Restriction map for
pF1KSDA1809
(Circular) (Six-base) MAPSORT of: pF1KSDA1809.seq Check: 6520 from: 1 to: 5456
pF1KSDA1809 5456 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 1763 1763
Size: 5456
AccI GT'mk_AC
Cuts at: 2494 2494
Size: 5456
Acc65I G'GTAC_C
Cuts at: 2472 2472
Size: 5456
AclI AA'CG_TT
Cuts at: 5296 5296
Size: 5456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 752 1405 1782 3281 3713 4460 4728 752
Size: 653 377 1499 432 747 268 1480
Fragments arranged by size:
1499 1480 747 653 432 377 268
AflII C'TTAA_G
Cuts at: 159 2437 159
Size: 2278 3178
AflIII A'CryG_T
Cuts at: 637 1100 1361 2087 2808 3912 4889 637
Size: 463 261 726 721 1104 977 1204
Fragments arranged by size:
1204 1104 977 726 721 463 261
AgeI A'CCGG_T
Cuts at: 3844 3844
Size: 5456
AleI CACnn'nnGTG
Cuts at: 1007 1007
Size: 5456
AlwNI CAG_nnn'CTG
Cuts at: 350 1821 2761 4328 350
Size: 1471 940 1567 1478
Fragments arranged by size:
1567 1478 1471 940
ApaI G_GGCC'C
Cuts at: 2314 2314
Size: 5456
ApaLI G'TGCA_C
Cuts at: 1306 1365 4226 1306
Size: 59 2861 2536
Fragments arranged by size:
2861 2536 59
ApoI r'AATT_y
Cuts at: 172 482 1285 2460 4809 5065 172
Size: 310 803 1175 2349 256 563
Fragments arranged by size:
2349 1175 803 563 310 256
AscI GG'CGCG_CC
Cuts at: 1690 1690
Size: 5456
AseI AT'TA_AT
Cuts at: 20 20
Size: 5456
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
AvaI C'yCGr_G
Cuts at: 181 1005 1950 1965 2038 2233 2256 2313 2427
Size: 824 945 15 73 195 23 57 114
Cuts at: 2427 2476 181
Size: 49 3161
Fragments arranged by size:
3161 945 824 195 114 73 57 49
23 15
BamHI G'GATC_C
Cuts at: 2481 2481
Size: 5456
BanI G'GyrC_C
Cuts at: 1477 2472 2886 3134 3169 1477
Size: 995 414 248 35 3764
Fragments arranged by size:
3764 995 414 248 35
BanII G_rGCy'C
Cuts at: 240 564 626 2017 2314 2397 2436 2470 3500
Size: 324 62 1391 297 83 39 34 1030
Cuts at: 3500 240
Size: 2196
Fragments arranged by size:
2196 1391 1030 324 297 83 62 39
34
BbeI G_GCGC'C
Cuts at: 2890 3138 2890
Size: 248 5208
BbsI GAAGACnn'nnnn_
Cuts at: 1405 1892 1405
Size: 487 4969
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2019 2053 2019
Size: 34 5422
BciVI GTATCCnnnnn_n'
Cuts at: 772 3354 4115 5456 772
Size: 2582 761 1341 772
Fragments arranged by size:
2582 1341 772 761
BclI T'GATC_A
Cuts at: 2976 2976
Size: 5456
BglI GCCn_nnn'nGGC
Cuts at: 1325 1325
Size: 5456
BglII A'GATC_T
Cuts at: 2971 5008 2971
Size: 2037 3419
BlpI GC'TnA_GC
Cuts at: 1675 1780 2011 2568 1675
Size: 105 231 557 4563
Fragments arranged by size:
4563 557 231 105
Bme1580I G_kGCm'C
Cuts at: 1310 1369 2314 3081 3174 4230 1310
Size: 59 945 767 93 1056 2536
Fragments arranged by size:
2536 1056 945 767 93 59
BmgBI CAC'GTC
Cuts at: 1103 1103
Size: 5456
BmrI ACTGGGnnnn_n'
Cuts at: 2668 2749 3084 5228 2668
Size: 81 335 2144 2896
Fragments arranged by size:
2896 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1725 1980 2214 3823 1725
Size: 255 234 1609 3358
Fragments arranged by size:
3358 1609 255 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 546 1702 2106 2145 224
Size: 322 1156 404 39 3535
Fragments arranged by size:
3535 1156 404 322 39
Bpu10I CC'TnA_GC
Cuts at: 2146 2146
Size: 5456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1498 2381 2632 3511 4003 4301 1498
Size: 883 251 879 492 298 2653
Fragments arranged by size:
2653 883 879 492 298 251
BsaI GGTCTCn'nnnn_
Cuts at: 1453 5160 1453
Size: 3707 1749
BsaAI yAC'GTr
Cuts at: 2448 2809 2448
Size: 361 5095
BsaBI GATnn'nnATC
Cuts at: 6 864 2970 5007 6
Size: 858 2106 2037 455
Fragments arranged by size:
2106 2037 858 455
BsaHI Gr'CG_yC
Cuts at: 1195 1760 2887 3135 3837 5333 1195
Size: 565 1127 248 702 1496 1318
Fragments arranged by size:
1496 1318 1127 702 565 248
BsaWI w'CCGG_w
Cuts at: 2645 3166 3844 4118 4265 4676 2645
Size: 521 678 274 147 411 3425
Fragments arranged by size:
3425 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1435 1465 2074 2104 2134 1435
Size: 30 609 30 30 4757
Fragments arranged by size:
4757 609 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 528 1199 1217 1594 1603 1630 1639 1642
Size: 278 671 18 377 9 27 9 3
Cuts at: 1642 1645 1648 2172 2187 250
Size: 3 3 524 15 3519
Fragments arranged by size:
3519 671 524 377 278 27 18 15
9 9 3 3 3
BseYI C'CCAG_C
Cuts at: 152 625 794 1132 1235 1992 2002 2323 2881
Size: 473 169 338 103 757 10 321 558
Cuts at: 2881 3639 4216 4712 152
Size: 758 577 496 896
Fragments arranged by size:
896 758 757 577 558 496 473 338
321 169 103 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 536 608 800 505
Size: 31 72 192 5161
Fragments arranged by size:
5161 192 72 31
BsiEI CG_ry'CG
Cuts at: 104 1724 2066 2998 3044 4252 104
Size: 1620 342 932 46 1208 1308
Fragments arranged by size:
1620 1308 1208 932 342 46
BsiHKAI G_wGCw'C
Cuts at: 240 323 564 1310 1369 1781 2195 2290 2397
Size: 83 241 746 59 412 414 95 107
Cuts at: 2397 2470 3248 3438 4230 240
Size: 73 778 190 792 1466
Fragments arranged by size:
1466 792 778 746 414 412 241 190
107 95 83 73 59
Bsp1286I G_dGCh'C
Cuts at: 240 323 564 626 1310 1369 1781 2017 2195
Size: 83 241 62 684 59 412 236 178
Cuts at: 2195 2290 2314 2397 2436 2470 3081 3174 3248
Size: 95 24 83 39 34 611 93 74
Cuts at: 3248 3438 3500 4230 240
Size: 190 62 730 1466
Fragments arranged by size:
1466 730 684 611 412 241 236 190
178 95 93 83 83 74 62 62
59 39 34 24
BspEI T'CCGG_A
Cuts at: 4676 4676
Size: 5456
BspHI T'CATG_A
Cuts at: 252 4632 252
Size: 4380 1076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 851 2063 2506 2720 3022 3403 851
Size: 1212 443 214 302 381 2904
Fragments arranged by size:
2904 1212 443 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3748 3802 5286 5451 50
Size: 3698 54 1484 165 55
Fragments arranged by size:
3698 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 345 3368 345
Size: 3023 2433
BsrFI r'CCGG_y
Cuts at: 1127 1310 1897 2107 3454 3635 3844 1127
Size: 183 587 210 1347 181 209 2739
Fragments arranged by size:
2739 1347 587 210 209 183 181
BsrGI T'GTAC_A
Cuts at: 640 640
Size: 5456
BssHII G'CGCG_C
Cuts at: 1508 1690 1508
Size: 182 5274
BssSI C'ACGA_G
Cuts at: 1699 3727 4085 1699
Size: 2028 358 3070
Fragments arranged by size:
3070 2028 358
BstAPI GCAn_nnn'nTGC
Cuts at: 350 595 1352 2833 350
Size: 245 757 1481 2973
Fragments arranged by size:
2973 1481 757 245
BstBI TT'CG_AA
Cuts at: 110 3817 110
Size: 3707 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 420 1488 2302 420
Size: 1068 814 3574
Fragments arranged by size:
3574 1068 814
BstYI r'GATC_y
Cuts at: 1 2136 2252 2481 2948 2971 3306 3552 4564
Size: 2135 116 229 467 23 335 246 1012
Cuts at: 4564 4650 4662 5008 1
Size: 86 12 346 449
Fragments arranged by size:
2135 1012 467 449 346 335 246 229
116 86 23 12
BtgI C'CryG_G
Cuts at: 222 419 751 807 1061 1520 1938 222
Size: 197 332 56 254 459 418 3740
Fragments arranged by size:
3740 459 418 332 254 197 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2083 3406 3587 3750 2083
Size: 1323 181 163 3789
Fragments arranged by size:
3789 1323 181 163
BtsI GCAGTG_nn'
Cuts at: 280 2104 2699 280
Size: 1824 595 3037
Fragments arranged by size:
3037 1824 595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2386 2421 2386
Size: 35 5421
DraI TTT'AAA
Cuts at: 2455 2455
Size: 5456
DrdI GACnn_nn'nnGTC
Cuts at: 1434 3162 4020 1434
Size: 1728 858 2870
Fragments arranged by size:
2870 1728 858
EaeI y'GGCC_r
Cuts at: 992 1526 1842 1850 1895 1935 2063 3041 3215
Size: 534 316 8 45 40 128 978 174
Cuts at: 3215 3606 3633 4978 992
Size: 391 27 1345 1470
Fragments arranged by size:
1470 1345 978 534 391 316 174 128
45 40 27 8
EagI C'GGCC_G
Cuts at: 2063 3041 2063
Size: 978 4478
EarI CTCTTCn'nnn_
Cuts at: 974 1580 1598 1607 1616 3479 3689 974
Size: 606 18 9 9 1863 210 2741
Fragments arranged by size:
2741 1863 606 210 18 9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 991 1209 3664 3974 4120 5266 991
Size: 218 2455 310 146 1146 1181
Fragments arranged by size:
2455 1181 1146 310 218 146
EcoICRI GAG'CTC
Cuts at: 238 562 2395 2468 238
Size: 324 1833 73 3226
Fragments arranged by size:
3226 1833 324 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 546 752 1405 1702 1782 2106 2145 3281
Size: 322 206 653 297 80 324 39 1136
Cuts at: 3281 3713 4460 4728 224
Size: 432 747 268 952
Fragments arranged by size:
1136 952 747 653 432 324 322 297
268 206 80 39
EcoNI CCTnn'n_nnAGG
Cuts at: 1017 1852 1017
Size: 835 4621
EcoO109I rG'GnC_Cy
Cuts at: 531 1818 1971 2595 531
Size: 1287 153 624 3392
Fragments arranged by size:
3392 1287 624 153
EcoRI G'AATT_C
Cuts at: 172 2460 172
Size: 2288 3168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 217 1200 3475 217
Size: 983 2275 2198
Fragments arranged by size:
2275 2198 983
FseI GG_CCGG'CC
Cuts at: 1131 1131
Size: 5456
FspI TGC'GCA
Cuts at: 3237 3237
Size: 5456
HaeII r_GCGC'y
Cuts at: 900 969 1392 1502 2890 3138 4160 5139 900
Size: 69 423 110 1388 248 1022 979 1217
Fragments arranged by size:
1388 1217 1022 979 423 248 110 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 551 583 698 730 1435 1467 1873
Size: 32 71 32 115 32 705 32 406
Cuts at: 1873 1905 2410 2442 3298 3328 3330 3360 448
Size: 32 505 32 856 30 2 30 2544
Fragments arranged by size:
2544 856 705 505 406 115 71 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2495 2495
Size: 5456
KasI G'GCGC_C
Cuts at: 2886 3134 2886
Size: 248 5208
KpnI G_GTAC'C
Cuts at: 2476 2476
Size: 5456
MluI A'CGCG_T
Cuts at: 1361 4889 1361
Size: 3528 1928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1886 2168 4127 4311 1463
Size: 423 282 1959 184 2608
Fragments arranged by size:
2608 1959 423 282 184
MscI TGG'CCA
Cuts at: 994 1528 3217 994
Size: 534 1689 3233
Fragments arranged by size:
3233 1689 534
MslI CAynn'nnrTG
Cuts at: 1007 2300 3445 3572 1007
Size: 1293 1145 127 2891
Fragments arranged by size:
2891 1293 1145 127
MspA1I CmG'CkG
Cuts at: 753 1116 1940 2267 2567 2881 3241 4254 4499
Size: 363 824 327 300 314 360 1013 245
Cuts at: 4499 753
Size: 1710
Fragments arranged by size:
1710 1013 824 363 360 327 314 300
245
NaeI GCC'GGC
Cuts at: 1129 1899 2109 3637 1129
Size: 770 210 1528 2948
Fragments arranged by size:
2948 1528 770 210
NarI GG'CG_CC
Cuts at: 2887 3135 2887
Size: 248 5208
NcoI C'CATG_G
Cuts at: 807 1061 807
Size: 254 5202
NgoMIV G'CCGG_C
Cuts at: 1127 1897 2107 3635 1127
