Restriction map for
pF1KSDA1704
(Circular) (Six-base) MAPSORT of: pF1KSDA1704.seq Check: 5859 from: 1 to: 4157
pF1KSDA1704 4157 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1195 1195
Size: 4157
Acc65I G'GTAC_C
Cuts at: 1173 1173
Size: 4157
AclI AA'CG_TT
Cuts at: 3997 3997
Size: 4157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 1013 1982 2414 3161 3429 338
Size: 675 969 432 747 268 1066
Fragments arranged by size:
1066 969 747 675 432 268
AflIII A'CryG_T
Cuts at: 1509 2613 3590 1509
Size: 1104 977 2076
Fragments arranged by size:
2076 1104 977
AgeI A'CCGG_T
Cuts at: 2545 2545
Size: 4157
AlwNI CAG_nnn'CTG
Cuts at: 1462 3029 1462
Size: 1567 2590
ApaI G_GGCC'C
Cuts at: 630 630
Size: 4157
ApaLI G'TGCA_C
Cuts at: 2927 2927
Size: 4157
ApoI r'AATT_y
Cuts at: 1161 3510 3766 1161
Size: 2349 256 1552
Fragments arranged by size:
2349 1552 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4157
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
AvaI C'yCGr_G
Cuts at: 204 1177 204
Size: 973 3184
BamHI G'GATC_C
Cuts at: 1182 1182
Size: 4157
BanI G'GyrC_C
Cuts at: 1173 1587 1835 1870 1173
Size: 414 248 35 3460
Fragments arranged by size:
3460 414 248 35
BanII G_rGCy'C
Cuts at: 211 630 1171 2201 211
Size: 419 541 1030 2167
Fragments arranged by size:
2167 1030 541 419
BbeI G_GCGC'C
Cuts at: 1591 1839 1591
Size: 248 3909
BbsI GAAGACnn'nnnn_
Cuts at: 284 1001 284
Size: 717 3440
BciVI GTATCCnnnnn_n'
Cuts at: 2055 2816 4157 2055
Size: 761 1341 2055
Fragments arranged by size:
2055 1341 761
BclI T'GATC_A
Cuts at: 1677 1677
Size: 4157
BglII A'GATC_T
Cuts at: 1041 1672 3709 1041
Size: 631 2037 1489
Fragments arranged by size:
2037 1489 631
BlpI GC'TnA_GC
Cuts at: 908 1269 908
Size: 361 3796
Bme1580I G_kGCm'C
Cuts at: 630 1782 1875 2931 630
Size: 1152 93 1056 1856
Fragments arranged by size:
1856 1152 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1369 1450 1785 3929 1369
Size: 81 335 2144 1597
Fragments arranged by size:
2144 1597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 788 797 698
Size: 90 9 4058
Fragments arranged by size:
4058 90 9
Bpu10I CC'TnA_GC
Cuts at: 827 827
Size: 4157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1333 2212 2704 3002 1030
Size: 303 879 492 298 2185
Fragments arranged by size:
2185 879 492 303 298
BsaI GGTCTCn'nnnn_
Cuts at: 128 3861 128
Size: 3733 424
BsaAI yAC'GTr
Cuts at: 1149 1510 1149
Size: 361 3796
BsaBI GATnn'nnATC
Cuts at: 6 945 1040 1671 3708 6
Size: 939 95 631 2037 455
Fragments arranged by size:
2037 939 631 455 95
BsaHI Gr'CG_yC
Cuts at: 1588 1836 2538 4034 1588
Size: 248 702 1496 1711
Fragments arranged by size:
1711 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1346 1867 2545 2819 2966 3377 1346
Size: 521 678 274 147 411 2126
Fragments arranged by size:
2126 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 786 816 786
Size: 30 4127
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 431 431
Size: 4157
BseYI C'CCAG_C
Cuts at: 1582 2340 2917 3413 1582
Size: 758 577 496 2326
Fragments arranged by size:
2326 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1699 1745 2953 104
Size: 1595 46 1208 1308
Fragments arranged by size:
1595 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1171 1949 2139 2931 1171
