Restriction map for
pF1KSDA1735
(Circular) (Six-base) MAPSORT of: pF1KSDA1735.seq Check: 4072 from: 1 to: 4553
pF1KSDA1735 4553 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1591 1591
Size: 4553
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 458 634 778 986 1056 2378 2810 3557
Size: 198 176 144 208 70 1322 432 747
Cuts at: 3557 3825 260
Size: 268 988
Fragments arranged by size:
1322 988 747 432 268 208 198 176
144 70
AflIII A'CryG_T
Cuts at: 1905 3009 3986 1905
Size: 1104 977 2472
Fragments arranged by size:
2472 1104 977
AgeI A'CCGG_T
Cuts at: 1316 1412 2941 1316
Size: 96 1529 2928
Fragments arranged by size:
2928 1529 96
AlwNI CAG_nnn'CTG
Cuts at: 1289 1858 3425 1289
Size: 569 1567 2417
Fragments arranged by size:
2417 1567 569
ApaLI G'TGCA_C
Cuts at: 3323 3323
Size: 4553
ApoI r'AATT_y
Cuts at: 1557 3906 4162 1557
Size: 2349 256 1948
Fragments arranged by size:
2349 1948 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 284 487 1573 284
Size: 203 1086 3264
Fragments arranged by size:
3264 1086 203
BamHI G'GATC_C
Cuts at: 427 1434 1578 427
Size: 1007 144 3402
Fragments arranged by size:
3402 1007 144
BanI G'GyrC_C
Cuts at: 1569 1983 2231 2266 1569
Size: 414 248 35 3856
Fragments arranged by size:
3856 414 248 35
BanII G_rGCy'C
Cuts at: 307 1270 1567 2597 307
Size: 963 297 1030 2263
Fragments arranged by size:
2263 1030 963 297
BbeI G_GCGC'C
Cuts at: 1987 2235 1987
Size: 248 4305
BbsI GAAGACnn'nnnn_
Cuts at: 1532 1532
Size: 4553
BbvCI CC'TCA_GC
Cuts at: 1088 1088
Size: 4553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 766 800 1021 1055 766
Size: 34 221 34 4264
Fragments arranged by size:
4264 221 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2451 3212 4553 2451
Size: 761 1341 2451
Fragments arranged by size:
2451 1341 761
BclI T'GATC_A
Cuts at: 2073 2073
Size: 4553
BglII A'GATC_T
Cuts at: 2068 4105 2068
Size: 2037 2516
BlpI GC'TnA_GC
Cuts at: 1665 1665
Size: 4553
Bme1580I G_kGCm'C
Cuts at: 2178 2271 3327 2178
Size: 93 1056 3404
Fragments arranged by size:
3404 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 155 195 1765 1846 2181 4325 155
Size: 40 1570 81 335 2144 383
Fragments arranged by size:
2144 1570 383 335 81 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 644 1066 2920 644
Size: 422 1854 2277
Fragments arranged by size:
2277 1854 422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 340
Size: 4553
Bpu10I CC'TnA_GC
Cuts at: 1088 1088
Size: 4553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1729 2608 3100 3398 1190
Size: 539 879 492 298 2345
Fragments arranged by size:
2345 879 539 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 316 4257 316
Size: 3941 612
BsaAI yAC'GTr
Cuts at: 1545 1906 1545
Size: 361 4192
BsaBI GATnn'nnATC
Cuts at: 6 505 2067 4104 6
Size: 499 1562 2037 455
Fragments arranged by size:
2037 1562 499 455
BsaHI Gr'CG_yC
Cuts at: 1984 2232 2934 4430 1984
Size: 248 702 1496 2107
Fragments arranged by size:
2107 1496 702 248
BsaWI w'CCGG_w
Cuts at: 827 1316 1412 1742 2263 2941 3215 3362 3773
Size: 489 96 330 521 678 274 147 411
Cuts at: 3773 827
Size: 1607
Fragments arranged by size:
1607 678 521 489 411 330 274 147
96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 258 288 1354 1384 258
Size: 30 1066 30 3427
Fragments arranged by size:
3427 1066 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 1375 1474 550
Size: 825 99 3629
Fragments arranged by size:
3629 825 99
BseYI C'CCAG_C
Cuts at: 771 1978 2736 3313 3809 771
Size: 1207 758 577 496 1515
Fragments arranged by size:
1515 1207 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 4553
BsiEI CG_ry'CG
Cuts at: 104 2095 2141 3349 104
Size: 1991 46 1208 1308
Fragments arranged by size:
1991 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1270 1304 1567 2345 2535 3327 1270
Size: 34 263 778 190 792 2496
Fragments arranged by size:
2496 792 778 263 190 34
BsmBI CGTCTCn'nnnn_
Cuts at: 1150 1150
Size: 4553
Bsp1286I G_dGCh'C
Cuts at: 307 1270 1304 1567 2178 2271 2345 2535 2597
Size: 963 34 263 611 93 74 190 62
Cuts at: 2597 3327 307
Size: 730 1533
Fragments arranged by size:
1533 963 730 611 263 190 93 74
62 34
BspEI T'CCGG_A
Cuts at: 827 3773 827
Size: 2946 1607
BspHI T'CATG_A
Cuts at: 3729 3729
Size: 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1037 1603 1817 2119 2500 1037
Size: 566 214 302 381 3090
Fragments arranged by size:
3090 566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2845 2899 4383 4548 50
Size: 2795 54 1484 165 55
Fragments arranged by size:
2795 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 969 2465 969
Size: 1496 3057
BsrFI r'CCGG_y
Cuts at: 743 1316 1412 2551 2732 2941 743
Size: 573 96 1139 181 209 2355
Fragments arranged by size:
2355 1139 573 209 181 96
BssSI C'ACGA_G
Cuts at: 2824 3182 2824
Size: 358 4195
BstAPI GCAn_nnn'nTGC
Cuts at: 1930 1930
Size: 4553
BstBI TT'CG_AA
Cuts at: 110 2914 110
Size: 2804 1749
BstYI r'GATC_y
Cuts at: 1 427 714 861 1434 1578 2045 2068 2403
Size: 426 287 147 573 144 467 23 335
Cuts at: 2403 2649 3661 3747 3759 4105 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 573 467 449 426 346 335 287
246 147 144 86 23 12
BtgI C'CryG_G
Cuts at: 125 1530 125
Size: 1405 3148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2503 2684 2847 2503
Size: 181 163 4209
Fragments arranged by size:
4209 181 163
BtsI GCAGTG_nn'
Cuts at: 1796 1796
Size: 4553
DraI TTT'AAA
Cuts at: 1383 1552 1383
Size: 169 4384
DraIII CAC_nnn'GTG
Cuts at: 858 858
Size: 4553
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3117 2259
Size: 858 3695
EaeI y'GGCC_r
Cuts at: 295 1077 2138 2312 2703 2730 4075 295
Size: 782 1061 174 391 27 1345 773
Fragments arranged by size:
1345 1061 782 773 391 174 27
EagI C'GGCC_G
Cuts at: 2138 2138
Size: 4553
EarI CTCTTCn'nnn_
Cuts at: 203 2576 2786 203
Size: 2373 210 1970
Fragments arranged by size:
2373 1970 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2761 3071 3217 4363 2761
Size: 310 146 1146 2951
Fragments arranged by size:
2951 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1268 1565 1268
Size: 297 4256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 340 458 634 778 986 1056 2378 2810
Size: 80 118 176 144 208 70 1322 432
Cuts at: 2810 3557 3825 260
Size: 747 268 988
Fragments arranged by size:
1322 988 747 432 268 208 176 144
118 80 70
EcoO109I rG'GnC_Cy
Cuts at: 455 1692 455
Size: 1237 3316
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
EcoRV GAT'ATC
Cuts at: 398 398
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 627 2572 627
Size: 1945 2608
FspI TGC'GCA
Cuts at: 1063 2334 1063
Size: 1271 3282
HaeII r_GCGC'y
Cuts at: 1143 1987 2235 3257 4236 1143
Size: 844 248 1022 979 1460
Fragments arranged by size:
1460 1022 979 844 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 258 290 2395 2425 2427 2457 258
Size: 32 2105 30 2 30 2354
Fragments arranged by size:
2354 2105 32 30 30 2
HincII GTy'rAC
Cuts at: 1592 1592
Size: 4553
KasI G'GCGC_C
Cuts at: 1983 2231 1983
Size: 248 4305
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 1003 1297 1337 3224 3408 326
Size: 677 294 40 1887 184 1471
Fragments arranged by size:
1887 1471 677 294 184 40
MscI TGG'CCA
Cuts at: 1079 2314 1079
Size: 1235 3318
MslI CAynn'nnrTG
Cuts at: 887 1475 2542 2669 887
Size: 588 1067 127 2771
Fragments arranged by size:
2771 1067 588 127
MspA1I CmG'CkG
Cuts at: 238 342 1664 1978 2338 3351 3596 238
Size: 104 1322 314 360 1013 245 1195
Fragments arranged by size:
1322 1195 1013 360 314 245 104
NaeI GCC'GGC
Cuts at: 2734 2734
Size: 4553
NarI GG'CG_CC
Cuts at: 1984 2232 1984
Size: 248 4305
NcoI C'CATG_G
Cuts at: 125 1530 125
Size: 1405 3148
NgoMIV G'CCGG_C
Cuts at: 2732 2732
Size: 4553
NspI r_CATG'y
Cuts at: 1150 1606 2106 3013 1150
Size: 456 500 907 2690
Fragments arranged by size:
2690 907 500 456
PciI A'CATG_T
Cuts at: 3009 3009
Size: 4553
PflMI CCAn_nnn'nTGG
Cuts at: 711 858 711
Size: 147 4406
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4521
PshAI GACnn'nnGTC
Cuts at: 139 139
Size: 4553
PsiI TTA'TAA
Cuts at: 196 505 196
Size: 309 4244
PstI C_TGCA'G
Cuts at: 722 1034 1600 2285 722
Size: 312 566 685 2990
Fragments arranged by size:
2990 685 566 312
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 342 1978 2338 342
Size: 1636 360 2557
Fragments arranged by size:
2557 1636 360
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 1270 1567 1270
Size: 297 4256
SalI G'TCGA_C
Cuts at: 1590 1590
Size: 4553
SapI GCTCTTCn'nnn_
Cuts at: 2576 2786 2576
Size: 210 4343
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 4070 4070
Size: 4553
SexAI A'CCwGG_T
Cuts at: 474 854 474
Size: 380 4173
SfcI C'TryA_G
Cuts at: 33 406 718 1030 1596 2281 3274 3465 33
Size: 373 312 312 566 685 993 191 1121
Fragments arranged by size:
1121 993 685 566 373 312 312 191
SfoI GGC'GCC
Cuts at: 1985 2233 1985
Size: 248 4305
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SmaI CCC'GGG
Cuts at: 1575 1575
Size: 4553
SmlI C'TyrA_G
Cuts at: 284 1205 1708 2623 3115 3377 284
Size: 921 503 915 492 262 1460
Fragments arranged by size:
1460 921 915 503 492 262
SnaBI TAC'GTA
Cuts at: 1545 1545
Size: 4553
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 1606 2106 1606
Size: 500 4053
SspI AAT'ATT
Cuts at: 361 361
Size: 4553
StuI AGG'CCT
Cuts at: 412 412
Size: 4553
StyI C'CwwG_G
Cuts at: 125 458 1530 1687 2041 125
Size: 333 1072 157 354 2637
Fragments arranged by size:
2637 1072 354 333 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 174 1212 1506 2136 2269 2728 2937 174
Size: 1038 294 630 133 459 209 1790
Fragments arranged by size:
1790 1038 630 459 294 209 133
TatI w'GTAC_w
Cuts at: 1291 4068 1291
Size: 2777 1776
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4070 4102 4070
Size: 32 4521
Tth111I GACn'n_nGTC
Cuts at: 2350 2350
Size: 4553
XbaI T'CTAG_A
Cuts at: 864 1584 864
Size: 720 3833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2048 2048
Size: 4553
XhoI C'TCGA_G
Cuts at: 284 284
Size: 4553
XmaI C'CCGG_G
Cuts at: 1573 1573
Size: 4553
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BglI BmgBI BmtI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SwaI XmnI ZraI