Restriction map for
pF1KSDA1720
(Circular) (Six-base) MAPSORT of: pF1KSDA1720.seq Check: 426 from: 1 to: 4316
pF1KSDA1720 4316 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1354 1354
Size: 4316
Acc65I G'GTAC_C
Cuts at: 1332 1332
Size: 4316
AclI AA'CG_TT
Cuts at: 4156 4156
Size: 4316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 1136 2141 2573 3320 3588 206
Size: 930 1005 432 747 268 934
Fragments arranged by size:
1005 934 930 747 432 268
AfeI AGC'GCT
Cuts at: 527 527
Size: 4316
AflIII A'CryG_T
Cuts at: 1668 2772 3749 1668
Size: 1104 977 2235
Fragments arranged by size:
2235 1104 977
AgeI A'CCGG_T
Cuts at: 2704 2704
Size: 4316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 578 578
Size: 4316
AlwNI CAG_nnn'CTG
Cuts at: 831 1621 3188 831
Size: 790 1567 1959
Fragments arranged by size:
1959 1567 790
ApaI G_GGCC'C
Cuts at: 982 1097 982
Size: 115 4201
ApaLI G'TGCA_C
Cuts at: 3086 3086
Size: 4316
ApoI r'AATT_y
Cuts at: 1320 3669 3925 1320
Size: 2349 256 1711
Fragments arranged by size:
2349 1711 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4316
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
AvaI C'yCGr_G
Cuts at: 566 743 1018 1336 566
Size: 177 275 318 3546
Fragments arranged by size:
3546 318 275 177
AvrII C'CTAG_G
Cuts at: 208 208
Size: 4316
BamHI G'GATC_C
Cuts at: 294 395 1341 294
Size: 101 946 3269
Fragments arranged by size:
3269 946 101
BanI G'GyrC_C
Cuts at: 947 1247 1283 1332 1746 1994 2029 947
Size: 300 36 49 414 248 35 3234
Fragments arranged by size:
3234 414 300 248 49 36 35
BanII G_rGCy'C
Cuts at: 137 217 809 982 1097 1272 1330 2360 137
Size: 80 592 173 115 175 58 1030 2093
Fragments arranged by size:
2093 1030 592 175 173 115 80 58
BbeI G_GCGC'C
Cuts at: 951 1251 1750 1998 951
Size: 300 499 248 3269
Fragments arranged by size:
3269 499 300 248
BbsI GAAGACnn'nnnn_
Cuts at: 769 769
Size: 4316
BbvCI CC'TCA_GC
Cuts at: 737 737
Size: 4316
BciVI GTATCCnnnnn_n'
Cuts at: 2214 2975 4316 2214
Size: 761 1341 2214
Fragments arranged by size:
2214 1341 761
BclI T'GATC_A
Cuts at: 1836 1836
Size: 4316
BglII A'GATC_T
Cuts at: 1831 3868 1831
Size: 2037 2279
BlpI GC'TnA_GC
Cuts at: 131 503 587 1132 1428 131
Size: 372 84 545 296 3019
Fragments arranged by size:
3019 545 372 296 84
Bme1580I G_kGCm'C
Cuts at: 982 1097 1941 2034 3090 982
Size: 115 844 93 1056 2208
Fragments arranged by size:
2208 1056 844 115 93
BmgBI CAC'GTC
Cuts at: 1206 1206
Size: 4316
BmrI ACTGGGnnnn_n'
Cuts at: 211 810 1528 1609 1944 4088 211
Size: 599 718 81 335 2144 439
Fragments arranged by size:
2144 718 599 439 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 853 883 937 1093 254
Size: 599 30 54 156 3477
Fragments arranged by size:
3477 599 156 54 30
Bpu10I CC'TnA_GC
Cuts at: 494 737 878 494
Size: 243 141 3932
Fragments arranged by size:
3932 243 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1492 2371 2863 3161 793
Size: 699 879 492 298 1948
Fragments arranged by size:
1948 879 699 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 154 4020 154
Size: 3866 450
BsaAI yAC'GTr
Cuts at: 629 1308 1669 629
Size: 679 361 3276
Fragments arranged by size:
3276 679 361
BsaBI GATnn'nnATC
Cuts at: 6 1830 3867 6
Size: 1824 2037 455
Fragments arranged by size:
2037 1824 455
BsaHI Gr'CG_yC
Cuts at: 596 948 1248 1747 1995 2697 4193 596
Size: 352 300 499 248 702 1496 719
Fragments arranged by size:
1496 719 702 499 352 300 248
BsaWI w'CCGG_w
Cuts at: 770 1505 2026 2704 2978 3125 3536 770
Size: 735 521 678 274 147 411 1550
Fragments arranged by size:
1550 735 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 251 295 325 355 850 981 1090 251
Size: 44 30 30 495 131 109 3477
Fragments arranged by size:
3477 495 131 109 44 30 30
BseYI C'CCAG_C
Cuts at: 1227 1741 2499 3076 3572 1227
Size: 514 758 577 496 1971
Fragments arranged by size:
1971 758 577 514 496
BsiEI CG_ry'CG
Cuts at: 104 1858 1904 3112 104
Size: 1754 46 1208 1308
Fragments arranged by size:
1754 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 137 323 707 809 842 1184 1330 2108 2298
Size: 186 384 102 33 342 146 778 190
Cuts at: 2298 3090 137
Size: 792 1363
Fragments arranged by size:
1363 792 778 384 342 190 186 146
102 33
Bsp1286I G_dGCh'C
Cuts at: 137 217 323 707 809 842 982 1097 1184
Size: 80 106 384 102 33 140 115 87
Cuts at: 1184 1272 1330 1941 2034 2108 2298 2360 3090
Size: 88 58 611 93 74 190 62 730
Cuts at: 3090 137
Size: 1363
Fragments arranged by size:
1363 730 611 384 190 140 115 106
102 93 88 87 80 74 62 58
33
BspEI T'CCGG_A
Cuts at: 770 3536 770
Size: 2766 1550
BspHI T'CATG_A
Cuts at: 3492 3492
Size: 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 664 861 1166 1366 1580 1882 2263 664
Size: 197 305 200 214 302 381 2717
Fragments arranged by size:
2717 381 305 302 214 200 197
BsrBI CCG'CTC
Cuts at: 50 540 684 699 2608 2662 4146 4311 50
Size: 490 144 15 1909 54 1484 165 55
Fragments arranged by size:
1909 1484 490 165 144 55 54 15
BsrDI GCAATG_nn'
Cuts at: 2228 2228
Size: 4316
BsrFI r'CCGG_y
Cuts at: 2314 2495 2704 2314
Size: 181 209 3926
Fragments arranged by size:
3926 209 181
BssSI C'ACGA_G
Cuts at: 2587 2945 2587
Size: 358 3958
BstAPI GCAn_nnn'nTGC
Cuts at: 639 1693 639
Size: 1054 3262
BstBI TT'CG_AA
Cuts at: 110 2677 110
Size: 2567 1749
BstYI r'GATC_y
Cuts at: 1 294 395 827 1341 1808 1831 2166 2412
Size: 293 101 432 514 467 23 335 246
Cuts at: 2412 3424 3510 3522 3868 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 514 467 449 432 346 335 293
246 101 86 23 12
BtgI C'CryG_G
Cuts at: 125 899 951 998 1175 1272 125
Size: 774 52 47 177 97 3169
Fragments arranged by size:
3169 774 177 97 52 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2266 2447 2610 2266
Size: 181 163 3972
Fragments arranged by size:
3972 181 163
BtsI GCAGTG_nn'
Cuts at: 261 1559 261
Size: 1298 3018
DraI TTT'AAA
Cuts at: 1315 1315
Size: 4316
DrdI GACnn_nn'nnGTC
Cuts at: 2022 2880 2022
Size: 858 3458
EaeI y'GGCC_r
Cuts at: 1218 1901 2075 2466 2493 3838 1218
Size: 683 174 391 27 1345 1696
Fragments arranged by size:
1696 1345 683 391 174 27
EagI C'GGCC_G
Cuts at: 1901 1901
Size: 4316
EarI CTCTTCn'nnn_
Cuts at: 221 674 2339 2549 221
Size: 453 1665 210 1988
Fragments arranged by size:
1988 1665 453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 688 1280 2524 2834 2980 4126 688
Size: 592 1244 310 146 1146 878
Fragments arranged by size:
1244 1146 878 592 310 146
EcoICRI GAG'CTC
Cuts at: 135 807 1328 135
Size: 672 521 3123
Fragments arranged by size:
3123 672 521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 254 853 883 937 1093 1136 2141 2573
Size: 48 599 30 54 156 43 1005 432
Cuts at: 2573 3320 3588 206
Size: 747 268 934
Fragments arranged by size:
1005 934 747 599 432 268 156 54
48 43 30
EcoNI CCTnn'n_nnAGG
Cuts at: 236 328 390 236
Size: 92 62 4162
Fragments arranged by size:
4162 92 62
EcoO109I rG'GnC_Cy
Cuts at: 979 1015 1093 1455 979
Size: 36 78 362 3840
Fragments arranged by size:
3840 362 78 36
EcoRI G'AATT_C
Cuts at: 1320 1320
Size: 4316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 703 2335 703
Size: 1632 2684
FspI TGC'GCA
Cuts at: 1141 2097 1141
Size: 956 3360
HaeII r_GCGC'y
Cuts at: 529 951 990 1251 1750 1998 3020 3999 529
Size: 422 39 261 499 248 1022 979 846
Fragments arranged by size:
1022 979 846 499 422 261 248 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 372 404 2158 2188 2190 2220 372
Size: 32 1754 30 2 30 2468
Fragments arranged by size:
2468 1754 32 30 30 2
HincII GTy'rAC
Cuts at: 1355 1355
Size: 4316
KasI G'GCGC_C
Cuts at: 947 1247 1746 1994 947
Size: 300 499 248 3269
Fragments arranged by size:
3269 499 300 248
KpnI G_GTAC'C
Cuts at: 1336 1336
Size: 4316
MluI A'CGCG_T
Cuts at: 3749 3749
Size: 4316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 296 2987 3171 296
Size: 2691 184 1441
Fragments arranged by size:
2691 1441 184
MscI TGG'CCA
Cuts at: 2077 2077
Size: 4316
MslI CAynn'nnrTG
Cuts at: 2305 2432 2305
Size: 127 4189
MspA1I CmG'CkG
Cuts at: 366 953 1043 1427 1741 2101 3114 3359 366
Size: 587 90 384 314 360 1013 245 1323
Fragments arranged by size:
1323 1013 587 384 360 314 245 90
NaeI GCC'GGC
Cuts at: 2497 2497
Size: 4316
NarI GG'CG_CC
Cuts at: 948 1248 1747 1995 948
Size: 300 499 248 3269
Fragments arranged by size:
3269 499 300 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4316
NgoMIV G'CCGG_C
Cuts at: 2495 2495
Size: 4316
NspI r_CATG'y
Cuts at: 555 1369 1869 2776 555
Size: 814 500 907 2095
Fragments arranged by size:
2095 907 814 500
PasI CC'CwG_GG
Cuts at: 975 975
Size: 4316
PciI A'CATG_T
Cuts at: 2772 2772
Size: 4316
PflMI CCAn_nnn'nTGG
Cuts at: 831 975 1227 831
Size: 144 252 3920
Fragments arranged by size:
3920 252 144
PfoI T'CCnGG_A
Cuts at: 147 830 147
Size: 683 3633
PmeI GTTT'AAAC
Cuts at: 1315 1315
Size: 4316
PmlI CAC'GTG
Cuts at: 629 629
Size: 4316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 4284
PpuMI rG'GwC_Cy
Cuts at: 1015 1015
Size: 4316
PspOMI G'GGCC_C
Cuts at: 978 1093 978
Size: 115 4201
PstI C_TGCA'G
Cuts at: 761 1163 1363 2048 761
Size: 402 200 685 3029
Fragments arranged by size:
3029 685 402 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4316
PvuII CAG'CTG
Cuts at: 366 1741 2101 366
Size: 1375 360 2581
Fragments arranged by size:
2581 1375 360
RsrII CG'GwC_CG
Cuts at: 2511 2511
Size: 4316
SacI G_AGCT'C
Cuts at: 137 809 1330 137
Size: 672 521 3123
Fragments arranged by size:
3123 672 521
SacII CC_GC'GG
Cuts at: 954 954
Size: 4316
SalI G'TCGA_C
Cuts at: 1353 1353
Size: 4316
SapI GCTCTTCn'nnn_
Cuts at: 221 674 2339 2549 221
Size: 453 1665 210 1988
Fragments arranged by size:
1988 1665 453 210
SbfI CC_TGCA'GG
Cuts at: 1363 1363
Size: 4316
ScaI AGT'ACT
Cuts at: 3833 3833
Size: 4316
SexAI A'CCwGG_T
Cuts at: 350 350
Size: 4316
SfcI C'TryA_G
Cuts at: 33 361 400 757 1159 1359 2044 3037 3228
Size: 328 39 357 402 200 685 993 191
Cuts at: 3228 33
Size: 1121
Fragments arranged by size:
1121 993 685 402 357 328 200 191
39
SfoI GGC'GCC
Cuts at: 949 1249 1748 1996 949
Size: 300 499 248 3269
Fragments arranged by size:
3269 499 300 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
SmaI CCC'GGG
Cuts at: 1338 1338
Size: 4316
SmlI C'TyrA_G
Cuts at: 566 808 1471 2386 2878 3140 566
Size: 242 663 915 492 262 1742
Fragments arranged by size:
1742 915 663 492 262 242
SnaBI TAC'GTA
Cuts at: 1308 1308
Size: 4316
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4316
SphI G_CATG'C
Cuts at: 555 1369 1869 555
Size: 814 500 3002
Fragments arranged by size:
3002 814 500
StuI AGG'CCT
Cuts at: 1193 1193
Size: 4316
StyI C'CwwG_G
Cuts at: 125 208 848 1450 1804 125
Size: 83 640 602 354 2637
Fragments arranged by size:
2637 640 602 354 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1899 2032 2491 2700 1899
Size: 133 459 209 3515
Fragments arranged by size:
3515 459 209 133
TatI w'GTAC_w
Cuts at: 3831 3831
Size: 4316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 613 645 3833 3865 613
Size: 32 3188 32 1064
Fragments arranged by size:
3188 1064 32 32
Tth111I GACn'n_nGTC
Cuts at: 1204 2113 1204
Size: 909 3407
XbaI T'CTAG_A
Cuts at: 1347 1347
Size: 4316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1811 1811
Size: 4316
XhoI C'TCGA_G
Cuts at: 566 566
Size: 4316
XmaI C'CCGG_G
Cuts at: 1336 1336
Size: 4316
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI BaeI
BcgI BfrBI BglI BmtI BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SrfI SspI SwaI
XmnI ZraI