Size: 770 210 1528 2948
Fragments arranged by size:
2948 1528 770 210
NspI r_CATG'y
Cuts at: 641 1410 2509 3009 3916 641
Size: 769 1099 500 907 2181
Fragments arranged by size:
2181 1099 907 769 500
PciI A'CATG_T
Cuts at: 637 3912 637
Size: 3275 2181
PflMI CCAn_nnn'nTGG
Cuts at: 671 1821 671
Size: 1150 4306
PfoI T'CCnGG_A
Cuts at: 1562 1562
Size: 5456
PmeI GTTT'AAAC
Cuts at: 2455 2455
Size: 5456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 2032 2064 2077 2109 4595 4627 114
Size: 32 1886 32 13 32 2486 32 943
Fragments arranged by size:
2486 1886 943 32 32 32 32 13
PpuMI rG'GwC_Cy
Cuts at: 1818 1818
Size: 5456
PshAI GACnn'nnGTC
Cuts at: 1445 1445
Size: 5456
PspOMI G'GGCC_C
Cuts at: 2310 2310
Size: 5456
PstI C_TGCA'G
Cuts at: 2503 3188 2503
Size: 685 4771
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5456
PvuII CAG'CTG
Cuts at: 2881 3241 2881
Size: 360 5096
RsrII CG'GwC_CG
Cuts at: 1908 3651 1908
Size: 1743 3713
SacI G_AGCT'C
Cuts at: 240 564 2397 2470 240
Size: 324 1833 73 3226
Fragments arranged by size:
3226 1833 324 73
SacII CC_GC'GG
Cuts at: 754 1941 754
Size: 1187 4269
SalI G'TCGA_C
Cuts at: 2493 2493
Size: 5456
SapI GCTCTTCn'nnn_
Cuts at: 3479 3689 3479
Size: 210 5246
SbfI CC_TGCA'GG
Cuts at: 2503 2503
Size: 5456
ScaI AGT'ACT
Cuts at: 4973 4973
Size: 5456
SexAI A'CCwGG_T
Cuts at: 1298 1814 1298
Size: 516 4940
SfcI C'TryA_G
Cuts at: 33 2499 3184 4177 4368 33
Size: 2466 685 993 191 1121
Fragments arranged by size:
2466 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2888 3136 2888
Size: 248 5208
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
SgrAI Cr'CCGG_yG
Cuts at: 1310 1310
Size: 5456
SmaI CCC'GGG
Cuts at: 1952 2235 2315 2478 1952
Size: 283 80 163 4930
Fragments arranged by size:
4930 283 163 80
SmlI C'TyrA_G
Cuts at: 159 1513 2038 2396 2427 2437 2611 3526 4018
Size: 1354 525 358 31 10 174 915 492
Cuts at: 4018 4280 159
Size: 262 1335
Fragments arranged by size:
1354 1335 915 525 492 358 262 174
31 10
SnaBI TAC'GTA
Cuts at: 2448 2448
Size: 5456
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5456
SphI G_CATG'C
Cuts at: 2509 3009 2509
Size: 500 4956
SrfI GCCC'GGGC
Cuts at: 2315 2315
Size: 5456
StuI AGG'CCT
Cuts at: 1807 1807
Size: 5456
StyI C'CwwG_G
Cuts at: 807 1061 2590 2944 807
Size: 254 1529 354 3319
Fragments arranged by size:
3319 1529 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 921 1012 1384 3039 3172 3631 3840 921
Size: 91 372 1655 133 459 209 2537
Fragments arranged by size:
2537 1655 459 372 209 133 91
TatI w'GTAC_w
Cuts at: 640 2090 4971 640
Size: 1450 2881 1125
Fragments arranged by size:
2881 1450 1125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1402 1434 4973 5005 1402
Size: 32 3539 32 1853
Fragments arranged by size:
3539 1853 32 32
Tth111I GACn'n_nGTC
Cuts at: 3253 3253
Size: 5456
XbaI T'CTAG_A
Cuts at: 475 2487 475
Size: 2012 3444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1488 2951 1488
Size: 1463 3993
XhoI C'TCGA_G
Cuts at: 2038 2427 2038
Size: 389 5067
XmaI C'CCGG_G
Cuts at: 1950 2233 2313 2476 1950
Size: 283 80 163 4930
Fragments arranged by size:
4930 283 163 80
XmnI GAAnn'nnTTC
Cuts at: 197 2047 197
Size: 1850 3606
ZraI GAC'GTC
Cuts at: 1761 1761
Size: 5456
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AlfI AloI AvrII BaeI BbvCI
BfrBI BmtI BsiWI BsmI BsmBI BstEII BstZ17I Bsu36I
ClaI DraIII EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PsiI
PspXI PsrI SanDI SfiI SspI SwaI