Restriction map for
pF1KSDA1769
(Circular) (Six-base) MAPSORT of: pF1KSDA1769.seq Check: 3801 from: 1 to: 9377
pF1KSDA1769 9377 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1057 3176 3399 3990 4059 5001 5446 1057
Size: 2119 223 591 69 942 445 4988
Fragments arranged by size:
4988 2119 942 591 445 223 69
AatII G_ACGT'C
Cuts at: 2663 2663
Size: 9377
AccI GT'mk_AC
Cuts at: 3815 4537 6415 3815
Size: 722 1878 6777
Fragments arranged by size:
6777 1878 722
Acc65I G'GTAC_C
Cuts at: 1802 3449 4107 4220 5657 6393 1802
Size: 1647 658 113 1437 736 4786
Fragments arranged by size:
4786 1647 1437 736 658 113
AclI AA'CG_TT
Cuts at: 9217 9217
Size: 9377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2023 2131 2758 3531 4128 4300 4410 5155 5770
Size: 108 627 773 597 172 110 745 615
Cuts at: 5770 7202 7634 8381 8649 2023
Size: 1432 432 747 268 2751
Fragments arranged by size:
2751 1432 773 747 745 627 615 597
432 268 172 110 108
AfeI AGC'GCT
Cuts at: 3349 3934 3349
Size: 585 8792
AflIII A'CryG_T
Cuts at: 794 2045 3422 6729 7833 8810 794
Size: 1251 1377 3307 1104 977 1361
Fragments arranged by size:
3307 1377 1361 1251 1104 977
AgeI A'CCGG_T
Cuts at: 4217 5639 7765 4217
Size: 1422 2126 5829
Fragments arranged by size:
5829 2126 1422
AleI CACnn'nnGTG
Cuts at: 2371 2539 2825 4325 2371
Size: 168 286 1500 7423
Fragments arranged by size:
7423 1500 286 168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2615 2843 3581 4105 5224 5281 2615
Size: 228 738 524 1119 57 6711
Fragments arranged by size:
6711 1119 738 524 228 57
AlwNI CAG_nnn'CTG
Cuts at: 435 881 954 1059 1241 1594 2294 2651 3480
Size: 446 73 105 182 353 700 357 829
Cuts at: 3480 3630 3912 4151 4323 4433 5455 5766 6682
Size: 150 282 239 172 110 1022 311 916
Cuts at: 6682 8249 435
Size: 1567 1563
Fragments arranged by size:
1567 1563 1022 916 829 700 446 357
353 311 282 239 182 172 150 110
105 73
ApaI G_GGCC'C
Cuts at: 546 1879 4209 4496 4942 6222 546
Size: 1333 2330 287 446 1280 3701
Fragments arranged by size:
3701 2330 1333 1280 446 287
ApaLI G'TGCA_C
Cuts at: 938 1042 2527 3051 4615 8147 938
Size: 104 1485 524 1564 3532 2168
Fragments arranged by size:
3532 2168 1564 1485 524 104
ApoI r'AATT_y
Cuts at: 6381 8730 8986 6381
Size: 2349 256 6772
Fragments arranged by size:
6772 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9377
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9377
AvaI C'yCGr_G
Cuts at: 205 545 1246 1307 1525 1720 1795 1872 1966
Size: 340 701 61 218 195 75 77 94
Cuts at: 1966 2823 3371 4040 4209 4399 4453 4489 5225
Size: 857 548 669 169 190 54 36 736
Cuts at: 5225 6397 205
Size: 1172 3185
Fragments arranged by size:
3185 1172 857 736 701 669 548 340
218 195 190 169 94 77 75 61
54 36
AvrII C'CTAG_G
Cuts at: 969 969
Size: 9377
BamHI G'GATC_C
Cuts at: 3829 5276 6402 3829
Size: 1447 1126 6804
Fragments arranged by size:
6804 1447 1126
BanI G'GyrC_C
Cuts at: 338 384 478 925 1521 1802 1962 2340 2464
Size: 46 94 447 596 281 160 378 124
Cuts at: 2464 2827 2912 2959 3138 3449 3806 4081 4107
Size: 363 85 47 179 311 357 275 26
Cuts at: 4107 4134 4220 4372 4675 5657 6393 6807 7055
Size: 27 86 152 303 982 736 414 248
