Restriction map for
pF1KSDA1706
(Circular) (Six-base) MAPSORT of: pF1KSDA1706.seq Check: 4445 from: 1 to: 4844
pF1KSDA1706 4844 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1882 1882
Size: 4844
Acc65I G'GTAC_C
Cuts at: 1860 1860
Size: 4844
AclI AA'CG_TT
Cuts at: 4684 4684
Size: 4844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 2669 3101 3848 4116 198
Size: 2471 432 747 268 926
Fragments arranged by size:
2471 926 747 432 268
AflIII A'CryG_T
Cuts at: 295 531 2196 3300 4277 295
Size: 236 1665 1104 977 862
Fragments arranged by size:
1665 1104 977 862 236
AgeI A'CCGG_T
Cuts at: 1397 3232 1397
Size: 1835 3009
AleI CACnn'nnGTG
Cuts at: 514 514
Size: 4844
AlwNI CAG_nnn'CTG
Cuts at: 2149 3716 2149
Size: 1567 3277
ApaI G_GGCC'C
Cuts at: 635 839 1112 1276 635
Size: 204 273 164 4203
Fragments arranged by size:
4203 273 204 164
ApaLI G'TGCA_C
Cuts at: 3614 3614
Size: 4844
ApoI r'AATT_y
Cuts at: 1848 4197 4453 1848
Size: 2349 256 2239
Fragments arranged by size:
2349 2239 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4844
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
AvaI C'yCGr_G
Cuts at: 780 961 1112 1288 1864 780
Size: 181 151 176 576 3760
Fragments arranged by size:
3760 576 181 176 151
BamHI G'GATC_C
Cuts at: 695 1869 695
Size: 1174 3670
BanI G'GyrC_C
Cuts at: 385 701 1860 2274 2522 2557 385
Size: 316 1159 414 248 35 2672
Fragments arranged by size:
2672 1159 414 316 248 35
BanII G_rGCy'C
Cuts at: 635 787 839 1112 1243 1276 1858 2888 635
Size: 152 52 273 131 33 582 1030 2591
Fragments arranged by size:
2591 1030 582 273 152 131 52 33
BbeI G_GCGC'C
Cuts at: 389 2278 2526 389
Size: 1889 248 2707
Fragments arranged by size:
2707 1889 248
BbvCI CC'TCA_GC
Cuts at: 601 907 601
Size: 306 4538
BciVI GTATCCnnnnn_n'
Cuts at: 2742 3503 4844 2742
Size: 761 1341 2742
Fragments arranged by size:
2742 1341 761
BclI T'GATC_A
Cuts at: 2364 2364
Size: 4844
BglI GCCn_nnn'nGGC
Cuts at: 556 834 556
Size: 278 4566
BglII A'GATC_T
Cuts at: 416 2359 4396 416
Size: 1943 2037 864
Fragments arranged by size:
2037 1943 864
BlpI GC'TnA_GC
Cuts at: 441 1956 441
Size: 1515 3329
Bme1580I G_kGCm'C
Cuts at: 521 635 839 1112 1259 1276 2469 2562 3618
Size: 114 204 273 147 17 1193 93 1056
Cuts at: 3618 521
Size: 1747
Fragments arranged by size:
1747 1193 1056 273 204 147 114 93
17
BmrI ACTGGGnnnn_n'
Cuts at: 452 1650 1717 2056 2137 2472 4616 452
Size: 1198 67 339 81 335 2144 680
Fragments arranged by size:
2144 1198 680 339 335 81 67
BmtI G_CTAG'C
Cuts at: 484 1732 484
Size: 1248 3596
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1164 1678 3211 1164
Size: 514 1533 2797
Fragments arranged by size:
2797 1533 514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1144 1776 418
Size: 726 632 3486
Fragments arranged by size:
3486 726 632
Bpu10I CC'TnA_GC
Cuts at: 601 796 907 601
Size: 195 111 4538
Fragments arranged by size:
4538 195 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1559 2020 2899 3391 3689 1559
Size: 461 879 492 298 2714
Fragments arranged by size:
