Restriction map for
pF1KSDA1712
(Circular) (Six-base) MAPSORT of: pF1KSDA1712.seq Check: 8885 from: 1 to: 4307
pF1KSDA1712 4307 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1345 1345
Size: 4307
Acc65I G'GTAC_C
Cuts at: 1323 1323
Size: 4307
AclI AA'CG_TT
Cuts at: 4147 4147
Size: 4307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 487 788 2132 2564 3311 3579 215
Size: 272 301 1344 432 747 268 943
Fragments arranged by size:
1344 943 747 432 301 272 268
AflII C'TTAA_G
Cuts at: 868 868
Size: 4307
AflIII A'CryG_T
Cuts at: 1659 2763 3740 1659
Size: 1104 977 2226
Fragments arranged by size:
2226 1104 977
AgeI A'CCGG_T
Cuts at: 2695 2695
Size: 4307
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 158 190 158
Size: 32 4275
AlwNI CAG_nnn'CTG
Cuts at: 589 1612 3179 589
Size: 1023 1567 1717
Fragments arranged by size:
1717 1567 1023
ApaLI G'TGCA_C
Cuts at: 3077 3077
Size: 4307
ApoI r'AATT_y
Cuts at: 1018 1311 3660 3916 1018
Size: 293 2349 256 1409
Fragments arranged by size:
2349 1409 293 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4307
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
AvaI C'yCGr_G
Cuts at: 1327 1327
Size: 4307
BamHI G'GATC_C
Cuts at: 1332 1332
Size: 4307
BanI G'GyrC_C
Cuts at: 1323 1737 1985 2020 1323
Size: 414 248 35 3610
Fragments arranged by size:
3610 414 248 35
BanII G_rGCy'C
Cuts at: 320 1321 2351 320
Size: 1001 1030 2276
Fragments arranged by size:
2276 1030 1001
BbeI G_GCGC'C
Cuts at: 1741 1989 1741
Size: 248 4059
BbsI GAAGACnn'nnnn_
Cuts at: 493 1052 493
Size: 559 3748
BciVI GTATCCnnnnn_n'
Cuts at: 684 2205 2966 4307 684
Size: 1521 761 1341 684
Fragments arranged by size:
1521 1341 761 684
BclI T'GATC_A
Cuts at: 372 1827 372
Size: 1455 2852
BglII A'GATC_T
Cuts at: 1822 3859 1822
Size: 2037 2270
BlpI GC'TnA_GC
Cuts at: 794 1419 794
Size: 625 3682
Bme1580I G_kGCm'C
Cuts at: 1932 2025 3081 1932
Size: 93 1056 3158
Fragments arranged by size:
3158 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1519 1600 1935 4079 1519
Size: 81 335 2144 1747
Fragments arranged by size:
2144 1747 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 2362 2854 3152 1483
Size: 879 492 298 2638
Fragments arranged by size:
2638 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 221 4011 221
Size: 3790 517
BsaAI yAC'GTr
Cuts at: 1299 1660 1299
Size: 361 3946
BsaBI GATnn'nnATC
Cuts at: 6 1821 3858 6
Size: 1815 2037 455
Fragments arranged by size:
2037 1815 455
BsaHI Gr'CG_yC
Cuts at: 1738 1986 2688 4184 1738
Size: 248 702 1496 1861
Fragments arranged by size:
1861 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1496 2017 2695 2969 3116 3527 1496
Size: 521 678 274 147 411 2276
Fragments arranged by size:
2276 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 158 188 158
Size: 30 4277
BseYI C'CCAG_C
Cuts at: 231 1732 2490 3067 3563 231
Size: 1501 758 577 496 975
Fragments arranged by size:
1501 975 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 815
Size: 4307
BsiEI CG_ry'CG
Cuts at: 104 1849 1895 3103 104
Size: 1745 46 1208 1308
Fragments arranged by size:
1745 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 176 320 1321 2099 2289 3081 176
Size: 144 1001 778 190 792 1402
Fragments arranged by size:
1402 1001 792 778 190 144
BsmI GAATG_Cn'
Cuts at: 859 859
Size: 4307
Bsp1286I G_dGCh'C
Cuts at: 176 320 1321 1932 2025 2099 2289 2351 3081
Size: 144 1001 611 93 74 190 62 730
Cuts at: 3081 176
Size: 1402
Fragments arranged by size:
1402 1001 730 611 190 144 93 74
62
BspEI T'CCGG_A
Cuts at: 3527 3527
Size: 4307
BspHI T'CATG_A
Cuts at: 3483 3483
Size: 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 602 1357 1571 1873 2254 602
Size: 755 214 302 381 2655
Fragments arranged by size:
2655 755 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2599 2653 4137 4302 50
Size: 2549 54 1484 165 55
Fragments arranged by size:
2549 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 573 2219 573
Size: 1646 2661
BsrFI r'CCGG_y
Cuts at: 2305 2486 2695 2305
Size: 181 209 3917
Fragments arranged by size:
3917 209 181
BssSI C'ACGA_G
Cuts at: 2578 2936 2578
Size: 358 3949
BstAPI GCAn_nnn'nTGC
Cuts at: 1684 1684
Size: 4307
BstBI TT'CG_AA
Cuts at: 110 2668 110
Size: 2558 1749
BstYI r'GATC_y
Cuts at: 1 1332 1799 1822 2157 2403 3415 3501 3513
Size: 1331 467 23 335 246 1012 86 12
Cuts at: 3513 3859 1
Size: 346 449
Fragments arranged by size:
1331 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 624 624
Size: 4307
BtgI C'CryG_G
Cuts at: 125 125