Cuts at: 7055 7090 338
Size: 35 2625
Fragments arranged by size:
2625 982 736 596 447 414 378 363
357 311 303 281 275 248 179 160
152 124 94 86 85 47 46 35
27 26
BanII G_rGCy'C
Cuts at: 133 380 546 1438 1622 1796 1879 3505 3536
Size: 247 166 892 184 174 83 1626 31
Cuts at: 3536 3670 4117 4209 4496 4942 5224 5348 5963
Size: 134 447 92 287 446 282 124 615
Cuts at: 5963 6222 6233 6391 7421 133
Size: 259 11 158 1030 2089
Fragments arranged by size:
2089 1626 1030 892 615 447 446 287
282 259 247 184 174 166 158 134
124 92 83 31 11
BbeI G_GCGC'C
Cuts at: 1966 2468 3810 4376 6811 7059 1966
Size: 502 1342 566 2435 248 4284
Fragments arranged by size:
4284 2435 1342 566 502 248
BbsI GAAGACnn'nnnn_
Cuts at: 624 1520 4963 5758 624
Size: 896 3443 795 4243
Fragments arranged by size:
4243 3443 896 795
BbvCI CC'TCA_GC
Cuts at: 955 1580 2665 4118 4666 5165 5912 955
Size: 625 1085 1453 548 499 747 4420
Fragments arranged by size:
4420 1453 1085 747 625 548 499
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 468 502 894 928 2408 2442 4434 4468 468
Size: 34 392 34 1480 34 1992 34 5377
Fragments arranged by size:
5377 1992 1480 392 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7275 8036 9377 7275
Size: 761 1341 7275
Fragments arranged by size:
7275 1341 761
BclI T'GATC_A
Cuts at: 2810 6897 2810
Size: 4087 5290
BfrBI ATG'CAT
Cuts at: 5700 5700
Size: 9377
BglI GCCn_nnn'nGGC
Cuts at: 550 1011 1250 1537 1971 3500 3785 4019 4359
Size: 461 239 287 434 1529 285 234 340
Cuts at: 4359 5798 6046 550
Size: 1439 248 3881
Fragments arranged by size:
3881 1529 1439 461 434 340 287 285
248 239 234
BglII A'GATC_T
Cuts at: 2093 6892 8929 2093
Size: 4799 2037 2541
Fragments arranged by size:
4799 2541 2037
BlpI GC'TnA_GC
Cuts at: 247 1033 1063 6305 6489 247
Size: 786 30 5242 184 3135
Fragments arranged by size:
5242 3135 786 184 30
Bme1580I G_kGCm'C
Cuts at: 546 930 942 1046 1077 1526 1879 2345 2531
Size: 384 12 104 31 449 353 466 186
Cuts at: 2531 2558 2633 3055 3143 4209 4496 4619 4678
Size: 27 75 422 88 1066 287 123 59
Cuts at: 4678 4942 6222 7002 7095 8151 546
Size: 264 1280 780 93 1056 1772
Fragments arranged by size:
1772 1280 1066 1056 780 466 449 422
384 353 287 264 186 123 104 93
88 75 59 31 27 12
BmgBI CAC'GTC
Cuts at: 189 1518 2577 3459 3657 4230 5478 5756 189
Size: 1329 1059 882 198 573 1248 278 3810
Fragments arranged by size:
3810 1329 1248 1059 882 573 278 198
BmrI ACTGGGnnnn_n'
Cuts at: 256 1128 1347 4578 4792 5176 5774 6589 6670
Size: 872 219 3231 214 384 598 815 81
Cuts at: 6670 7005 9149 256
Size: 335 2144 484
Fragments arranged by size:
3231 2144 872 815 598 484 384 335
219 214 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3684 4320 5362 5367 6268 7744 3684
Size: 636 1042 5 901 1476 5317
Fragments arranged by size:
5317 1476 1042 901 636 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 2344 3364 3538 3934 4051 4177 4291 4405
Size: 1887 1020 174 396 117 126 114 114
Cuts at: 4405 4855 4933 5337 5920 457
Size: 450 78 404 583 3914
Fragments arranged by size:
3914 1887 1020 583 450 404 396 174
126 117 114 114 78
Bpu10I CC'TnA_GC
Cuts at: 379 955 1580 2665 4118 4666 5165 5212 5912
