Restriction map for
pF1KSDA1724
(Circular) (Six-base) MAPSORT of: pF1KSDA1724.seq Check: 9435 from: 1 to: 4295
pF1KSDA1724 4295 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 713 943 1168 1333 713
Size: 230 225 165 3675
Fragments arranged by size:
3675 230 225 165
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 2120 2552 3299 3567 953
Size: 1167 432 747 268 1681
Fragments arranged by size:
1681 1167 747 432 268
AflIII A'CryG_T
Cuts at: 1036 1647 2751 3728 1036
Size: 611 1104 977 1603
Fragments arranged by size:
1603 1104 977 611
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AlwNI CAG_nnn'CTG
Cuts at: 1600 3167 1600
Size: 1567 2728
ApaLI G'TGCA_C
Cuts at: 3065 3065
Size: 4295
ApoI r'AATT_y
Cuts at: 1299 3648 3904 1299
Size: 2349 256 1690
Fragments arranged by size:
2349 1690 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 1315 1315
Size: 4295
AvrII C'CTAG_G
Cuts at: 1131 1131
Size: 4295
BamHI G'GATC_C
Cuts at: 953 1320 953
Size: 367 3928
BanI G'GyrC_C
Cuts at: 270 1311 1725 1973 2008 270
Size: 1041 414 248 35 2557
Fragments arranged by size:
2557 1041 414 248 35
BanII G_rGCy'C
Cuts at: 151 1309 2339 151
Size: 1158 1030 2107
Fragments arranged by size:
2107 1158 1030
BbeI G_GCGC'C
Cuts at: 274 1729 1977 274
Size: 1455 248 2592
Fragments arranged by size:
2592 1455 248
BbvCI CC'TCA_GC
Cuts at: 357 357
Size: 4295
BciVI GTATCCnnnnn_n'
Cuts at: 189 2193 2954 4295 189
Size: 2004 761 1341 189
Fragments arranged by size:
2004 1341 761 189
BclI T'GATC_A
Cuts at: 498 1815 498
Size: 1317 2978
BfrBI ATG'CAT
Cuts at: 225 225
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 1231 1407 1231
Size: 176 4119
Bme1580I G_kGCm'C
Cuts at: 1920 2013 3069 1920
Size: 93 1056 3146
Fragments arranged by size:
3146 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 396 648 1507 1588 1923 4067 396
Size: 252 859 81 335 2144 624
Fragments arranged by size:
2144 859 624 335 252 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4295
Bpu10I CC'TnA_GC
Cuts at: 357 357
Size: 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1471 2350 2842 3140 1471
Size: 879 492 298 2626
Fragments arranged by size:
2626 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 776 3999 776
Size: 3223 1072
BsaAI yAC'GTr
Cuts at: 144 378 1287 1648 144
Size: 234 909 361 2791
Fragments arranged by size:
2791 909 361 234
BsaBI GATnn'nnATC
Cuts at: 6 200 1809 3846 6
Size: 194 1609 2037 455
Fragments arranged by size:
2037 1609 455 194
BsaHI Gr'CG_yC
Cuts at: 271 1726 1974 2676 4172 271
Size: 1455 248 702 1496 394
Fragments arranged by size:
1496 1455 702 394 248
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 2957 3104 3515 1484
Size: 521 678 274 147 411 2264
Fragments arranged by size:
2264 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1202 1232 1202
Size: 30 4265
BseYI C'CCAG_C
Cuts at: 1720 2478 3055 3551 1720
Size: 758 577 496 2464
Fragments arranged by size:
2464 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3091 104
Size: 1733 46 1208 1308
Fragments arranged by size:
1733 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1309 2087 2277 3069 1309
Size: 778 190 792 2535
Fragments arranged by size:
2535 792 778 190
BsmI GAATG_Cn'
Cuts at: 1157 1157
Size: 4295
Bsp1286I G_dGCh'C
Cuts at: 151 1309 1920 2013 2087 2277 2339 3069 151
Size: 1158 611 93 74 190 62 730 1377
Fragments arranged by size:
1377 1158 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 3471 3471
Size: 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1345 1559 1861 2242 1345
Size: 214 302 381 3398
Fragments arranged by size:
3398 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 803 2207 803
Size: 1404 2891
BsrFI r'CCGG_y
Cuts at: 2293 2474 2683 2293
Size: 181 209 3905
Fragments arranged by size:
3905 209 181
BssSI C'ACGA_G
Cuts at: 2566 2924 2566
Size: 358 3937
BstAPI GCAn_nnn'nTGC
Cuts at: 1672 1672
Size: 4295
BstBI TT'CG_AA
Cuts at: 110 2656 110
Size: 2546 1749
BstEII G'GTnAC_C
Cuts at: 962 962
Size: 4295
BstYI r'GATC_y
Cuts at: 1 953 1320 1787 1810 2145 2391 3403 3489
Size: 952 367 467 23 335 246 1012 86
Cuts at: 3489 3501 3847 1
Size: 12 346 449
Fragments arranged by size:
1012 952 467 449 367 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1169 1169
Size: 4295
Bsu36I CC'TnA_GG
Cuts at: 483 483
Size: 4295
BtgI C'CryG_G
Cuts at: 125 673 125
Size: 548 3747
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 791 1538 791
Size: 747 3548
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 2001 2859 2001
Size: 858 3437
EaeI y'GGCC_r
Cuts at: 1880 2054 2445 2472 3817 1880
Size: 174 391 27 1345 2358
Fragments arranged by size:
2358 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 555 833 1111 2318 2528 555
Size: 278 278 1207 210 2322
Fragments arranged by size:
2322 1207 278 278 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2503 2813 2959 4105 2503
Size: 310 146 1146 2693
Fragments arranged by size:
2693 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 2120 2552 3299 3567 953
Size: 1167 432 747 268 1681
Fragments arranged by size:
1681 1167 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1434 1434
Size: 4295
EcoRI G'AATT_C
Cuts at: 1299 1299
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 274 1729 1977 2999 3978 274
Size: 1455 248 1022 979 591
Fragments arranged by size:
1455 1022 979 591 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 2137 2167 2169 2199 502
Size: 32 1603 30 2 30 2598
Fragments arranged by size:
2598 1603 32 30 30 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
KasI G'GCGC_C
Cuts at: 270 1725 1973 270
Size: 1455 248 2592
Fragments arranged by size:
2592 1455 248
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 993 1102 2966 3150 993
Size: 109 1864 184 2138
Fragments arranged by size:
2138 1864 184 109
MscI TGG'CCA
Cuts at: 2056 2056
Size: 4295
MslI CAynn'nnrTG
Cuts at: 796 2284 2411 796
Size: 1488 127 2680
Fragments arranged by size:
2680 1488 127
MspA1I CmG'CkG
Cuts at: 159 1044 1230 1406 1720 2080 3093 3338 159
Size: 885 186 176 314 360 1013 245 1116
Fragments arranged by size:
1116 1013 885 360 314 245 186 176
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 271 1726 1974 271
Size: 1455 248 2592
Fragments arranged by size:
2592 1455 248
NcoI C'CATG_G
Cuts at: 125 673 125
Size: 548 3747
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NsiI A_TGCA'T
Cuts at: 227 227
Size: 4295
NspI r_CATG'y
Cuts at: 1040 1348 1848 2755 1040
Size: 308 500 907 2580
Fragments arranged by size:
2580 907 500 308
PciI A'CATG_T
Cuts at: 1036 2751 1036
Size: 1715 2580
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PmlI CAC'GTG
Cuts at: 378 378
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PstI C_TGCA'G
Cuts at: 729 1342 2027 729
Size: 613 685 2997
Fragments arranged by size:
2997 685 613
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 159 1044 1230 1720 2080 159
Size: 885 186 490 360 2374
Fragments arranged by size:
2374 885 490 360 186
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SexAI A'CCwGG_T
Cuts at: 363 363
Size: 4295
SfcI C'TryA_G
Cuts at: 33 242 725 944 1338 2023 3016 3207 33
Size: 209 483 219 394 685 993 191 1121
Fragments arranged by size:
1121 993 685 483 394 219 209 191
SfoI GGC'GCC
Cuts at: 272 1727 1975 272
Size: 1455 248 2592
Fragments arranged by size:
2592 1455 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 1450 2365 2857 3119 1450
Size: 915 492 262 2626
Fragments arranged by size:
2626 915 492 262
SnaBI TAC'GTA
Cuts at: 1287 1287
Size: 4295
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StuI AGG'CCT
Cuts at: 745 745
Size: 4295
StyI C'CwwG_G
Cuts at: 125 673 1131 1429 1783 125
Size: 548 458 298 354 2637
Fragments arranged by size:
2637 548 458 354 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1878 2011 2470 2679 1878
Size: 133 459 209 3494
Fragments arranged by size:
3494 459 209 133
TatI w'GTAC_w
Cuts at: 176 182 1033 3810 176
Size: 6 851 2777 661
Fragments arranged by size:
2777 851 661 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1202 1234 3812 3844 1202
Size: 32 2578 32 1653
Fragments arranged by size:
2578 1653 32 32
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 432 1326 432
Size: 894 3401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1085 1204 1790 1085
Size: 119 586 3590
Fragments arranged by size:
3590 586 119
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
XmnI GAAnn'nnTTC
Cuts at: 468 468
Size: 4295
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BcgI BglI BmgBI BmtI
BpmI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstXI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI
ZraI