Restriction map for
pF1KSDA1710
(Circular) (Six-base) MAPSORT of: pF1KSDA1710.seq Check: 3408 from: 1 to: 4547
pF1KSDA1710 4547 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1585 1585
Size: 4547
Acc65I G'GTAC_C
Cuts at: 1563 1563
Size: 4547
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 549 633 885 969 1053 1221 2372 2804 3551
Size: 84 252 84 84 168 1151 432 747
Cuts at: 3551 3819 549
Size: 268 1277
Fragments arranged by size:
1277 1151 747 432 268 252 168 84
84 84
AflIII A'CryG_T
Cuts at: 1899 3003 3980 1899
Size: 1104 977 2466
Fragments arranged by size:
2466 1104 977
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AleI CACnn'nnGTG
Cuts at: 860 944 860
Size: 84 4463
AlwNI CAG_nnn'CTG
Cuts at: 493 1852 3419 493
Size: 1359 1567 1621
Fragments arranged by size:
1621 1567 1359
ApaLI G'TGCA_C
Cuts at: 3317 3317
Size: 4547
ApoI r'AATT_y
Cuts at: 1149 1271 1551 3900 4156 1149
Size: 122 280 2349 256 1540
Fragments arranged by size:
2349 1540 280 256 122
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 197 1567 197
Size: 1370 3177
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 159 1563 1977 2225 2260 159
Size: 1404 414 248 35 2446
Fragments arranged by size:
2446 1404 414 248 35
BanII G_rGCy'C
Cuts at: 913 1107 1249 1417 1501 1561 2591 913
Size: 194 142 168 84 60 1030 2869
Fragments arranged by size:
2869 1030 194 168 142 84 60
BbeI G_GCGC'C
Cuts at: 1981 2229 1981
Size: 248 4299
BbsI GAAGACnn'nnnn_
Cuts at: 604 604
Size: 4547
BbvCI CC'TCA_GC
Cuts at: 383 383
Size: 4547
BciVI GTATCCnnnnn_n'
Cuts at: 2445 3206 4547 2445
Size: 761 1341 2445
Fragments arranged by size:
2445 1341 761
BclI T'GATC_A
Cuts at: 2067 2067
Size: 4547
BglII A'GATC_T
Cuts at: 572 994 1335 2062 4099 572
Size: 422 341 727 2037 1020
Fragments arranged by size:
2037 1020 727 422 341
BlpI GC'TnA_GC
Cuts at: 923 1500 1659 923
Size: 577 159 3811
Fragments arranged by size:
3811 577 159
Bme1580I G_kGCm'C
Cuts at: 2172 2265 3321 2172
Size: 93 1056 3398
Fragments arranged by size:
3398 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 701 1759 1840 2175 4319 701
Size: 1058 81 335 2144 929
Fragments arranged by size:
2144 1058 929 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1520 2914 1520
Size: 1394 3153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 797 1217 1301 629
Size: 168 420 84 3875
Fragments arranged by size:
3875 420 168 84
Bpu10I CC'TnA_GC
Cuts at: 383 383
Size: 4547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1723 2602 3094 3392 1723
Size: 879 492 298 2878
Fragments arranged by size:
2878 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 204 4251 204
Size: 4047 500
BsaAI yAC'GTr
Cuts at: 1539 1900 1539
Size: 361 4186
BsaBI GATnn'nnATC
Cuts at: 6 2061 4098 6
Size: 2055 2037 455
Fragments arranged by size:
2055 2037 455
BsaHI Gr'CG_yC
Cuts at: 1978 2226 2928 4424 1978
Size: 248 702 1496 2101
Fragments arranged by size:
2101 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1736 2257 2935 3209 3356 3767 1736
Size: 521 678 274 147 411 2516
Fragments arranged by size:
2516 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 898 898
Size: 4547
BseYI C'CCAG_C
Cuts at: 148 1972 2730 3307 3803 148
Size: 1824 758 577 496 892
Fragments arranged by size:
1824 892 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 1408 209
Size: 1199 3348
BsiEI CG_ry'CG
Cuts at: 104 2089 2135 3343 104
Size: 1985 46 1208 1308
Fragments arranged by size:
1985 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 913 1249 1417 1501 1561 2339 2529 3321 913
Size: 336 168 84 60 778 190 792 2139
Fragments arranged by size:
2139 792 778 336 190 168 84 60
BsmI GAATG_Cn'
Cuts at: 384 1219 384
Size: 835 3712
BsmBI CGTCTCn'nnnn_
Cuts at: 829 829
Size: 4547
Bsp1286I G_dGCh'C
Cuts at: 913 1107 1249 1417 1501 1561 2172 2265 2339
Size: 194 142 168 84 60 611 93 74
Cuts at: 2339 2529 2591 3321 913
Size: 190 62 730 2139
Fragments arranged by size:
2139 730 611 194 190 168 142 93
84 74 62 60
BspEI T'CCGG_A
Cuts at: 3767 3767
Size: 4547
BspHI T'CATG_A
Cuts at: 3723 3723
Size: 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 378 1597 1811 2113 2494 378
Size: 1219 214 302 381 2431
Fragments arranged by size:
2431 1219 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2839 2893 4377 4542 50
Size: 2789 54 1484 165 55
Fragments arranged by size:
2789 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1224 2459 1224
Size: 1235 3312
BsrFI r'CCGG_y
Cuts at: 2545 2726 2935 2545
Size: 181 209 4157
Fragments arranged by size:
4157 209 181
BsrGI T'GTAC_A
Cuts at: 342 342
Size: 4547
BssSI C'ACGA_G
Cuts at: 2818 3176 2818
Size: 358 4189
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 1924
Size: 4547
BstBI TT'CG_AA
Cuts at: 110 2908 110
Size: 2798 1749
BstYI r'GATC_y
Cuts at: 1 572 994 1335 1572 2039 2062 2397 2643
Size: 571 422 341 237 467 23 335 246
Cuts at: 2643 3655 3741 3753 4099 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 571 467 449 422 346 341 335
246 237 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1081 2497 2678 2841 1081
Size: 1416 181 163 2787
Fragments arranged by size:
2787 1416 181 163
BtsI GCAGTG_nn'
Cuts at: 1396 1790 1396
Size: 394 4153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1206 1241 1206
Size: 35 4512
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3111 2253
Size: 858 3689
EaeI y'GGCC_r
Cuts at: 182 975 2132 2306 2697 2724 4069 182
Size: 793 1157 174 391 27 1345 660
Fragments arranged by size:
1345 1157 793 660 391 174 27
EagI C'GGCC_G
Cuts at: 2132 2132
Size: 4547
EarI CTCTTCn'nnn_
Cuts at: 398 455 1477 2570 2780 398
Size: 57 1022 1093 210 2165
Fragments arranged by size:
2165 1093 1022 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2755 3065 3211 4357 2755
Size: 310 146 1146 2945
Fragments arranged by size:
2945 1146 310 146
EcoICRI GAG'CTC
Cuts at: 911 1247 1415 1499 1559 911
Size: 336 168 84 60 3899
Fragments arranged by size:
3899 336 168 84 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 549 629 633 797 885 969 1053 1217 1221
Size: 80 4 164 88 84 84 164 4
Cuts at: 1221 1301 2372 2804 3551 3819 549
Size: 80 1071 432 747 268 1277
Fragments arranged by size:
1277 1071 747 432 268 164 164 88
84 84 80 80 4 4
EcoO109I rG'GnC_Cy
Cuts at: 152 234 851 935 1037 1686 152
Size: 82 617 84 102 649 3013
Fragments arranged by size:
3013 649 617 102 84 82
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4547
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 1981 2229 3251 4230 1981
Size: 248 1022 979 2298
Fragments arranged by size:
2298 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 744 776 984 986 1016 1018 1236 1268 2389
Size: 32 208 2 30 2 218 32 1121
Cuts at: 2389 2419 2421 2451 744
