Restriction map for
pF1KSDA1721
(Circular) (Six-base) MAPSORT of: pF1KSDA1721.seq Check: 8659 from: 1 to: 6590
pF1KSDA1721 6590 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1319 1602 3628 1319
Size: 283 2026 4281
Fragments arranged by size:
4281 2026 283
Acc65I G'GTAC_C
Cuts at: 3606 3606
Size: 6590
AclI AA'CG_TT
Cuts at: 6430 6430
Size: 6590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 770 1163 1868 2437 2446 4415 4847 5594 5862
Size: 393 705 569 9 1969 432 747 268
Cuts at: 5862 770
Size: 1498
Fragments arranged by size:
1969 1498 747 705 569 432 393 268
9
AflII C'TTAA_G
Cuts at: 2475 3286 2475
Size: 811 5779
AflIII A'CryG_T
Cuts at: 3362 3942 5046 6023 3362
Size: 580 1104 977 3929
Fragments arranged by size:
3929 1104 977 580
AgeI A'CCGG_T
Cuts at: 4978 4978
Size: 6590
AleI CACnn'nnGTG
Cuts at: 2254 3367 2254
Size: 1113 5477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1037 2237 1037
Size: 1200 5390
AlwNI CAG_nnn'CTG
Cuts at: 1933 2677 3895 5462 1933
Size: 744 1218 1567 3061
Fragments arranged by size:
3061 1567 1218 744
ApaI G_GGCC'C
Cuts at: 149 149
Size: 6590
ApaLI G'TGCA_C
Cuts at: 5360 5360
Size: 6590
ApoI r'AATT_y
Cuts at: 622 667 849 1470 1874 2307 2899 3541 3594
Size: 45 182 621 404 433 592 642 53
Cuts at: 3594 5943 6199 622
Size: 2349 256 1013
Fragments arranged by size:
2349 1013 642 621 592 433 404 256
182 53 45
AseI AT'TA_AT
Cuts at: 20 1859 3073 20
Size: 1839 1214 3537
Fragments arranged by size:
3537 1839 1214
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6590
AvaI C'yCGr_G
Cuts at: 1982 3610 1982
Size: 1628 4962
BamHI G'GATC_C
Cuts at: 3615 3615
Size: 6590
BanI G'GyrC_C
Cuts at: 195 3606 4020 4268 4303 195
Size: 3411 414 248 35 2482
Fragments arranged by size:
3411 2482 414 248 35
BanII G_rGCy'C
Cuts at: 149 3604 4634 149
Size: 3455 1030 2105
Fragments arranged by size:
3455 2105 1030
BbeI G_GCGC'C
Cuts at: 4024 4272 4024
Size: 248 6342
BbsI GAAGACnn'nnnn_
Cuts at: 710 776 710
Size: 66 6524
BbvCI CC'TCA_GC
Cuts at: 2573 2573
Size: 6590
BciVI GTATCCnnnnn_n'
Cuts at: 4488 5249 6590 4488
Size: 761 1341 4488
Fragments arranged by size:
4488 1341 761
BclI T'GATC_A
Cuts at: 1551 2097 4110 1551
Size: 546 2013 4031
Fragments arranged by size:
4031 2013 546
BglII A'GATC_T
Cuts at: 716 1998 2965 4105 6142 716
Size: 1282 967 1140 2037 1164
Fragments arranged by size:
2037 1282 1164 1140 967
BlpI GC'TnA_GC
Cuts at: 164 2315 3702 164
Size: 2151 1387 3052
Fragments arranged by size:
3052 2151 1387
Bme1580I G_kGCm'C
Cuts at: 149 4215 4308 5364 149
Size: 4066 93 1056 1375
Fragments arranged by size:
4066 1375 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1020 3427 3802 3883 4218 6362 1020
Size: 2407 375 81 335 2144 1248
Fragments arranged by size:
2407 2144 1248 375 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2017 4957 2017
Size: 2940 3650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1143 1439 1796 1871 2223 3223 190
Size: 953 296 357 75 352 1000 3557
Fragments arranged by size:
3557 1000 953 357 352 296 75
Bpu10I CC'TnA_GC
Cuts at: 2573 2573
Size: 6590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 846 3766 4645 5137 5435 846
Size: 2920 879 492 298 2001