Size: 576 625 1085 1453 548 499 47 700
Cuts at: 5912 379
Size: 3844
Fragments arranged by size:
3844 1453 1085 700 625 576 548 499
47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2194 2355 3091 3601 6145 6553 7432 7924 8222
Size: 161 736 510 2544 408 879 492 298
Cuts at: 8222 2194
Size: 3349
Fragments arranged by size:
3349 2544 879 736 510 492 408 298
161
BsaI GGTCTCn'nnnn_
Cuts at: 1822 2492 5573 5648 6239 9081 1822
Size: 670 3081 75 591 2842 2118
Fragments arranged by size:
3081 2842 2118 670 591 75
BsaAI yAC'GTr
Cuts at: 693 771 2048 3425 4164 4535 5832 6369 6730
Size: 78 1277 1377 739 371 1297 537 361
Cuts at: 6730 693
Size: 3340
Fragments arranged by size:
3340 1377 1297 1277 739 537 371 361
78
BsaBI GATnn'nnATC
Cuts at: 6 6891 8928 6
Size: 6885 2037 455
Fragments arranged by size:
6885 2037 455
BsaHI Gr'CG_yC
Cuts at: 659 805 1100 1160 1634 1843 1963 2465 2660
Size: 146 295 60 474 209 120 502 195
Cuts at: 2660 2867 3807 3976 4342 4351 4373 4756 6808
Size: 207 940 169 366 9 22 383 2052
Cuts at: 6808 7056 7758 9254 659
Size: 248 702 1496 782
Fragments arranged by size:
2052 1496 940 782 702 502 474 383
366 295 248 209 207 195 169 146
120 60 22 9
BsaWI w'CCGG_w
Cuts at: 2144 4217 4653 5273 5639 6566 7087 7765 8039
Size: 2073 436 620 366 927 521 678 274
Cuts at: 8039 8186 8597 2144
Size: 147 411 2924
Fragments arranged by size:
2924 2073 927 678 620 521 436 411
366 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1490 1520 1852 1882 4002 4032 1490
Size: 30 332 30 2120 30 6835
Fragments arranged by size:
6835 2120 332 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 123 206 972 1390 1860 2809 2818 4417 4765
Size: 83 766 418 470 949 9 1599 348
Cuts at: 4765 5784 5917 123
Size: 1019 133 3583
Fragments arranged by size:
3583 1599 1019 949 766 470 418 348
133 83 9
BseYI C'CCAG_C
Cuts at: 874 881 1139 1321 1437 1992 2256 3489 3623
Size: 7 258 182 116 555 264 1233 134
Cuts at: 3623 3639 3694 3980 4047 4495 4822 4882 5049
Size: 16 55 286 67 448 327 60 167
Cuts at: 5049 6802 7560 8137 8633 874
Size: 1753 758 577 496 1618
Fragments arranged by size:
1753 1618 1233 758 577 555 496 448
327 286 264 258 182 167 134 116
67 60 55 16 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 636 871 1960 3136 3154 3190 3583 3631
Size: 83 235 1089 1176 18 36 393 48
Cuts at: 3631 4018 4024 4093 5035 5251 5418 5468 5563
Size: 387 6 69 942 216 167 50 95
Cuts at: 5563 5854 553
Size: 291 4076
Fragments arranged by size:
4076 1176 1089 942 393 387 291 235
216 167 95 83 69 50 48 36
18 6
BsiEI CG_ry'CG
Cuts at: 104 244 297 392 554 891 2415 2835 3363
Size: 140 53 95 162 337 1524 420 528
Cuts at: 3363 3784 4964 6919 6965 8173 104
Size: 421 1180 1955 46 1208 1308
Fragments arranged by size:
1955 1524 1308 1208 1180 528 421 420
337 162 140 95 53 46
BsiHKAI G_wGCw'C
Cuts at: 133 380 560 942 1034 1046 1112 1622 2531
Size: 247 180 382 92 12 66 510 909
Cuts at: 2531 3055 3761 4163 4619 5348 6106 6391 7169
Size: 524 706 402 456 729 758 285 778
Cuts at: 7169 7359 8151 133
Size: 190 792 1359
Fragments arranged by size:
1359 909 792 778 758 729 706 524
510 456 402 382 285 247 190 180
92 66 12
BsmBI CGTCTCn'nnnn_