2714 879 492 461 298
BsaI GGTCTCn'nnnn_
Cuts at: 4548 4548
Size: 4844
BsaAI yAC'GTr
Cuts at: 532 1836 2197 532
Size: 1304 361 3179
Fragments arranged by size:
3179 1304 361
BsaBI GATnn'nnATC
Cuts at: 6 2358 4395 6
Size: 2352 2037 455
Fragments arranged by size:
2352 2037 455
BsaHI Gr'CG_yC
Cuts at: 386 1769 2275 2523 3225 4721 386
Size: 1383 506 248 702 1496 509
Fragments arranged by size:
1496 1383 702 509 506 248
BsaWI w'CCGG_w
Cuts at: 1397 1680 2033 2554 3232 3506 3653 4064 1397
Size: 283 353 521 678 274 147 411 2177
Fragments arranged by size:
2177 678 521 411 353 283 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 283 280
Size: 3 4841
BseYI C'CCAG_C
Cuts at: 560 1171 1276 1372 2269 3027 3604 4100 560
Size: 611 105 96 897 758 577 496 1304
Fragments arranged by size:
1304 897 758 611 577 496 105 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1044 1044
Size: 4844
BsiEI CG_ry'CG
Cuts at: 104 862 2386 2432 3640 104
Size: 758 1524 46 1208 1308
Fragments arranged by size:
1524 1308 1208 758 46
BsiHKAI G_wGCw'C
Cuts at: 136 1243 1858 2636 2826 3618 136
Size: 1107 615 778 190 792 1362
Fragments arranged by size:
1362 1107 792 778 615 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1214 1214
Size: 4844
Bsp1286I G_dGCh'C
Cuts at: 136 521 635 787 839 1112 1243 1259 1276
Size: 385 114 152 52 273 131 16 17
Cuts at: 1276 1858 2469 2562 2636 2826 2888 3618 136
Size: 582 611 93 74 190 62 730 1362
Fragments arranged by size:
1362 730 611 582 385 273 190 152
131 114 93 74 62 52 17 16
BspEI T'CCGG_A
Cuts at: 1680 4064 1680
Size: 2384 2460
BspHI T'CATG_A
Cuts at: 566 4020 566
Size: 3454 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 393 485 830 1894 2108 2410 2791 393
Size: 92 345 1064 214 302 381 2446
Fragments arranged by size:
2446 1064 381 345 302 214 92
BsrBI CCG'CTC
Cuts at: 50 152 240 3136 3190 4674 4839 50
Size: 102 88 2896 54 1484 165 55
Fragments arranged by size:
2896 1484 165 102 88 55 54
BsrDI GCAATG_nn'
Cuts at: 1497 2756 1497
Size: 1259 3585
BsrFI r'CCGG_y
Cuts at: 1204 1397 2842 3023 3232 1204
Size: 193 1445 181 209 2816
Fragments arranged by size:
2816 1445 209 193 181
BssSI C'ACGA_G
Cuts at: 1825 3115 3473 1825
Size: 1290 358 3196
Fragments arranged by size:
3196 1290 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1029 2221 1029
Size: 1192 3652
BstBI TT'CG_AA
Cuts at: 110 3205 110
Size: 3095 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 396 396
Size: 4844
BstYI r'GATC_y
Cuts at: 1 416 695 1869 2336 2359 2694 2940 3952
Size: 415 279 1174 467 23 335 246 1012
Cuts at: 3952 4038 4050 4396 1
Size: 86 12 346 449
Fragments arranged by size:
1174 1012 467 449 415 346 335 279
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1507 1507
Size: 4844
BtgI C'CryG_G
Cuts at: 125 260 302 866 941 125
Size: 135 42 564 75 4028
Fragments arranged by size:
4028 564 135 75 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2794 2975 3138 2794
Size: 181 163 4500
Fragments arranged by size:
4500 181 163
BtsI GCAGTG_nn'
Cuts at: 254 1716 2087 254