Size: 778 190 792 2397
Fragments arranged by size:
2397 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 211 630 1171 1782 1875 1949 2139 2201 2931
Size: 419 541 611 93 74 190 62 730
Cuts at: 2931 211
Size: 1437
Fragments arranged by size:
1437 730 611 541 419 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3377 3377
Size: 4157
BspHI T'CATG_A
Cuts at: 3333 3333
Size: 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 903 1207 1421 1723 2104 903
Size: 304 214 302 381 2956
Fragments arranged by size:
2956 381 304 302 214
BsrBI CCG'CTC
Cuts at: 50 198 2449 2503 3987 4152 50
Size: 148 2251 54 1484 165 55
Fragments arranged by size:
2251 1484 165 148 55 54
BsrDI GCAATG_nn'
Cuts at: 466 2069 466
Size: 1603 2554
BsrFI r'CCGG_y
Cuts at: 147 2155 2336 2545 147
Size: 2008 181 209 1759
Fragments arranged by size:
2008 1759 209 181
BssSI C'ACGA_G
Cuts at: 2428 2786 2428
Size: 358 3799
BstAPI GCAn_nnn'nTGC
Cuts at: 1534 1534
Size: 4157
BstBI TT'CG_AA
Cuts at: 110 2518 110
Size: 2408 1749
BstYI r'GATC_y
Cuts at: 1 1041 1182 1649 1672 2007 2253 3265 3351
Size: 1040 141 467 23 335 246 1012 86
Cuts at: 3351 3363 3709 1
Size: 12 346 449
Fragments arranged by size:
1040 1012 467 449 346 335 246 141
86 23 12
BtgI C'CryG_G
Cuts at: 125 174 125
Size: 49 4108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 287 2107 2288 2451 287
Size: 1820 181 163 1993
Fragments arranged by size:
1993 1820 181 163
BtsI GCAGTG_nn'
Cuts at: 552 1400 552
Size: 848 3309
ClaI AT'CG_AT
Cuts at: 783 783
Size: 4157
DraI TTT'AAA
Cuts at: 423 1156 423
Size: 733 3424
DrdI GACnn_nn'nnGTC
Cuts at: 1863 2721 1863
Size: 858 3299
EaeI y'GGCC_r
Cuts at: 1742 1916 2307 2334 3679 1742
Size: 174 391 27 1345 2220
Fragments arranged by size:
2220 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1742 1742
Size: 4157
EarI CTCTTCn'nnn_
Cuts at: 188 753 885 2180 2390 188
Size: 565 132 1295 210 1955
Fragments arranged by size:
1955 1295 565 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2365 2675 2821 3967 2365
Size: 310 146 1146 2555
Fragments arranged by size:
2555 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1169 1169
Size: 4157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 698 788 797 1013 1982 2414 3161 3429
Size: 360 90 9 216 969 432 747 268
Cuts at: 3429 338
Size: 1066
Fragments arranged by size:
1066 969 747 432 360 268 216 90
9
EcoNI CCTnn'n_nnAGG
Cuts at: 215 581 215
Size: 366 3791
EcoO109I rG'GnC_Cy
Cuts at: 201 460 626 1296 201
Size: 259 166 670 3062
Fragments arranged by size:
3062 670 259 166
EcoRI G'AATT_C
Cuts at: 1161 1161
Size: 4157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 4157
FspI TGC'GCA
Cuts at: 1938 1938
Size: 4157
HaeII r_GCGC'y
Cuts at: 1591 1839 2861 3840 1591
Size: 248 1022 979 1908
Fragments arranged by size:
1908 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 345 377 655 687 757 786 789
Size: 32 188 32 278 32 70 29 3
Cuts at: 789 818 901 933 1999 2029 2031 2061 125
Size: 29 83 32 1066 30 2 30 2221
Fragments arranged by size:
2221 1066 278 188 83 70 32 32
32 32 30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 594 1196 594
Size: 602 3555
HindIII A'AGCT_T
Cuts at: 1006 1006
Size: 4157
HpaI GTT'AAC
