Restriction map for
pF1KSDA1753
(Circular) (Six-base) MAPSORT of: pF1KSDA1753.seq Check: 1163 from: 1 to: 5567
pF1KSDA1753 5567 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 313 2241 313
Size: 1928 3639
AatII G_ACGT'C
Cuts at: 389 389
Size: 5567
AccI GT'mk_AC
Cuts at: 389 605 2605 389
Size: 216 2000 3351
Fragments arranged by size:
3351 2000 216
Acc65I G'GTAC_C
Cuts at: 482 2583 482
Size: 2101 3466
AclI AA'CG_TT
Cuts at: 5407 5407
Size: 5567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1468 3392 3824 4571 4839 607
Size: 861 1924 432 747 268 1335
Fragments arranged by size:
1924 1335 861 747 432 268
AflII C'TTAA_G
Cuts at: 2434 2434
Size: 5567
AflIII A'CryG_T
Cuts at: 227 2919 4023 5000 227
Size: 2692 1104 977 794
Fragments arranged by size:
2692 1104 977 794
AgeI A'CCGG_T
Cuts at: 3955 3955
Size: 5567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 767 1045 1801 1811 2252 767
Size: 278 756 10 441 4082
Fragments arranged by size:
4082 756 441 278 10
AlwNI CAG_nnn'CTG
Cuts at: 195 1479 1988 2056 2310 2872 4439 195
Size: 1284 509 68 254 562 1567 1323
Fragments arranged by size:
1567 1323 1284 562 509 254 68
ApaI G_GGCC'C
Cuts at: 139 146 584 2382 2541 139
Size: 7 438 1798 159 3165
Fragments arranged by size:
3165 1798 438 159 7
ApaLI G'TGCA_C
Cuts at: 4337 4337
Size: 5567
ApoI r'AATT_y
Cuts at: 241 1416 2571 4920 5176 241
Size: 1175 1155 2349 256 632
Fragments arranged by size:
2349 1175 1155 632 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5567
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
AvaI C'yCGr_G
Cuts at: 139 208 997 1052 2019 2222 2587 139
Size: 69 789 55 967 203 365 3119
Fragments arranged by size:
3119 967 789 365 203 69 55
AvrII C'CTAG_G
Cuts at: 1251 1251
Size: 5567
BamHI G'GATC_C
Cuts at: 2592 2592
Size: 5567
BanI G'GyrC_C
Cuts at: 168 335 364 482 1185 1594 2203 2583 2997
Size: 167 29 118 703 409 609 380 414
Cuts at: 2997 3245 3280 168
Size: 248 35 2455
Fragments arranged by size:
2455 703 609 414 409 380 248 167
118 35 29
BanII G_rGCy'C
Cuts at: 139 146 584 865 1198 1291 1460 1795 2303
Size: 7 438 281 333 93 169 335 508
Cuts at: 2303 2382 2495 2541 2581 3611 139
Size: 79 113 46 40 1030 2095
Fragments arranged by size:
2095 1030 508 438 335 333 281 169
113 93 79 46 40 7
BbeI G_GCGC'C
Cuts at: 172 339 2207 3001 3249 172
Size: 167 1868 794 248 2490
Fragments arranged by size:
2490 1868 794 248 167
BbsI GAAGACnn'nnnn_
Cuts at: 1317 1394 1732 1317
Size: 77 338 5152
Fragments arranged by size:
5152 338 77
BbvCI CC'TCA_GC
Cuts at: 724 1119 1941 2501 724
Size: 395 822 560 3790
Fragments arranged by size:
3790 822 560 395
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 146 180 1752 1786 146
Size: 34 1572 34 3927
Fragments arranged by size:
3927 1572 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3465 4226 5567 3465
Size: 761 1341 3465
Fragments arranged by size:
3465 1341 761
BclI T'GATC_A
Cuts at: 3087 3087
Size: 5567
BglI GCCn_nnn'nGGC
Cuts at: 431 634 741 801 815 1592 2065 2143 2197
Size: 203 107 60 14 777 473 78 54
Cuts at: 2197 2531 431
Size: 334 3467
Fragments arranged by size:
3467 777 473 334 203 107 78 60
54 14
BglII A'GATC_T
Cuts at: 1001 3082 5119 1001
Size: 2081 2037 1449
Fragments arranged by size:
2081 2037 1449
BlpI GC'TnA_GC
Cuts at: 2679 2679
Size: 5567
Bme1580I G_kGCm'C
Cuts at: 139 146 262 445 584 1230 1597 1751 2026
Size: 7 116 183 139 646 367 154 275
Cuts at: 2026 2382 2518 2541 3192 3285 4341 139
Size: 356 136 23 651 93 1056 1365
Fragments arranged by size:
1365 1056 651 646 367 356 275 183
154 139 136 116 93 23 7
BmrI ACTGGGnnnn_n'
Cuts at: 1582 2779 2860 3195 5339 1582
Size: 1197 81 335 2144 1810
Fragments arranged by size:
2144 1810 1197 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 546 2355 3934 546
Size: 1809 1579 2179
Fragments arranged by size:
2179 1809 1579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1185 1333 1545 1741 2248 2263 2296 2347 2350
Size: 148 212 196 507 15 33 51 3
Cuts at: 2350 2533 1185
Size: 183 4219
Fragments arranged by size:
4219 507 212 196 183 148 51 33
15 3
Bpu10I CC'TnA_GC
Cuts at: 188 724 1119 1941 2297 2501 188
Size: 536 395 822 356 204 3254
Fragments arranged by size:
3254 822 536 395 356 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1521 2743 3622 4114 4412 1521
Size: 1222 879 492 298 2676
Fragments arranged by size:
2676 1222 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 920 5271 920
Size: 4351 1216
BsaAI yAC'GTr
Cuts at: 230 1080 2559 2920 230
Size: 850 1479 361 2877
Fragments arranged by size:
2877 1479 850 361
BsaBI GATnn'nnATC
Cuts at: 6 3081 5118 6
Size: 3075 2037 455
Fragments arranged by size:
3075 2037 455
BsaHI Gr'CG_yC
Cuts at: 169 336 386 953 2204 2998 3246 3948 5444
Size: 167 50 567 1251 794 248 702 1496
Cuts at: 5444 169
Size: 292
Fragments arranged by size:
1496 1251 794 702 567 292 248 167
50
BsaWI w'CCGG_w
Cuts at: 2756 3277 3955 4229 4376 4787 2756
Size: 521 678 274 147 411 3536
Fragments arranged by size:
3536 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 580 610 724 754 1169 1199 580
Size: 30 114 30 415 30 4948
Fragments arranged by size:
4948 415 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 745 1222 1866 2134 2188 2284 745
Size: 477 644 268 54 96 4028
Fragments arranged by size:
4028 644 477 268 96 54
BseYI C'CCAG_C
Cuts at: 688 762 1139 1503 2992 3750 4327 4823 688
Size: 74 377 364 1489 758 577 496 1432
Fragments arranged by size:
1489 1432 758 577 496 377 364 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 378 436 528 544 2269 378
Size: 58 92 16 1725 3676
Fragments arranged by size:
3676 1725 92 58 16
BsiEI CG_ry'CG
Cuts at: 104 179 3109 3155 4363 104
Size: 75 2930 46 1208 1308
Fragments arranged by size:
2930 1308 1208 75 46
BsiHKAI G_wGCw'C
Cuts at: 1795 2495 2581 3359 3549 4341 1795
Size: 700 86 778 190 792 3021
Fragments arranged by size:
3021 792 778 700 190 86
BsiWI C'GTAC_G
Cuts at: 2555 2555
Size: 5567
BsmI GAATG_Cn'
Cuts at: 1623 1623
Size: 5567
BsmBI CGTCTCn'nnnn_
Cuts at: 893 944 893
Size: 51 5516
Bsp1286I G_dGCh'C
Cuts at: 139 146 262 445 584 865 1198 1230 1291
Size: 7 116 183 139 281 333 32 61
Cuts at: 1291 1460 1597 1751 1795 2026 2303 2382 2495
Size: 169 137 154 44 231 277 79 113
Cuts at: 2495 2518 2541 2581 3192 3285 3359 3549 3611
Size: 23 23 40 611 93 74 190 62
Cuts at: 3611 4341 139
Size: 730 1365
Fragments arranged by size:
1365 730 611 333 281 277 231 190
183 169 154 139 137 116 113 93
79 74 62 61 44 40 32 23
23 7
BspEI T'CCGG_A
Cuts at: 4787 4787
Size: 5567
BspHI T'CATG_A
Cuts at: 4743 4743
Size: 5567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 313 470 1115 2241 2617 2831 3133 3514 313
Size: 157 645 1126 376 214 302 381 2366
Fragments arranged by size:
2366 1126 645 381 376 302 214 157
BsrBI CCG'CTC
Cuts at: 50 187 596 2187 2280 3859 3913 5397 5562
Size: 137 409 1591 93 1579 54 1484 165
Cuts at: 5562 50
Size: 55
Fragments arranged by size:
1591 1579 1484 409 165 137 93 55
54
BsrDI GCAATG_nn'
Cuts at: 3479 3479
Size: 5567
BsrFI r'CCGG_y
Cuts at: 341 851 3565 3746 3955 341
Size: 510 2714 181 209 1953
Fragments arranged by size:
2714 1953 510 209 181
BsrGI T'GTAC_A
Cuts at: 1157 1157
Size: 5567
BssSI C'ACGA_G
Cuts at: 520 3838 4196 520
Size: 3318 358 1891
Fragments arranged by size:
3318 1891 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1479 1887 2513 2944 1479
Size: 408 626 431 4102
Fragments arranged by size:
4102 626 431 408
BstBI TT'CG_AA
Cuts at: 110 3928 110
Size: 3818 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 688 688
Size: 5567
BstYI r'GATC_y
Cuts at: 1 719 1001 1954 2592 3059 3082 3417 3663
Size: 718 282 953 638 467 23 335 246
Cuts at: 3663 4675 4761 4773 5119 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 953 718 638 467 449 346 335
282 246 86 23 12
BtgI C'CryG_G
Cuts at: 576 629 656 799 576
Size: 53 27 143 5344
Fragments arranged by size:
5344 143 53 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3517 3698 3861 3517
Size: 181 163 5223
Fragments arranged by size:
5223 181 163
BtsI GCAGTG_nn'
Cuts at: 262 561 2119 2465 2810 262
Size: 299 1558 346 345 3019
Fragments arranged by size:
3019 1558 346 345 299
DraI TTT'AAA
Cuts at: 2566 2566
Size: 5567
DraIII CAC_nnn'GTG
Cuts at: 324 1019 324
Size: 695 4872
DrdI GACnn_nn'nnGTC
Cuts at: 3273 4131 3273
Size: 858 4709
EaeI y'GGCC_r
Cuts at: 176 648 1712 2532 3152 3326 3717 3744 5089
Size: 472 1064 820 620 174 391 27 1345
Cuts at: 5089 176
Size: 654
Fragments arranged by size:
1345 1064 820 654 620 472 391 174
27
EagI C'GGCC_G
Cuts at: 176 3152 176
Size: 2976 2591
EarI CTCTTCn'nnn_
Cuts at: 1551 3590 3800 1551
Size: 2039 210 3318
Fragments arranged by size:
3318 2039 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 340 873 2185 3775 4085 4231 5377 340
Size: 533 1312 1590 310 146 1146 530
Fragments arranged by size:
1590 1312 1146 533 530 310 146
EcoICRI GAG'CTC
Cuts at: 1793 2493 2579 1793
Size: 700 86 4781
Fragments arranged by size:
4781 700 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1185 1333 1468 1545 1741 2248 2263 2296
Size: 578 148 135 77 196 507 15 33
Cuts at: 2296 2347 2350 2533 3392 3824 4571 4839 607
Size: 51 3 183 859 432 747 268 1335
Fragments arranged by size:
1335 859 747 578 507 432 268 196
183 148 135 77 51 33 15 3
EcoNI CCTnn'n_nnAGG
Cuts at: 727 727
Size: 5567
EcoO109I rG'GnC_Cy
Cuts at: 135 136 581 1057 1147 2293 2307 2706 135
Size: 1 445 476 90 1146 14 399 2996
Fragments arranged by size:
2996 1146 476 445 399 90 14 1
EcoRI G'AATT_C
Cuts at: 241 2571 241
Size: 2330 3237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1562 3586 1562
Size: 2024 3543
FspI TGC'GCA
Cuts at: 3348 3348
Size: 5567
HaeII r_GCGC'y
Cuts at: 172 339 2207 2338 3001 3249 4271 5250 172
Size: 167 1868 131 663 248 1022 979 489
Fragments arranged by size:
1868 1022 979 663 489 248 167 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 667 699 3409 3439 3441 3471 580
Size: 32 55 32 2710 30 2 30 2676
Fragments arranged by size:
2710 2676 55 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2606 2606
Size: 5567
HindIII A'AGCT_T
Cuts at: 2010 2010
Size: 5567
KasI G'GCGC_C
Cuts at: 168 335 2203 2997 3245 168
Size: 167 1868 794 248 2490
Fragments arranged by size:
2490 1868 794 248 167
KpnI G_GTAC'C
Cuts at: 486 2587 486
Size: 2101 3466
MluI A'CGCG_T
Cuts at: 5000 5000
Size: 5567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1898 2348 4238 4422 1898
Size: 450 1890 184 3043
Fragments arranged by size:
3043 1890 450 184
MscI TGG'CCA
Cuts at: 650 3328 650
Size: 2678 2889
MslI CAynn'nnrTG
Cuts at: 914 3556 3683 914
Size: 2642 127 2798
Fragments arranged by size:
2798 2642 127
MspA1I CmG'CkG
Cuts at: 333 801 1046 1123 2232 2678 2992 3352 4365
Size: 468 245 77 1109 446 314 360 1013
Cuts at: 4365 4610 333
Size: 245 1290
Fragments arranged by size:
1290 1109 1013 468 446 360 314 245
245 77
NaeI GCC'GGC
Cuts at: 3748 3748
Size: 5567
NarI GG'CG_CC
Cuts at: 169 336 2204 2998 3246 169
Size: 167 1868 794 248 2490
Fragments arranged by size:
2490 1868 794 248 167
NcoI C'CATG_G
Cuts at: 629 629
Size: 5567
NgoMIV G'CCGG_C
Cuts at: 3746 3746
Size: 5567
NspI r_CATG'y
Cuts at: 1032 1164 1213 1503 2418 2620 3120 4027 1032
Size: 132 49 290 915 202 500 907 2572
Fragments arranged by size:
2572 915 907 500 290 202 132 49
PasI CC'CwG_GG
Cuts at: 1330 1899 1330
Size: 569 4998
PciI A'CATG_T
Cuts at: 4023 4023
Size: 5567
PfoI T'CCnGG_A
Cuts at: 2125 2264 2125
Size: 139 5428
PmeI GTTT'AAAC
Cuts at: 2566 2566
Size: 5567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 973 1005 4706 4738 973
Size: 32 3701 32 1802
Fragments arranged by size:
3701 1802 32 32
PpuMI rG'GwC_Cy
Cuts at: 1057 1147 1057
Size: 90 5477
PspOMI G'GGCC_C
Cuts at: 135 142 580 2378 2537 135
Size: 7 438 1798 159 3165
Fragments arranged by size:
3165 1798 438 159 7
PstI C_TGCA'G
Cuts at: 200 1112 1790 1892 2614 3299 200
Size: 912 678 102 722 685 2468
Fragments arranged by size:
2468 912 722 685 678 102
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5567
PvuII CAG'CTG
Cuts at: 1046 1123 2992 3352 1046
Size: 77 1869 360 3261
Fragments arranged by size:
3261 1869 360 77
RsrII CG'GwC_CG
Cuts at: 3762 3762
Size: 5567
SacI G_AGCT'C
Cuts at: 1795 2495 2581 1795
Size: 700 86 4781
Fragments arranged by size:
4781 700 86
SacII CC_GC'GG
Cuts at: 802 802
Size: 5567
SalI G'TCGA_C
Cuts at: 2604 2604
Size: 5567
SapI GCTCTTCn'nnn_
Cuts at: 3590 3800 3590
Size: 210 5357
SbfI CC_TGCA'GG
Cuts at: 2614 2614
Size: 5567
ScaI AGT'ACT
Cuts at: 964 5084 964
Size: 4120 1447
SexAI A'CCwGG_T
Cuts at: 1391 1391
Size: 5567
SfcI C'TryA_G
Cuts at: 33 196 413 1108 1236 1786 1888 2610 3295
Size: 163 217 695 128 550 102 722 685
Cuts at: 3295 4288 4479 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 722 695 685 550 217 191
163 128 102
SfiI GGCCn_nnn'nGGCC
Cuts at: 634 634
Size: 5567
SfoI GGC'GCC
Cuts at: 170 337 2205 2999 3247 170
Size: 167 1868 794 248 2490
Fragments arranged by size:
2490 1868 794 248 167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
SmaI CCC'GGG
Cuts at: 141 2021 2224 2589 141
Size: 1880 203 365 3119
Fragments arranged by size:
3119 1880 365 203
SmlI C'TyrA_G
Cuts at: 1536 2434 2722 3637 4129 4391 1536
Size: 898 288 915 492 262 2712
Fragments arranged by size:
2712 915 898 492 288 262
SnaBI TAC'GTA
Cuts at: 2559 2559
Size: 5567
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5567
SphI G_CATG'C
Cuts at: 1213 2620 3120 1213
Size: 1407 500 3660
Fragments arranged by size:
3660 1407 500
StuI AGG'CCT
Cuts at: 419 637 419
Size: 218 5349
StyI C'CwwG_G
Cuts at: 629 813 1251 1820 2701 3055 629
Size: 184 438 569 881 354 3141
Fragments arranged by size:
3141 881 569 438 354 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1153 1769 3150 3283 3742 3951 1153
Size: 616 1381 133 459 209 2769
Fragments arranged by size:
2769 1381 616 459 209 133
TatI w'GTAC_w
Cuts at: 962 1157 5082 962
Size: 195 3925 1447
Fragments arranged by size:
3925 1447 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 724 756 1169 1201 1834 1866 5084 5116 724
Size: 32 413 32 633 32 3218 32 1175
Fragments arranged by size:
3218 1175 633 413 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3364 3364
Size: 5567
XbaI T'CTAG_A
Cuts at: 2598 2598
Size: 5567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3062 3062
Size: 5567
XmaI C'CCGG_G
Cuts at: 139 2019 2222 2587 139
Size: 1880 203 365 3119
Fragments arranged by size:
3119 1880 365 203
XmnI GAAnn'nnTTC
Cuts at: 241 1417 1729 241
Size: 1176 312 4079
Fragments arranged by size:
4079 1176 312
ZraI GAC'GTC
Cuts at: 387 387
Size: 5567
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AleI AloI AscI BaeI BfrBI BmgBI
BmtI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspXI PsrI
SanDI SgrAI SrfI SspI SwaI XhoI