Size: 4307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2257 2438 2601 2257
Size: 181 163 3963
Fragments arranged by size:
3963 181 163
BtsI GCAGTG_nn'
Cuts at: 1550 1550
Size: 4307
DraI TTT'AAA
Cuts at: 1306 1306
Size: 4307
DrdI GACnn_nn'nnGTC
Cuts at: 2013 2871 2013
Size: 858 3449
EaeI y'GGCC_r
Cuts at: 1892 2066 2457 2484 3829 1892
Size: 174 391 27 1345 2370
Fragments arranged by size:
2370 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1892 1892
Size: 4307
EarI CTCTTCn'nnn_
Cuts at: 191 983 2330 2540 191
Size: 792 1347 210 1958
Fragments arranged by size:
1958 1347 792 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2515 2825 2971 4117 2515
Size: 310 146 1146 2705
Fragments arranged by size:
2705 1146 310 146
EcoICRI GAG'CTC
Cuts at: 318 1319 318
Size: 1001 3306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 487 788 2132 2564 3311 3579 215
Size: 272 301 1344 432 747 268 943
Fragments arranged by size:
1344 943 747 432 301 272 268
EcoNI CCTnn'n_nnAGG
Cuts at: 166 166
Size: 4307
EcoO109I rG'GnC_Cy
Cuts at: 1446 1446
Size: 4307
EcoRI G'AATT_C
Cuts at: 1311 1311
Size: 4307
EcoRV GAT'ATC
Cuts at: 603 603
Size: 4307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 883 2326 883
Size: 1443 2864
FspI TGC'GCA
Cuts at: 2088 2088
Size: 4307
HaeII r_GCGC'y
Cuts at: 1741 1989 3011 3990 1741
Size: 248 1022 979 2058
Fragments arranged by size:
2058 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 2149 2179 2181 2211 562
Size: 32 1555 30 2 30 2658
Fragments arranged by size:
2658 1555 32 30 30 2
HincII GTy'rAC
Cuts at: 678 1346 678
Size: 668 3639
HindIII A'AGCT_T
Cuts at: 150 361 150
Size: 211 4096
KasI G'GCGC_C
Cuts at: 1737 1985 1737
Size: 248 4059
KpnI G_GTAC'C
Cuts at: 1327 1327
Size: 4307
MluI A'CGCG_T
Cuts at: 3740 3740
Size: 4307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2978 3162 2978
Size: 184 4123
MscI TGG'CCA
Cuts at: 2068 2068
Size: 4307
MslI CAynn'nnrTG
Cuts at: 2296 2423 2296
Size: 127 4180
MspA1I CmG'CkG
Cuts at: 1418 1732 2092 3105 3350 1418
Size: 314 360 1013 245 2375
Fragments arranged by size:
2375 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2488 2488
Size: 4307
NarI GG'CG_CC
Cuts at: 1738 1986 1738
Size: 248 4059
NcoI C'CATG_G
Cuts at: 125 125
Size: 4307
NgoMIV G'CCGG_C
Cuts at: 2486 2486
Size: 4307
NspI r_CATG'y
Cuts at: 1360 1860 2767 1360
Size: 500 907 2900
Fragments arranged by size:
2900 907 500
PciI A'CATG_T
Cuts at: 2763 2763
Size: 4307
PflMI CCAn_nnn'nTGG
Cuts at: 1230 1230
Size: 4307
PmeI GTTT'AAAC
Cuts at: 1306 1306
Size: 4307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 158 190 3446 3478 158
Size: 32 3256 32 987
Fragments arranged by size:
3256 987 32 32
PsiI TTA'TAA
Cuts at: 298 386 298
Size: 88 4219
PstI C_TGCA'G
Cuts at: 585 1266 1354 2039 585
Size: 681 88 685 2853
Fragments arranged by size:
2853 685 681 88
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4307
PvuII CAG'CTG
Cuts at: 1732 2092 1732
Size: 360 3947
RsrII CG'GwC_CG
Cuts at: 2502 2502
Size: 4307
SacI G_AGCT'C
Cuts at: 320 1321 320
Size: 1001 3306
SalI G'TCGA_C
Cuts at: 1344 1344
Size: 4307
SapI GCTCTTCn'nnn_
Cuts at: 2330 2540 2330
Size: 210 4097
SbfI CC_TGCA'GG
Cuts at: 1354 1354
Size: 4307
ScaI AGT'ACT
Cuts at: 3824 3824
Size: 4307
SfcI C'TryA_G
Cuts at: 33 581 1137 1262 1350 2035 3028 3219 33
Size: 548 556 125 88 685 993 191 1121
Fragments arranged by size:
1121 993 685 556 548 191 125 88
SfoI GGC'GCC
Cuts at: 1739 1987 1739
Size: 248 4059
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
SmaI CCC'GGG
Cuts at: 1329 1329
Size: 4307
SmlI C'TyrA_G
Cuts at: 868 1462 2377 2869 3131 868
Size: 594 915 492 262 2044
Fragments arranged by size:
2044 915 594 492 262
SnaBI TAC'GTA
Cuts at: 1299 1299
Size: 4307
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4307
SphI G_CATG'C
Cuts at: 1360 1860 1360
Size: 500 3807
StyI C'CwwG_G
Cuts at: 125 1441 1795 125
Size: 1316 354 2637
Fragments arranged by size:
2637 1316 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1890 2023 2482 2691 1890
Size: 133 459 209 3506
Fragments arranged by size:
3506 459 209 133
TatI w'GTAC_w
Cuts at: 1141 3822 1141
Size: 2681 1626
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4275
Tth111I GACn'n_nGTC
Cuts at: 2104 2104
Size: 4307
XbaI T'CTAG_A
Cuts at: 1338 1338
Size: 4307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1802 1802
Size: 4307
XmaI C'CCGG_G
Cuts at: 1327 1327
Size: 4307
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI