Restriction map for
pF1KSDA1719
(Circular) (Six-base) MAPSORT of: pF1KSDA1719.seq Check: 2297 from: 1 to: 6266
pF1KSDA1719 6266 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1266 1623 2286 1266
Size: 357 663 5246
Fragments arranged by size:
5246 663 357
AatII G_ACGT'C
Cuts at: 572 572
Size: 6266
AccI GT'mk_AC
Cuts at: 3304 3304
Size: 6266
Acc65I G'GTAC_C
Cuts at: 3282 3282
Size: 6266
AclI AA'CG_TT
Cuts at: 2249 6106 2249
Size: 3857 2409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 2050 2122 2341 4091 4523 5270 5538 724
Size: 1326 72 219 1750 432 747 268 1452
Fragments arranged by size:
1750 1452 1326 747 432 268 219 72
AfeI AGC'GCT
Cuts at: 2107 2107
Size: 6266
AflIII A'CryG_T
Cuts at: 3618 4722 5699 3618
Size: 1104 977 4185
Fragments arranged by size:
4185 1104 977
AgeI A'CCGG_T
Cuts at: 4654 4654
Size: 6266
AhdI GACnn_n'nnGTC
Cuts at: 2766 2766
Size: 6266
AleI CACnn'nnGTG
Cuts at: 268 268
Size: 6266
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1735 1767 1735
Size: 32 6234
AlwNI CAG_nnn'CTG
Cuts at: 895 1707 2707 3571 5138 895
Size: 812 1000 864 1567 2023
Fragments arranged by size:
2023 1567 1000 864 812
ApaI G_GGCC'C
Cuts at: 176 2521 3219 176
Size: 2345 698 3223
Fragments arranged by size:
3223 2345 698
ApaLI G'TGCA_C
Cuts at: 1474 5036 1474
Size: 3562 2704
ApoI r'AATT_y
Cuts at: 3270 5619 5875 3270
Size: 2349 256 3661
Fragments arranged by size:
3661 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6266
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6266
AvaI C'yCGr_G
Cuts at: 2549 2717 2842 3286 2549
Size: 168 125 444 5529
Fragments arranged by size:
5529 444 168 125
BamHI G'GATC_C
Cuts at: 428 1360 3291 428
Size: 932 1931 3403
Fragments arranged by size:
3403 1931 932
BanI G'GyrC_C
Cuts at: 322 619 1030 1786 1918 2077 2209 2821 3138
Size: 297 411 756 132 159 132 612 317
Cuts at: 3138 3282 3696 3944 3979 322
Size: 144 414 248 35 2609
Fragments arranged by size:
2609 756 612 414 411 317 297 248
159 144 132 132 35
BanII G_rGCy'C
Cuts at: 140 176 700 996 1429 1871 2521 2615 2701
Size: 36 524 296 433 442 650 94 86
Cuts at: 2701 3219 3280 4310 140
Size: 518 61 1030 2096
Fragments arranged by size:
2096 1030 650 524 518 442 433 296
94 86 61 36
BbeI G_GCGC'C
Cuts at: 3700 3948 3700
Size: 248 6018
BbsI GAAGACnn'nnnn_
Cuts at: 1280 2765 1280
Size: 1485 4781
BbvCI CC'TCA_GC
Cuts at: 1516 2587 2791 1516
Size: 1071 204 4991
Fragments arranged by size:
4991 1071 204
BciVI GTATCCnnnnn_n'
Cuts at: 4164 4925 6266 4164
Size: 761 1341 4164
Fragments arranged by size:
4164 1341 761
BclI T'GATC_A
Cuts at: 278 1874 3176 3786 278
Size: 1596 1302 610 2758
Fragments arranged by size:
2758 1596 1302 610
BglI GCCn_nnn'nGGC
Cuts at: 1140 1464 1723 2261 2583 1140
Size: 324 259 538 322 4823
Fragments arranged by size:
4823 538 324 322 259
BglII A'GATC_T
Cuts at: 3781 5818 3781
Size: 2037 4229
BlpI GC'TnA_GC
Cuts at: 139 724 2272 3378 139
Size: 585 1548 1106 3027
Fragments arranged by size:
3027 1548 1106 585
Bme1580I G_kGCm'C
Cuts at: 176 624 638 1121 1205 1478 1921 2143 2459
Size: 448 14 483 84 273 443 222 316
Cuts at: 2459 2521 2557 2824 3219 3891 3984 5040 176
Size: 62 36 267 395 672 93 1056 1402
Fragments arranged by size:
1402 1056 672 483 448 443 395 316
