Restriction map for
pF1KSDA1785
(Circular) (Six-base) MAPSORT of: pF1KSDA1785.seq Check: 3035 from: 1 to: 4988
pF1KSDA1785 4988 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 893 2026 893
Size: 1133 3855
Acc65I G'GTAC_C
Cuts at: 1622 2004 1622
Size: 382 4606
AclI AA'CG_TT
Cuts at: 4828 4828
Size: 4988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1557 2813 3245 3992 4260 433
Size: 1124 1256 432 747 268 1161
Fragments arranged by size:
1256 1161 1124 747 432 268
AflII C'TTAA_G
Cuts at: 1543 1543
Size: 4988
AflIII A'CryG_T
Cuts at: 1609 2340 3444 4421 1609
Size: 731 1104 977 2176
Fragments arranged by size:
2176 1104 977 731
AgeI A'CCGG_T
Cuts at: 3376 3376
Size: 4988
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 335 707 1745 1907 335
Size: 372 1038 162 3416
Fragments arranged by size:
3416 1038 372 162
AlwNI CAG_nnn'CTG
Cuts at: 700 1177 2293 3860 700
Size: 477 1116 1567 1828
Fragments arranged by size:
1828 1567 1116 477
ApaLI G'TGCA_C
Cuts at: 1778 3758 1778
Size: 1980 3008
ApoI r'AATT_y
Cuts at: 298 1182 1636 1851 1992 4341 4597 298
Size: 884 454 215 141 2349 256 689
Fragments arranged by size:
2349 884 689 454 256 215 141
AseI AT'TA_AT
Cuts at: 20 860 1096 20
Size: 840 236 3912
Fragments arranged by size:
3912 840 236
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
AvaI C'yCGr_G
Cuts at: 158 2008 158
Size: 1850 3138
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1325 1358 1325
Size: 33 4955
BamHI G'GATC_C
Cuts at: 1242 2013 1242
Size: 771 4217
BanI G'GyrC_C
Cuts at: 281 1622 2004 2418 2666 2701 281
Size: 1341 382 414 248 35 2568
Fragments arranged by size:
2568 1341 414 382 248 35
BanII G_rGCy'C
Cuts at: 343 378 2002 3032 343
Size: 35 1624 1030 2299
Fragments arranged by size:
2299 1624 1030 35
BbeI G_GCGC'C
Cuts at: 2422 2670 2422
Size: 248 4740
BciVI GTATCCnnnnn_n'
Cuts at: 2886 3647 4988 2886
Size: 761 1341 2886
Fragments arranged by size:
2886 1341 761
BclI T'GATC_A
Cuts at: 2508 2508
Size: 4988
BfrBI ATG'CAT
Cuts at: 1196 1196
Size: 4988
BglII A'GATC_T
Cuts at: 906 2503 4540 906
Size: 1597 2037 1354
Fragments arranged by size:
2037 1597 1354
BlpI GC'TnA_GC
Cuts at: 2100 2100
Size: 4988
Bme1580I G_kGCm'C
Cuts at: 284 1782 2613 2706 3762 284
Size: 1498 831 93 1056 1510
Fragments arranged by size:
1510 1498 1056 831 93
BmrI ACTGGGnnnn_n'
Cuts at: 2200 2281 2616 4760 2200
Size: 81 335 2144 2428
Fragments arranged by size:
2428 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 237 3355 237
Size: 3118 1870
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2164 3043 3535 3833 2164
Size: 879 492 298 3319
Fragments arranged by size:
3319 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4692 4692
Size: 4988
BsaAI yAC'GTr
Cuts at: 578 1980 2341 578
Size: 1402 361 3225
Fragments arranged by size:
3225 1402 361
BsaBI GATnn'nnATC
Cuts at: 6 2502 4539 6
Size: 2496 2037 455
Fragments arranged by size:
2496 2037 455
BsaHI Gr'CG_yC
Cuts at: 2419 2667 3369 4865 2419
Size: 248 702 1496 2542
Fragments arranged by size:
2542 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2177 2698 3376 3650 3797 4208 2177
Size: 521 678 274 147 411 2957
Fragments arranged by size:
2957 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 434 464 434
Size: 30 4958
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 470 226
Size: 244 4744
BseYI C'CCAG_C
Cuts at: 1329 2413 3171 3748 4244 1329
Size: 1084 758 577 496 2073
Fragments arranged by size:
2073 1084 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 1058 713
Size: 345 4643
BsiEI CG_ry'CG
Cuts at: 104 2530 2576 3784 104
Size: 2426 46 1208 1308
Fragments arranged by size:
2426 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1782 2002 2780 2970 3762 1782
Size: 220 778 190 792 3008
Fragments arranged by size:
3008 792 778 220 190
BsmI GAATG_Cn'
Cuts at: 1088 1088
Size: 4988
Bsp1286I G_dGCh'C
Cuts at: 284 343 378 1782 2002 2613 2706 2780 2970
Size: 59 35 1404 220 611 93 74 190
Cuts at: 2970 3032 3762 284
Size: 62 730 1510
Fragments arranged by size:
1510 1404 730 611 220 190 93 74
62 59 35
BspEI T'CCGG_A
Cuts at: 4208 4208
Size: 4988
BspHI T'CATG_A
Cuts at: 716 1754 4164 716
Size: 1038 2410 1540
Fragments arranged by size:
2410 1540 1038
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2038 2252 2554 2935 2038
Size: 214 302 381 4091
Fragments arranged by size:
4091 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3280 3334 4818 4983 50
Size: 3230 54 1484 165 55
Fragments arranged by size:
3230 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 319 679 947 1879 2900 319
Size: 360 268 932 1021 2407
Fragments arranged by size:
2407 1021 932 360 268
BsrFI r'CCGG_y
Cuts at: 2986 3167 3376 2986
Size: 181 209 4598
Fragments arranged by size:
4598 209 181
BssSI C'ACGA_G
Cuts at: 1898 3259 3617 1898
Size: 1361 358 3269
Fragments arranged by size:
3269 1361 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1370 2365 1370
Size: 995 3993
BstBI TT'CG_AA
Cuts at: 110 3349 110
Size: 3239 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 192 192
Size: 4988
BstYI r'GATC_y
Cuts at: 1 129 906 1242 2013 2480 2503 2838 3084
Size: 128 777 336 771 467 23 335 246
Cuts at: 3084 4096 4182 4194 4540 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 777 771 467 449 346 336 335
246 128 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4988
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2938 3119 3282 2938
Size: 181 163 4644
Fragments arranged by size:
4644 181 163
BtsI GCAGTG_nn'
Cuts at: 324 1891 2231 324
Size: 1567 340 3081
Fragments arranged by size:
3081 1567 340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 794 829 794
Size: 35 4953
DraI TTT'AAA
Cuts at: 1987 1987
Size: 4988
DrdI GACnn_nn'nnGTC
Cuts at: 2694 3552 2694
Size: 858 4130
EaeI y'GGCC_r
Cuts at: 266 504 2573 2747 3138 3165 4510 266
Size: 238 2069 174 391 27 1345 744
Fragments arranged by size:
2069 1345 744 391 238 174 27
EagI C'GGCC_G
Cuts at: 2573 2573
Size: 4988
EarI CTCTTCn'nnn_
Cuts at: 488 1037 1586 3011 3221 488
Size: 549 549 1425 210 2255
Fragments arranged by size:
2255 1425 549 549 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3196 3506 3652 4798 3196
Size: 310 146 1146 3386
Fragments arranged by size:
3386 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2000 2000
Size: 4988
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1557 2813 3245 3992 4260 433
Size: 1124 1256 432 747 268 1161
Fragments arranged by size:
1256 1161 1124 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1446 2127 1446
Size: 681 4307
EcoRI G'AATT_C
Cuts at: 298 1992 298
Size: 1694 3294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1587 3007 1587
Size: 1420 3568
FspI TGC'GCA
Cuts at: 2769 2769
Size: 4988
HaeII r_GCGC'y
Cuts at: 1163 2422 2670 3692 4671 1163
Size: 1259 248 1022 979 1480
Fragments arranged by size:
1480 1259 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 434 466 1148 1180 2830 2860 2862 2892 434
Size: 32 682 32 1650 30 2 30 2530
Fragments arranged by size:
2530 1650 682 32 32 30 30 2
HincII GTy'rAC
Cuts at: 453 2027 453
Size: 1574 3414
KasI G'GCGC_C
Cuts at: 2418 2666 2418
Size: 248 4740
KpnI G_GTAC'C
Cuts at: 1626 2008 1626
Size: 382 4606
MluI A'CGCG_T
Cuts at: 4421 4421
Size: 4988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3659 3843 3659
Size: 184 4804
MscI TGG'CCA
Cuts at: 506 2749 506
Size: 2243 2745
MslI CAynn'nnrTG
Cuts at: 689 721 1787 1884 2977 3104 689
Size: 32 1066 97 1093 127 2573
Fragments arranged by size:
2573 1093 1066 127 97 32
MspA1I CmG'CkG
Cuts at: 1423 2099 2413 2773 3786 4031 1423
Size: 676 314 360 1013 245 2380
Fragments arranged by size:
2380 1013 676 360 314 245
NaeI GCC'GGC
Cuts at: 3169 3169
Size: 4988
NarI GG'CG_CC
Cuts at: 2419 2667 2419
Size: 248 4740
NcoI C'CATG_G
Cuts at: 125 125
Size: 4988
NgoMIV G'CCGG_C
Cuts at: 3167 3167
Size: 4988
NsiI A_TGCA'T
Cuts at: 1198 1198
Size: 4988
NspI r_CATG'y
Cuts at: 1613 2041 2541 3448 1613
Size: 428 500 907 3153
Fragments arranged by size:
3153 907 500 428
PciI A'CATG_T
Cuts at: 1609 3444 1609
Size: 1835 3153
PmeI GTTT'AAAC
Cuts at: 1987 1987
Size: 4988
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 4956
PshAI GACnn'nnGTC
Cuts at: 1712 1712
Size: 4988
PsiI TTA'TAA
Cuts at: 1928 1928
Size: 4988
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 937 969 937
Size: 32 4956
PstI C_TGCA'G
Cuts at: 402 2035 2720 402
Size: 1633 685 2670
Fragments arranged by size:
2670 1633 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4988
PvuII CAG'CTG
Cuts at: 1423 2413 2773 1423
Size: 990 360 3638
Fragments arranged by size:
3638 990 360
RsrII CG'GwC_CG
Cuts at: 3183 3183
Size: 4988
SacI G_AGCT'C
Cuts at: 2002 2002
Size: 4988
SalI G'TCGA_C
Cuts at: 2025 2025
Size: 4988
SapI GCTCTTCn'nnn_
Cuts at: 1037 3011 3221 1037
Size: 1974 210 2804
Fragments arranged by size:
2804 1974 210
SbfI CC_TGCA'GG
Cuts at: 2035 2035
Size: 4988
ScaI AGT'ACT
Cuts at: 4505 4505
Size: 4988
SfcI C'TryA_G
Cuts at: 33 398 1306 2031 2716 3709 3900 33
Size: 365 908 725 685 993 191 1121
Fragments arranged by size:
1121 993 908 725 685 365 191
SfoI GGC'GCC
Cuts at: 2420 2668 2420
Size: 248 4740
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4988
SmaI CCC'GGG
Cuts at: 2010 2010
Size: 4988
SmlI C'TyrA_G
Cuts at: 1543 2143 3058 3550 3812 1543
Size: 600 915 492 262 2719
Fragments arranged by size:
2719 915 600 492 262
SnaBI TAC'GTA
Cuts at: 1980 1980
Size: 4988
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4988
SphI G_CATG'C
Cuts at: 2041 2541 2041
Size: 500 4488
SspI AAT'ATT
Cuts at: 810 972 810
Size: 162 4826
StuI AGG'CCT
Cuts at: 1325 1325
Size: 4988
StyI C'CwwG_G
Cuts at: 125 1022 1555 2122 2476 125
Size: 897 533 567 354 2637
Fragments arranged by size:
2637 897 567 533 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2571 2704 3163 3372 2571
Size: 133 459 209 4187
Fragments arranged by size:
4187 459 209 133
TatI w'GTAC_w
Cuts at: 1495 4503 1495
Size: 3008 1980
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4505 4537 4505
Size: 32 4956
Tth111I GACn'n_nGTC
Cuts at: 2785 2785
Size: 4988
XbaI T'CTAG_A
Cuts at: 867 1500 2019 867
Size: 633 519 3836
Fragments arranged by size:
3836 633 519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2483 2483
Size: 4988
XmaI C'CCGG_G
Cuts at: 2008 2008
Size: 4988
XmnI GAAnn'nnTTC
Cuts at: 1109 1570 1109
Size: 461 4527
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BglI BmgBI BmtI BpmI
Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI XhoI ZraI