Cuts at: 1284 1493 3700 3883 1284
Size: 209 2207 183 6778
Fragments arranged by size:
6778 2207 209 183
Bsp1286I G_dGCh'C
Cuts at: 133 380 546 560 930 942 1034 1046 1077
Size: 247 166 14 370 12 92 12 31
Cuts at: 1077 1112 1438 1526 1622 1796 1879 2345 2531
Size: 35 326 88 96 174 83 466 186
Cuts at: 2531 2558 2633 3055 3143 3505 3536 3670 3761
Size: 27 75 422 88 362 31 134 91
Cuts at: 3761 4117 4163 4209 4496 4619 4678 4942 5224
Size: 356 46 46 287 123 59 264 282
Cuts at: 5224 5348 5963 6106 6222 6233 6391 7002 7095
Size: 124 615 143 116 11 158 611 93
Cuts at: 7095 7169 7359 7421 8151 133
Size: 74 190 62 730 1359
Fragments arranged by size:
1359 730 615 611 466 422 370 362
356 326 287 282 264 247 190 186
174 166 158 143 134 124 123 116
96 93 92 91 88 88 83 75
74 62 59 46 46 35 31 31
27 14 12 12 11
BspEI T'CCGG_A
Cuts at: 2144 4653 8597 2144
Size: 2509 3944 2924
Fragments arranged by size:
3944 2924 2509
BspHI T'CATG_A
Cuts at: 8553 8553
Size: 9377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 501 825 1057 1257 1763 2115 3176 3222 3399
Size: 324 232 200 506 352 1061 46 177
Cuts at: 3399 3437 3990 4059 4817 5001 5446 6427 6641
Size: 38 553 69 758 184 445 981 214
Cuts at: 6641 6943 7324 501
Size: 302 381 2554
Fragments arranged by size:
2554 1061 981 758 553 506 445 381
352 324 302 232 214 200 184 177
69 46 38
BsrBI CCG'CTC
Cuts at: 50 756 1230 1546 1800 1839 4170 4839 5616
Size: 706 474 316 254 39 2331 669 777
Cuts at: 5616 5655 5847 7669 7723 9207 9372 50
Size: 39 192 1822 54 1484 165 55
Fragments arranged by size:
2331 1822 1484 777 706 669 474 316
254 192 165 55 54 39 39
BsrDI GCAATG_nn'
Cuts at: 7289 7289
Size: 9377
BsrFI r'CCGG_y
Cuts at: 239 387 2153 2204 2410 2640 2830 2844 2909
Size: 148 1766 51 206 230 190 14 65
Cuts at: 2909 3779 4217 4282 4592 4787 5414 5639 6325
Size: 870 438 65 310 195 627 225 686
Cuts at: 6325 7375 7556 7765 239
Size: 1050 181 209 1851
Fragments arranged by size:
1851 1766 1050 870 686 627 438 310
230 225 209 206 195 190 181 148
65 65 51 14
BsrGI T'GTAC_A
Cuts at: 230 5150 230
Size: 4920 4457
BssHII G'CGCG_C
Cuts at: 1785 3589 1785
Size: 1804 7573
BssSI C'ACGA_G
Cuts at: 487 604 637 2017 3022 5662 5842 6316 7648
Size: 117 33 1380 1005 2640 180 474 1332
Cuts at: 7648 8006 487
Size: 358 1858
Fragments arranged by size:
2640 1858 1380 1332 1005 474 358 180
117 33
BstAPI GCAn_nnn'nTGC
Cuts at: 564 2919 3050 3480 5455 6754 564
Size: 2355 131 430 1975 1299 3187
Fragments arranged by size:
3187 2355 1975 1299 430 131
BstBI TT'CG_AA
Cuts at: 110 7738 110
Size: 7628 1749
BstEII G'GTnAC_C
Cuts at: 351 1677 3282 4767 351
Size: 1326 1605 1485 4961
Fragments arranged by size:
4961 1605 1485 1326
BstXI CCAn_nnnn'nTGG
Cuts at: 1857 5787 1857
Size: 3930 5447
BstYI r'GATC_y
Cuts at: 1 998 2093 3476 3829 5276 5486 6402 6869
Size: 997 1095 1383 353 1447 210 916 467
Cuts at: 6869 6892 7227 7473 8485 8571 8583 8929 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1447 1383 1095 1012 997 916 467 449
353 346 335 246 210 86 23 12
BstZ17I GTA'TAC
Cuts at: 4538 4538
Size: 9377
Bsu36I CC'TnA_GG
Cuts at: 2282 5251 5767 2282
Size: 2969 516 5892
Fragments arranged by size:
