Restriction map for
pF1KSDA1783
(Circular) (Six-base) MAPSORT of: pF1KSDA1783.seq Check: 8668 from: 1 to: 12425
pF1KSDA1783 12425 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1044 4363 8534 8961 9251 1044
Size: 3319 4171 427 290 4218
Fragments arranged by size:
4218 4171 3319 427 290
AccI GT'mk_AC
Cuts at: 4331 8341 9463 4331
Size: 4010 1122 7293
Fragments arranged by size:
7293 4010 1122
Acc65I G'GTAC_C
Cuts at: 1315 3186 4424 9441 1315
Size: 1871 1238 5017 4299
Fragments arranged by size:
5017 4299 1871 1238
AclI AA'CG_TT
Cuts at: 12265 12265
Size: 12425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1832 3031 4636 5250 6194 7059 7426 7504 8224
Size: 1199 1605 614 944 865 367 78 720
Cuts at: 8224 8815 9241 10250 10682 11429 11697 1832
Size: 591 426 1009 432 747 268 2560
Fragments arranged by size:
2560 1605 1199 1009 944 865 747 720
614 591 432 426 367 268 78
AfeI AGC'GCT
Cuts at: 728 4315 5129 728
Size: 3587 814 8024
Fragments arranged by size:
8024 3587 814
AflII C'TTAA_G
Cuts at: 7561 7561
Size: 12425
AflIII A'CryG_T
Cuts at: 572 4041 9777 10881 11858 572
Size: 3469 5736 1104 977 1139
Fragments arranged by size:
5736 3469 1139 1104 977
AgeI A'CCGG_T
Cuts at: 2414 10813 2414
Size: 8399 4026
AleI CACnn'nnGTG
Cuts at: 2573 3195 3400 3692 2573
Size: 622 205 292 11306
Fragments arranged by size:
11306 622 292 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5148 7342 7760 5148
Size: 2194 418 9813
Fragments arranged by size:
9813 2194 418
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8968 9000 8968
Size: 32 12393
AlwNI CAG_nnn'CTG
Cuts at: 159 370 599 761 3501 3763 3942 4152 4218
Size: 211 229 162 2740 262 179 210 66
Cuts at: 4218 4290 4326 4485 4506 4791 5854 6852 8817
Size: 72 36 159 21 285 1063 998 1965
Cuts at: 8817 8937 9012 9132 9201 9348 9730 11297 159
Size: 120 75 120 69 147 382 1567 1287
Fragments arranged by size:
2740 1965 1567 1287 1063 998 382 285
262 229 211 210 179 162 159 147
120 120 75 72 69 66 36 21
ApaI G_GGCC'C
Cuts at: 431 527 812 1198 5031 5196 5744 6604 6748
Size: 96 285 386 3833 165 548 860 144
Cuts at: 6748 6797 6827 9272 431
Size: 49 30 2445 3584
Fragments arranged by size:
3833 3584 2445 860 548 386 285 165
144 96 49 30
ApaLI G'TGCA_C
Cuts at: 11195 11195
Size: 12425
ApoI r'AATT_y
Cuts at: 7960 8638 9429 11778 12034 7960
Size: 678 791 2349 256 8351
Fragments arranged by size:
8351 2349 791 678 256
AscI GG'CGCG_CC
Cuts at: 221 221
Size: 12425
AseI AT'TA_AT
Cuts at: 20 20
Size: 12425
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12425
AvaI C'yCGr_G
Cuts at: 169 656 779 849 884 932 1016 1597 2133
Size: 487 123 70 35 48 84 581 536
Cuts at: 2133 5178 5189 5363 6035 6597 6904 7262 7305
Size: 3045 11 174 672 562 307 358 43
Cuts at: 7305 7514 8165 8294 9445 169
Size: 209 651 129 1151 3149
Fragments arranged by size:
3149 3045 1151 672 651 581 562 536
487 358 307 209 174 129 123 84
70 48 43 35 11
AvrII C'CTAG_G
Cuts at: 2065 2065
Size: 12425
BamHI G'GATC_C
Cuts at: 1772 4879 5330 9450 1772
Size: 3107 451 4120 4747
Fragments arranged by size:
4747 4120 3107 451
BanI G'GyrC_C
Cuts at: 449 982 1056 1174 1315 1607 2074 2235 2251
Size: 533 74 118 141 292 467 161 16
Cuts at: 2251 3186 3360 3388 3549 4087 4198 4424 5355
Size: 935 174 28 161 538 111 226 931
Cuts at: 5355 5731 5874 6700 8017 8450 9441 9855 10103
Size: 376 143 826 1317 433 991 414 248
Cuts at: 10103 10138 449
Size: 35 2736
Fragments arranged by size:
2736 1317 991 935 931 826 538 533
467 433 414 376 292 248 226 174
161 161 143 141 118 111 74 35
28 16
BanII G_rGCy'C
Cuts at: 431 527 697 812 883 930 1023 1198 1245
Size: 96 170 115 71 47 93 175 47
Cuts at: 1245 1598 2024 2047 3029 3887 4301 4964 5031
Size: 353 426 23 982 858 414 663 67
Cuts at: 5031 5196 5706 5744 6604 6748 6797 6827 9158
Size: 165 510 38 860 144 49 30 2331
Cuts at: 9158 9272 9439 10469 431
Size: 114 167 1030 2387
Fragments arranged by size:
2387 2331 1030 982 860 858 663 510
426 414 353 175 170 167 165 144
115 114 96 93 71 67 49 47
47 38 30 23
BbeI G_GCGC'C
Cuts at: 453 986 1178 2078 2239 5359 5735 8454 9859
Size: 533 192 900 161 3120 376 2719 1405
Cuts at: 9859 10107 453
Size: 248 2771
Fragments arranged by size:
3120 2771 2719 1405 900 533 376 248
192 161
BbsI GAAGACnn'nnnn_
Cuts at: 2291 2602 2748 4178 4624 6182 7235 9357 2291
Size: 311 146 1430 446 1558 1053 2122 5359
Fragments arranged by size:
5359 2122 1558 1430 1053 446 311 146
BbvCI CC'TCA_GC
Cuts at: 2473 3560 4829 5680 6069 6443 7624 8083 8227
Size: 1087 1269 851 389 374 1181 459 144
Cuts at: 8227 8311 8899 2473
Size: 84 588 5999
Fragments arranged by size:
5999 1269 1181 1087 851 588 459 389
374 144 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1137 1171 2250 2284 3444 3478 6016 6050 8493
Size: 34 1079 34 1160 34 2538 34 2443
Cuts at: 8493 8527 1137
Size: 34 5035
Fragments arranged by size:
5035 2538 2443 1160 1079 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3631 7153 10323 11084 12425 3631
Size: 3522 3170 761 1341 3631
Fragments arranged by size:
3631 3522 3170 1341 761
BclI T'GATC_A
Cuts at: 1722 5438 9945 1722
Size: 3716 4507 4202
Fragments arranged by size:
4507 4202 3716
BfrBI ATG'CAT
Cuts at: 2549 2549
Size: 12425
BglI GCCn_nnn'nGGC
Cuts at: 174 647 1012 1126 1208 1631 2503 2747 3061
Size: 473 365 114 82 423 872 244 314
Cuts at: 3061 4135 5021 5035 5182 5191 5636 6046 6543
Size: 1074 886 14 147 9 445 410 497
Cuts at: 6543 6599 6630 7460 7531 7683 8881 174
Size: 56 31 830 71 152 1198 3718
Fragments arranged by size:
3718 1198 1074 886 872 830 497 473
445 423 410 365 314 244 152 147
114 82 71 56 31 14 9
BglII A'GATC_T
Cuts at: 5651 9940 11977 5651
Size: 4289 2037 6099
Fragments arranged by size:
6099 4289 2037
BlpI GC'TnA_GC
Cuts at: 1635 2212 2849 4598 5047 5505 6449 9247 9297
Size: 577 637 1749 449 458 944 2798 50
Cuts at: 9297 9537 1635
Size: 240 4523
Fragments arranged by size:
4523 2798 1749 944 637 577 458 449
240 50
Bme1580I G_kGCm'C
Cuts at: 431 527 812 1198 1880 2582 3040 4090 4121
Size: 96 285 386 682 702 458 1050 31
Cuts at: 4121 4839 5031 5196 5744 5848 6604 6617 6705
Size: 718 192 165 548 104 756 13 88
Cuts at: 6705 6748 6797 6827 9272 10050 10143 11199 431
Size: 43 49 30 2445 778 93 1056 1657
Fragments arranged by size:
2445 1657 1056 1050 778 756 718 702
682 548 458 386 285 192 165 104
96 93 88 49 43 31 30 13
BmgBI CAC'GTC
Cuts at: 575 4035 7326 8730 575
Size: 3460 3291 1404 4270
Fragments arranged by size:
4270 3460 3291 1404
BmrI ACTGGGnnnn_n'
Cuts at: 950 1696 2543 3882 7281 8766 9637 9718 10053
Size: 746 847 1339 3399 1485 871 81 335
Cuts at: 10053 12197 950
Size: 2144 1178
Fragments arranged by size:
3399 2144 1485 1339 1178 871 847 746
335 81
BmtI G_CTAG'C
Cuts at: 8944 8944
Size: 12425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 936 1998 2931 5298 10792 936
Size: 1062 933 2367 5494 2569
Fragments arranged by size:
5494 2569 2367 1062 933
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 475 751 885 1724 1968 2215 2227 2251
Size: 294 276 134 839 244 247 12 24
Cuts at: 2251 2659 2808 3223 3463 3474 3655 3835 3964
Size: 408 149 415 240 11 181 180 129
Cuts at: 3964 4096 4312 4474 4719 4906 5233 5371 5373
Size: 132 216 162 245 187 327 138 2
Cuts at: 5373 5552 6022 7492 7693 8322 8410 9277 181
Size: 179 470 1470 201 629 88 867 3329
Fragments arranged by size:
3329 1470 867 839 629 470 415 408
327 294 276 247 245 244 240 216
201 187 181 180 179 162 149 138
134 132 129 88 24 12 11 2
Bpu10I CC'TnA_GC
Cuts at: 2046 2473 2589 2629 3560 4207 4829 5680 6069
Size: 427 116 40 931 647 622 851 389
Cuts at: 6069 6443 7624 8083 8227 8311 8899 2046
Size: 374 1181 459 144 84 588 5572
Fragments arranged by size:
5572 1181 931 851 647 622 588 459
427 389 374 144 116 84 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 2329 2836 5074 7351 9601 10480 10972 11270
Size: 1131 507 2238 2277 2250 879 492 298
Cuts at: 11270 1198
Size: 2353
Fragments arranged by size:
2353 2277 2250 2238 1131 879 507 492
298
BsaI GGTCTCn'nnnn_
Cuts at: 1999 2101 2123 2812 3255 6151 6975 8755 12129
Size: 102 22 689 443 2896 824 1780 3374
Cuts at: 12129 1999
Size: 2295
Fragments arranged by size:
3374 2896 2295 1780 824 689 443 102
22
BsaAI yAC'GTr
Cuts at: 8949 9417 9778 8949
Size: 468 361 11596
Fragments arranged by size:
11596 468 361
BsaBI GATnn'nnATC
Cuts at: 6 9939 11976 6
Size: 9933 2037 455
Fragments arranged by size:
9933 2037 455
BsaHI Gr'CG_yC
Cuts at: 360 450 550 983 1175 1792 2075 2236 3235
Size: 90 100 433 192 617 283 161 999
Cuts at: 3235 3293 3629 4640 5356 5732 8451 9856 10104
Size: 58 336 1011 716 376 2719 1405 248
Cuts at: 10104 10806 12302 360
Size: 702 1496 483
Fragments arranged by size:
2719 1496 1405 1011 999 716 702 617
483 433 376 336 283 248 192 161
100 90 58
BsaWI w'CCGG_w
Cuts at: 472 603 865 1356 2169 2414 2928 7145 7711
Size: 131 262 491 813 245 514 4217 566
Cuts at: 7711 7979 9136 9614 10135 10813 11087 11234 11645
Size: 268 1157 478 521 678 274 147 411
Cuts at: 11645 472
Size: 1252
Fragments arranged by size:
4217 1252 1157 813 678 566 521 514
491 478 411 274 268 262 245 147
131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 187 211 217 2783 2813 3436 3466 3508
Size: 6 24 6 2566 30 623 30 42
Cuts at: 3508 3526 3538 3556 7114 7144 8046 8076 8581
Size: 18 12 18 3558 30 902 30 505
Cuts at: 8581 8611 8968 8998 9131 9161 9175 9205 181
Size: 30 357 30 133 30 14 30 3401
Fragments arranged by size:
3558 3401 2566 902 623 505 357 133
42 30 30 30 30 30 30 30
30 24 18 18 14 12 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 472 491 920 1144 2553 3058 3096 3099 3354
Size: 19 429 224 1409 505 38 3 255
Cuts at: 3354 3529 3532 3535 4309 5287 5486 6181 6322
Size: 175 3 3 774 978 199 695 141
Cuts at: 6322 6325 6328 6331 6334 6337 6340 6343 6346
Size: 3 3 3 3 3 3 3 3
Cuts at: 6346 6349 6358 6371 6374 6755 7090 7612 7794
Size: 3 9 13 3 381 335 522 182
Cuts at: 7794 8071 8605 9274 472
Size: 277 534 669 3623
Fragments arranged by size:
3623 1409 978 774 695 669 534 522
505 429 381 335 277 255 224 199
182 175 141 38 19 13 9 3
3 3 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 152 226 370 486 858 1116 1644 2033 3087
Size: 74 144 116 372 258 528 389 1054
Cuts at: 3087 3149 3703 3963 4218 4478 4575 4648 4693
Size: 62 554 260 255 260 97 73 45
Cuts at: 4693 4707 4808 4982 5024 5148 5207 5323 5466
Size: 14 101 174 42 124 59 116 143
Cuts at: 5466 5590 5699 5896 5966 6553 6687 6730 6977
Size: 124 109 197 70 587 134 43 247
Cuts at: 6977 7185 7440 7998 8389 9187 9403 9850 10608
Size: 208 255 558 391 798 216 447 758
Cuts at: 10608 11185 11681 152
Size: 577 496 896
Fragments arranged by size:
1054 896 798 758 587 577 558 554
528 496 447 391 389 372 260 260
258 255 255 247 216 208 197 174
144 143 134 124 124 116 116 109
101 97 74 73 70 62 59 45
43 42 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1883 1911 1934 4042 4321 5368 5801 6640 7348
Size: 28 23 2108 279 1047 433 839 708
Cuts at: 7348 7489 7756 8068 8274 8605 8653 8722 8755
Size: 141 267 312 206 331 48 69 33
Cuts at: 8755 1883
Size: 5553
Fragments arranged by size:
5553 2108 1047 839 708 433 331 312
279 267 206 141 69 48 33 28
23
BsiEI CG_ry'CG
Cuts at: 104 249 281 441 495 1294 1760 2224 5288
Size: 145 32 160 54 799 466 464 3064
Cuts at: 5288 8281 9967 10013 11221 104
Size: 2993 1686 46 1208 1308
Fragments arranged by size:
3064 2993 1686 1308 1208 799 466 464
160 145 54 46 32
BsiHKAI G_wGCw'C
Cuts at: 2563 4301 4964 8423 9439 10217 10407 11199 2563
Size: 1738 663 3459 1016 778 190 792 3789
Fragments arranged by size:
3789 3459 1738 1016 792 778 663 190
BsmI GAATG_Cn'
Cuts at: 828 3057 5534 828
Size: 2229 2477 7719
Fragments arranged by size:
7719 2477 2229
BsmBI CGTCTCn'nnnn_
Cuts at: 2267 3226 9009 2267
Size: 959 5783 5683
Fragments arranged by size:
5783 5683 959
Bsp1286I G_dGCh'C
Cuts at: 431 527 697 812 883 930 1023 1198 1245
Size: 96 170 115 71 47 93 175 47
Cuts at: 1245 1598 1880 2024 2047 2563 2582 3029 3040
Size: 353 282 144 23 516 19 447 11
Cuts at: 3040 3887 4090 4121 4301 4839 4964 5031 5196
Size: 847 203 31 180 538 125 67 165
Cuts at: 5196 5706 5744 5848 6604 6617 6705 6748 6797
Size: 510 38 104 756 13 88 43 49
Cuts at: 6797 6827 8423 9158 9272 9439 10050 10143 10217
Size: 30 1596 735 114 167 611 93 74
Cuts at: 10217 10407 10469 11199 431
Size: 190 62 730 1657
Fragments arranged by size:
1657 1596 847 756 735 730 611 538
516 510 447 353 282 203 190 180
175 170 167 165 144 125 115 114
104 96 93 93 88 74 71 67
62 49 47 47 43 38 31 30
23 19 13 11
BspEI T'CCGG_A
Cuts at: 472 603 7145 7711 7979 9136 11645 472
Size: 131 6542 566 268 1157 2509 1252
Fragments arranged by size:
6542 2509 1252 1157 566 268 131
BspHI T'CATG_A
Cuts at: 11601 11601
Size: 12425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1044 1677 4343 4363 7190 8250 8534 8945 8961
Size: 633 2666 20 2827 1060 284 411 16
Cuts at: 8961 9251 9475 9689 9991 10372 1044
Size: 290 224 214 302 381 3097
Fragments arranged by size:
3097 2827 2666 1060 633 411 381 302
290 284 224 214 20 16
BsrBI CCG'CTC
Cuts at: 50 635 1102 1221 1679 1979 2853 2904 2910
Size: 585 467 119 458 300 874 51 6
Cuts at: 2910 7164 7261 7750 10717 10771 12255 12420 50
Size: 4254 97 489 2967 54 1484 165 55
Fragments arranged by size:
4254 2967 1484 874 585 489 467 458
300 165 119 97 55 54 51 6
BsrDI GCAATG_nn'
Cuts at: 5846 9212 10337 5846
Size: 3366 1125 7934
Fragments arranged by size:
7934 3366 1125
BsrFI r'CCGG_y
Cuts at: 177 273 945 2414 3329 7018 7659 7740 8453
Size: 96 672 1469 915 3689 641 81 713
Cuts at: 8453 8600 8744 10423 10604 10813 177
Size: 147 144 1679 181 209 1789
Fragments arranged by size:
3689 1789 1679 1469 915 713 672 641
209 181 147 144 96 81
BssHII G'CGCG_C
Cuts at: 221 221
Size: 12425
BssSI C'ACGA_G
Cuts at: 1429 1483 1537 1859 2056 10696 11054 1429
Size: 54 54 322 197 8640 358 2800
Fragments arranged by size:
8640 2800 358 322 197 54 54
BstAPI GCAn_nnn'nTGC
Cuts at: 3098 4506 5145 6012 7004 7500 8937 9802 3098
Size: 1408 639 867 992 496 1437 865 5721
Fragments arranged by size:
5721 1437 1408 992 867 865 639 496
BstBI TT'CG_AA
Cuts at: 110 10786 110
Size: 10676 1749
BstEII G'GTnAC_C
Cuts at: 3694 7371 8149 3694
Size: 3677 778 7970
Fragments arranged by size:
7970 3677 778
BstXI CCAn_nnnn'nTGG
Cuts at: 2575 4470 4816 5121 8011 8127 9150 2575
Size: 1895 346 305 2890 116 1023 5850
Fragments arranged by size:
5850 2890 1895 1023 346 305 116
BstYI r'GATC_y
Cuts at: 1 1743 1772 3158 4589 4879 5330 5651 7112
Size: 1742 29 1386 1431 290 451 321 1461
Cuts at: 7112 8184 9450 9917 9940 10275 10521 11533 11619
Size: 1072 1266 467 23 335 246 1012 86
Cuts at: 11619 11631 11977 1
Size: 12 346 449
Fragments arranged by size:
1742 1461 1431 1386 1266 1072 1012 467
451 449 346 335 321 290 246 86
29 23 12
Bsu36I CC'TnA_GG
Cuts at: 183 2152 2438 4676 6151 6649 7093 8624 8925
Size: 1969 286 2238 1475 498 444 1531 301
Cuts at: 8925 183
Size: 3683
Fragments arranged by size:
3683 2238 1969 1531 1475 498 444 301
286
BtgI C'CryG_G
Cuts at: 125 249 441 1190 1324 1401 1455 1835 2142
Size: 124 192 749 134 77 54 380 307
Cuts at: 2142 3266 3653 3690 4082 5762 5867 6480 6953
Size: 1124 387 37 392 1680 105 613 473
Cuts at: 6953 7063 7350 7635 125
Size: 110 287 285 4915
Fragments arranged by size:
4915 1680 1124 749 613 473 392 387
380 307 287 285 192 134 124 110
105 77 54 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7569 10375 10556 10719 7569
Size: 2806 181 163 9275
Fragments arranged by size:
9275 2806 181 163
BtsI GCAGTG_nn'
Cuts at: 332 1995 2568 2580 3016 4101 4942 5062 5083
Size: 1663 573 12 436 1085 841 120 21
Cuts at: 5083 5380 5548 6374 6892 8286 8767 9387 9668
Size: 297 168 826 518 1394 481 620 281
Cuts at: 9668 332
Size: 3089
Fragments arranged by size:
3089 1663 1394 1085 841 826 620 573
518 481 436 297 281 168 120 21
12
DraI TTT'AAA
Cuts at: 3791 9424 3791
Size: 5633 6792
DraIII CAC_nnn'GTG
Cuts at: 4664 6124 7882 8473 9398 4664
Size: 1460 1758 591 925 7691
Fragments arranged by size:
7691 1758 1460 925 591
DrdI GACnn_nn'nnGTC
Cuts at: 3642 6967 10131 10989 3642
Size: 3325 3164 858 5078
Fragments arranged by size:
5078 3325 3164 858
EaeI y'GGCC_r
Cuts at: 206 260 278 318 438 492 947 1291 1674
Size: 54 18 40 120 54 455 344 383
Cuts at: 1674 2221 2706 2748 3071 3243 3299 3837 4136
Size: 547 485 42 323 172 56 538 299
Cuts at: 4136 4604 4942 5222 5285 5918 6272 6470 6483
Size: 468 338 280 63 633 354 198 13
Cuts at: 6483 6544 6680 6843 7256 7664 8278 8882 10010
Size: 61 136 163 413 408 614 604 1128
Cuts at: 10010 10184 10575 10602 11947 206
Size: 174 391 27 1345 684
Fragments arranged by size:
1345 1128 684 633 614 604 547 538
485 468 455 413 408 391 383 354
344 338 323 299 280 198 174 172
163 136 120 63 61 56 54 54
42 40 27 18 13
EagI C'GGCC_G
Cuts at: 278 438 492 1291 2221 5285 8278 10010 278
Size: 160 54 799 930 3064 2993 1732 2693
Fragments arranged by size:
3064 2993 2693 1732 930 799 160 54
EarI CTCTTCn'nnn_
Cuts at: 476 2344 3328 3490 3670 7480 8653 8659 9091
Size: 1868 984 162 180 3810 1173 6 432
Cuts at: 9091 9217 10448 10658 476
Size: 126 1231 210 2243
Fragments arranged by size:
3810 2243 1868 1231 1173 984 432 210
180 162 126 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2011 4639 7182 7381 8472 10633 10943 11089 12235
Size: 2628 2543 199 1091 2161 310 146 1146
Cuts at: 12235 2011
Size: 2201
Fragments arranged by size:
2628 2543 2201 2161 1146 1091 310 199
146
EcoICRI GAG'CTC
Cuts at: 4299 4962 9437 4299
Size: 663 4475 7287
Fragments arranged by size:
7287 4475 663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 475 751 885 1724 1832 1968 2215 2227
Size: 294 276 134 839 108 136 247 12
Cuts at: 2227 2251 2659 2808 3031 3223 3463 3474 3655
Size: 24 408 149 223 192 240 11 181
Cuts at: 3655 3835 3964 4096 4312 4474 4636 4719 4906
Size: 180 129 132 216 162 162 83 187
Cuts at: 4906 5233 5250 5371 5373 5552 6022 6194 7059
Size: 327 17 121 2 179 470 172 865
Cuts at: 7059 7426 7492 7504 7693 8224 8322 8410 8815
Size: 367 66 12 189 531 98 88 405
Cuts at: 8815 9241 9277 10250 10682 11429 11697 181
Size: 426 36 973 432 747 268 909
Fragments arranged by size:
973 909 865 839 747 531 470 432
426 408 405 367 327 294 276 268
247 240 223 216 192 189 187 181
180 179 172 162 162 149 136 134
132 129 121 108 98 88 83 66
36 24 17 12 12 11 2
EcoNI CCTnn'n_nnAGG
Cuts at: 457 1862 2119 2641 2950 3301 3871 4210 4267
Size: 1405 257 522 309 351 570 339 57
Cuts at: 4267 4896 7045 8902 457
Size: 629 2149 1857 3980
Fragments arranged by size:
3980 2149 1857 1405 629 570 522 351
339 309 257 57
EcoO109I rG'GnC_Cy
Cuts at: 523 524 808 809 905 1113 1163 1195 1689
Size: 1 284 1 96 208 50 32 494
Cuts at: 1689 2050 2062 2147 2202 2295 2386 2469 2541
Size: 361 12 85 55 93 91 83 72
Cuts at: 2541 3423 3939 3948 4149 4260 4323 4547 5028
Size: 882 516 9 201 111 63 224 481
Cuts at: 5028 5109 5153 5454 5463 5541 5560 6182 6601
Size: 81 44 301 9 78 19 622 419
Cuts at: 6601 6744 6745 6777 6794 6823 6824 6909 6917
Size: 143 1 32 17 29 1 85 8
Cuts at: 6917 7381 7641 7841 7985 8386 8934 9129 9268
Size: 464 260 200 144 401 548 195 139
Cuts at: 9268 9269 9564 523
Size: 1 295 3384
Fragments arranged by size:
3384 882 622 548 516 494 481 464
419 401 361 301 295 284 260 224
208 201 200 195 144 143 139 111
96 93 91 85 85 83 81 78
72 63 55 50 44 32 32 29
19 17 12 9 9 8 1 1
1 1 1
EcoRI G'AATT_C
Cuts at: 7960 8638 9429 7960
Size: 678 791 10956
Fragments arranged by size:
10956 791 678
EcoRV GAT'ATC
Cuts at: 5669 5669
Size: 12425
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 10444 10444
Size: 12425
FseI GG_CCGG'CC
Cuts at: 181 949 181
Size: 768 11657
FspI TGC'GCA
Cuts at: 6520 7594 10206 6520
Size: 1074 2612 8739
Fragments arranged by size:
8739 2612 1074
HaeII r_GCGC'y
Cuts at: 151 159 453 730 986 1178 2078 2239 3477
Size: 8 294 277 256 192 900 161 1238
Cuts at: 3477 4317 4584 5054 5131 5251 5359 5735 5956
Size: 840 267 470 77 120 108 376 221
Cuts at: 5956 6889 7537 7854 8454 8855 9859 10107 11129
Size: 933 648 317 600 401 1004 248 1022
Cuts at: 11129 12108 151
Size: 979 468
Fragments arranged by size:
1238 1022 1004 979 933 900 840 648
600 470 468 401 376 317 294 277
267 256 248 221 192 161 120 108
77 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 187 213 219 679 711 832 864 1728
Size: 6 26 6 460 32 121 32 864
Cuts at: 1728 1760 3106 3138 3526 3558 3669 3701 3874
Size: 32 1346 32 388 32 111 32 173
Cuts at: 3874 3906 4377 4409 5281 5313 6037 6069 6118
Size: 32 471 32 872 32 724 32 49
Cuts at: 6118 6150 7114 7146 7828 7860 9131 9163 9175
Size: 32 964 32 682 32 1271 32 12
Cuts at: 9175 9207 10267 10297 10299 10329 181
Size: 32 1060 30 2 30 2277
Fragments arranged by size:
2277 1346 1271 1060 964 872 864 724
682 471 460 388 173 121 111 49
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
30 26 12 6 6 2
HincII GTy'rAC
Cuts at: 4332 8342 9288 9464 4332
Size: 4010 946 176 7293
Fragments arranged by size:
7293 4010 946 176
HindIII A'AGCT_T
Cuts at: 4015 6641 4015
Size: 2626 9799
KasI G'GCGC_C
Cuts at: 449 982 1174 2074 2235 5355 5731 8450 9855
Size: 533 192 900 161 3120 376 2719 1405
Cuts at: 9855 10103 449
Size: 248 2771
Fragments arranged by size:
3120 2771 2719 1405 900 533 376 248
192 161
KpnI G_GTAC'C
Cuts at: 1319 3190 4428 9445 1319
Size: 1871 1238 5017 4299
Fragments arranged by size:
5017 4299 1871 1238
MluI A'CGCG_T
Cuts at: 11858 11858
Size: 12425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 269 869 1017 1982 7832 11096 11280 269
Size: 600 148 965 5850 3264 184 1414
Fragments arranged by size:
5850 3264 1414 965 600 184 148
MscI TGG'CCA
Cuts at: 2708 3245 3301 3839 4138 4944 6485 6682 6845
Size: 537 56 538 299 806 1541 197 163
Cuts at: 6845 7666 10186 2708
Size: 821 2520 4947
Fragments arranged by size:
4947 2520 1541 821 806 538 537 299
197 163 56
MslI CAynn'nnrTG
Cuts at: 2573 3195 3400 3692 5827 5872 6580 7252 9178
Size: 622 205 292 2135 45 708 672 1926
Cuts at: 9178 10414 10541 2573
Size: 1236 127 4457
Fragments arranged by size:
4457 2135 1926 1236 708 672 622 292
205 127 45
MspA1I CmG'CkG
Cuts at: 251 443 858 978 1063 1125 1403 1457 3087
Size: 192 415 120 85 62 278 54 1630
Cuts at: 3087 3810 3855 4287 4455 4812 4974 5172 5211
Size: 723 45 432 168 357 162 198 39
Cuts at: 5211 5319 5916 6021 6469 6757 7332 7404 7497
Size: 108 597 105 448 288 575 72 93
Cuts at: 7497 7803 8673 8961 9078 9234 9301 9536 9850
Size: 306 870 288 117 156 67 235 314
Cuts at: 9850 10210 11223 11468 251
Size: 360 1013 245 1208
Fragments arranged by size:
1630 1208 1013 870 723 597 575 448
432 415 360 357 314 306 288 288
278 245 235 198 192 168 162 156
120 117 108 105 93 85 72 67
62 54 45 39
NaeI GCC'GGC
Cuts at: 179 947 7742 8455 8602 8746 10606 179
Size: 768 6795 713 147 144 1860 1998
Fragments arranged by size:
6795 1998 1860 768 713 147 144
NarI GG'CG_CC
Cuts at: 450 983 1175 2075 2236 5356 5732 8451 9856
Size: 533 192 900 161 3120 376 2719 1405
Cuts at: 9856 10104 450
Size: 248 2771
Fragments arranged by size:
3120 2771 2719 1405 900 533 376 248
192 161
NcoI C'CATG_G
Cuts at: 125 3690 5762 5867 6480 125
Size: 3565 2072 105 613 6070
Fragments arranged by size:
6070 3565 2072 613 105
NgoMIV G'CCGG_C
Cuts at: 177 945 7740 8453 8600 8744 10604 177
Size: 768 6795 713 147 144 1860 1998
Fragments arranged by size:
6795 1998 1860 768 713 147 144
NheI G'CTAG_C
Cuts at: 8940 8940
Size: 12425
NotI GC'GGCC_GC
Cuts at: 438 492 1291 438
Size: 54 799 11572
Fragments arranged by size:
11572 799 54
NruI TCG'CGA
Cuts at: 1159 3255 1159
Size: 2096 10329
NsiI A_TGCA'T
Cuts at: 2551 2551
Size: 12425
NspI r_CATG'y
Cuts at: 4045 4401 6585 9126 9478 9978 10885 4045
Size: 356 2184 2541 352 500 907 5585
Fragments arranged by size:
5585 2541 2184 907 500 356 352
PasI CC'CwG_GG
Cuts at: 364 431 813 1245 4152 5114 6291 6913 364
Size: 67 382 432 2907 962 1177 622 5876
Fragments arranged by size:
5876 2907 1177 962 622 432 382 67
PciI A'CATG_T
Cuts at: 4041 10881 4041
Size: 6840 5585
PflMI CCAn_nnn'nTGG
Cuts at: 159 370 1191 3132 3858 4152 4218 4326 4977
Size: 211 821 1941 726 294 66 108 651
Cuts at: 4977 6231 8400 159
Size: 1254 2169 4184
Fragments arranged by size:
4184 2169 1941 1254 821 726 651 294
211 108 66
PfoI T'CCnGG_A
Cuts at: 901 3623 3872 4301 5642 8069 901
Size: 2722 249 429 1341 2427 5257
Fragments arranged by size:
5257 2722 2427 1341 429 249
PmeI GTTT'AAAC
Cuts at: 9424 9424
Size: 12425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8968 9000 11564 11596 8968
Size: 32 2564 32 9797
Fragments arranged by size:
9797 2564 32 32
PpuMI rG'GwC_Cy
Cuts at: 905 1113 1689 2062 2147 2202 2295 2541 3948
Size: 208 576 373 85 55 93 246 1407
Cuts at: 3948 4260 5454 5463 5560 6909 6917 7641 7985
Size: 312 1194 9 97 1349 8 724 344
Cuts at: 7985 8934 905
Size: 949 4396
Fragments arranged by size:
4396 1407 1349 1194 949 724 576 373
344 312 246 208 97 93 85 55
9 8
PshAI GACnn'nnGTC
Cuts at: 851 1445 851
Size: 594 11831
PspOMI G'GGCC_C
Cuts at: 427 523 808 1194 5027 5192 5740 6600 6744
Size: 96 285 386 3833 165 548 860 144
Cuts at: 6744 6793 6823 9268 427
Size: 49 30 2445 3584
Fragments arranged by size:
3833 3584 2445 860 548 386 285 165
144 96 49 30
PspXI vC'TCGA_Gb
Cuts at: 849 8294 849
Size: 7445 4980
PstI C_TGCA'G
Cuts at: 339 358 2120 2575 2924 2968 3023 3086 4271
Size: 19 1762 455 349 44 55 63 1185
Cuts at: 4271 4511 5339 7618 8378 8690 8894 9233 9472
Size: 240 828 2279 760 312 204 339 239
Cuts at: 9472 10157 339
Size: 685 2607
Fragments arranged by size:
2607 2279 1762 1185 828 760 685 455
349 339 312 240 239 204 63 55
44 19
PvuI CG_AT'CG
Cuts at: 104 104
Size: 12425
PvuII CAG'CTG
Cuts at: 3087 3810 3855 4455 4812 4974 5172 5211 5319
Size: 723 45 600 357 162 198 39 108
Cuts at: 5319 5916 6757 7332 7404 7497 7803 8673 8961
Size: 597 841 575 72 93 306 870 288
Cuts at: 8961 9078 9301 9850 10210 3087
Size: 117 223 549 360 5302
Fragments arranged by size:
5302 870 841 723 600 597 575 549
360 357 306 288 223 198 162 117
108 93 72 45 39
RsrII CG'GwC_CG
Cuts at: 10620 10620
Size: 12425
SacI G_AGCT'C
Cuts at: 4301 4964 9439 4301
Size: 663 4475 7287
Fragments arranged by size:
7287 4475 663
SacII CC_GC'GG
Cuts at: 252 444 1404 1458 252
Size: 192 960 54 11219
Fragments arranged by size:
11219 960 192 54
SalI G'TCGA_C
Cuts at: 4330 8340 9462 4330
Size: 4010 1122 7293
Fragments arranged by size:
7293 4010 1122
SanDI GG'GwC_CC
Cuts at: 2147 5463 6917 2147
Size: 3316 1454 7655
Fragments arranged by size:
7655 3316 1454
SapI GCTCTTCn'nnn_
Cuts at: 476 3490 3670 7480 8659 9217 10448 10658 476
Size: 3014 180 3810 1179 558 1231 210 2243
Fragments arranged by size:
3810 3014 2243 1231 1179 558 210 180
SbfI CC_TGCA'GG
Cuts at: 2924 2968 9472 2924
Size: 44 6504 5877
Fragments arranged by size:
6504 5877 44
ScaI AGT'ACT
Cuts at: 11942 11942
Size: 12425
SexAI A'CCwGG_T
Cuts at: 1031 3773 4919 1031
Size: 2742 1146 8537
Fragments arranged by size:
8537 2742 1146
SfcI C'TryA_G
Cuts at: 33 335 354 2116 2571 2770 2920 2964 3019
Size: 302 19 1762 455 199 150 44 55
Cuts at: 3019 3082 3478 4267 4507 5074 5335 6784 7614
Size: 63 396 789 240 567 261 1449 830
Cuts at: 7614 8114 8374 8686 8805 8890 9229 9468 10153
Size: 500 260 312 119 85 339 239 685
Cuts at: 10153 11146 11337 33
Size: 993 191 1121
Fragments arranged by size:
1762 1449 1121 993 830 789 685 567
500 455 396 339 312 302 261 260
240 239 199 191 150 119 85 63
55 44 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 174 1012 4135 5182 5191 174
Size: 838 3123 1047 9 7408
Fragments arranged by size:
7408 3123 1047 838 9
SfoI GGC'GCC
Cuts at: 451 984 1176 2076 2237 5357 5733 8452 9857
Size: 533 192 900 161 3120 376 2719 1405
Cuts at: 9857 10105 451
Size: 248 2771
Fragments arranged by size:
3120 2771 2719 1405 900 533 376 248
192 161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12425
SgrAI Cr'CCGG_yG
Cuts at: 8453 8453
Size: 12425
SmaI CCC'GGG
Cuts at: 171 658 781 1018 1599 2135 5365 6599 7516
Size: 487 123 237 581 536 3230 1234 917
Cuts at: 7516 8167 9447 171
Size: 651 1280 3149
Fragments arranged by size:
3230 3149 1280 1234 917 651 581 536
487 237 123
SmlI C'TyrA_G
Cuts at: 849 1213 2344 2815 5089 7366 7561 8294 9580
Size: 364 1131 471 2274 2277 195 733 1286
Cuts at: 9580 10495 10987 11249 849
Size: 915 492 262 2025
Fragments arranged by size:
2277 2274 2025 1286 1131 915 733 492
471 364 262 195
SnaBI TAC'GTA
Cuts at: 9417 9417
Size: 12425
SpeI A'CTAG_T
Cuts at: 67 67
Size: 12425
SphI G_CATG'C
Cuts at: 4401 9126 9478 9978 4401
Size: 4725 352 500 6848
Fragments arranged by size:
6848 4725 500 352
SrfI GCCC'GGGC
Cuts at: 1018 1599 6599 7516 1018
Size: 581 5000 917 5927
Fragments arranged by size:
5927 5000 917 581
StuI AGG'CCT
Cuts at: 1006 2962 4025 4129 5348 6061 6633 7993 1006
Size: 1956 1063 104 1219 713 572 1360 5438
Fragments arranged by size:
5438 1956 1360 1219 1063 713 572 104
StyI C'CwwG_G
Cuts at: 125 950 2065 2102 2255 3302 3690 4245 4256
Size: 825 1115 37 153 1047 388 555 11
Cuts at: 4256 4550 5156 5762 5867 6275 6458 6473 6480
Size: 294 606 606 105 408 183 15 7
Cuts at: 6480 6486 6498 6665 6773 7377 7769 7864 8057
Size: 6 12 167 108 604 392 95 193
Cuts at: 8057 8855 9559 9913 125
Size: 798 704 354 2637
Fragments arranged by size:
2637 1115 1047 825 798 704 606 606
604 555 408 392 388 354 294 193
183 167 153 108 105 95 37 15
12 11 7 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3537 5315 5835 6991 7432 8137 8815 10008 10141
Size: 1778 520 1156 441 705 678 1193 133
Cuts at: 10141 10600 10809 3537
Size: 459 209 5153
Fragments arranged by size:
5153 1778 1193 1156 705 678 520 459
441 209 133
TatI w'GTAC_w
Cuts at: 8051 9203 11940 8051
Size: 1152 2737 8536
Fragments arranged by size:
8536 2737 1152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1031 1063 2426 2458 2783 2815 3436 3468 3508
Size: 32 1363 32 325 32 621 32 40
Cuts at: 3508 3540 5054 5086 8046 8078 8581 8613 11942
Size: 32 1514 32 2960 32 503 32 3329
Cuts at: 11942 11974 1031
Size: 32 1482
Fragments arranged by size:
3329 2960 1514 1482 1363 621 503 325
40 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 7234 10222 7234
Size: 2988 9437
XbaI T'CTAG_A
Cuts at: 9456 9456
Size: 12425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 310 3084 3294 3354 3688 4470 5865 7533 8397
Size: 2774 210 60 334 782 1395 1668 864
Cuts at: 8397 9920 310
Size: 1523 2815
Fragments arranged by size:
2815 2774 1668 1523 1395 864 782 334
210 60
XhoI C'TCGA_G
Cuts at: 849 8294 849
Size: 7445 4980
XmaI C'CCGG_G
Cuts at: 169 656 779 1016 1597 2133 5363 6597 7514
Size: 487 123 237 581 536 3230 1234 917
Cuts at: 7514 8165 9445 169
Size: 651 1280 3149
Fragments arranged by size:
3230 3149 1280 1234 917 651 581 536
487 237 123
XmnI GAAnn'nnTTC
Cuts at: 6220 7036 8485 6220
Size: 816 1449 10160
Fragments arranged by size:
10160 1449 816
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AhdI BaeI BsiWI BsrGI BstZ17I ClaI CspCI
FspAI HpaI MfeI NdeI PacI PmlI PsiI PsrI
SspI SwaI ZraI