Cuts at: 594 594
Size: 4157
KasI G'GCGC_C
Cuts at: 1587 1835 1587
Size: 248 3909
KpnI G_GTAC'C
Cuts at: 1177 1177
Size: 4157
MluI A'CGCG_T
Cuts at: 3590 3590
Size: 4157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 361 2828 3012 361
Size: 2467 184 1506
Fragments arranged by size:
2467 1506 184
MscI TGG'CCA
Cuts at: 1918 1918
Size: 4157
MslI CAynn'nnrTG
Cuts at: 2146 2273 2146
Size: 127 4030
MspA1I CmG'CkG
Cuts at: 176 184 808 1268 1582 1942 2955 3200 176
Size: 8 624 460 314 360 1013 245 1133
Fragments arranged by size:
1133 1013 624 460 360 314 245 8
NaeI GCC'GGC
Cuts at: 2338 2338
Size: 4157
NarI GG'CG_CC
Cuts at: 1588 1836 1588
Size: 248 3909
NcoI C'CATG_G
Cuts at: 125 125
Size: 4157
NgoMIV G'CCGG_C
Cuts at: 2336 2336
Size: 4157
NspI r_CATG'y
Cuts at: 1210 1710 2617 1210
Size: 500 907 2750
Fragments arranged by size:
2750 907 500
PciI A'CATG_T
Cuts at: 2613 2613
Size: 4157
PfoI T'CCnGG_A
Cuts at: 414 522 414
Size: 108 4049
PmeI GTTT'AAAC
Cuts at: 1156 1156
Size: 4157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 4125
PpuMI rG'GwC_Cy
Cuts at: 201 460 201
Size: 259 3898
PshAI GACnn'nnGTC
Cuts at: 332 731 332
Size: 399 3758
PsiI TTA'TAA
Cuts at: 242 242
Size: 4157
PspOMI G'GGCC_C
Cuts at: 626 626
Size: 4157
PstI C_TGCA'G
Cuts at: 1204 1889 1204
Size: 685 3472
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4157
PvuII CAG'CTG
Cuts at: 808 1582 1942 808
Size: 774 360 3023
Fragments arranged by size:
3023 774 360
RsrII CG'GwC_CG
Cuts at: 145 2352 145
Size: 2207 1950
SacI G_AGCT'C
Cuts at: 1171 1171
Size: 4157
SacII CC_GC'GG
Cuts at: 177 177
Size: 4157
SalI G'TCGA_C
Cuts at: 1194 1194
Size: 4157
SanDI GG'GwC_CC
Cuts at: 201 201
Size: 4157
SapI GCTCTTCn'nnn_
Cuts at: 188 753 2180 2390 188
Size: 565 1427 210 1955
Fragments arranged by size:
1955 1427 565 210
SbfI CC_TGCA'GG
Cuts at: 1204 1204
Size: 4157
ScaI AGT'ACT
Cuts at: 3674 3674
Size: 4157
SexAI A'CCwGG_T
Cuts at: 474 474
Size: 4157
SfcI C'TryA_G
Cuts at: 33 1200 1885 2878 3069 33
Size: 1167 685 993 191 1121
Fragments arranged by size:
1167 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1589 1837 1589
Size: 248 3909
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
SmaI CCC'GGG
Cuts at: 1179 1179
Size: 4157
SmlI C'TyrA_G
Cuts at: 1045 1312 2227 2719 2981 1045
Size: 267 915 492 262 2221
Fragments arranged by size:
2221 915 492 267 262
SnaBI TAC'GTA
Cuts at: 1149 1149
Size: 4157
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4157
SphI G_CATG'C
Cuts at: 1210 1710 1210
Size: 500 3657
StyI C'CwwG_G
Cuts at: 125 445 742 1291 1645 125
Size: 320 297 549 354 2637
Fragments arranged by size:
2637 549 354 320 297
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1740 1873 2332 2541 1740
Size: 133 459 209 3356
Fragments arranged by size:
3356 459 209 133
TatI w'GTAC_w
Cuts at: 3672 3672
Size: 4157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4125
Tth111I GACn'n_nGTC
Cuts at: 1954 1954
Size: 4157
XbaI T'CTAG_A
Cuts at: 1096 1188 1096
Size: 92 4065
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1652 1652
Size: 4157
XmaI C'CCGG_G
Cuts at: 1177 1177
Size: 4157
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PspXI PsrI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI ZraI