Size: 1462 371 3011
Fragments arranged by size:
3011 1462 371
DraI TTT'AAA
Cuts at: 1843 1843
Size: 4844
DraIII CAC_nnn'GTG
Cuts at: 708 708
Size: 4844
DrdI GACnn_nn'nnGTC
Cuts at: 2550 3408 2550
Size: 858 3986
EaeI y'GGCC_r
Cuts at: 506 859 914 1392 1473 2429 2603 2994 3021
Size: 353 55 478 81 956 174 391 27
Cuts at: 3021 4366 506
Size: 1345 984
Fragments arranged by size:
1345 984 956 478 391 353 174 81
55 27
EagI C'GGCC_G
Cuts at: 859 2429 859
Size: 1570 3274
EarI CTCTTCn'nnn_
Cuts at: 2867 3077 2867
Size: 210 4634
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 607 3052 3362 3508 4654 607
Size: 2445 310 146 1146 797
Fragments arranged by size:
2445 1146 797 310 146
EcoICRI GAG'CTC
Cuts at: 1241 1856 1241
Size: 615 4229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 418 1144 1776 2669 3101 3848 4116 198
Size: 220 726 632 893 432 747 268 926
Fragments arranged by size:
926 893 747 726 632 432 268 220
EcoO109I rG'GnC_Cy
Cuts at: 165 360 474 632 651 728 813 835 1108
Size: 195 114 158 19 77 85 22 273
Cuts at: 1108 1109 1273 1983 165
Size: 1 164 710 3026
Fragments arranged by size:
3026 710 273 195 164 158 114 85
77 22 19 1
EcoRI G'AATT_C
Cuts at: 1848 1848
Size: 4844
EcoRV GAT'ATC
Cuts at: 1503 1503
Size: 4844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1048 2863 1048
Size: 1815 3029
FspI TGC'GCA
Cuts at: 2625 2625
Size: 4844
HaeII r_GCGC'y
Cuts at: 389 2278 2526 3548 4527 389
Size: 1889 248 1022 979 706
Fragments arranged by size:
1889 1022 979 706 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 320 352 1353 1385 1675 1707 2686 2716 2718
Size: 32 1001 32 290 32 979 30 2
Cuts at: 2718 2748 320
Size: 30 2416
Fragments arranged by size:
2416 1001 979 290 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 537 1329 1883 537
Size: 792 554 3498
Fragments arranged by size:
3498 792 554
HindIII A'AGCT_T
Cuts at: 1009 1614 1009
Size: 605 4239
HpaI GTT'AAC
Cuts at: 537 1329 537
Size: 792 4052
KasI G'GCGC_C
Cuts at: 385 2274 2522 385
Size: 1889 248 2707
Fragments arranged by size:
2707 1889 248
KpnI G_GTAC'C
Cuts at: 1864 1864
Size: 4844
MluI A'CGCG_T
Cuts at: 295 4277 295
Size: 3982 862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3515 3699 3515
Size: 184 4660
MscI TGG'CCA
Cuts at: 508 916 1394 1475 2605 508
Size: 408 478 81 1130 2747
Fragments arranged by size:
2747 1130 478 408 81
MslI CAynn'nnrTG
Cuts at: 226 514 571 1388 2833 2960 226
Size: 288 57 817 1445 127 2110
Fragments arranged by size:
2110 1445 817 288 127 57
MspA1I CmG'CkG
Cuts at: 514 1787 1955 2269 2629 3642 3887 514
Size: 1273 168 314 360 1013 245 1471
Fragments arranged by size:
1471 1273 1013 360 314 245 168
NaeI GCC'GGC
Cuts at: 1206 3025 1206
Size: 1819 3025
NarI GG'CG_CC
Cuts at: 386 2275 2523 386
Size: 1889 248 2707
Fragments arranged by size:
2707 1889 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4844
NgoMIV G'CCGG_C
Cuts at: 1204 3023 1204
Size: 1819 3025
NheI G'CTAG_C
Cuts at: 480 1728 480
Size: 1248 3596
NspI r_CATG'y
Cuts at: 1897 2397 3304 1897
