Restriction map for
pF1KSDA1709
(Circular) (Six-base) MAPSORT of: pF1KSDA1709.seq Check: 9189 from: 1 to: 6212
pF1KSDA1709 6212 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1788 1788
Size: 6212
AccI GT'mk_AC
Cuts at: 3250 3250
Size: 6212
Acc65I G'GTAC_C
Cuts at: 3228 3228
Size: 6212
AclI AA'CG_TT
Cuts at: 6052 6052
Size: 6212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 1652 1712 1910 2392 2611 3010 3208 4037
Size: 1383 60 198 482 219 399 198 829
Cuts at: 4037 4469 5216 5484 269
Size: 432 747 268 997
Fragments arranged by size:
1383 997 829 747 482 432 399 268
219 198 198 60
AflIII A'CryG_T
Cuts at: 251 3564 4668 5645 251
Size: 3313 1104 977 818
Fragments arranged by size:
3313 1104 977 818
AgeI A'CCGG_T
Cuts at: 4600 4600
Size: 6212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 6212
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1988 2020 1988
Size: 32 6180
AlwNI CAG_nnn'CTG
Cuts at: 1252 2067 3517 5084 1252
Size: 815 1450 1567 2380
Fragments arranged by size:
2380 1567 1450 815
ApaLI G'TGCA_C
Cuts at: 4982 4982
Size: 6212
ApoI r'AATT_y
Cuts at: 922 1096 1174 2132 2953 3216 5565 5821 922
Size: 174 78 958 821 263 2349 256 1313
Fragments arranged by size:
2349 1313 958 821 263 256 174 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 6212
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6212
AvaI C'yCGr_G
Cuts at: 980 1910 2787 3232 980
Size: 930 877 445 3960
Fragments arranged by size:
3960 930 877 445
BamHI G'GATC_C
Cuts at: 3237 3237
Size: 6212
BanI G'GyrC_C
Cuts at: 3228 3642 3890 3925 3228
Size: 414 248 35 5515
Fragments arranged by size:
5515 414 248 35
BanII G_rGCy'C
Cuts at: 2909 3226 4256 2909
Size: 317 1030 4865
Fragments arranged by size:
4865 1030 317
BbeI G_GCGC'C
Cuts at: 3646 3894 3646
Size: 248 5964
BbsI GAAGACnn'nnnn_
Cuts at: 2380 2923 2380
Size: 543 5669
BbvCI CC'TCA_GC
Cuts at: 829 829
Size: 6212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 459 493 1762 1796 459
Size: 34 1269 34 4875
Fragments arranged by size:
4875 1269 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4110 4871 6212 4110
Size: 761 1341 4110
Fragments arranged by size:
4110 1341 761
BclI T'GATC_A
Cuts at: 1190 3732 1190
Size: 2542 3670
BfrBI ATG'CAT
Cuts at: 129 129
Size: 6212
BglI GCCn_nnn'nGGC
Cuts at: 1331 1486 1331
Size: 155 6057
BglII A'GATC_T
Cuts at: 193 3727 5764 193
Size: 3534 2037 641
Fragments arranged by size:
3534 2037 641
BlpI GC'TnA_GC
Cuts at: 182 3324 182
Size: 3142 3070
Bme1580I G_kGCm'C
Cuts at: 1381 1817 3837 3930 4986 1381
Size: 436 2020 93 1056 2607
Fragments arranged by size:
2607 2020 1056 436 93
BmrI ACTGGGnnnn_n'
Cuts at: 3424 3505 3840 5984 3424
Size: 81 335 2144 3652
Fragments arranged by size:
3652 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 954 1684 2802 4579 954
Size: 730 1118 1777 2587
Fragments arranged by size:
2587 1777 1118 730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1504 1744 2122 2620 844
Size: 660 240 378 498 4436
Fragments arranged by size:
4436 660 498 378 240
Bpu10I CC'TnA_GC
Cuts at: 829 2548 829
Size: 1719 4493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 1384 2137 2269 3388 4267 4759 5057 580
Size: 804 753 132 1119 879 492 298 1735
Fragments arranged by size:
1735 1119 879 804 753 492 298 132
BsaI GGTCTCn'nnnn_
Cuts at: 445 5916 445
Size: 5471 741
BsaAI yAC'GTr
Cuts at: 1725 3204 3565 1725
Size: 1479 361 4372
Fragments arranged by size:
4372 1479 361
BsaBI GATnn'nnATC
Cuts at: 6 1488 3726 5763 6
Size: 1482 2238 2037 455
Fragments arranged by size:
2238 2037 1482 455
BsaHI Gr'CG_yC
Cuts at: 3643 3891 4593 6089 3643
Size: 248 702 1496 3766
Fragments arranged by size:
3766 1496 702 248
BsaWI w'CCGG_w
Cuts at: 155 329 795 2347 2813 3401 3922 4600 4874
Size: 174 466 1552 466 588 521 678 274
Cuts at: 4874 5021 5432 155
Size: 147 411 935
Fragments arranged by size:
1552 935 678 588 521 466 466 411
274 174 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2469 2499 2561 2591 2469
Size: 30 62 30 6090
Fragments arranged by size:
6090 62 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 744 969 2200 2272 2572 2884 744
Size: 225 1231 72 300 312 4072
Fragments arranged by size:
4072 1231 312 300 225 72
BseYI C'CCAG_C
Cuts at: 312 860 1021 1260 2537 2685 3061 3637 4395
Size: 548 161 239 1277 148 376 576 758
Cuts at: 4395 4972 5468 312
Size: 577 496 1056
Fragments arranged by size:
1277 1056 758 577 576 548 496 376
239 161 148
BsiEI CG_ry'CG
Cuts at: 104 3754 3800 5008 104
Size: 3650 46 1208 1308
Fragments arranged by size:
3650 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2058 2357 3226 4004 4194 4986 2058
Size: 299 869 778 190 792 3284
Fragments arranged by size:
3284 869 792 778 299 190
BsmI GAATG_Cn'
Cuts at: 347 627 1833 347
Size: 280 1206 4726
Fragments arranged by size:
4726 1206 280
Bsp1286I G_dGCh'C
Cuts at: 1381 1817 2058 2357 2909 3226 3837 3930 4004
Size: 436 241 299 552 317 611 93 74
Cuts at: 4004 4194 4256 4986 1381
Size: 190 62 730 2607
Fragments arranged by size:
2607 730 611 552 436 317 299 241
190 93 74 62
BspEI T'CCGG_A
Cuts at: 155 795 5432 155
Size: 640 4637 935
Fragments arranged by size:
4637 935 640
BspHI T'CATG_A
Cuts at: 5388 5388
Size: 6212
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1788 2078 2204 3262 3476 3778 4159 1788
Size: 290 126 1058 214 302 381 3841
Fragments arranged by size:
3841 1058 381 302 290 214 126
BsrBI CCG'CTC
Cuts at: 50 186 4504 4558 6042 6207 50
Size: 136 4318 54 1484 165 55
Fragments arranged by size:
4318 1484 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 4124 2009
Size: 2115 4097
BsrFI r'CCGG_y
Cuts at: 1385 4210 4391 4600 1385
Size: 2825 181 209 2997
Fragments arranged by size:
2997 2825 209 181
BsrGI T'GTAC_A
Cuts at: 605 605
Size: 6212
BssSI C'ACGA_G
Cuts at: 2358 4483 4841 2358
Size: 2125 358 3729
Fragments arranged by size:
3729 2125 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2067 3589 2067
Size: 1522 4690
BstBI TT'CG_AA
Cuts at: 110 4573 110
Size: 4463 1749
BstYI r'GATC_y
Cuts at: 1 193 461 1968 3237 3704 3727 4062 4308
Size: 192 268 1507 1269 467 23 335 246
Cuts at: 4308 5320 5406 5418 5764 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1507 1269 1012 467 449 346 335 268
246 192 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 952 952
Size: 6212
BtgI C'CryG_G
Cuts at: 770 1942 3021 770
Size: 1172 1079 3961
Fragments arranged by size:
3961 1172 1079
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4162 4343 4506 4162