273 267 222 93 84 62 36 14
BmgBI CAC'GTC
Cuts at: 3105 3105
Size: 6266
BmrI ACTGGGnnnn_n'
Cuts at: 2453 3478 3559 3894 6038 2453
Size: 1025 81 335 2144 2681
Fragments arranged by size:
2681 2144 1025 335 81
BmtI G_CTAG'C
Cuts at: 1441 1441
Size: 6266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1531 2070 2770 4633 1531
Size: 539 700 1863 3164
Fragments arranged by size:
3164 1863 700 539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 520 1025 1123 1428 1516 1522 1762 1945
Size: 344 505 98 305 88 6 240 183
Cuts at: 1945 2086 2324 2785 2821 3055 176
Size: 141 238 461 36 234 3387
Fragments arranged by size:
3387 505 461 344 305 240 238 234
183 141 98 88 36 6
Bpu10I CC'TnA_GC
Cuts at: 1516 2587 2791 1516
Size: 1071 204 4991
Fragments arranged by size:
4991 1071 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1078 2242 3442 4321 4813 5111 457
Size: 621 1164 1200 879 492 298 1612
Fragments arranged by size:
1612 1200 1164 879 621 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 142 354 1001 1477 1502 1555 2062 2302 2374
Size: 212 647 476 25 53 507 240 72
Cuts at: 2374 5970 142
Size: 3596 438
Fragments arranged by size:
3596 647 507 476 438 240 212 72
53 25
BsaAI yAC'GTr
Cuts at: 666 1797 2220 3258 3619 666
Size: 1131 423 1038 361 3313
Fragments arranged by size:
3313 1131 1038 423 361
BsaBI GATnn'nnATC
Cuts at: 6 3780 5817 6
Size: 3774 2037 455
Fragments arranged by size:
3774 2037 455
BsaHI Gr'CG_yC
Cuts at: 569 886 1715 1988 3697 3945 4647 6143 569
Size: 317 829 273 1709 248 702 1496 692
Fragments arranged by size:
1709 1496 829 702 692 317 273 248
BsaWI w'CCGG_w
Cuts at: 929 2036 3455 3976 4654 4928 5075 5486 929
Size: 1107 1419 521 678 274 147 411 1709
Fragments arranged by size:
1709 1419 1107 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 493 523 1735 1765 2297 2327 2902 2932 493
Size: 30 1212 30 532 30 575 30 3827
Fragments arranged by size:
3827 1212 575 532 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 629 1182 1413 2677 2680 2683 2737 2926
Size: 367 553 231 1264 3 3 54 189
Cuts at: 2926 262
Size: 3602
Fragments arranged by size:
3602 1264 553 367 231 189 54 3
3
BseYI C'CCAG_C
Cuts at: 300 1187 1342 1364 1827 2403 2640 2700 3235
Size: 887 155 22 463 576 237 60 535
Cuts at: 3235 3691 4449 5026 5522 300
Size: 456 758 577 496 1044
Fragments arranged by size:
1044 887 758 577 576 535 496 463
456 237 155 60 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 810 982 1165 1183 2848 2941 730
Size: 80 172 183 18 1665 93 4055
Fragments arranged by size:
4055 1665 183 172 93 80 18
BsiEI CG_ry'CG
Cuts at: 104 957 2269 3226 3808 3854 5062 104
Size: 853 1312 957 582 46 1208 1308
Fragments arranged by size:
1312 1308 1208 957 853 582 46
BsiHKAI G_wGCw'C
Cuts at: 1429 1478 1502 3280 4058 4248 5040 1429
Size: 49 24 1778 778 190 792 2655
Fragments arranged by size:
2655 1778 792 778 190 49 24
BsmI GAATG_Cn'
Cuts at: 239 2231 239
Size: 1992 4274
BsmBI CGTCTCn'nnnn_
Cuts at: 576 3000 576
Size: 2424 3842
Bsp1286I G_dGCh'C
Cuts at: 140 176 624 638 700 996 1121 1205 1429
Size: 36 448 14 62 296 125 84 224
Cuts at: 1429 1478 1502 1871 1921 2143 2459 2521 2557
Size: 49 24 369 50 222 316 62 36
Cuts at: 2557 2615 2701 2824 3219 3280 3891 3984 4058
Size: 58 86 123 395 61 611 93 74
Cuts at: 4058 4248 4310 5040 140
Size: 190 62 730 1366
Fragments arranged by size:
1366 730 611 448 395 369 316 296
224 222 190 125 123 93 86 84
74 62 62 62 61 58 50 49
36 36 24 14
BspEI T'CCGG_A
Cuts at: 929 2036 5486 929
Size: 1107 3450 1709
Fragments arranged by size:
3450 1709 1107
BspHI T'CATG_A
Cuts at: 2813 5442 2813
Size: 2629 3637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1266 1623 2286 3084 3316 3530 3832 4213 1266
Size: 357 663 798 232 214 302 381 3319
Fragments arranged by size:
3319 798 663 381 357 302 232 214
BsrBI CCG'CTC
Cuts at: 50 529 989 2571 4558 4612 6096 6261 50
Size: 479 460 1582 1987 54 1484 165 55
Fragments arranged by size:
1987 1582 1484 479 460 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 4178 1664
Size: 2514 3752
BsrFI r'CCGG_y
Cuts at: 2264 2448 2861 4264 4445 4654 2264
Size: 184 413 1403 181 209 3876
Fragments arranged by size:
3876 1403 413 209 184 181
BssSI C'ACGA_G
Cuts at: 796 1582 4537 4895 796
Size: 786 2955 358 2167
Fragments arranged by size:
2955 2167 786 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1473 2001 3643 1473
Size: 528 1642 4096
Fragments arranged by size:
4096 1642 528
BstBI TT'CG_AA
Cuts at: 110 4627 110
Size: 4517 1749
BstEII G'GTnAC_C
Cuts at: 2949 2949
Size: 6266
BstXI CCAn_nnnn'nTGG
Cuts at: 631 771 978 1074 1452 2220 631
Size: 140 207 96 378 768 4677
Fragments arranged by size:
4677 768 378 207 140 96
BstYI r'GATC_y
Cuts at: 1 428 905 1106 1360 2033 3291 3758 3781
Size: 427 477 201 254 673 1258 467 23
Cuts at: 3781 4116 4362 5374 5460 5472 5818 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1258 1012 673 477 467 449 427 346
335 254 246 201 86 23 12
BtgI C'CryG_G
Cuts at: 1356 1487 1979 3060 1356
Size: 131 492 1081 4562
Fragments arranged by size:
4562 1081 492 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4216 4397 4560 4216
Size: 181 163 5922
Fragments arranged by size:
5922 181 163
BtsI GCAGTG_nn'
Cuts at: 793 1044 1160 1230 1788 2014 3509 793
Size: 251 116 70 558 226 1495 3550
Fragments arranged by size:
3550 1495 558 251 226 116 70
ClaI AT'CG_AT
Cuts at: 1025 1025
Size: 6266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 545 580 2848 2883 545
Size: 35 2268 35 3928
Fragments arranged by size:
3928 2268 35 35
DraI TTT'AAA
Cuts at: 3265 3265
Size: 6266
DraIII CAC_nnn'GTG
Cuts at: 2949 3036 2949
Size: 87 6179
DrdI GACnn_nn'nnGTC
Cuts at: 891 2164 3972 4830 891
Size: 1273 1808 858 2327
Fragments arranged by size:
2327 1808 1273 858
EaeI y'GGCC_r
Cuts at: 773 827 1076 1132 1227 1263 1847 1946 2237
Size: 54 249 56 95 36 584 99 291
Cuts at: 2237 2266 2495 2856 3149 3851 4025 4416 4443
Size: 29 229 361 293 702 174 391 27
Cuts at: 4443 5788 773
Size: 1345 1251
Fragments arranged by size:
1345 1251 702 584 391 361 293 291
249 229 174 99 95 56 54 36
29 27
EagI C'GGCC_G
Cuts at: 2266 3851 2266
Size: 1585 4681
EarI CTCTTCn'nnn_
Cuts at: 4289 4499 4289
Size: 210 6056
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 178 436 1410 1777 1951 4474 4784 4930 6076
Size: 258 974 367 174 2523 310 146 1146
Cuts at: 6076 178
Size: 368
Fragments arranged by size:
2523 1146 974 368 367 310 258 174
146
EcoICRI GAG'CTC
Cuts at: 1427 3278 1427
