GnC_Cy
Cuts at: 1917 1917
Size: 4778
EcoRI G'AATT_C
Cuts at: 1782 1782
Size: 4778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 872 1648 2797 872
Size: 776 1149 2853
Fragments arranged by size:
2853 1149 776
FspI TGC'GCA
Cuts at: 2559 2559
Size: 4778
HaeII r_GCGC'y
Cuts at: 2212 2460 3482 4461 2212
Size: 248 1022 979 2529
Fragments arranged by size:
2529 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 999 1004 1031 1036 1553 1585 2620 2650 2652
Size: 5 27 5 517 32 1035 30 2
Cuts at: 2652 2682 999
Size: 30 3095
Fragments arranged by size:
3095 1035 517 32 30 30 27 5
5 2
HincII GTy'rAC
Cuts at: 1127 1817 1127
Size: 690 4088
HindIII A'AGCT_T
Cuts at: 1239 1239
Size: 4778
KasI G'GCGC_C
Cuts at: 2208 2456 2208
Size: 248 4530
KpnI G_GTAC'C
Cuts at: 1798 1798
Size: 4778
MfeI C'AATT_G
Cuts at: 1688 1688
Size: 4778
MluI A'CGCG_T
Cuts at: 4211 4211
Size: 4778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 557 1573 3449 3633 557
Size: 1016 1876 184 1702
Fragments arranged by size:
1876 1702 1016 184
MscI TGG'CCA
Cuts at: 1648 1729 2539 1648
Size: 81 810 3887
Fragments arranged by size:
3887 810 81
MslI CAynn'nnrTG
Cuts at: 2767 2894 2767
Size: 127 4651
MspA1I CmG'CkG
Cuts at: 811 880 1889 2203 2563 3576 3821 811
Size: 69 1009 314 360 1013 245 1768
Fragments arranged by size:
1768 1013 1009 360 314 245 69
NaeI GCC'GGC
Cuts at: 2959 2959
Size: 4778
NarI GG'CG_CC
Cuts at: 2209 2457 2209
Size: 248 4530
NcoI C'CATG_G
Cuts at: 125 377 125
Size: 252 4526
NdeI CA'TA_TG
Cuts at: 1346 1346
Size: 4778
NgoMIV G'CCGG_C
Cuts at: 2957 2957
Size: 4778
NspI r_CATG'y
Cuts at: 428 1339 1831 2331 3238 428
Size: 911 492 500 907 1968
Fragments arranged by size:
1968 911 907 500 492
PciI A'CATG_T
Cuts at: 424 1335 3234 424
Size: 911 1899 1968
Fragments arranged by size:
1968 1899 911
PflMI CCAn_nnn'nTGG
Cuts at: 764 764
Size: 4778
PmeI GTTT'AAAC
Cuts at: 1777 1777
Size: 4778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 925 957 3917 3949 925
Size: 32 2960 32 1754
Fragments arranged by size:
2960 1754 32 32
PsiI TTA'TAA
Cuts at: 1715 1715
Size: 4778
PstI C_TGCA'G
Cuts at: 1557 1825 2510 1557
Size: 268 685 3825
Fragments arranged by size:
3825 685 268
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4778
PvuII CAG'CTG
Cuts at: 811 2203 2563 811
Size: 1392 360 3026
Fragments arranged by size:
3026 1392 360
RsrII CG'GwC_CG
Cuts at: 2973 2973
Size: 4778
SacI G_AGCT'C
Cuts at: 348 1792 348
Size: 1444 3334
SalI G'TCGA_C
Cuts at: 1815 1815
Size: 4778
SapI GCTCTTCn'nnn_
Cuts at: 2801 3011 2801
Size: 210 4568
SbfI CC_TGCA'GG
Cuts at: 1825 1825
Size: 4778
ScaI AGT'ACT
Cuts at: 4295 4295
Size: 4778
SfcI C'TryA_G
Cuts at: 33 233 1553 1821 2506 3499 3690 33
Size: 200 1320 268 685 993 191 1121
Fragments arranged by size:
1320 1121 993 685 268 200 191
SfoI GGC'GCC
Cuts at: 2210 2458 2210
Size: 248 4530
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
SmaI CCC'GGG
Cuts at: 1800 1800
Size: 4778
SmlI C'TyrA_G
Cuts at: 1120 1707 1933 2848 3340 3602 1120
Size: 587 226 915 492 262 2296
Fragments arranged by size:
2296 915 587 492 262 226
SnaBI TAC'GTA
Cuts at: 1770 1770
Size: 4778
SpeI A'CTAG_T
Cuts at: 67 676 67
Size: 609 4169
SphI G_CATG'C
Cuts at: 1831 2331 1831
Size: 500 4278
StuI AGG'CCT
Cuts at: 231 231
Size: 4778
StyI C'CwwG_G
Cuts at: 125 377 395 607 758 1912 2266 125
Size: 252 18 212 151 1154 354 2637
Fragments arranged by size:
2637 1154 354 252 212 151 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1159 1202 2361 2494 2953 3162 1159
Size: 43 1159 133 459 209 2775
Fragments arranged by size:
2775 1159 459 209 133 43
TatI w'GTAC_w
Cuts at: 4293 4293
Size: 4778
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 941 973 4295 4327 941
Size: 32 3322 32 1392
Fragments arranged by size:
3322 1392 32 32
Tth111I GACn'n_nGTC
Cuts at: 270 2575 270
Size: 2305 2473
XbaI T'CTAG_A
Cuts at: 1809 1809
Size: 4778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2273 2273
Size: 4778
XmaI C'CCGG_G
Cuts at: 1798 1798
Size: 4778
ZraI GAC'GTC
Cuts at: 1015 1015
Size: 4778
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AloI ApaI AscI BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI SwaI XhoI XmnI