Fragments arranged by size:
2920 2001 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1532 3568 6294 1532
Size: 2036 2726 1828
Fragments arranged by size:
2726 2036 1828
BsaAI yAC'GTr
Cuts at: 3582 3943 3582
Size: 361 6229
BsaBI GATnn'nnATC
Cuts at: 6 4104 6141 6
Size: 4098 2037 455
Fragments arranged by size:
4098 2037 455
BsaHI Gr'CG_yC
Cuts at: 4021 4269 4971 6467 4021
Size: 248 702 1496 4144
Fragments arranged by size:
4144 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3779 4300 4978 5252 5399 5810 3779
Size: 521 678 274 147 411 4559
Fragments arranged by size:
4559 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1339 1369 1339
Size: 30 6560
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1221 1516 3479 1221
Size: 295 1963 4332
Fragments arranged by size:
4332 1963 295
BseYI C'CCAG_C
Cuts at: 141 2057 4015 4773 5350 5846 141
Size: 1916 1958 758 577 496 885
Fragments arranged by size:
1958 1916 885 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2257 2386 2257
Size: 129 6461
BsiEI CG_ry'CG
Cuts at: 104 4132 4178 5386 104
Size: 4028 46 1208 1308
Fragments arranged by size:
4028 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 242 3604 4382 4572 5364 242
Size: 3362 778 190 792 1468
Fragments arranged by size:
3362 1468 792 778 190
BsmI GAATG_Cn'
Cuts at: 1577 1839 1577
Size: 262 6328
Bsp1286I G_dGCh'C
Cuts at: 149 242 3604 4215 4308 4382 4572 4634 5364
Size: 93 3362 611 93 74 190 62 730
Cuts at: 5364 149
Size: 1375
Fragments arranged by size:
3362 1375 730 611 190 93 93 74
62
BspEI T'CCGG_A
Cuts at: 5810 5810
Size: 6590
BspHI T'CATG_A
Cuts at: 540 5766 540
Size: 5226 1364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2864 2894 3640 3854 4156 4537 2864
Size: 30 746 214 302 381 4917
Fragments arranged by size:
4917 746 381 302 214 30
BsrBI CCG'CTC
Cuts at: 50 4882 4936 6420 6585 50
Size: 4832 54 1484 165 55
Fragments arranged by size:
4832 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2567 4502 2567
Size: 1935 4655
BsrFI r'CCGG_y
Cuts at: 4588 4769 4978 4588
Size: 181 209 6200
Fragments arranged by size:
6200 209 181
BssSI C'ACGA_G
Cuts at: 1496 4861 5219 1496
Size: 3365 358 2867
Fragments arranged by size:
3365 2867 358
BstAPI GCAn_nnn'nTGC
Cuts at: 894 3967 894
Size: 3073 3517
BstBI TT'CG_AA
Cuts at: 110 4951 110
Size: 4841 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1488 1488
Size: 6590
BstYI r'GATC_y
Cuts at: 1 716 1154 1882 1998 2965 3030 3126 3615
Size: 715 438 728 116 967 65 96 489
Cuts at: 3615 4082 4105 4440 4686 5698 5784 5796 6142
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6142 1
Size: 449
Fragments arranged by size:
1012 967 728 715 489 467 449 438
346 335 246 116 96 86 65 23
12
BstZ17I GTA'TAC
Cuts at: 1320 1603 1320
Size: 283 6307
Bsu36I CC'TnA_GG
Cuts at: 3122 3122
Size: 6590
BtgI C'CryG_G
Cuts at: 206 672 1647 206
Size: 466 975 5149
Fragments arranged by size:
5149 975 466
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4540 4721 4884 4540
Size: 181 163 6246
Fragments arranged by size:
6246 181 163
BtsI GCAGTG_nn'
Cuts at: 1021 2540 3338 3473 3833 1021
Size: 1519 798 135 360 3778
Fragments arranged by size:
3778 1519 798 360 135