Size: 1851 4415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 520 724 1025 1123 1428 1516 1522 1762
Size: 344 204 301 98 305 88 6 240
Cuts at: 1762 1945 2050 2086 2122 2324 2341 2785 2821
Size: 183 105 36 36 202 17 444 36
Cuts at: 2821 3055 4091 4523 5270 5538 176
Size: 234 1036 432 747 268 904
Fragments arranged by size:
1036 904 747 444 432 344 305 301
268 240 234 204 202 183 105 98
88 36 36 36 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 580 2293 2959 3081 3151 580
Size: 1713 666 122 70 3695
Fragments arranged by size:
3695 1713 666 122 70
EcoO109I rG'GnC_Cy
Cuts at: 173 859 1142 1191 2016 2116 2400 2517 2525
Size: 686 283 49 825 100 284 117 8
Cuts at: 2525 2964 3087 3216 3232 3405 173
Size: 439 123 129 16 173 3034
Fragments arranged by size:
3034 825 686 439 284 283 173 129
123 117 100 49 16 8
EcoRI G'AATT_C
Cuts at: 3270 3270
Size: 6266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4285 4285
Size: 6266
FseI GG_CCGG'CC
Cuts at: 2268 2268
Size: 6266
FspI TGC'GCA
Cuts at: 4047 4047
Size: 6266
HaeII r_GCGC'y
Cuts at: 2109 3700 3948 4970 5949 2109
Size: 1591 248 1022 979 2426
Fragments arranged by size:
2426 1591 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 998 1030 1552 1584 2297 2329 2752 2784 4108
Size: 32 522 32 713 32 423 32 1324
Cuts at: 4108 4138 4140 4170 998
Size: 30 2 30 3094
Fragments arranged by size:
3094 1324 713 522 423 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3099 3305 3099
Size: 206 6060
KasI G'GCGC_C
Cuts at: 3696 3944 3696
Size: 248 6018
KpnI G_GTAC'C
Cuts at: 3286 3286
Size: 6266
MluI A'CGCG_T
Cuts at: 5699 5699
Size: 6266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 377 1907 4937 5121 377
Size: 1530 3030 184 1522
Fragments arranged by size:
3030 1530 1522 184
MscI TGG'CCA
Cuts at: 775 829 1078 1265 1948 2239 2858 4027 775
Size: 54 249 187 683 291 619 1169 3014
Fragments arranged by size:
3014 1169 683 619 291 249 187 54
MslI CAynn'nnrTG
Cuts at: 268 629 1036 2218 4255 4382 268
Size: 361 407 1182 2037 127 2152
Fragments arranged by size:
2152 2037 1182 407 361 127
MspA1I CmG'CkG
Cuts at: 744 1131 1179 1187 1520 2271 2644 3377 3691
Size: 387 48 8 333 751 373 733 314
Cuts at: 3691 4051 5064 5309 744
Size: 360 1013 245 1701
Fragments arranged by size:
1701 1013 751 733 387 373 360 333
314 245 48 8
NaeI GCC'GGC
Cuts at: 2266 2450 4447 2266
Size: 184 1997 4085
Fragments arranged by size:
4085 1997 184
NarI GG'CG_CC
Cuts at: 3697 3945 3697
Size: 248 6018
NcoI C'CATG_G
Cuts at: 1979 1979
Size: 6266
NgoMIV G'CCGG_C
Cuts at: 2264 2448 4445 2264
Size: 184 1997 4085
Fragments arranged by size:
4085 1997 184
NheI G'CTAG_C
Cuts at: 1437 1437
Size: 6266
NspI r_CATG'y
Cuts at: 2742 3319 3819 4726 2742
Size: 577 500 907 4282
Fragments arranged by size:
4282 907 577 500
PasI CC'CwG_GG
Cuts at: 344 900 2121 2521 344
Size: 556 1221 400 4089
Fragments arranged by size:
4089 1221 556 400
PciI A'CATG_T
Cuts at: 4722 4722
Size: 6266
PflMI CCAn_nnn'nTGG
Cuts at: 1783 2707 1783
Size: 924 5342
PfoI T'CCnGG_A
Cuts at: 3008 3008
Size: 6266
PmeI GTTT'AAAC
Cuts at: 3265 3265
Size: 6266
PmlI CAC'GTG
Cuts at: 1797 2220 1797
Size: 423 5843
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1735 1767 5405 5437 1735
Size: 32 3638 32 2564
Fragments arranged by size:
3638 2564 32 32