5892 2969 516
BtgI C'CryG_G
Cuts at: 800 921 1163 1259 1400 1571 1606 2714 2762
Size: 121 242 96 141 171 35 1108 48
Cuts at: 2762 2861 3287 3495 3884 4472 4551 5034 5513
Size: 99 426 208 389 588 79 483 479
Cuts at: 5513 5864 800
Size: 351 4313
Fragments arranged by size:
4313 1108 588 483 479 426 389 351
242 208 171 141 121 99 96 79
48 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2105 4761 5955 6021 7327 7508 7671 2105
Size: 2656 1194 66 1306 181 163 3811
Fragments arranged by size:
3811 2656 1306 1194 181 163 66
BtsI GCAGTG_nn'
Cuts at: 153 1511 1809 3148 3847 4059 6620 153
Size: 1358 298 1339 699 212 2561 2910
Fragments arranged by size:
2910 2561 1358 1339 699 298 212
ClaI AT'CG_AT
Cuts at: 416 3527 416
Size: 3111 6266
DraI TTT'AAA
Cuts at: 6376 6376
Size: 9377
DraIII CAC_nnn'GTG
Cuts at: 345 609 345
Size: 264 9113
DrdI GACnn_nn'nnGTC
Cuts at: 664 2872 5974 7083 7941 664
Size: 2208 3102 1109 858 2100
Fragments arranged by size:
3102 2208 2100 1109 858
EaeI y'GGCC_r
Cuts at: 198 241 536 551 695 888 1370 1397 1603
Size: 43 295 15 144 193 482 27 206
Cuts at: 1603 2102 2400 2412 2543 2642 2679 2693 2730
Size: 499 298 12 131 99 37 14 37
Cuts at: 2730 2832 2879 3159 3360 3393 3605 3752 3781
Size: 102 47 280 201 33 212 147 29
Cuts at: 3781 3881 3937 4020 4277 4475 5510 5826 6047
Size: 100 56 83 257 198 1035 316 221
Cuts at: 6047 6327 6962 7136 7527 7554 8899 198
Size: 280 635 174 391 27 1345 676
Fragments arranged by size:
1345 1035 676 635 499 482 391 316
298 295 280 280 257 221 212 206
201 198 193 174 147 144 131 102
100 99 83 56 47 43 37 37
33 29 27 27 15 14 12
EagI C'GGCC_G
Cuts at: 241 551 888 2412 2832 3360 3781 6962 241
Size: 310 337 1524 420 528 421 3181 2656
Fragments arranged by size:
3181 2656 1524 528 421 420 337 310
EarI CTCTTCn'nnn_
Cuts at: 583 1999 2005 2215 2734 3694 4627 4652 5212
Size: 1416 6 210 519 960 933 25 560
Cuts at: 5212 5353 7400 7610 583
Size: 141 2047 210 2350
Fragments arranged by size:
2350 2047 1416 960 933 560 519 210
210 141 25 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 298 722 2815 4369 7585 7895 8041 9187 298
Size: 424 2093 1554 3216 310 146 1146 488
Fragments arranged by size:
3216 2093 1554 1146 488 424 310 146
EcoICRI GAG'CTC
Cuts at: 131 378 1620 5346 6389 131
Size: 247 1242 3726 1043 3119
Fragments arranged by size:
3726 3119 1242 1043 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 2023 2131 2344 2758 3364 3531 3538 3934
Size: 1566 108 213 414 606 167 7 396
Cuts at: 3934 4051 4128 4177 4291 4300 4405 4410 4855
Size: 117 77 49 114 9 105 5 445
Cuts at: 4855 4933 5155 5337 5770 5920 7202 7634 8381
Size: 78 222 182 433 150 1282 432 747
Cuts at: 8381 8649 457
Size: 268 1185
Fragments arranged by size:
1566 1282 1185 747 606 445 433 432
414 396 268 222 213 182 167 150
117 114 108 105 78 77 49 9
7 5
EcoNI CCTnn'n_nnAGG
Cuts at: 973 1307 1372 1732 2146 2653 3334 5902 973
Size: 334 65 360 414 507 681 2568 4448
Fragments arranged by size:
4448 2568 681 507 414 360 334 65
EcoO109I rG'GnC_Cy
Cuts at: 447 542 966 1184 1251 1365 1529 1876 2052
Size: 95 424 218 67 114 164 347 176
Cuts at: 2052 2115 2127 2304 2506 3111 3339 4206 4938
Size: 63 12 177 202 605 228 867 732
Cuts at: 4938 5026 5056 5286 5361 5763 6018 6516 447
Size: 88 30 230 75 402 255 498 3308
Fragments arranged by size:
3308 867 732 605 498 424 402 347
255 230 228 218 202 177 176 164
114 95 88 75 67 63 30 12
EcoRI G'AATT_C
Cuts at: 6381 6381
Size: 9377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3660 5400 7396 3660
Size: 1740 1996 5641
Fragments arranged by size:
5641 1996 1740
FseI GG_CCGG'CC
Cuts at: 3783 4791 3783
Size: 1008 8369
FspI TGC'GCA
Cuts at: 833 7158 833
Size: 6325 3052
HaeII r_GCGC'y
Cuts at: 1299 1966 2468 2600 3351 3490 3810 3936 4376
Size: 667 502 132 751 139 320 126 440
Cuts at: 4376 4584 6090 6811 7059 8081 9060 1299
Size: 208 1506 721 248 1022 979 1616
Fragments arranged by size:
1616 1506 1022 979 751 721 667 502
440 320 248 208 139 132 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1240 1272 1828 1852 1855 1860 1884 1887 4171
Size: 32 556 24 3 5 24 3 2284
Cuts at: 4171 4203 4741 4773 6020 6052 7219 7249 7251
Size: 32 538 32 1247 32 1167 30 2
Cuts at: 7251 7281 1240
Size: 30 3336
Fragments arranged by size:
3336 2284 1247 1167 556 538 32 32
32 32 30 30 24 24 5 3
3 2
HincII GTy'rAC
Cuts at: 4524 5496 6416 4524
Size: 972 920 7485
Fragments arranged by size:
7485 972 920
KasI G'GCGC_C
Cuts at: 1962 2464 3806 4372 6807 7055 1962
Size: 502 1342 566 2435 248 4284
Fragments arranged by size:
4284 2435 1342 566 502 248
KpnI G_GTAC'C
Cuts at: 1806 3453 4111 4224 5661 6397 1806
Size: 1647 658 113 1437 736 4786
Fragments arranged by size:
4786 1647 1437 736 658 113
MluI A'CGCG_T
Cuts at: 8810 8810
Size: 9377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2330 2951 5108 5285 8048 8232 2330
Size: 621 2157 177 2763 184 3475
Fragments arranged by size:
3475 2763 2157 621 184 177
MscI TGG'CCA
Cuts at: 538 697 2545 2695 2732 4022 5512 5828 7138
Size: 159 1848 150 37 1290 1490 316 1310
Cuts at: 7138 538
Size: 2777
Fragments arranged by size:
2777 1848 1490 1310 1290 316 159 150
37
MslI CAynn'nnrTG
Cuts at: 799 2371 2539 2825 2989 4325 4639 7366 7493
Size: 1572 168 286 164 1336 314 2727 127
Cuts at: 7493 799
Size: 2683
Fragments arranged by size:
2727 2683 1572 1336 314 286 168 164
127
MspA1I CmG'CkG
Cuts at: 194 246 459 650 878 1143 1330 1558 1857
Size: 52 213 191 228 265 187 228 299
Cuts at: 1857 2256 2736 2784 3365 3471 3582 3674 3750
Size: 399 480 48 581 106 111 92 76
Cuts at: 3750 3909 3984 4008 4365 4430 4764 5592 5937
Size: 159 75 24 357 65 334 828 345
Cuts at: 5937 6141 6488 6802 7162 8175 8420 194
Size: 204 347 314 360 1013 245 1151
Fragments arranged by size:
1151 1013 828 581 480 399 360 357
347 345 334 314 299 265 245 228
228 213 204 191 187 159 111 106
92 76 75 65 52 48 24
NaeI GCC'GGC
Cuts at: 2155 2206 2412 2642 2832 2846 2911 3781 4789
Size: 51 206 230 190 14 65 870 1008
Cuts at: 4789 6327 7558 2155
Size: 1538 1231 3974
Fragments arranged by size:
3974 1538 1231 1008 870 230 206 190
65 51 14
NarI GG'CG_CC
Cuts at: 1963 2465 3807 4373 6808 7056 1963
Size: 502 1342 566 2435 248 4284
Fragments arranged by size:
4284 2435 1342 566 502 248
NcoI C'CATG_G
Cuts at: 800 1259 1400 2714 3495 800
Size: 459 141 1314 781 6682
Fragments arranged by size:
6682 1314 781 459 141
NdeI CA'TA_TG
Cuts at: 5675 5675
Size: 9377
NgoMIV G'CCGG_C
Cuts at: 2153 2204 2410 2640 2830 2844 2909 3779 4787
Size: 51 206 230 190 14 65 870 1008
Cuts at: 4787 6325 7556 2153
Size: 1538 1231 3974
Fragments arranged by size:
3974 1538 1231 1008 870 230 206 190
65 51 14
NsiI A_TGCA'T
Cuts at: 5702 5702
Size: 9377
NspI r_CATG'y
Cuts at: 510 798 3300 5700 6430 6930 7837 510
Size: 288 2502 2400 730 500 907 2050
Fragments arranged by size:
2502 2400 2050 907 730 500 288
PasI CC'CwG_GG
Cuts at: 1069 2460 2558 2964 5943 1069
Size: 1391 98 406 2979 4503
Fragments arranged by size:
4503 2979 1391 406 98
PciI A'CATG_T
Cuts at: 794 7833 794
Size: 7039 2338
PflMI CCAn_nnn'nTGG
Cuts at: 435 881 1146 2294 2988 3630 3771 3853 4029
Size: 446 265 1148 694 642 141 82 176
Cuts at: 4029 4635 4893 6155 435
Size: 606 258 1262 3657
Fragments arranged by size:
3657 1262 1148 694 642 606 446 265
258 176 141 82
PfoI T'CCnGG_A
Cuts at: 167 2267 3959 4028 4832 5471 6287 167
Size: 2100 1692 69 804 639 816 3257
Fragments arranged by size:
3257 2100 1692 816 804 639 69
PmeI GTTT'AAAC
Cuts at: 6376 6376
Size: 9377
PmlI CAC'GTG
Cuts at: 771 2048 3425 4164 5832 771
Size: 1277 1377 739 1668 4316
Fragments arranged by size:
4316 1668 1377 1277 739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8516 8548 8516
Size: 32 9345
PpuMI rG'GwC_Cy
Cuts at: 966 1365 2052 2115 2127 2506 5056 5361 966
Size: 399 687 63 12 379 2550 305 4982
Fragments arranged by size:
4982 2550 687 399 379 305 63 12
PshAI GACnn'nnGTC
Cuts at: 2504 2504
Size: 9377
PspOMI G'GGCC_C
Cuts at: 542 1875 4205 4492 4938 6218 542
Size: 1333 2330 287 446 1280 3701
Fragments arranged by size:
3701 2330 1333 1280 446 287
PspXI vC'TCGA_Gb
Cuts at: 4399 4453 4399
Size: 54 9323
PstI C_TGCA'G
Cuts at: 275 1557 1733 2162 2381 3321 3410 3749 3932
Size: 1282 176 429 219 940 89 339 183
Cuts at: 3932 4070 4094 4370 4813 5336 6346 6424 7109
Size: 138 24 276 443 523 1010 78 685
Cuts at: 7109 275
Size: 2543
Fragments arranged by size:
2543 1282 1010 940 685 523 443 429
339 276 219 183 176 138 89 78
24
PvuI CG_AT'CG
Cuts at: 104 4964 104
Size: 4860 4517
PvuII CAG'CTG
Cuts at: 878 1143 2736 2784 3750 3909 4365 4430 5592
Size: 265 1593 48 966 159 456 65 1162
Cuts at: 5592 5937 6802 7162 878
Size: 345 865 360 3093
Fragments arranged by size:
3093 1593 1162 966 865 456 360 345
265 159 65 48
RsrII CG'GwC_CG
Cuts at: 237 2924 7572 237
Size: 2687 4648 2042
Fragments arranged by size:
4648 2687 2042
SacI G_AGCT'C
Cuts at: 133 380 1622 5348 6391 133
Size: 247 1242 3726 1043 3119
Fragments arranged by size:
3726 3119 1242 1043 247
SalI G'TCGA_C
Cuts at: 6414 6414
Size: 9377
SanDI GG'GwC_CC
Cuts at: 2052 2506 5056 2052
Size: 454 2550 6373
Fragments arranged by size:
6373 2550 454
SapI GCTCTTCn'nnn_
Cuts at: 2215 2734 3694 5212 5353 7400 7610 2215
Size: 519 960 1518 141 2047 210 3982
Fragments arranged by size:
3982 2047 1518 960 519 210 141
SbfI CC_TGCA'GG
Cuts at: 3321 4070 6424 3321
Size: 749 2354 6274
Fragments arranged by size:
6274 2354 749
ScaI AGT'ACT
Cuts at: 277 8894 277
Size: 8617 760
SexAI A'CCwGG_T
Cuts at: 4944 5363 4944
Size: 419 8958
SfcI C'TryA_G
Cuts at: 33 271 1553 1729 2136 2158 2377 3317 3406
Size: 238 1282 176 407 22 219 940 89
Cuts at: 3406 3745 3928 4066 4090 4366 4809 5332 6342
Size: 339 183 138 24 276 443 523 1010
Cuts at: 6342 6420 7105 8098 8289 33
Size: 78 685 993 191 1121
Fragments arranged by size:
1282 1121 1010 993 940 685 523 443
407 339 276 238 219 191 183 176
138 89 78 24 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 550 3785 550
Size: 3235 6142
SfoI GGC'GCC
Cuts at: 1964 2466 3808 4374 6809 7057 1964
Size: 502 1342 566 2435 248 4284
Fragments arranged by size:
4284 2435 1342 566 502 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9377
SgrAI Cr'CCGG_yG
Cuts at: 4282 4282
Size: 9377
SmaI CCC'GGG
Cuts at: 547 1527 2825 3373 4042 4491 5227 6399 547
Size: 980 1298 548 669 449 736 1172 3525
Fragments arranged by size:
3525 1298 1172 980 736 669 548 449
SmlI C'TyrA_G
Cuts at: 2173 2334 3106 3616 4399 4453 6160 6532 7447
Size: 161 772 510 783 54 1707 372 915
Cuts at: 7447 7939 8201 2173
Size: 492 262 3349
Fragments arranged by size:
3349 1707 915 783 772 510 492 372
262 161 54
SnaBI TAC'GTA
Cuts at: 6369 6369
Size: 9377
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9377
SphI G_CATG'C
Cuts at: 5700 6430 6930 5700
Size: 730 500 8147
Fragments arranged by size:
8147 730 500
SrfI GCCC'GGGC
Cuts at: 547 547
Size: 9377
StuI AGG'CCT
Cuts at: 859 3544 5317 6001 859
Size: 2685 1773 684 4235
Fragments arranged by size:
4235 2685 1773 684
StyI C'CwwG_G
Cuts at: 698 800 969 1259 1400 2369 2714 3495 5888
Size: 102 169 290 141 969 345 781 2393
Cuts at: 5888 6511 6865 698
Size: 623 354 3210
Fragments arranged by size:
3210 2393 969 781 623 354 345 290
169 141 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 304 3268 3317 4533 6960 7093 7552 7761 304
Size: 2964 49 1216 2427 133 459 209 1920
Fragments arranged by size:
2964 2427 1920 1216 459 209 133 49
TatI w'GTAC_w
Cuts at: 230 275 2327 2999 5069 5150 8892 230
Size: 45 2052 672 2070 81 3742 715
Fragments arranged by size:
3742 2070 2052 715 672 81 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1490 1522 4002 4034 8894 8926 1490
Size: 32 2480 32 4860 32 1941
Fragments arranged by size:
4860 2480 1941 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 262 1708 3280 4306 5365 7174 262
Size: 1446 1572 1026 1059 1809 2465
Fragments arranged by size:
2465 1809 1572 1446 1059 1026
XbaI T'CTAG_A
Cuts at: 6408 6408
Size: 9377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2097 2395 2517 4779 4830 4890 5091 5361 5895
Size: 298 122 2262 51 60 201 270 534
Cuts at: 5895 6129 6872 2097
Size: 234 743 4602
Fragments arranged by size:
4602 2262 743 534 298 270 234 201
122 60 51
XhoI C'TCGA_G
Cuts at: 4399 4453 4399
Size: 54 9323
XmaI C'CCGG_G
Cuts at: 545 1525 2823 3371 4040 4489 5225 6397 545
Size: 980 1298 548 669 449 736 1172 3525
Fragments arranged by size:
3525 1298 1172 980 736 669 548 449
XmnI GAAnn'nnTTC
Cuts at: 4921 4921
Size: 9377
ZraI GAC'GTC
Cuts at: 2661 2661
Size: 9377
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI BaeI BmtI BsiWI BsmI
CspCI EcoRV FspAI HindIII HpaI MfeI NheI NotI
NruI PacI PsiI PsrI SacII SspI SwaI