Size: 181 163 5868
Fragments arranged by size:
5868 181 163
BtsI GCAGTG_nn'
Cuts at: 1193 1451 1501 1559 3455 1193
Size: 258 50 58 1896 3950
Fragments arranged by size:
3950 1896 258 58 50
DraI TTT'AAA
Cuts at: 1107 3211 1107
Size: 2104 4108
DrdI GACnn_nn'nnGTC
Cuts at: 3918 4776 3918
Size: 858 5354
EaeI y'GGCC_r
Cuts at: 527 1383 2654 3797 3971 4362 4389 5734 527
Size: 856 1271 1143 174 391 27 1345 1005
Fragments arranged by size:
1345 1271 1143 1005 856 391 174 27
EagI C'GGCC_G
Cuts at: 3797 3797
Size: 6212
EarI CTCTTCn'nnn_
Cuts at: 415 1315 1798 2165 2578 2726 2986 3029 4235
Size: 900 483 367 413 148 260 43 1206
Cuts at: 4235 4445 415
Size: 210 2182
Fragments arranged by size:
2182 1206 900 483 413 367 260 210
148 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4420 4730 4876 6022 4420
Size: 310 146 1146 4610
Fragments arranged by size:
4610 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3224 3224
Size: 6212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 844 1504 1652 1712 1744 1910 2122 2392
Size: 575 660 148 60 32 166 212 270
Cuts at: 2392 2611 2620 3010 3208 4037 4469 5216 5484
Size: 219 9 390 198 829 432 747 268
Cuts at: 5484 269
Size: 997
Fragments arranged by size:
997 829 747 660 575 432 390 270
268 219 212 198 166 148 60 32
9
EcoO109I rG'GnC_Cy
Cuts at: 777 1932 2112 2193 2691 3351 777
Size: 1155 180 81 498 660 3638
Fragments arranged by size:
3638 1155 660 498 180 81
EcoRI G'AATT_C
Cuts at: 3216 3216
Size: 6212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 864 4231 864
Size: 3367 2845
FspI TGC'GCA
Cuts at: 3993 3993
Size: 6212
HaeII r_GCGC'y
Cuts at: 1433 3646 3894 4916 5895 1433
Size: 2213 248 1022 979 1750
Fragments arranged by size:
2213 1750 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 795 827 4054 4084 4086 4116 795
Size: 32 3227 30 2 30 2891
Fragments arranged by size:
3227 2891 32 30 30 2
HincII GTy'rAC
Cuts at: 1423 3251 1423
Size: 1828 4384
HindIII A'AGCT_T
Cuts at: 1634 1634
Size: 6212
KasI G'GCGC_C
Cuts at: 3642 3890 3642
Size: 248 5964
KpnI G_GTAC'C
Cuts at: 3232 3232
Size: 6212
MfeI C'AATT_G
Cuts at: 601 601
Size: 6212
MluI A'CGCG_T
Cuts at: 5645 5645
Size: 6212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1728 2147 2463 4883 5067 1728
Size: 419 316 2420 184 2873
Fragments arranged by size:
2873 2420 419 316 184
MscI TGG'CCA
Cuts at: 529 3973 529
Size: 3444 2768
MslI CAynn'nnrTG
Cuts at: 487 656 2644 4201 4328 487
Size: 169 1988 1557 127 2371
Fragments arranged by size:
2371 1988 1557 169 127
MspA1I CmG'CkG
Cuts at: 351 976 2064 2685 2763 3323 3637 3997 5010
Size: 625 1088 621 78 560 314 360 1013
Cuts at: 5010 5255 351
Size: 245 1308
Fragments arranged by size:
1308 1088 1013 625 621 560 360 314
245 78
NaeI GCC'GGC
Cuts at: 4393 4393
Size: 6212
NarI GG'CG_CC
Cuts at: 3643 3891 3643
Size: 248 5964
NcoI C'CATG_G
Cuts at: 770 1942 770
Size: 1172 5040
NgoMIV G'CCGG_C
Cuts at: 4391 4391
Size: 6212
NsiI A_TGCA'T
Cuts at: 131 131
Size: 6212
NspI r_CATG'y
Cuts at: 255 486 3265 3765 4672 255
Size: 231 2779 500 907 1795
Fragments arranged by size:
2779 1795 907 500 231
PasI CC'CwG_GG
Cuts at: 1005 1543 1005
Size: 538 5674
PciI A'CATG_T
Cuts at: 251 4668 251
Size: 4417 1795
PflMI CCAn_nnn'nTGG
