Restriction map for
pF1KSDA1723
(Circular) (Six-base) MAPSORT of: pF1KSDA1723.seq Check: 5538 from: 1 to: 7721
pF1KSDA1723 7721 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 880 880
Size: 7721
AccI GT'mk_AC
Cuts at: 4307 4759 4307
Size: 452 7269
Acc65I G'GTAC_C
Cuts at: 4737 4737
Size: 7721
AclI AA'CG_TT
Cuts at: 7561 7561
Size: 7721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2055 2061 2167 2269 2576 2734 3447 3595 3862
Size: 6 106 102 307 158 713 148 267
Cuts at: 3862 4051 4653 4664 5546 5978 6725 6993 2055
Size: 189 602 11 882 432 747 268 2783
Fragments arranged by size:
2783 882 747 713 602 432 307 268
267 189 158 148 106 102 11 6
AflII C'TTAA_G
Cuts at: 720 4587 720
Size: 3867 3854
AflIII A'CryG_T
Cuts at: 162 4571 5073 6177 7154 162
Size: 4409 502 1104 977 729
Fragments arranged by size:
4409 1104 977 729 502
AgeI A'CCGG_T
Cuts at: 6109 6109
Size: 7721
AhdI GACnn_n'nnGTC
Cuts at: 1377 2120 1377
Size: 743 6978
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2527 2559 2527
Size: 32 7689
AlwNI CAG_nnn'CTG
Cuts at: 1315 3498 3672 3735 5026 6593 1315
Size: 2183 174 63 1291 1567 2443
Fragments arranged by size:
2443 2183 1567 1291 174 63
ApaI G_GGCC'C
Cuts at: 2216 2216
Size: 7721
ApaLI G'TGCA_C
Cuts at: 6491 6491
Size: 7721
ApoI r'AATT_y
Cuts at: 1359 3852 4334 4355 4725 7074 7330 1359
Size: 2493 482 21 370 2349 256 1750
Fragments arranged by size:
2493 2349 1750 482 370 256 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 7721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7721
AvaI C'yCGr_G
Cuts at: 2613 2836 3985 4054 4397 4741 2613
Size: 223 1149 69 343 344 5593
Fragments arranged by size:
5593 1149 344 343 223 69
BamHI G'GATC_C
Cuts at: 4746 4746
Size: 7721
BanI G'GyrC_C
Cuts at: 3116 3517 4058 4383 4737 5151 5399 5434 3116
Size: 401 541 325 354 414 248 35 5403
Fragments arranged by size:
5403 541 414 401 354 325 248 35
BanII G_rGCy'C
Cuts at: 2161 2216 2779 3056 3148 3461 4735 5765 2161
Size: 55 563 277 92 313 1274 1030 4117
Fragments arranged by size:
4117 1274 1030 563 313 277 92 55
BbeI G_GCGC'C
Cuts at: 5155 5403 5155
Size: 248 7473
BbsI GAAGACnn'nnnn_
Cuts at: 1358 1892 1358
Size: 534 7187
BbvCI CC'TCA_GC
Cuts at: 1243 1879 1915 1243
Size: 636 36 7049
Fragments arranged by size:
7049 636 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1945 1979 2083 2117 3568 3602 1945
Size: 34 104 34 1451 34 6064
Fragments arranged by size:
6064 1451 104 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 753 5619 6380 7721 753
Size: 4866 761 1341 753
Fragments arranged by size:
4866 1341 761 753
BclI T'GATC_A
Cuts at: 1221 2201 5241 1221
Size: 980 3040 3701
Fragments arranged by size:
3701 3040 980
BglI GCCn_nnn'nGGC
Cuts at: 1615 1862 3685 1615
Size: 247 1823 5651
Fragments arranged by size:
5651 1823 247
BglII A'GATC_T
Cuts at: 2270 3591 3641 5236 7273 2270
Size: 1321 50 1595 2037 2718
Fragments arranged by size:
2718 2037 1595 1321 50
BlpI GC'TnA_GC
Cuts at: 821 3810 4181 4833 821
Size: 2989 371 652 3709
Fragments arranged by size:
3709 2989 652 371
Bme1580I G_kGCm'C
Cuts at: 2216 3302 3659 4061 4455 5346 5439 6495 2216
Size: 1086 357 402 394 891 93 1056 3442
Fragments arranged by size:
3442 1086 1056 891 402 394 357 93