Size: 500 907 3437
Fragments arranged by size:
3437 907 500
PasI CC'CwG_GG
Cuts at: 138 161 138
Size: 23 4821
PciI A'CATG_T
Cuts at: 3300 3300
Size: 4844
PflMI CCAn_nnn'nTGG
Cuts at: 1401 1401
Size: 4844
PfoI T'CCnGG_A
Cuts at: 686 992 686
Size: 306 4538
PmeI GTTT'AAAC
Cuts at: 1843 1843
Size: 4844
PmlI CAC'GTG
Cuts at: 532 532
Size: 4844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 4812
PpuMI rG'GwC_Cy
Cuts at: 165 360 474 651 813 165
Size: 195 114 177 162 4196
Fragments arranged by size:
4196 195 177 162 114
PspOMI G'GGCC_C
Cuts at: 631 835 1108 1272 631
Size: 204 273 164 4203
Fragments arranged by size:
4203 273 204 164
PstI C_TGCA'G
Cuts at: 806 1891 2576 806
Size: 1085 685 3074
Fragments arranged by size:
3074 1085 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4844
PvuII CAG'CTG
Cuts at: 514 2269 2629 514
Size: 1755 360 2729
Fragments arranged by size:
2729 1755 360
RsrII CG'GwC_CG
Cuts at: 3039 3039
Size: 4844
SacI G_AGCT'C
Cuts at: 1243 1858 1243
Size: 615 4229
SalI G'TCGA_C
Cuts at: 1881 1881
Size: 4844
SanDI GG'GwC_CC
Cuts at: 165 165
Size: 4844
SapI GCTCTTCn'nnn_
Cuts at: 2867 3077 2867
Size: 210 4634
SbfI CC_TGCA'GG
Cuts at: 1891 1891
Size: 4844
ScaI AGT'ACT
Cuts at: 4361 4361
Size: 4844
SexAI A'CCwGG_T
Cuts at: 704 704
Size: 4844
SfcI C'TryA_G
Cuts at: 33 802 1423 1887 2572 3565 3756 33
Size: 769 621 464 685 993 191 1121
Fragments arranged by size:
1121 993 769 685 621 464 191
SfoI GGC'GCC
Cuts at: 387 2276 2524 387
Size: 1889 248 2707
Fragments arranged by size:
2707 1889 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
SmaI CCC'GGG
Cuts at: 963 1114 1866 963
Size: 151 752 3941
Fragments arranged by size:
3941 752 151
SmlI C'TyrA_G
Cuts at: 1574 1999 2914 3406 3668 1574
Size: 425 915 492 262 2750
Fragments arranged by size:
2750 915 492 425 262
SnaBI TAC'GTA
Cuts at: 1836 1836
Size: 4844
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4844
SphI G_CATG'C
Cuts at: 1897 2397 1897
Size: 500 4344
StuI AGG'CCT
Cuts at: 581 874 900 1045 1661 581
Size: 293 26 145 616 3764
Fragments arranged by size:
3764 616 293 145 26
StyI C'CwwG_G
Cuts at: 125 1046 1476 1672 1796 1978 2332 125
Size: 921 430 196 124 182 354 2637
Fragments arranged by size:
2637 921 430 354 196 182 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 754 856 2427 2560 3019 3228 754
Size: 102 1571 133 459 209 2370
Fragments arranged by size:
2370 1571 459 209 133 102
TatI w'GTAC_w
Cuts at: 4359 4359
Size: 4844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4361 4393 4361
Size: 32 4812
Tth111I GACn'n_nGTC
Cuts at: 2641 2641
Size: 4844
XbaI T'CTAG_A
Cuts at: 1875 1875
Size: 4844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1168 2339 1168
Size: 1171 3673
XmaI C'CCGG_G
Cuts at: 961 1112 1864 961
Size: 151 752 3941
Fragments arranged by size:
3941 752 151
XmnI GAAnn'nnTTC
Cuts at: 1627 1627
Size: 4844
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI
FseI FspAI MfeI NdeI NotI NruI NsiI PacI
PshAI PsiI PspXI PsrI SacII SfiI SgrAI SrfI
SspI SwaI XhoI ZraI