Cuts at: 867 1978 2799 867
Size: 1111 821 4280
Fragments arranged by size:
4280 1111 821
PfoI T'CCnGG_A
Cuts at: 1520 1520
Size: 6212
PmeI GTTT'AAAC
Cuts at: 3211 3211
Size: 6212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5351 5383 5351
Size: 32 6180
PpuMI rG'GwC_Cy
Cuts at: 1932 2112 2193 2691 1932
Size: 180 81 498 5453
Fragments arranged by size:
5453 498 180 81
PsiI TTA'TAA
Cuts at: 302 302
Size: 6212
PspXI vC'TCGA_Gb
Cuts at: 980 980
Size: 6212
PstI C_TGCA'G
Cuts at: 356 497 1002 1248 2063 2072 2744 3259 3944
Size: 141 505 246 815 9 672 515 685
Cuts at: 3944 356
Size: 2624
Fragments arranged by size:
2624 815 685 672 515 505 246 141
9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6212
PvuII CAG'CTG
Cuts at: 351 976 2064 2685 2763 3637 3997 351
Size: 625 1088 621 78 874 360 2566
Fragments arranged by size:
2566 1088 874 625 621 360 78
RsrII CG'GwC_CG
Cuts at: 4407 4407
Size: 6212
SacI G_AGCT'C
Cuts at: 3226 3226
Size: 6212
SalI G'TCGA_C
Cuts at: 3249 3249
Size: 6212
SapI GCTCTTCn'nnn_
Cuts at: 1315 2726 2986 4235 4445 1315
Size: 1411 260 1249 210 3082
Fragments arranged by size:
3082 1411 1249 260 210
SbfI CC_TGCA'GG
Cuts at: 3259 3259
Size: 6212
ScaI AGT'ACT
Cuts at: 960 5729 960
Size: 4769 1443
SfcI C'TryA_G
Cuts at: 33 352 493 536 998 1244 2059 2068 2740
Size: 319 141 43 462 246 815 9 672
Cuts at: 2740 3255 3940 4933 5124 33
Size: 515 685 993 191 1121
Fragments arranged by size:
1121 993 815 685 672 515 462 319
246 191 141 43 9
SfoI GGC'GCC
Cuts at: 3644 3892 3644
Size: 248 5964
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6212
SmaI CCC'GGG
Cuts at: 3234 3234
Size: 6212
SmlI C'TyrA_G
Cuts at: 595 980 1399 1910 2116 2284 3367 4282 4774
Size: 385 419 511 206 168 1083 915 492
Cuts at: 4774 5036 595
Size: 262 1771
Fragments arranged by size:
1771 1083 915 511 492 419 385 262
206 168
SnaBI TAC'GTA
Cuts at: 3204 3204
Size: 6212
SpeI A'CTAG_T
Cuts at: 67 1275 67
Size: 1208 5004
SphI G_CATG'C
Cuts at: 3265 3765 3265
Size: 500 5712
StuI AGG'CCT
Cuts at: 1687 2229 1687
Size: 542 5670
StyI C'CwwG_G
Cuts at: 770 1313 1942 3346 3700 770
Size: 543 629 1404 354 3282
Fragments arranged by size:
3282 1404 629 543 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 859 874 1837 2036 2242 3795 3928 4387 4596
Size: 15 963 199 206 1553 133 459 209
Cuts at: 4596 859
Size: 2475
Fragments arranged by size:
2475 1553 963 459 209 206 199 133
15
TatI w'GTAC_w
Cuts at: 605 958 1646 2075 5727 605
Size: 353 688 429 3652 1090
Fragments arranged by size:
3652 1090 688 429 353
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 241 273 2561 2593 5729 5761 241
Size: 32 2288 32 3136 32 692
Fragments arranged by size:
3136 2288 692 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1950 4009 1950
Size: 2059 4153
XbaI T'CTAG_A
Cuts at: 897 3243 897
Size: 2346 3866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3707 3707
Size: 6212
XhoI C'TCGA_G
Cuts at: 980 1910 980
Size: 930 5282
XmaI C'CCGG_G
Cuts at: 3232 3232
Size: 6212
XmnI GAAnn'nnTTC
Cuts at: 2434 2434
Size: 6212
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BmgBI BmtI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI PacI PmlI
PshAI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI SwaI ZraI