Size: 30 2 30 2840
Fragments arranged by size:
2840 1121 218 208 32 32 30 30
30 2 2 2
HincII GTy'rAC
Cuts at: 1586 1586
Size: 4547
HindIII A'AGCT_T
Cuts at: 897 1233 1317 897
Size: 336 84 4127
Fragments arranged by size:
4127 336 84
KasI G'GCGC_C
Cuts at: 1977 2225 1977
Size: 248 4299
KpnI G_GTAC'C
Cuts at: 1567 1567
Size: 4547
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 507 864 1106 1442 3218 3402 507
Size: 357 242 336 1776 184 1652
Fragments arranged by size:
1776 1652 357 336 242 184
MscI TGG'CCA
Cuts at: 184 2308 184
Size: 2124 2423
MslI CAynn'nnrTG
Cuts at: 860 944 1212 2536 2663 860
Size: 84 268 1324 127 2744
Fragments arranged by size:
2744 1324 268 127 84
MspA1I CmG'CkG
Cuts at: 1658 1972 2332 3345 3590 1658
Size: 314 360 1013 245 2615
Fragments arranged by size:
2615 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2728 2728
Size: 4547
NarI GG'CG_CC
Cuts at: 1978 2226 1978
Size: 248 4299
NcoI C'CATG_G
Cuts at: 125 125
Size: 4547
NdeI CA'TA_TG
Cuts at: 1209 1209
Size: 4547
NgoMIV G'CCGG_C
Cuts at: 2726 2726
Size: 4547
NspI r_CATG'y
Cuts at: 1600 2100 3007 1600
Size: 500 907 3140
Fragments arranged by size:
3140 907 500
PciI A'CATG_T
Cuts at: 3003 3003
Size: 4547
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4515
PpuMI rG'GwC_Cy
Cuts at: 152 234 851 935 152
Size: 82 617 84 3764
Fragments arranged by size:
3764 617 84 82
PstI C_TGCA'G
Cuts at: 1594 2279 1594
Size: 685 3862
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 1972 2332 1972
Size: 360 4187
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 913 1249 1417 1501 1561 913
Size: 336 168 84 60 3899
Fragments arranged by size:
3899 336 168 84 60
SalI G'TCGA_C
Cuts at: 1584 1584
Size: 4547
SanDI GG'GwC_CC
Cuts at: 152 152
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 2570 2780 2570
Size: 210 4337
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 4064 4064
Size: 4547
SfcI C'TryA_G
Cuts at: 33 1590 2275 3268 3459 33
Size: 1557 685 993 191 1121
Fragments arranged by size:
1557 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1979 2227 1979
Size: 248 4299
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SmaI CCC'GGG
Cuts at: 199 1569 199
Size: 1370 3177
SmlI C'TyrA_G
Cuts at: 1702 2617 3109 3371 1702
Size: 915 492 262 2878
Fragments arranged by size:
2878 915 492 262
SnaBI TAC'GTA
Cuts at: 1539 1539
Size: 4547
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1600 2100 1600
Size: 500 4047
StuI AGG'CCT
Cuts at: 562 702 562
Size: 140 4407
StyI C'CwwG_G
Cuts at: 125 1681 2035 125
Size: 1556 354 2637
Fragments arranged by size:
2637 1556 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1423 2130 2263 2722 2931 1423
Size: 707 133 459 209 3039
Fragments arranged by size:
3039 707 459 209 133
TatI w'GTAC_w
Cuts at: 342 4062 342
Size: 3720 827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 843 875 4064 4096 843
Size: 32 3189 32 1294
Fragments arranged by size:
3189 1294 32 32
Tth111I GACn'n_nGTC
Cuts at: 2344 2344
Size: 4547
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 2042 141
Size: 1901 2646
XmaI C'CCGG_G
Cuts at: 197 1567 197
Size: 1370 3177
XmnI GAAnn'nnTTC
Cuts at: 741 898 1066 1234 1402 1486 741
Size: 157 168 168 168 84 3802
Fragments arranged by size:
3802 168 168 168 157 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SrfI SspI SwaI XhoI ZraI