Restriction map for
pF1KSDA1778
(Circular) (Six-base) MAPSORT of: pF1KSDA1778.seq Check: 5403 from: 1 to: 4112
pF1KSDA1778 4112 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1030 1030
Size: 4112
AatII G_ACGT'C
Cuts at: 853 853
Size: 4112
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
AfeI AGC'GCT
Cuts at: 919 919
Size: 4112
AflIII A'CryG_T
Cuts at: 145 1464 2568 3545 145
Size: 1319 1104 977 712
Fragments arranged by size:
1319 1104 977 712
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AhdI GACnn_n'nnGTC
Cuts at: 975 975
Size: 4112
AleI CACnn'nnGTG
Cuts at: 280 280
Size: 4112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 4112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 796 828 796
Size: 32 4080
AlwNI CAG_nnn'CTG
Cuts at: 339 726 1417 2984 339
Size: 387 691 1567 1467
Fragments arranged by size:
1567 1467 691 387
ApaI G_GGCC'C
Cuts at: 703 703
Size: 4112
ApaLI G'TGCA_C
Cuts at: 562 2882 562
Size: 2320 1792
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 344 448 511 534 744 1132 344
Size: 104 63 23 210 388 3324
Fragments arranged by size:
3324 388 210 104 63 23
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 288 516 631 645 1018 1128 1542 1790 1825
Size: 228 115 14 373 110 414 248 35
Cuts at: 1825 288
Size: 2575
Fragments arranged by size:
2575 414 373 248 228 115 110 35
14
BanII G_rGCy'C
Cuts at: 500 577 617 703 1126 2156 500
Size: 77 40 86 423 1030 2456
Fragments arranged by size:
2456 1030 423 86 77 40
BbeI G_GCGC'C
Cuts at: 520 635 649 1546 1794 520
Size: 115 14 897 248 2838
Fragments arranged by size:
2838 897 248 115 14
BbsI GAAGACnn'nnnn_
Cuts at: 1080 1080
Size: 4112
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BglI GCCn_nnn'nGGC
Cuts at: 552 552
Size: 4112
BglII A'GATC_T
Cuts at: 1061 1627 3664 1061
Size: 566 2037 1509
Fragments arranged by size:
2037 1509 566
BlpI GC'TnA_GC
Cuts at: 1224 1224
Size: 4112
Bme1580I G_kGCm'C
Cuts at: 293 566 703 1021 1737 1830 2886 293
Size: 273 137 318 716 93 1056 1519
Fragments arranged by size:
1519 1056 716 318 273 137 93
BmrI ACTGGGnnnn_n'
Cuts at: 714 1324 1405 1740 3884 714
Size: 610 81 335 2144 942
Fragments arranged by size:
2144 942 610 335 81
BmtI G_CTAG'C
Cuts at: 674 674
Size: 4112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 651 2479 651
Size: 1828 2284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 976 682
Size: 294 3818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 1288 2167 2659 2957 925
Size: 363 879 492 298 2080
Fragments arranged by size:
2080 879 492 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 148 261 1104 1465 148
Size: 113 843 361 2795
Fragments arranged by size:
2795 843 361 113
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 517 632 646 806 850 1543 1791 2493 3989
Size: 115 14 160 44 693 248 702 1496
Cuts at: 3989 517
Size: 640
Fragments arranged by size:
1496 702 693 640 248 160 115 44
14
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 757 787 757
Size: 30 4082
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 180 180
Size: 4112
BseYI C'CCAG_C
Cuts at: 521 579 1537 2295 2872 3368 521
Size: 58 958 758 577 496 1265
Fragments arranged by size:
1265 958 758 577 496 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 1026 258
Size: 768 3344
BsiEI CG_ry'CG
Cuts at: 104 906 1654 1700 2908 104
Size: 802 748 46 1208 1308
Fragments arranged by size:
1308 1208 802 748 46
BsiHKAI G_wGCw'C
Cuts at: 500 566 1126 1904 2094 2886 500
Size: 66 560 778 190 792 1726
Fragments arranged by size:
1726 792 778 560 190 66
Bsp1286I G_dGCh'C
Cuts at: 293 500 566 577 617 703 1021 1126 1737
Size: 207 66 11 40 86 318 105 611
Cuts at: 1737 1830 1904 2094 2156 2886 293
Size: 93 74 190 62 730 1519
Fragments arranged by size:
1519 730 611 318 207 190 105 93
86 74 66 62 40 11
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 3288 3288
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 419 1030 1162 1376 1678 2059 419
Size: 611 132 214 302 381 2472
Fragments arranged by size:
2472 611 381 302 214 132
BsrBI CCG'CTC
Cuts at: 50 216 2404 2458 3942 4107 50
Size: 166 2188 54 1484 165 55
Fragments arranged by size:
2188 1484 166 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 458 737 2110 2291 2500 458
Size: 279 1373 181 209 2070
Fragments arranged by size:
2070 1373 279 209 181
BssHII G'CGCG_C
Cuts at: 826 826
Size: 4112
BssSI C'ACGA_G
Cuts at: 427 499 2383 2741 427
Size: 72 1884 358 1798
Fragments arranged by size:
1884 1798 358 72
BstAPI GCAn_nnn'nTGC
Cuts at: 429 1489 429
Size: 1060 3052
BstBI TT'CG_AA
Cuts at: 110 2473 110
Size: 2363 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 132 132
Size: 4112
BstYI r'GATC_y
Cuts at: 1 1061 1137 1604 1627 1962 2208 3220 3306
Size: 1060 76 467 23 335 246 1012 86
Cuts at: 3306 3318 3664 1
Size: 12 346 449
Fragments arranged by size:
1060 1012 467 449 346 335 246 86
76 23 12
BtgI C'CryG_G
Cuts at: 125 203 768 1013 125
Size: 78 565 245 3224
Fragments arranged by size:
3224 565 245 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 189 1355 189
Size: 1166 2946
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DraIII CAC_nnn'GTG
Cuts at: 999 999
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 857 1697 1871 2262 2289 3634 857
Size: 840 174 391 27 1345 1335
Fragments arranged by size:
1345 1335 840 391 174 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 223 394 2135 2345 223
Size: 171 1741 210 1990
Fragments arranged by size:
1990 1741 210 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 151 817 2320 2630 2776 3922 151
Size: 666 1503 310 146 1146 341
Fragments arranged by size:
1503 1146 666 341 310 146
EcoICRI GAG'CTC
Cuts at: 498 1124 498
Size: 626 3486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 976 1937 2369 3116 3384 682
Size: 294 961 432 747 268 1410
Fragments arranged by size:
1410 961 747 432 294 268
EcoNI CCTnn'n_nnAGG
Cuts at: 958 958
Size: 4112
EcoO109I rG'GnC_Cy
Cuts at: 897 1251 897
Size: 354 3758
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 966 2131 966
Size: 1165 2947
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 273 520 635 649 744 921 1546 1794 2816
Size: 247 115 14 95 177 625 248 1022
Cuts at: 2816 3795 273
Size: 979 590
Fragments arranged by size:
1022 979 625 590 248 247 177 115
95 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 961 993 1954 1984 1986 2016 961
Size: 32 961 30 2 30 3057
Fragments arranged by size:
3057 961 32 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 516 631 645 1542 1790 516
Size: 115 14 897 248 2838
Fragments arranged by size:
2838 897 248 115 14
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2783 2967 2783
Size: 184 3928
MscI TGG'CCA
Cuts at: 859 1873 859
Size: 1014 3098
MslI CAynn'nnrTG
Cuts at: 280 2101 2228 280
Size: 1821 127 2164
Fragments arranged by size:
2164 1821 127
MspA1I CmG'CkG
Cuts at: 205 705 770 1223 1537 1897 2910 3155 205
Size: 500 65 453 314 360 1013 245 1162
Fragments arranged by size:
1162 1013 500 453 360 314 245 65
NaeI GCC'GGC
Cuts at: 460 739 2293 460
Size: 279 1554 2279
Fragments arranged by size:
2279 1554 279
NarI GG'CG_CC
Cuts at: 517 632 646 1543 1791 517
Size: 115 14 897 248 2838
Fragments arranged by size:
2838 897 248 115 14
NcoI C'CATG_G
Cuts at: 125 125
Size: 4112
NgoMIV G'CCGG_C
Cuts at: 458 737 2291 458
Size: 279 1554 2279
Fragments arranged by size:
2279 1554 279
NheI G'CTAG_C
Cuts at: 670 670
Size: 4112
NspI r_CATG'y
Cuts at: 315 1165 1665 2572 315
Size: 850 500 907 1855
Fragments arranged by size:
1855 907 850 500
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PflMI CCAn_nnn'nTGG
Cuts at: 726 999 726
Size: 273 3839
PfoI T'CCnGG_A
Cuts at: 401 953 401
Size: 552 3560
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PmlI CAC'GTG
Cuts at: 148 148
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 3251 3283 114
Size: 32 3105 32 943
Fragments arranged by size:
3105 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 897 897
Size: 4112
PspOMI G'GGCC_C
Cuts at: 699 699
Size: 4112
PstI C_TGCA'G
Cuts at: 467 1096 1159 1844 467
Size: 629 63 685 2735
Fragments arranged by size:
2735 685 629 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 1537 1897 1537
Size: 360 3752
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 500 1126 500
Size: 626 3486
SacII CC_GC'GG
Cuts at: 206 771 206
Size: 565 3547
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SanDI GG'GwC_CC
Cuts at: 897 897
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 223 2135 2345 223
Size: 1912 210 1990
Fragments arranged by size:
1990 1912 210
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 481 3629 481
Size: 3148 964
SfcI C'TryA_G
Cuts at: 33 463 1092 1155 1840 2833 3024 33
Size: 430 629 63 685 993 191 1121
Fragments arranged by size:
1121 993 685 629 430 191 63
SfoI GGC'GCC
Cuts at: 518 633 647 1544 1792 518
Size: 115 14 897 248 2838
Fragments arranged by size:
2838 897 248 115 14
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 346 536 1134 346
Size: 190 598 3324
Fragments arranged by size:
3324 598 190
SmlI C'TyrA_G
Cuts at: 940 1267 2182 2674 2936 940
Size: 327 915 492 262 2116
Fragments arranged by size:
2116 915 492 327 262
SnaBI TAC'GTA
Cuts at: 1104 1104
Size: 4112
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StuI AGG'CCT
Cuts at: 964 964
Size: 4112
StyI C'CwwG_G
Cuts at: 125 860 1246 1600 125
Size: 735 386 354 2637
Fragments arranged by size:
2637 735 386 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 1695 1828 2287 2496 571
Size: 1124 133 459 209 2187
Fragments arranged by size:
2187 1124 459 209 133
TatI w'GTAC_w
Cuts at: 479 3627 479
Size: 3148 964
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 203 235 3629 3661 203
Size: 32 3394 32 654
Fragments arranged by size:
3394 654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1607 663
Size: 944 3168
XmaI C'CCGG_G
Cuts at: 344 534 1132 344
Size: 190 598 3324
Fragments arranged by size:
3324 598 190
ZraI GAC'GTC
Cuts at: 851 851
Size: 4112
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
Bpu10I BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PshAI PsiI
PspXI PsrI SexAI SfiI SgrAI SrfI SspI SwaI
XhoI XmnI