BmgBI CAC'GTC
Cuts at: 3561 3561
Size: 7721
BmrI ACTGGGnnnn_n'
Cuts at: 1518 4933 5014 5349 7493 1518
Size: 3415 81 335 2144 1746
Fragments arranged by size:
3415 2144 1746 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3873 6088 3873
Size: 2215 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1027 1388 1989 2658 2775 2836 3274 4084
Size: 309 361 601 669 117 61 438 810
Cuts at: 4084 4443 718
Size: 359 3996
Fragments arranged by size:
3996 810 669 601 438 361 359 309
117 61
Bpu10I CC'TnA_GC
Cuts at: 1243 1879 1915 2752 3599 3748 1243
Size: 636 36 837 847 149 5216
Fragments arranged by size:
5216 847 837 636 149 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2137 3920 4897 5776 6268 6566 2137
Size: 1783 977 879 492 298 3292
Fragments arranged by size:
3292 1783 977 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1087 2362 2949 7425 1087
Size: 1275 587 4476 1383
Fragments arranged by size:
4476 1383 1275 587
BsaAI yAC'GTr
Cuts at: 2862 4713 5074 2862
Size: 1851 361 5509
Fragments arranged by size:
5509 1851 361
BsaBI GATnn'nnATC
Cuts at: 6 4478 5235 7272 6
Size: 4472 757 2037 455
Fragments arranged by size:
4472 2037 757 455
BsaHI Gr'CG_yC
Cuts at: 1629 5152 5400 6102 7598 1629
Size: 3523 248 702 1496 1752
Fragments arranged by size:
3523 1752 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3045 3341 3431 4910 5431 6109 6383 6530 6941
Size: 296 90 1479 521 678 274 147 411
Cuts at: 6941 3045
Size: 3825
Fragments arranged by size:
3825 1479 678 521 411 296 274 147
90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2406 2436 4082 4112 2406
Size: 30 1646 30 6015
Fragments arranged by size:
6015 1646 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1245 1992 2254 2334 1245
Size: 747 262 80 6632
Fragments arranged by size:
6632 747 262 80
BseYI C'CCAG_C
Cuts at: 152 1852 1936 1951 2193 2392 3007 3290 4151
Size: 1700 84 15 242 199 615 283 861
Cuts at: 4151 5146 5904 6481 6977 152
Size: 995 758 577 496 896
Fragments arranged by size:
1700 995 896 861 758 615 577 496
283 242 199 84 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3397 4596 4658 3397
Size: 1199 62 6460
Fragments arranged by size:
6460 1199 62
BsiEI CG_ry'CG
Cuts at: 104 2997 4482 5263 5309 6517 104
Size: 2893 1485 781 46 1208 1308
Fragments arranged by size:
2893 1485 1308 1208 781 46
BsiHKAI G_wGCw'C
Cuts at: 2161 3148 4517 4735 5513 5703 6495 2161
Size: 987 1369 218 778 190 792 3387
Fragments arranged by size:
3387 1369 987 792 778 218 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2712 2712
Size: 7721
Bsp1286I G_dGCh'C
Cuts at: 2161 2216 2779 3056 3148 3302 3461 3659 4061
Size: 55 563 277 92 154 159 198 402
Cuts at: 4061 4455 4517 4735 5346 5439 5513 5703 5765
Size: 394 62 218 611 93 74 190 62
Cuts at: 5765 6495 2161
Size: 730 3387
Fragments arranged by size:
3387 730 611 563 402 394 277 218
198 190 159 154 93 92 74 62
62 55
BspEI T'CCGG_A
Cuts at: 3431 6941 3431
Size: 3510 4211
BspHI T'CATG_A
Cuts at: 3308 6897 3308
Size: 3589 4132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 880 1289 3221 4771 4985 5287 5668 880
Size: 409 1932 1550 214 302 381 2933
Fragments arranged by size:
2933 1932 1550 409 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6013 6067 7551 7716 50
Size: 5963 54 1484 165 55
Fragments arranged by size:
5963 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 714 882 2028 5633 714
Size: 168 1146 3605 2802
Fragments arranged by size:
3605 2802 1146 168
BsrFI r'CCGG_y
Cuts at: 5719 5900 6109 5719
Size: 181 209 7331
Fragments arranged by size:
7331 209 181
BssSI C'ACGA_G
Cuts at: 1352 5992 6350 1352
Size: 4640 358 2723
Fragments arranged by size:
4640 2723 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3420 3735 5098 3420
Size: 315 1363 6043
Fragments arranged by size:
6043 1363 315
BstBI TT'CG_AA
Cuts at: 110 6082 110
Size: 5972 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2013 2662 3015 2013
Size: 649 353 6719
Fragments arranged by size:
6719 649 353
BstYI r'GATC_y
Cuts at: 1 1312 2270 2811 3062 3591 3641 4655 4746
Size: 1311 958 541 251 529 50 1014 91
Cuts at: 4746 5213 5236 5571 5817 6829 6915 6927 7273
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7273 1
Size: 449
Fragments arranged by size:
1311 1014 1012 958 541 529 467 449
346 335 251 246 91 86 50 23
12
Bsu36I CC'TnA_GG
Cuts at: 2677 2677
Size: 7721
BtgI C'CryG_G
Cuts at: 1187 1382 2306 2655 1187
Size: 195 924 349 6253
Fragments arranged by size:
6253 924 349 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3767 5671 5852 6015 3767
Size: 1904 181 163 5473
Fragments arranged by size:
5473 1904 181 163
BtsI GCAGTG_nn'
Cuts at: 574 761 1081 4964 574
Size: 187 320 3883 3331
Fragments arranged by size:
3883 3331 320 187
ClaI AT'CG_AT
Cuts at: 1562 1562
Size: 7721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 359 394 359
Size: 35 7686
DraI TTT'AAA
Cuts at: 509 1643 4125 4146 4720 509
Size: 1134 2482 21 574 3510
Fragments arranged by size:
3510 2482 1134 574 21
DrdI GACnn_nn'nnGTC
Cuts at: 5427 6285 5427
Size: 858 6863
EaeI y'GGCC_r
Cuts at: 1100 2664 3154 3800 5306 5480 5871 5898 7243
Size: 1564 490 646 1506 174 391 27 1345
Cuts at: 7243 1100
Size: 1578
Fragments arranged by size:
1578 1564 1506 1345 646 490 391 174
27
EagI C'GGCC_G
Cuts at: 5306 5306
Size: 7721
EarI CTCTTCn'nnn_
Cuts at: 1006 1772 2710 3044 3307 3466 3829 3838 5744
Size: 766 938 334 263 159 363 9 1906
Cuts at: 5744 5954 1006
Size: 210 2773
Fragments arranged by size:
2773 1906 938 766 363 334 263 210
159 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2265 2824 5929 6239 6385 7531 2265
Size: 559 3105 310 146 1146 2455
Fragments arranged by size:
3105 2455 1146 559 310 146
EcoICRI GAG'CTC
Cuts at: 2159 3146 4733 2159
Size: 987 1587 5147
Fragments arranged by size:
5147 1587 987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1027 1388 1989 2055 2061 2167 2269 2576
Size: 309 361 601 66 6 106 102 307
Cuts at: 2576 2658 2734 2775 2836 3274 3447 3595 3862
Size: 82 76 41 61 438 173 148 267
Cuts at: 3862 4051 4084 4443 4653 4664 5546 5978 6725
Size: 189 33 359 210 11 882 432 747
Cuts at: 6725 6993 718
Size: 268 1446
Fragments arranged by size:
1446 882 747 601 438 432 361 359
309 307 268 267 210 189 173 148
106 102 82 76 66 61 41 33
11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1840 3861 1840
Size: 2021 5700
EcoO109I rG'GnC_Cy
Cuts at: 1820 2408 2802 3357 4210 4860 1820
Size: 588 394 555 853 650 4681
Fragments arranged by size:
4681 853 650 588 555 394
EcoRI G'AATT_C
Cuts at: 3852 4725 3852
Size: 873 6848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1150 5740 1150
Size: 4590 3131
FspI TGC'GCA
Cuts at: 5502 5502
Size: 7721
HaeII r_GCGC'y
Cuts at: 1890 2185 3672 3817 4278 5155 5403 6425 7404
Size: 295 1487 145 461 877 248 1022 979
Cuts at: 7404 1890
Size: 2207
Fragments arranged by size:
2207 1487 1022 979 877 461 295 248
145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1291 1323 1363 1378 1395 1410 2106 2138 2148
Size: 32 40 15 17 15 696 32 10
Cuts at: 2148 2180 2871 2903 2911 2929 2943 2961 3135
Size: 32 691 32 8 18 14 18 174
Cuts at: 3135 3167 3480 3512 3987 4019 4687 4719 5563
Size: 32 313 32 475 32 668 32 844
Cuts at: 5563 5593 5595 5625 1291
Size: 30 2 30 3387
Fragments arranged by size:
3387 844 696 691 668 475 313 174
40 32 32 32 32 32 32 32
32 30 30 18 18 17 15 15
14 10 8 2
HincII GTy'rAC
Cuts at: 2480 3372 3534 4584 4760 2480
Size: 892 162 1050 176 5441
Fragments arranged by size:
5441 1050 892 176 162
HindIII A'AGCT_T
Cuts at: 717 1490 2649 3970 717
Size: 773 1159 1321 4468
Fragments arranged by size:
4468 1321 1159 773
KasI G'GCGC_C
Cuts at: 5151 5399 5151
Size: 248 7473
KpnI G_GTAC'C
Cuts at: 4741 4741
Size: 7721
MfeI C'AATT_G
Cuts at: 796 796
Size: 7721
MluI A'CGCG_T
Cuts at: 7154 7154
Size: 7721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2354 2876 3134 4295 6392 6576 2345
Size: 9 522 258 1161 2097 184 3490
Fragments arranged by size:
3490 2097 1161 522 258 184 9
MscI TGG'CCA
Cuts at: 1102 2666 5482 1102
Size: 1564 2816 3341
Fragments arranged by size:
3341 2816 1564
MslI CAynn'nnrTG
Cuts at: 401 1870 2981 4570 4634 5710 5837 401
Size: 1469 1111 1589 64 1076 127 2285
Fragments arranged by size:
2285 1589 1469 1111 1076 127 64
MspA1I CmG'CkG
Cuts at: 994 2210 2877 4081 4642 4832 5146 5506 6519
Size: 1216 667 1204 561 190 314 360 1013
Cuts at: 6519 6764 994
Size: 245 1951
Fragments arranged by size:
1951 1216 1204 1013 667 561 360 314
245 190
NaeI GCC'GGC
Cuts at: 5902 5902
Size: 7721
NarI GG'CG_CC
Cuts at: 5152 5400 5152
Size: 248 7473
NcoI C'CATG_G
Cuts at: 1187 2306 1187
Size: 1119 6602
NdeI CA'TA_TG
Cuts at: 542 3950 542
Size: 3408 4313
NgoMIV G'CCGG_C
Cuts at: 5900 5900
Size: 7721
NspI r_CATG'y
Cuts at: 166 218 454 1074 2448 3573 4575 4602 4774
Size: 52 236 620 1374 1125 1002 27 172
Cuts at: 4774 5274 6181 166
Size: 500 907 1706
Fragments arranged by size:
1706 1374 1125 1002 907 620 500 236
172 52 27
PasI CC'CwG_GG
Cuts at: 1816 3863 1816
Size: 2047 5674
PciI A'CATG_T
Cuts at: 162 4571 6177 162
Size: 4409 1606 1706
Fragments arranged by size:
4409 1706 1606
PflMI CCAn_nnn'nTGG
Cuts at: 177 3198 3444 177
Size: 3021 246 4454
Fragments arranged by size:
4454 3021 246
PfoI T'CCnGG_A
Cuts at: 3065 3484 4100 4658 3065
Size: 419 616 558 6128
Fragments arranged by size:
6128 616 558 419
PmeI GTTT'AAAC
Cuts at: 4720 4720
Size: 7721
PmlI CAC'GTG
Cuts at: 2862 2862
Size: 7721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3607 3639 4280 4312 6860 6892 3607
Size: 32 641 32 2548 32 4436
Fragments arranged by size:
4436 2548 641 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1820 2408 2802 3357 4210 1820
Size: 588 394 555 853 5331
Fragments arranged by size:
5331 853 588 555 394
PshAI GACnn'nnGTC
Cuts at: 317 317
Size: 7721
PspOMI G'GGCC_C
Cuts at: 2212 2212
Size: 7721
PstI C_TGCA'G
Cuts at: 965 3221 3677 3731 4768 5453 965
Size: 2256 456 54 1037 685 3233
Fragments arranged by size:
3233 2256 1037 685 456 54
PvuI CG_AT'CG
Cuts at: 104 4482 104
Size: 4378 3343
PvuII CAG'CTG
Cuts at: 994 4081 4642 5146 5506 994
Size: 3087 561 504 360 3209
Fragments arranged by size:
3209 3087 561 504 360
RsrII CG'GwC_CG
Cuts at: 5916 5916
Size: 7721
SacI G_AGCT'C
Cuts at: 2161 3148 4735 2161
Size: 987 1587 5147
Fragments arranged by size:
5147 1587 987
SalI G'TCGA_C
Cuts at: 4758 4758
Size: 7721
SanDI GG'GwC_CC
Cuts at: 1820 3357 1820
Size: 1537 6184
SapI GCTCTTCn'nnn_
Cuts at: 3044 3466 5744 5954 3044
Size: 422 2278 210 4811
Fragments arranged by size:
4811 2278 422 210
SbfI CC_TGCA'GG
Cuts at: 3677 4768 3677
Size: 1091 6630
ScaI AGT'ACT
Cuts at: 7238 7238
Size: 7721
SexAI A'CCwGG_T
Cuts at: 1808 3103 1808
Size: 1295 6426
SfcI C'TryA_G
Cuts at: 33 131 961 3178 3217 3637 3673 3727 4764
Size: 98 830 2217 39 420 36 54 1037
Cuts at: 4764 5449 6442 6633 33
Size: 685 993 191 1121
Fragments arranged by size:
2217 1121 1037 993 830 685 420 191
98 54 39 36
SfoI GGC'GCC
Cuts at: 5153 5401 5153
Size: 248 7473
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7721
SmaI CCC'GGG
Cuts at: 4743 4743
Size: 7721
SmlI C'TyrA_G
Cuts at: 720 2152 3935 4397 4587 4876 5791 6283 6545
Size: 1432 1783 462 190 289 915 492 262
Cuts at: 6545 720
Size: 1896
Fragments arranged by size:
1896 1783 1432 915 492 462 289 262
190
SnaBI TAC'GTA
Cuts at: 4713 4713
Size: 7721
SpeI A'CTAG_T
Cuts at: 67 228 444 67
Size: 161 216 7344
Fragments arranged by size:
7344 216 161
SphI G_CATG'C
Cuts at: 1074 4774 5274 1074
Size: 3700 500 3521
Fragments arranged by size:
3700 3521 500
StyI C'CwwG_G
Cuts at: 516 549 1187 1484 1571 2054 2306 2705 3659
Size: 33 638 297 87 483 252 399 954
Cuts at: 3659 4855 5209 516
Size: 1196 354 3028
Fragments arranged by size:
3028 1196 954 638 483 399 354 297
252 87 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 756 1120 2931 3011 3021 4486 5304 5437 5896
Size: 364 1811 80 10 1465 818 133 459
Cuts at: 5896 6105 756
Size: 209 2372
Fragments arranged by size:
2372 1811 1465 818 459 364 209 133
80 10
TatI w'GTAC_w
Cuts at: 882 2447 7236 882
Size: 1565 4789 1367
Fragments arranged by size:
4789 1565 1367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1677 1709 2406 2438 7238 7270 1677
Size: 32 697 32 4800 32 2128
Fragments arranged by size:
4800 2128 697 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1810 2941 3529 5518 1810
Size: 1131 588 1989 4013
Fragments arranged by size:
4013 1989 1131 588
XbaI T'CTAG_A
Cuts at: 4752 4752
Size: 7721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3939 5216 3939
Size: 1277 6444
XhoI C'TCGA_G
Cuts at: 4397 4397
Size: 7721
XmaI C'CCGG_G
Cuts at: 4741 4741
Size: 7721
XmnI GAAnn'nnTTC
Cuts at: 3078 3632 3973 3078
Size: 554 341 6826
Fragments arranged by size:
6826 554 341
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI AlfI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I DraIII
EcoRV FseI FspAI HpaI NheI NotI NruI NsiI
PacI PsiI PspXI PsrI SacII SfiI SgrAI SrfI
SspI StuI SwaI ZraI