PpuMI rG'GwC_Cy
Cuts at: 859 1191 2016 2116 2400 2525 2964 3087 859
Size: 332 825 100 284 125 439 123 4038
Fragments arranged by size:
4038 825 439 332 284 125 123 100
PspOMI G'GGCC_C
Cuts at: 172 2517 3215 172
Size: 2345 698 3223
Fragments arranged by size:
3223 2345 698
PspXI vC'TCGA_Gb
Cuts at: 2842 2842
Size: 6266
PstI C_TGCA'G
Cuts at: 1237 1634 2297 2904 3095 3313 3998 1237
Size: 397 663 607 191 218 685 3505
Fragments arranged by size:
3505 685 663 607 397 218 191
PvuI CG_AT'CG
Cuts at: 104 3226 104
Size: 3122 3144
PvuII CAG'CTG
Cuts at: 1179 2644 3691 4051 1179
Size: 1465 1047 360 3394
Fragments arranged by size:
3394 1465 1047 360
RsrII CG'GwC_CG
Cuts at: 4461 4461
Size: 6266
SacI G_AGCT'C
Cuts at: 1429 3280 1429
Size: 1851 4415
SalI G'TCGA_C
Cuts at: 3303 3303
Size: 6266
SanDI GG'GwC_CC
Cuts at: 859 1191 2116 859
Size: 332 925 5009
Fragments arranged by size:
5009 925 332
SapI GCTCTTCn'nnn_
Cuts at: 4289 4499 4289
Size: 210 6056
SbfI CC_TGCA'GG
Cuts at: 2297 3095 3313 2297
Size: 798 218 5250
Fragments arranged by size:
5250 798 218
ScaI AGT'ACT
Cuts at: 5783 5783
Size: 6266
SexAI A'CCwGG_T
Cuts at: 2018 2018
Size: 6266
SfcI C'TryA_G
Cuts at: 33 1233 1630 2293 2900 3091 3309 3994 4987
Size: 1200 397 663 607 191 218 685 993
Cuts at: 4987 5178 33
Size: 191 1121
Fragments arranged by size:
1200 1121 993 685 663 607 397 218
191 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1140 1140
Size: 6266
SfoI GGC'GCC
Cuts at: 3698 3946 3698
Size: 248 6018
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6266
SmaI CCC'GGG
Cuts at: 2551 3288 2551
Size: 737 5529
SmlI C'TyrA_G
Cuts at: 472 1057 2257 2842 3421 4336 4828 5090 472
Size: 585 1200 585 579 915 492 262 1648
Fragments arranged by size:
1648 1200 915 585 585 579 492 262
SnaBI TAC'GTA
Cuts at: 3258 3258
Size: 6266
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6266
SphI G_CATG'C
Cuts at: 3319 3819 3319
Size: 500 5766
StuI AGG'CCT
Cuts at: 2177 2426 2577 2853 3068 2177
Size: 249 151 276 215 5375
Fragments arranged by size:
5375 276 249 215 151
StyI C'CwwG_G
Cuts at: 1979 2866 2933 3400 3754 1979
Size: 887 67 467 354 4491
Fragments arranged by size:
4491 887 467 354 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 404 2619 3849 3982 4441 4650 404
Size: 2215 1230 133 459 209 2020
Fragments arranged by size:
2215 2020 1230 459 209 133
TatI w'GTAC_w
Cuts at: 5781 5781
Size: 6266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1003 1035 1312 1344 5783 5815 1003
Size: 32 277 32 4439 32 1454
Fragments arranged by size:
4439 1454 277 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1566 1645 3085 4063 1566
Size: 79 1440 978 3769
Fragments arranged by size:
3769 1440 978 79
XbaI T'CTAG_A
Cuts at: 3297 3297
Size: 6266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1071 2190 3761 1071
Size: 1119 1571 3576
Fragments arranged by size:
3576 1571 1119
XhoI C'TCGA_G
Cuts at: 2842 2842
Size: 6266
XmaI C'CCGG_G
Cuts at: 2549 3286 2549
Size: 737 5529
ZraI GAC'GTC
Cuts at: 570 570
Size: 6266
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AlfI AscI AvrII BaeI BcgI BfrBI BsiWI
BsrGI BssHII BstZ17I Bsu36I EcoRV FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PshAI PsiI
PsrI SacII SgrAI SrfI SspI SwaI XmnI