Restriction map for
pF1KSDA1736
(Circular) (Six-base) MAPSORT of: pF1KSDA1736.seq Check: 5359 from: 1 to: 6851
pF1KSDA1736 6851 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 624 3889 624
Size: 3265 3586
Acc65I G'GTAC_C
Cuts at: 3655 3867 3655
Size: 212 6639
AclI AA'CG_TT
Cuts at: 6691 6691
Size: 6851
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 1221 1502 1606 1648 2865 3199 3256 4676
Size: 930 281 104 42 1217 334 57 1420
Cuts at: 4676 5108 5855 6123 291
Size: 432 747 268 1019
Fragments arranged by size:
1420 1217 1019 930 747 432 334 281
268 104 57 42
AfeI AGC'GCT
Cuts at: 3732 3732
Size: 6851
AflIII A'CryG_T
Cuts at: 2967 4203 5307 6284 2967
Size: 1236 1104 977 3534
Fragments arranged by size:
3534 1236 1104 977
AgeI A'CCGG_T
Cuts at: 5239 5239
Size: 6851
AhdI GACnn_n'nnGTC
Cuts at: 1003 2356 1003
Size: 1353 5498
AleI CACnn'nnGTG
Cuts at: 340 1784 3238 340
Size: 1444 1454 3953
Fragments arranged by size:
3953 1454 1444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 935 1987 935
Size: 1052 5799
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 746 778 746
Size: 32 6819
AlwNI CAG_nnn'CTG
Cuts at: 219 579 1064 1375 1671 1683 2655 3264 3340
Size: 360 485 311 296 12 972 609 76
Cuts at: 3340 3405 4156 5723 219
Size: 65 751 1567 1347
Fragments arranged by size:
1567 1347 972 751 609 485 360 311
296 76 65 12
ApaLI G'TGCA_C
Cuts at: 5621 5621
Size: 6851
ApoI r'AATT_y
Cuts at: 2102 3855 6204 6460 2102
Size: 1753 2349 256 2493
Fragments arranged by size:
2493 2349 1753 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6851
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6851
AvaI C'yCGr_G
Cuts at: 185 1076 1599 1981 3578 3871 185
Size: 891 523 382 1597 293 3165
Fragments arranged by size:
3165 1597 891 523 382 293
AvrII C'CTAG_G
Cuts at: 3817 3817
Size: 6851
BamHI G'GATC_C
Cuts at: 1043 2248 2837 3395 3876 1043
Size: 1205 589 558 481 4018
Fragments arranged by size:
4018 1205 589 558 481
BanI G'GyrC_C
Cuts at: 2306 3253 3309 3367 3541 3655 3867 4281 4529
Size: 947 56 58 174 114 212 414 248
Cuts at: 4529 4564 2306
Size: 35 4593
Fragments arranged by size:
4593 947 414 248 212 174 114 58
56 35
BanII G_rGCy'C
Cuts at: 201 1169 2176 2316 3865 4895 201
Size: 968 1007 140 1549 1030 2157
Fragments arranged by size:
2157 1549 1030 1007 968 140
BbeI G_GCGC'C
Cuts at: 4285 4533 4285
Size: 248 6603
BbsI GAAGACnn'nnnn_
Cuts at: 379 655 2871 3105 379
Size: 276 2216 234 4125
Fragments arranged by size:
4125 2216 276 234
BbvCI CC'TCA_GC
Cuts at: 950 3734 950
Size: 2784 4067
BciVI GTATCCnnnnn_n'
Cuts at: 152 4749 5510 6851 152
Size: 4597 761 1341 152
Fragments arranged by size:
4597 1341 761 152
BclI T'GATC_A
Cuts at: 4371 4371
Size: 6851
BglI GCCn_nnn'nGGC
Cuts at: 553 2684 553
Size: 2131 4720
BglII A'GATC_T
Cuts at: 2122 2609 4366 6403 2122
Size: 487 1757 2037 2570
Fragments arranged by size:
2570 2037 1757 487
BlpI GC'TnA_GC
Cuts at: 200 572 3388 3963 200
Size: 372 2816 575 3088
Fragments arranged by size:
3088 2816 575 372
Bme1580I G_kGCm'C
Cuts at: 807 3256 3532 4476 4569 5625 807
Size: 2449 276 944 93 1056 2033
Fragments arranged by size:
2449 2033 1056 944 276 93
BmrI ACTGGGnnnn_n'
Cuts at: 2103 2106 3099 4063 4144 4479 6623 2103
Size: 3 993 964 81 335 2144 2331
Fragments arranged by size:
2331 2144 993 964 335 81 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1206 1812 2190 5218 1206
Size: 606 378 3028 2839
Fragments arranged by size:
3028 2839 606 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 1569 1669 1995 2041 2515 639
Size: 930 100 326 46 474 4975
Fragments arranged by size:
4975 930 474 326 100 46
Bpu10I CC'TnA_GC
Cuts at: 950 2999 3622 3734 950
Size: 2049 623 112 4067
Fragments arranged by size:
4067 2049 623 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4906 5398 5696 4027
Size: 879 492 298 5182
Fragments arranged by size:
5182 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2902 3032 6555 2902
Size: 130 3523 3198
Fragments arranged by size:
3523 3198 130
BsaAI yAC'GTr
Cuts at: 3843 4204 3843
Size: 361 6490
BsaBI GATnn'nnATC
Cuts at: 6 4365 6402 6
Size: 4359 2037 455
Fragments arranged by size:
4359 2037 455
BsaHI Gr'CG_yC
Cuts at: 835 4282 4530 5232 6728 835
Size: 3447 248 702 1496 958
Fragments arranged by size:
3447 1496 958 702 248
BsaWI w'CCGG_w
Cuts at: 155 4040 4561 5239 5513 5660 6071 155
Size: 3885 521 678 274 147 411 935
Fragments arranged by size:
3885 935 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3029 3059 3731 3761 3029
Size: 30 672 30 6119
Fragments arranged by size:
6119 672 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 832 1890 2065 2182 2194 2211 2934 3546 832
Size: 1058 175 117 12 17 723 612 4137
Fragments arranged by size:
4137 1058 723 612 175 117 17 12
BseYI C'CCAG_C
Cuts at: 1874 1933 2005 2277 2398 2671 3563 3677 3727
Size: 59 72 272 121 273 892 114 50
Cuts at: 3727 4276 5034 5611 6107 1874
Size: 549 758 577 496 2618
Fragments arranged by size:
2618 892 758 577 549 496 273 272
121 114 72 59 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 2321 2617 2830 3457 841
Size: 1480 296 213 627 4235
Fragments arranged by size:
4235 1480 627 296 213
BsiEI CG_ry'CG
Cuts at: 104 1228 4393 4439 5647 104
Size: 1124 3165 46 1208 1308
Fragments arranged by size:
3165 1308 1208 1124 46
BsiHKAI G_wGCw'C
Cuts at: 186 201 2176 2261 2316 2864 3454 3865 4643
Size: 15 1975 85 55 548 590 411 778
Cuts at: 4643 4833 5625 186
Size: 190 792 1412
Fragments arranged by size:
1975 1412 792 778 590 548 411 190
85 55 15
BsmI GAATG_Cn'
Cuts at: 153 1607 3207 3288 3506 153
Size: 1454 1600 81 218 3498
Fragments arranged by size:
3498 1600 1454 218 81
BsmBI CGTCTCn'nnnn_
Cuts at: 1629 3100 1629
Size: 1471 5380
Bsp1286I G_dGCh'C
Cuts at: 186 201 807 1169 2176 2261 2316 2864 3256
Size: 15 606 362 1007 85 55 548 392
Cuts at: 3256 3454 3532 3865 4476 4569 4643 4833 4895
Size: 198 78 333 611 93 74 190 62
Cuts at: 4895 5625 186
Size: 730 1412
Fragments arranged by size:
1412 1007 730 611 606 548 392 362
333 198 190 93 85 78 74 62
55 15
BspEI T'CCGG_A
Cuts at: 155 6071 155
Size: 5916 935
BspHI T'CATG_A
Cuts at: 2409 6027 2409
Size: 3618 3233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1917 3901 4115 4417 4798 1917
Size: 1984 214 302 381 3970
Fragments arranged by size:
3970 1984 381 302 214
BsrBI CCG'CTC
Cuts at: 50 875 1304 1412 1986 3147 5143 5197 6681
Size: 825 429 108 574 1161 1996 54 1484
Cuts at: 6681 6846 50
Size: 165 55
Fragments arranged by size:
1996 1484 1161 825 574 429 165 108
55 54
BsrDI GCAATG_nn'
Cuts at: 542 2328 2507 4763 542
Size: 1786 179 2256 2630
Fragments arranged by size:
2630 2256 1786 179
BsrFI r'CCGG_y
Cuts at: 1223 1261 1658 2525 4849 5030 5239 1223
Size: 38 397 867 2324 181 209 2835
Fragments arranged by size:
2835 2324 867 397 209 181 38
BsrGI T'GTAC_A
Cuts at: 1155 1511 1155
Size: 356 6495
BssSI C'ACGA_G
Cuts at: 5122 5480 5122
Size: 358 6493
BstAPI GCAn_nnn'nTGC
Cuts at: 1064 4228 1064
Size: 3164 3687
BstBI TT'CG_AA
Cuts at: 110 5212 110
Size: 5102 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1039 2019 1039
Size: 980 5871
BstYI r'GATC_y
Cuts at: 1 602 761 1043 1372 2122 2248 2299 2609
Size: 601 159 282 329 750 126 51 310
Cuts at: 2609 2837 3395 3876 4343 4366 4701 4947 5959
Size: 228 558 481 467 23 335 246 1012
Cuts at: 5959 6045 6057 6403 1
Size: 86 12 346 449
Fragments arranged by size:
1012 750 601 558 481 467 449 346
335 329 310 282 246 228 159 126
86 51 23 12
Bsu36I CC'TnA_GG
Cuts at: 2165 3333 2165
Size: 1168 5683
BtgI C'CryG_G
Cuts at: 191 418 959 1809 2898 3431 3458 3467 3829
Size: 227 541 850 1089 533 27 9 362
Cuts at: 3829 191
Size: 3213
Fragments arranged by size:
3213 1089 850 541 533 362 227 27
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4801 4982 5145 4801
Size: 181 163 6507
Fragments arranged by size:
6507 181 163
BtsI GCAGTG_nn'
Cuts at: 516 590 1256 1791 2477 2717 3750 4094 516
Size: 74 666 535 686 240 1033 344 3273
Fragments arranged by size:
3273 1033 686 666 535 344 240 74
DraI TTT'AAA
Cuts at: 3850 3850
Size: 6851
DraIII CAC_nnn'GTG
Cuts at: 2868 2868
Size: 6851
DrdI GACnn_nn'nnGTC
Cuts at: 4557 5415 4557
Size: 858 5993
EaeI y'GGCC_r
Cuts at: 1225 1812 2498 2646 4436 4610 5001 5028 6373
Size: 587 686 148 1790 174 391 27 1345
Cuts at: 6373 1225
Size: 1703
Fragments arranged by size:
1790 1703 1345 686 587 391 174 148
27
EagI C'GGCC_G
Cuts at: 1225 4436 1225
Size: 3211 3640
EarI CTCTTCn'nnn_
Cuts at: 882 1458 2448 3699 4874 5084 882
Size: 576 990 1251 1175 210 2649
Fragments arranged by size:
2649 1251 1175 990 576 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1435 3427 5059 5369 5515 6661 1435
Size: 1992 1632 310 146 1146 1625
Fragments arranged by size:
1992 1632 1625 1146 310 146
EcoICRI GAG'CTC
Cuts at: 199 2174 2314 3863 199
Size: 1975 140 1549 3187
Fragments arranged by size:
3187 1975 1549 140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 639 1221 1502 1569 1606 1648 1669 1995
Size: 348 582 281 67 37 42 21 326
Cuts at: 1995 2041 2515 2865 3199 3256 4676 5108 5855
Size: 46 474 350 334 57 1420 432 747
Cuts at: 5855 6123 291
Size: 268 1019
Fragments arranged by size:
1420 1019 747 582 474 432 350 348
334 326 281 268 67 57 46 42
37 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2696 2832 2916 3002 3344 2696
Size: 136 84 86 342 6203
Fragments arranged by size:
6203 342 136 86 84
EcoO109I rG'GnC_Cy
Cuts at: 1212 1291 1315 1756 1929 2457 3337 3814 3990
Size: 79 24 441 173 528 880 477 176
Cuts at: 3990 1212
Size: 4073
Fragments arranged by size:
4073 880 528 477 441 176 173 79
24
EcoRI G'AATT_C
Cuts at: 3855 3855
Size: 6851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4870 4870
Size: 6851
FspI TGC'GCA
Cuts at: 4632 4632
Size: 6851
HaeII r_GCGC'y
Cuts at: 969 3734 4285 4533 5555 6534 969
Size: 2765 551 248 1022 979 1286
Fragments arranged by size:
2765 1286 1022 979 551 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 675 707 989 1021 1408 1440 2342 2374 2473
Size: 32 282 32 387 32 902 32 99
Cuts at: 2473 2505 3731 3763 4693 4723 4725 4755 675
Size: 32 1226 32 930 30 2 30 2771
Fragments arranged by size:
2771 1226 930 902 387 282 99 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 625 726 1196 1367 2451 3761 3890 625
Size: 101 470 171 1084 1310 129 3586
Fragments arranged by size:
3586 1310 1084 470 171 129 101
HpaI GTT'AAC
Cuts at: 726 2451 726
Size: 1725 5126
KasI G'GCGC_C
Cuts at: 4281 4529 4281
Size: 248 6603
KpnI G_GTAC'C
Cuts at: 3659 3871 3659
Size: 212 6639
MfeI C'AATT_G
Cuts at: 719 719
Size: 6851
MluI A'CGCG_T
Cuts at: 6284 6284
Size: 6851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1020 1823 3152 5522 5706 1020
Size: 803 1329 2370 184 2165
Fragments arranged by size:
2370 2165 1329 803 184
MscI TGG'CCA
Cuts at: 1814 2500 2648 4612 1814
Size: 686 148 1964 4053
Fragments arranged by size:
4053 1964 686 148
MslI CAynn'nnrTG
Cuts at: 340 889 1784 2414 2782 3238 3457 4840 4967
Size: 549 895 630 368 456 219 1383 127
Cuts at: 4967 340
Size: 2224
Fragments arranged by size:
2224 1383 895 630 549 456 368 219
127
MspA1I CmG'CkG
Cuts at: 204 1002 1021 1064 2627 2961 3099 3276 3441
Size: 798 19 43 1563 334 138 177 165
Cuts at: 3441 3962 4276 4636 5649 5894 204
Size: 521 314 360 1013 245 1161
Fragments arranged by size:
1563 1161 1013 798 521 360 334 314
245 177 165 138 43 19
NaeI GCC'GGC
Cuts at: 1263 5032 1263
Size: 3769 3082
NarI GG'CG_CC
Cuts at: 4282 4530 4282
Size: 248 6603
NcoI C'CATG_G
Cuts at: 191 418 2898 3458 3467 191
Size: 227 2480 560 9 3575
Fragments arranged by size:
3575 2480 560 227 9
NgoMIV G'CCGG_C
Cuts at: 1261 5030 1261
Size: 3769 3082
NspI r_CATG'y
Cuts at: 864 2971 3904 4404 5311 864
Size: 2107 933 500 907 2404
Fragments arranged by size:
2404 2107 933 907 500
PasI CC'CwG_GG
Cuts at: 3661 3661
Size: 6851
PciI A'CATG_T
Cuts at: 2967 5307 2967
Size: 2340 4511
PflMI CCAn_nnn'nTGG
Cuts at: 1960 2655 2736 3010 3264 3780 1960
Size: 695 81 274 254 516 5031
Fragments arranged by size:
5031 695 516 274 254 81
PmeI GTTT'AAAC
Cuts at: 3850 3850
Size: 6851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1396 1428 5990 6022 1396
Size: 32 4562 32 2225
Fragments arranged by size:
4562 2225 32 32
PpuMI rG'GwC_Cy
Cuts at: 1291 1929 2457 3337 1291
Size: 638 528 880 4805
Fragments arranged by size:
4805 880 638 528
PshAI GACnn'nnGTC
Cuts at: 620 620
Size: 6851
PspXI vC'TCGA_Gb
Cuts at: 1076 1076
Size: 6851
PstI C_TGCA'G
Cuts at: 215 2643 3281 3813 3898 4583 215
Size: 2428 638 532 85 685 2483
Fragments arranged by size:
2483 2428 685 638 532 85
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6851
PvuII CAG'CTG
Cuts at: 1021 2627 3099 3276 4276 4636 1021
Size: 1606 472 177 1000 360 3236
Fragments arranged by size:
3236 1606 1000 472 360 177
RsrII CG'GwC_CG
Cuts at: 5046 5046
Size: 6851
SacI G_AGCT'C
Cuts at: 201 2176 2316 3865 201
Size: 1975 140 1549 3187
Fragments arranged by size:
3187 1975 1549 140
SalI G'TCGA_C
Cuts at: 623 3888 623
Size: 3265 3586
SapI GCTCTTCn'nnn_
Cuts at: 882 4874 5084 882
Size: 3992 210 2649
Fragments arranged by size:
3992 2649 210
SbfI CC_TGCA'GG
Cuts at: 3898 3898
Size: 6851
ScaI AGT'ACT
Cuts at: 1394 2386 3085 6368 1394
Size: 992 699 3283 1877
Fragments arranged by size:
3283 1877 992 699
SexAI A'CCwGG_T
Cuts at: 3041 3537 3041
Size: 496 6355
SfcI C'TryA_G
Cuts at: 33 211 2639 3277 3809 3894 4579 5572 5763
Size: 178 2428 638 532 85 685 993 191
Cuts at: 5763 33
Size: 1121
Fragments arranged by size:
2428 1121 993 685 638 532 191 178
85
SfoI GGC'GCC
Cuts at: 4283 4531 4283
Size: 248 6603
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6851
SmaI CCC'GGG
Cuts at: 3873 3873
Size: 6851
SmlI C'TyrA_G
Cuts at: 1076 1981 4006 4921 5413 5675 1076
Size: 905 2025 915 492 262 2252
Fragments arranged by size:
2252 2025 915 905 492 262
SnaBI TAC'GTA
Cuts at: 3843 3843
Size: 6851
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6851
SphI G_CATG'C
Cuts at: 3904 4404 3904
Size: 500 6351
StuI AGG'CCT
Cuts at: 452 1189 2146 3064 3620 452
Size: 737 957 918 556 3683
Fragments arranged by size:
3683 957 918 737 556
StyI C'CwwG_G
Cuts at: 191 418 1038 2362 2898 3458 3467 3817 3985
Size: 227 620 1324 536 560 9 350 168
Cuts at: 3985 4339 191
Size: 354 2703
Fragments arranged by size:
2703 1324 620 560 536 354 350 227
168 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 352 1177 2125 2276 3271 4434 4567 5026 5235
Size: 825 948 151 995 1163 133 459 209
Cuts at: 5235 352
Size: 1968
Fragments arranged by size:
1968 1163 995 948 825 459 209 151
133
TatI w'GTAC_w
Cuts at: 1155 1240 1392 1511 2384 2714 3083 6366 1155
Size: 85 152 119 873 330 369 3283 1640
Fragments arranged by size:
3283 1640 873 369 330 152 119 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 420 452 850 882 1065 1097 2822 2854 3029
Size: 32 398 32 183 32 1725 32 175
Cuts at: 3029 3061 6368 6400 420
Size: 32 3307 32 871
Fragments arranged by size:
3307 1725 871 398 183 175 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1108 4648 1108
Size: 3540 3311
XbaI T'CTAG_A
Cuts at: 3882 3882
Size: 6851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 2862 3465 3781 4346 549
Size: 2313 603 316 565 3054
Fragments arranged by size:
3054 2313 603 565 316
XhoI C'TCGA_G
Cuts at: 1076 1981 1076
Size: 905 5946
XmaI C'CCGG_G
Cuts at: 3871 3871
Size: 6851
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII ApaI AscI BaeI BcgI BfrBI
BmgBI BmtI BsiWI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PsiI PspOMI PsrI SacII
SanDI SfiI SgrAI SrfI SspI SwaI XmnI ZraI