DraI TTT'AAA
Cuts at: 2051 3135 3589 2051
Size: 1084 454 5052
Fragments arranged by size:
5052 1084 454
DrdI GACnn_nn'nnGTC
Cuts at: 1649 4296 5154 1649
Size: 2647 858 3085
Fragments arranged by size:
3085 2647 858
EaeI y'GGCC_r
Cuts at: 1559 4175 4349 4740 4767 6112 1559
Size: 2616 174 391 27 1345 2037
Fragments arranged by size:
2616 2037 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4175 4175
Size: 6590
EarI CTCTTCn'nnn_
Cuts at: 935 1043 1613 2259 2834 4613 4823 935
Size: 108 570 646 575 1779 210 2702
Fragments arranged by size:
2702 1779 646 575 570 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4798 5108 5254 6400 4798
Size: 310 146 1146 4988
Fragments arranged by size:
4988 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3602 3602
Size: 6590
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 770 1143 1163 1439 1796 1868 1871 2223
Size: 580 373 20 276 357 72 3 352
Cuts at: 2223 2437 2446 3223 4415 4847 5594 5862 190
Size: 214 9 777 1192 432 747 268 918
Fragments arranged by size:
1192 918 777 747 580 432 373 357
352 276 268 214 72 20 9 3
EcoO109I rG'GnC_Cy
Cuts at: 145 146 2217 2556 3729 145
Size: 1 2071 339 1173 3006
Fragments arranged by size:
3006 2071 1173 339 1
EcoRI G'AATT_C
Cuts at: 667 2899 3594 667
Size: 2232 695 3663
Fragments arranged by size:
3663 2232 695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 724 1315 2788 2875 3537 4609 724
Size: 591 1473 87 662 1072 2705
Fragments arranged by size:
2705 1473 1072 662 591 87
FspI TGC'GCA
Cuts at: 4371 4371
Size: 6590
HaeII r_GCGC'y
Cuts at: 142 4024 4272 5294 6273 142
Size: 3882 248 1022 979 459
Fragments arranged by size:
3882 1022 979 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1051 1083 2370 2402 2667 2699 2715 2747 4432
Size: 32 1287 32 265 32 16 32 1685
Cuts at: 4432 4462 4464 4494 1051
Size: 30 2 30 3147
Fragments arranged by size:
3147 1685 1287 265 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 728 1242 1358 1707 1854 3629 728
Size: 514 116 349 147 1775 3689
Fragments arranged by size:
3689 1775 514 349 147 116
HindIII A'AGCT_T
Cuts at: 3353 3353
Size: 6590
HpaI GTT'AAC
Cuts at: 728 1242 1358 728
Size: 514 116 5960
Fragments arranged by size:
5960 514 116
KasI G'GCGC_C
Cuts at: 4020 4268 4020
Size: 248 6342
KpnI G_GTAC'C
Cuts at: 3610 3610
Size: 6590
MfeI C'AATT_G
Cuts at: 518 2634 518
Size: 2116 4474
MluI A'CGCG_T
Cuts at: 6023 6023
Size: 6590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1325 3070 5261 5445 1325
Size: 1745 2191 184 2470
Fragments arranged by size:
2470 2191 1745 184
MscI TGG'CCA
Cuts at: 1561 4351 1561
Size: 2790 3800
MslI CAynn'nnrTG
Cuts at: 2254 3331 3367 4579 4706 2254
Size: 1077 36 1212 127 4138
Fragments arranged by size:
4138 1212 1077 127 36
MspA1I CmG'CkG
Cuts at: 168 2980 3701 4015 4375 5388 5633 168
Size: 2812 721 314 360 1013 245 1125
Fragments arranged by size:
2812 1125 1013 721 360 314 245
NaeI GCC'GGC
Cuts at: 4771 4771
Size: 6590
NarI GG'CG_CC
Cuts at: 4021 4269 4021
Size: 248 6342
NcoI C'CATG_G
Cuts at: 206 672 1647 206
Size: 466 975 5149
Fragments arranged by size:
5149 975 466
NdeI CA'TA_TG
Cuts at: 1332 2411 1332
Size: 1079 5511
NgoMIV G'CCGG_C
Cuts at: 4769 4769
Size: 6590
NspI r_CATG'y
Cuts at: 235 1411 3643 4143 5050 235
Size: 1176 2232 500 907 1775
Fragments arranged by size:
2232 1775 1176 907 500
PasI CC'CwG_GG
Cuts at: 1912 1912
Size: 6590
PciI A'CATG_T
Cuts at: 5046 5046
Size: 6590
PfoI T'CCnGG_A
Cuts at: 1887 1887
Size: 6590
PmeI GTTT'AAAC
Cuts at: 3589 3589
Size: 6590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5729 5761 5729
Size: 32 6558
PpuMI rG'GwC_Cy
Cuts at: 2217 2217
Size: 6590
PshAI GACnn'nnGTC
Cuts at: 710 710
Size: 6590
PspOMI G'GGCC_C
Cuts at: 145 145
Size: 6590
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 455 487 1586 1618 455
Size: 32 1099 32 5427
Fragments arranged by size:
5427 1099 32 32
PstI C_TGCA'G
Cuts at: 746 1016 1593 3637 4322 746
Size: 270 577 2044 685 3014
Fragments arranged by size:
3014 2044 685 577 270
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6590
PvuII CAG'CTG
Cuts at: 168 4015 4375 168
Size: 3847 360 2383
Fragments arranged by size:
3847 2383 360
RsrII CG'GwC_CG
Cuts at: 4785 4785
Size: 6590
SacI G_AGCT'C
Cuts at: 3604 3604
Size: 6590
SalI G'TCGA_C
Cuts at: 3627 3627
Size: 6590
SapI GCTCTTCn'nnn_
Cuts at: 2834 4613 4823 2834
Size: 1779 210 4601
Fragments arranged by size:
4601 1779 210
SbfI CC_TGCA'GG
Cuts at: 3637 3637
Size: 6590
ScaI AGT'ACT
Cuts at: 3064 6107 3064
Size: 3043 3547
SfcI C'TryA_G
Cuts at: 33 742 1012 1589 3633 4318 5311 5502 33
Size: 709 270 577 2044 685 993 191 1121
Fragments arranged by size:
2044 1121 993 709 685 577 270 191
SfoI GGC'GCC
Cuts at: 4022 4270 4022
Size: 248 6342
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6590
SmaI CCC'GGG
Cuts at: 3612 3612
Size: 6590
SmlI C'TyrA_G
Cuts at: 825 1982 2475 3286 3745 4660 5152 5414 825
Size: 1157 493 811 459 915 492 262 2001
Fragments arranged by size:
2001 1157 915 811 493 492 459 262
SnaBI TAC'GTA
Cuts at: 3582 3582
Size: 6590
SpeI A'CTAG_T
Cuts at: 67 304 67
Size: 237 6353
SphI G_CATG'C
Cuts at: 235 3643 4143 235
Size: 3408 500 2682
Fragments arranged by size:
3408 2682 500
SspI AAT'ATT
Cuts at: 1828 3391 1828
Size: 1563 5027
StuI AGG'CCT
Cuts at: 3208 3208
Size: 6590
StyI C'CwwG_G
Cuts at: 206 672 1647 3724 4078 206
Size: 466 975 2077 354 2718
Fragments arranged by size:
2718 2077 975 466 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4173 4306 4765 4974 4173
Size: 133 459 209 5789
Fragments arranged by size:
5789 459 209 133
TatI w'GTAC_w
Cuts at: 966 2119 3062 3146 6105 966
Size: 1153 943 84 2959 1451
Fragments arranged by size:
2959 1451 1153 943 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6107 6139 6107
Size: 32 6558
Tth111I GACn'n_nGTC
Cuts at: 319 4387 319
Size: 4068 2522
XbaI T'CTAG_A
Cuts at: 3621 3621
Size: 6590
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 345 914 4085 345
Size: 569 3171 2850
Fragments arranged by size:
3171 2850 569
XhoI C'TCGA_G
Cuts at: 1982 1982
Size: 6590
XmaI C'CCGG_G
Cuts at: 3610 3610
Size: 6590
XmnI GAAnn'nnTTC
Cuts at: 467 707 1739 1929 467
Size: 240 1032 190 5128
Fragments arranged by size:
5128 1032 240 190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI NheI NotI NruI NsiI PacI PflMI PmlI
PsiI PspXI SacII SanDI SexAI SfiI SgrAI SrfI
SwaI ZraI