Restriction map for
pF1KSDA1733
(Circular) (Six-base) MAPSORT of: pF1KSDA1733.seq Check: 9642 from: 1 to: 4589
pF1KSDA1733 4589 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1300 1627 1300
Size: 327 4262
Acc65I G'GTAC_C
Cuts at: 1605 1605
Size: 4589
AclI AA'CG_TT
Cuts at: 4429 4429
Size: 4589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 2414 2846 3593 3861 622
Size: 1792 432 747 268 1350
Fragments arranged by size:
1792 1350 747 432 268
AflIII A'CryG_T
Cuts at: 530 1941 3045 4022 530
Size: 1411 1104 977 1097
Fragments arranged by size:
1411 1104 1097 977
AgeI A'CCGG_T
Cuts at: 2977 2977
Size: 4589
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4589
AlwNI CAG_nnn'CTG
Cuts at: 675 793 1104 1894 3461 675
Size: 118 311 790 1567 1803
Fragments arranged by size:
1803 1567 790 311 118
ApaLI G'TGCA_C
Cuts at: 3359 3359
Size: 4589
ApoI r'AATT_y
Cuts at: 1593 3942 4198 1593
Size: 2349 256 1984
Fragments arranged by size:
2349 1984 256
AseI AT'TA_AT
Cuts at: 20 262 20
Size: 242 4347
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
AvaI C'yCGr_G
Cuts at: 1047 1164 1609 1047
Size: 117 445 4027
Fragments arranged by size:
4027 445 117
BamHI G'GATC_C
Cuts at: 738 1614 738
Size: 876 3713
BanI G'GyrC_C
Cuts at: 427 796 1501 1605 2019 2267 2302 427
Size: 369 705 104 414 248 35 2714
Fragments arranged by size:
2714 705 414 369 248 104 35
BanII G_rGCy'C
Cuts at: 225 682 1073 1603 2633 225
Size: 457 391 530 1030 2181
Fragments arranged by size:
2181 1030 530 457 391
BbeI G_GCGC'C
Cuts at: 800 2023 2271 800
Size: 1223 248 3118
Fragments arranged by size:
3118 1223 248
BbsI GAAGACnn'nnnn_
Cuts at: 1289 1340 1289
Size: 51 4538
BbvCI CC'TCA_GC
Cuts at: 377 1486 377
Size: 1109 3480
BciVI GTATCCnnnnn_n'
Cuts at: 2487 3248 4589 2487
Size: 761 1341 2487
Fragments arranged by size:
2487 1341 761
BclI T'GATC_A
Cuts at: 2109 2109
Size: 4589
BglII A'GATC_T
Cuts at: 2104 4141 2104
Size: 2037 2552
BlpI GC'TnA_GC
Cuts at: 1522 1701 1522
Size: 179 4410
Bme1580I G_kGCm'C
Cuts at: 2214 2307 3363 2214
Size: 93 1056 3440
Fragments arranged by size:
3440 1056 93
BmgBI CAC'GTC
Cuts at: 533 533
Size: 4589
BmrI ACTGGGnnnn_n'
Cuts at: 1801 1882 2217 4361 1801
Size: 81 335 2144 2029
Fragments arranged by size:
2144 2029 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 4589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 666 1501 666
Size: 835 3754
Bpu10I CC'TnA_GC
Cuts at: 377 676 1395 1486 377
Size: 299 719 91 3480
Fragments arranged by size:
3480 719 299 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 1765 2644 3136 3434 209
Size: 1556 879 492 298 1364
Fragments arranged by size:
1556 1364 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4293 4293
Size: 4589
BsaAI yAC'GTr
Cuts at: 1581 1942 1581
Size: 361 4228
BsaBI GATnn'nnATC
Cuts at: 6 2103 4140 6
Size: 2097 2037 455
Fragments arranged by size:
2097 2037 455
BsaHI Gr'CG_yC
Cuts at: 797 2020 2268 2970 4466 797
Size: 1223 248 702 1496 920
Fragments arranged by size:
1496 1223 920 702 248
BsaWI w'CCGG_w
Cuts at: 305 1778 2299 2977 3251 3398 3809 305
Size: 1473 521 678 274 147 411 1085
Fragments arranged by size:
1473 1085 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1000 1030 1345 1375 1405 1435 1000
Size: 30 315 30 30 30 4154
Fragments arranged by size:
4154 315 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 608 1327 1336 1339 1486 608
Size: 719 9 3 147 3711
Fragments arranged by size:
3711 719 147 9 3
BseYI C'CCAG_C
Cuts at: 941 2014 2772 3349 3845 941
Size: 1073 758 577 496 1685
Fragments arranged by size:
1685 1073 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2131 2177 3385 104
Size: 2027 46 1208 1308
Fragments arranged by size:
2027 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 225 682 761 1073 1603 2381 2571 3363 225
Size: 457 79 312 530 778 190 792 1451
Fragments arranged by size:
1451 792 778 530 457 312 190 79
Bsp1286I G_dGCh'C
Cuts at: 225 682 761 1073 1603 2214 2307 2381 2571
Size: 457 79 312 530 611 93 74 190
Cuts at: 2571 2633 3363 225
Size: 62 730 1451
Fragments arranged by size:
1451 730 611 530 457 312 190 93
79 74 62
BspEI T'CCGG_A
Cuts at: 305 3809 305
Size: 3504 1085
BspHI T'CATG_A
Cuts at: 860 1266 3765 860
Size: 406 2499 1684
Fragments arranged by size:
2499 1684 406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1639 1853 2155 2536 1639
Size: 214 302 381 3692
Fragments arranged by size:
3692 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1476 2881 2935 4419 4584 50
Size: 1426 1405 54 1484 165 55
Fragments arranged by size:
1484 1426 1405 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2501 2501
Size: 4589
BsrFI r'CCGG_y
Cuts at: 293 1024 2587 2768 2977 293
Size: 731 1563 181 209 1905
Fragments arranged by size:
1905 1563 731 209 181
BssSI C'ACGA_G
Cuts at: 2860 3218 2860
Size: 358 4231
BstAPI GCAn_nnn'nTGC
Cuts at: 984 1966 984
Size: 982 3607
BstBI TT'CG_AA
Cuts at: 110 2950 110
Size: 2840 1749
BstEII G'GTnAC_C
Cuts at: 1176 1176
Size: 4589
BstXI CCAn_nnnn'nTGG
Cuts at: 264 922 1153 1365 264
Size: 658 231 212 3488
Fragments arranged by size:
3488 658 231 212
BstYI r'GATC_y
Cuts at: 1 353 738 789 971 1614 2081 2104 2439
Size: 352 385 51 182 643 467 23 335
Cuts at: 2439 2685 3697 3783 3795 4141 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 643 467 449 385 352 346 335
246 182 86 51 23 12
BstZ17I GTA'TAC
Cuts at: 1301 1301
Size: 4589
BtgI C'CryG_G
Cuts at: 125 875 999 125
Size: 750 124 3715
Fragments arranged by size:
3715 750 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 415 2539 2720 2883 415
Size: 2124 181 163 2121
Fragments arranged by size:
2124 2121 181 163
BtsI GCAGTG_nn'
Cuts at: 1832 1832
Size: 4589
DraI TTT'AAA
Cuts at: 1588 1588
Size: 4589
DrdI GACnn_nn'nnGTC
Cuts at: 418 2295 3153 418
Size: 1877 858 1854
Fragments arranged by size:
1877 1854 858
EaeI y'GGCC_r
Cuts at: 924 1002 1085 1367 2174 2348 2739 2766 4111
Size: 78 83 282 807 174 391 27 1345
Cuts at: 4111 924
Size: 1402
Fragments arranged by size:
1402 1345 807 391 282 174 83 78
27
EagI C'GGCC_G
Cuts at: 2174 2174
Size: 4589
EarI CTCTTCn'nnn_
Cuts at: 572 641 913 1293 1304 1313 2612 2822 572
Size: 69 272 380 11 9 1299 210 2339
Fragments arranged by size:
2339 1299 380 272 210 69 11 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2797 3107 3253 4399 2797
Size: 310 146 1146 2987
Fragments arranged by size:
2987 1146 310 146
EcoICRI GAG'CTC
Cuts at: 223 680 1071 1601 223
Size: 457 391 530 3211
Fragments arranged by size:
3211 530 457 391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 666 1501 2414 2846 3593 3861 622
Size: 44 835 913 432 747 268 1350
Fragments arranged by size:
1350 913 835 747 432 268 44
EcoNI CCTnn'n_nnAGG
Cuts at: 343 343
Size: 4589
EcoO109I rG'GnC_Cy
Cuts at: 1125 1189 1418 1728 1125
Size: 64 229 310 3986
Fragments arranged by size:
3986 310 229 64
EcoRI G'AATT_C
Cuts at: 1593 1593
Size: 4589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1410 2608 1410
Size: 1198 3391
FspI TGC'GCA
Cuts at: 2370 2370
Size: 4589
HaeII r_GCGC'y
Cuts at: 800 2023 2271 3293 4272 800
Size: 1223 248 1022 979 1117
Fragments arranged by size:
1223 1117 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 175 180 207 730 762 1330 1362 2431
Size: 27 5 27 523 32 568 32 1069
Cuts at: 2431 2461 2463 2493 148
Size: 30 2 30 2244
Fragments arranged by size:
2244 1069 568 523 32 32 30 30
27 27 5 2
HincII GTy'rAC
Cuts at: 1628 1628
Size: 4589
KasI G'GCGC_C
Cuts at: 796 2019 2267 796
Size: 1223 248 3118
Fragments arranged by size:
3118 1223 248
KpnI G_GTAC'C
Cuts at: 1609 1609
Size: 4589
MluI A'CGCG_T
Cuts at: 4022 4022
Size: 4589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 786 3260 3444 786
Size: 2474 184 1931
Fragments arranged by size:
2474 1931 184
MscI TGG'CCA
Cuts at: 926 1087 1369 2350 926
Size: 161 282 981 3165
Fragments arranged by size:
3165 981 282 161
MslI CAynn'nnrTG
Cuts at: 262 1093 1166 2578 2705 262
Size: 831 73 1412 127 2146
Fragments arranged by size:
2146 1412 831 127 73
MspA1I CmG'CkG
Cuts at: 376 690 984 1122 1700 2014 2374 3387 3632
Size: 314 294 138 578 314 360 1013 245
Cuts at: 3632 376
Size: 1333
Fragments arranged by size:
1333 1013 578 360 314 314 294 245
138
NaeI GCC'GGC
Cuts at: 2770 2770
Size: 4589
NarI GG'CG_CC
Cuts at: 797 2020 2268 797
Size: 1223 248 3118
Fragments arranged by size:
3118 1223 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4589
NdeI CA'TA_TG
Cuts at: 750 750
Size: 4589
NgoMIV G'CCGG_C
Cuts at: 2768 2768
Size: 4589
NspI r_CATG'y
Cuts at: 1642 2142 3049 1642
Size: 500 907 3182
Fragments arranged by size:
3182 907 500
PasI CC'CwG_GG
Cuts at: 734 734
Size: 4589
PciI A'CATG_T
Cuts at: 3045 3045
Size: 4589
PflMI CCAn_nnn'nTGG
Cuts at: 793 793
Size: 4589
PmeI GTTT'AAAC
Cuts at: 1588 1588
Size: 4589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 3728
Size: 32 4557
PpuMI rG'GwC_Cy
Cuts at: 1125 1189 1125
Size: 64 4525
PstI C_TGCA'G
Cuts at: 989 1057 1384 1542 1636 2321 989
Size: 68 327 158 94 685 3257
Fragments arranged by size:
3257 685 327 158 94 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4589
PvuII CAG'CTG
Cuts at: 376 690 984 2014 2374 376
Size: 314 294 1030 360 2591
Fragments arranged by size:
2591 1030 360 314 294
RsrII CG'GwC_CG
Cuts at: 2784 2784
Size: 4589
SacI G_AGCT'C
Cuts at: 225 682 1073 1603 225
Size: 457 391 530 3211
Fragments arranged by size:
3211 530 457 391
SalI G'TCGA_C
Cuts at: 1626 1626
Size: 4589
SanDI GG'GwC_CC
Cuts at: 1125 1125
Size: 4589
SapI GCTCTTCn'nnn_
Cuts at: 572 2612 2822 572
Size: 2040 210 2339
Fragments arranged by size:
2339 2040 210
SbfI CC_TGCA'GG
Cuts at: 1636 1636
Size: 4589
ScaI AGT'ACT
Cuts at: 4106 4106
Size: 4589
SfcI C'TryA_G
Cuts at: 33 210 985 1053 1380 1538 1632 2317 3310
Size: 177 775 68 327 158 94 685 993
Cuts at: 3310 3501 33
Size: 191 1121
Fragments arranged by size:
1121 993 775 685 327 191 177 158
94 68
SfoI GGC'GCC
Cuts at: 798 2021 2269 798
Size: 1223 248 3118
Fragments arranged by size:
3118 1223 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4589
SmaI CCC'GGG
Cuts at: 1611 1611
Size: 4589
SmlI C'TyrA_G
Cuts at: 224 1744 2659 3151 3413 224
Size: 1520 915 492 262 1400
Fragments arranged by size:
1520 1400 915 492 262
SnaBI TAC'GTA
Cuts at: 1581 1581
Size: 4589
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4589
SphI G_CATG'C
Cuts at: 1642 2142 1642
Size: 500 4089
StyI C'CwwG_G
Cuts at: 125 490 1723 2077 125
Size: 365 1233 354 2637
Fragments arranged by size:
2637 1233 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 456 2172 2305 2764 2973 445
Size: 11 1716 133 459 209 2061
Fragments arranged by size:
2061 1716 459 209 133 11
TatI w'GTAC_w
Cuts at: 4104 4104
Size: 4589
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1000 1032 4106 4138 1000
Size: 32 3074 32 1451
Fragments arranged by size:
3074 1451 32 32
Tth111I GACn'n_nGTC
Cuts at: 2386 2386
Size: 4589
XbaI T'CTAG_A
Cuts at: 1620 1620
Size: 4589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1101 1431 2084 1101
Size: 330 653 3606
Fragments arranged by size:
3606 653 330
XmaI C'CCGG_G
Cuts at: 1609 1609
Size: 4589
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI