Restriction map for
pF1KSDA1810
(Circular) (Six-base) MAPSORT of: pF1KSDA1810.seq Check: 8617 from: 1 to: 4832
pF1KSDA1810 4832 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 964 964
Size: 4832
AccI GT'mk_AC
Cuts at: 1870 1870
Size: 4832
Acc65I G'GTAC_C
Cuts at: 1848 1848
Size: 4832
AclI AA'CG_TT
Cuts at: 363 4672 363
Size: 4309 523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 1568 2657 3089 3836 4104 405
Size: 1163 1089 432 747 268 1133
Fragments arranged by size:
1163 1133 1089 747 432 268
AflIII A'CryG_T
Cuts at: 2184 3288 4265 2184
Size: 1104 977 2751
Fragments arranged by size:
2751 1104 977
AgeI A'CCGG_T
Cuts at: 3220 3220
Size: 4832
AleI CACnn'nnGTG
Cuts at: 139 223 139
Size: 84 4748
AlwNI CAG_nnn'CTG
Cuts at: 1246 1282 1294 1312 1467 1488 1696 1720 1800
Size: 36 12 18 155 21 208 24 80
Cuts at: 1800 2137 3704 1246
Size: 337 1567 2374
Fragments arranged by size:
2374 1567 337 208 155 80 36 24
21 18 12
ApaI G_GGCC'C
Cuts at: 1141 1568 1141
Size: 427 4405
ApaLI G'TGCA_C
Cuts at: 682 3602 682
Size: 2920 1912
ApoI r'AATT_y
Cuts at: 1836 4185 4441 1836
Size: 2349 256 2227
Fragments arranged by size:
2349 2227 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4832
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4832
AvaI C'yCGr_G
Cuts at: 1852 1852
Size: 4832
BamHI G'GATC_C
Cuts at: 1857 1857
Size: 4832
BanI G'GyrC_C
Cuts at: 874 1249 1784 1848 2262 2510 2545 874
Size: 375 535 64 414 248 35 3161
Fragments arranged by size:
3161 535 414 375 248 64 35
BanII G_rGCy'C
Cuts at: 824 1141 1443 1568 1763 1778 1846 2876 824
Size: 317 302 125 195 15 68 1030 2780
Fragments arranged by size:
2780 1030 317 302 195 125 68 15
BbeI G_GCGC'C
Cuts at: 1788 2266 2514 1788
Size: 478 248 4106
Fragments arranged by size:
4106 478 248
BbsI GAAGACnn'nnnn_
Cuts at: 279 279
Size: 4832
BbvCI CC'TCA_GC
Cuts at: 485 1064 485
Size: 579 4253
BciVI GTATCCnnnnn_n'
Cuts at: 809 2730 3491 4832 809
Size: 1921 761 1341 809
Fragments arranged by size:
1921 1341 809 761
BclI T'GATC_A
Cuts at: 674 2352 674
Size: 1678 3154
BglI GCCn_nnn'nGGC
Cuts at: 160 1309 1572 160
Size: 1149 263 3420
Fragments arranged by size:
3420 1149 263
BglII A'GATC_T
Cuts at: 2347 4384 2347
Size: 2037 2795
BlpI GC'TnA_GC
Cuts at: 1944 1944
Size: 4832
Bme1580I G_kGCm'C
Cuts at: 686 1141 1400 1568 1735 2457 2550 3606 686
Size: 455 259 168 167 722 93 1056 1912
Fragments arranged by size:
1912 1056 722 455 259 168 167 93
BmrI ACTGGGnnnn_n'
Cuts at: 1214 2044 2125 2460 4604 1214
Size: 830 81 335 2144 1442
Fragments arranged by size:
2144 1442 830 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 4832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 808 1091 1465 1489 1697 1822 553
Size: 255 283 374 24 208 125 3563
Fragments arranged by size:
3563 374 283 255 208 125 24
Bpu10I CC'TnA_GC
Cuts at: 485 1064 1170 485
Size: 579 106 4147
Fragments arranged by size:
4147 579 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2008 2887 3379 3677 2008
Size: 879 492 298 3163
Fragments arranged by size:
3163 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4536 4536
Size: 4832
BsaAI yAC'GTr
Cuts at: 1824 2185 1824
Size: 361 4471
BsaBI GATnn'nnATC
Cuts at: 6 2346 4383 6
Size: 2340 2037 455
Fragments arranged by size:
2340 2037 455
BsaHI Gr'CG_yC
Cuts at: 1514 1785 2263 2511 3213 4709 1514
Size: 271 478 248 702 1496 1637
Fragments arranged by size:
1637 1496 702 478 271 248
BsaWI w'CCGG_w
Cuts at: 2021 2542 3220 3494 3641 4052 2021
Size: 521 678 274 147 411 2801
Fragments arranged by size:
2801 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1166 1196 1166
Size: 30 4802
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 832 1178 1331 1444 1615 1623 1753 832
Size: 346 153 113 171 8 130 3911
Fragments arranged by size:
3911 346 171 153 130 113 8
BseYI C'CCAG_C
Cuts at: 247 1165 1414 2257 3015 3592 4088 247
Size: 918 249 843 758 577 496 991
Fragments arranged by size:
991 918 843 758 577 496 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 1223 538
Size: 685 4147
BsiEI CG_ry'CG
Cuts at: 104 2374 2420 3628 104
Size: 2270 46 1208 1308
Fragments arranged by size:
2270 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 353 530 686 824 1443 1778 1846 2624 2814
Size: 177 156 138 619 335 68 778 190
Cuts at: 2814 3606 353
Size: 792 1579
Fragments arranged by size:
1579 792 778 619 335 190 177 156
138 68
Bsp1286I G_dGCh'C
Cuts at: 353 530 686 824 1141 1400 1443 1568 1735
Size: 177 156 138 317 259 43 125 167
Cuts at: 1735 1763 1778 1846 2457 2550 2624 2814 2876
Size: 28 15 68 611 93 74 190 62
Cuts at: 2876 3606 353
Size: 730 1579
Fragments arranged by size:
1579 730 611 317 259 190 177 167
156 138 125 93 74 68 62 43
28 15
BspEI T'CCGG_A
Cuts at: 4052 4052
Size: 4832
BspHI T'CATG_A
Cuts at: 662 671 4008 662
Size: 9 3337 1486
Fragments arranged by size:
3337 1486 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 955 964 1882 2096 2398 2779 955
Size: 9 918 214 302 381 3008
Fragments arranged by size:
3008 918 381 302 214 9
BsrBI CCG'CTC
Cuts at: 50 1458 3124 3178 4662 4827 50
Size: 1408 1666 54 1484 165 55
Fragments arranged by size:
1666 1484 1408 165 55 54
BsrDI GCAATG_nn'
Cuts at: 836 2744 836
Size: 1908 2924
BsrFI r'CCGG_y
Cuts at: 1781 2830 3011 3220 1781
Size: 1049 181 209 3393
Fragments arranged by size:
3393 1049 209 181
BsrGI T'GTAC_A
Cuts at: 263 602 650 263
Size: 339 48 4445
Fragments arranged by size:
4445 339 48
BssSI C'ACGA_G
Cuts at: 3103 3461 3103
Size: 358 4474
BstAPI GCAn_nnn'nTGC
Cuts at: 851 1488 2209 851
Size: 637 721 3474
Fragments arranged by size:
3474 721 637
BstBI TT'CG_AA
Cuts at: 110 3193 110
Size: 3083 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 720 720
Size: 4832
BstYI r'GATC_y
Cuts at: 1 1857 2324 2347 2682 2928 3940 4026 4038
Size: 1856 467 23 335 246 1012 86 12
Cuts at: 4038 4384 1
Size: 346 449
Fragments arranged by size:
1856 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1748 1748
Size: 4832
BtgI C'CryG_G
Cuts at: 560 1133 560
Size: 573 4259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 965 2782 2963 3126 965
Size: 1817 181 163 2671
Fragments arranged by size:
2671 1817 181 163
BtsI GCAGTG_nn'
Cuts at: 146 1504 2075 146
Size: 1358 571 2903
Fragments arranged by size:
2903 1358 571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1040 1075 1599 1634 1040
Size: 35 524 35 4238
Fragments arranged by size:
4238 524 35 35
DraI TTT'AAA
Cuts at: 1831 1831
Size: 4832
DraIII CAC_nnn'GTG
Cuts at: 561 561
Size: 4832
DrdI GACnn_nn'nnGTC
Cuts at: 2538 3396 2538
Size: 858 3974
EaeI y'GGCC_r
Cuts at: 152 722 2417 2591 2982 3009 4354 152
Size: 570 1695 174 391 27 1345 630
Fragments arranged by size:
1695 1345 630 570 391 174 27
EagI C'GGCC_G
Cuts at: 2417 2417
Size: 4832
EarI CTCTTCn'nnn_
Cuts at: 448 455 463 854 2855 3065 448
Size: 7 8 391 2001 210 2215
Fragments arranged by size:
2215 2001 391 210 8 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1768 3040 3350 3496 4642 1768
Size: 1272 310 146 1146 1958
Fragments arranged by size:
1958 1272 1146 310 146
EcoICRI GAG'CTC
Cuts at: 822 1441 1776 1844 822
Size: 619 335 68 3810
Fragments arranged by size:
3810 619 335 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 553 808 1091 1465 1489 1568 1697 1822
Size: 148 255 283 374 24 79 129 125
Cuts at: 1822 2657 3089 3836 4104 405
Size: 835 432 747 268 1133
Fragments arranged by size:
1133 835 747 432 374 283 268 255
148 129 125 79 24
EcoO109I rG'GnC_Cy
Cuts at: 1086 1138 1564 1565 1971 1086
Size: 52 426 1 406 3947
Fragments arranged by size:
3947 426 406 52 1
EcoRI G'AATT_C
Cuts at: 1836 1836
Size: 4832
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 336 2851 336
Size: 2515 2317
FspI TGC'GCA
Cuts at: 2613 2613
Size: 4832
HaeII r_GCGC'y
Cuts at: 166 1788 2266 2514 3536 4515 166
Size: 1622 478 248 1022 979 483
Fragments arranged by size:
1622 1022 979 483 478 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2674 2704 2706 2736 2674
Size: 30 2 30 4770
Fragments arranged by size:
4770 30 30 2
HincII GTy'rAC
Cuts at: 1590 1871 1590
Size: 281 4551
KasI G'GCGC_C
Cuts at: 1784 2262 2510 1784
Size: 478 248 4106
Fragments arranged by size:
4106 478 248
KpnI G_GTAC'C
Cuts at: 1852 1852
Size: 4832
MluI A'CGCG_T
Cuts at: 4265 4265
Size: 4832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3503 3687 3503
Size: 184 4648
MscI TGG'CCA
Cuts at: 724 2593 724
Size: 1869 2963
MslI CAynn'nnrTG
Cuts at: 139 223 514 2821 2948 139
Size: 84 291 2307 127 2023
Fragments arranged by size:
2307 2023 291 127 84
MspA1I CmG'CkG
Cuts at: 914 936 1243 1943 2257 2617 3630 3875 914
Size: 22 307 700 314 360 1013 245 1871
Fragments arranged by size:
1871 1013 700 360 314 307 245 22
NaeI GCC'GGC
Cuts at: 1783 3013 1783
Size: 1230 3602
NarI GG'CG_CC
Cuts at: 1785 2263 2511 1785
Size: 478 248 4106
Fragments arranged by size:
4106 478 248
NcoI C'CATG_G
Cuts at: 1133 1133
Size: 4832
NgoMIV G'CCGG_C
Cuts at: 1781 3011 1781
Size: 1230 3602
NspI r_CATG'y
Cuts at: 609 1116 1885 2385 3292 609
Size: 507 769 500 907 2149
Fragments arranged by size:
2149 907 769 507 500
PasI CC'CwG_GG
Cuts at: 584 1083 584
Size: 499 4333
PciI A'CATG_T
Cuts at: 3288 3288
Size: 4832
PflMI CCAn_nnn'nTGG
Cuts at: 480 831 1294 1312 480
Size: 351 463 18 4000
Fragments arranged by size:
4000 463 351 18
PmeI GTTT'AAAC
Cuts at: 1831 1831
Size: 4832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 3971
Size: 32 4800
PspOMI G'GGCC_C
Cuts at: 1137 1564 1137
Size: 427 4405
PstI C_TGCA'G
Cuts at: 1484 1529 1796 1879 2564 1484
Size: 45 267 83 685 3752
Fragments arranged by size:
3752 685 267 83 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4832
PvuII CAG'CTG
Cuts at: 914 1243 2257 2617 914
Size: 329 1014 360 3129
Fragments arranged by size:
3129 1014 360 329
RsrII CG'GwC_CG
Cuts at: 3027 3027
Size: 4832
SacI G_AGCT'C
Cuts at: 824 1443 1778 1846 824
Size: 619 335 68 3810
Fragments arranged by size:
3810 619 335 68
SalI G'TCGA_C
Cuts at: 1869 1869
Size: 4832
SapI GCTCTTCn'nnn_
Cuts at: 2855 3065 2855
Size: 210 4622
SbfI CC_TGCA'GG
Cuts at: 1879 1879
Size: 4832
ScaI AGT'ACT
Cuts at: 4349 4349
Size: 4832
SfcI C'TryA_G
Cuts at: 33 1480 1525 1613 1792 1875 2560 3553 3744
Size: 1447 45 88 179 83 685 993 191
Cuts at: 3744 33
Size: 1121
Fragments arranged by size:
1447 1121 993 685 191 179 88 83
45
SfiI GGCCn_nnn'nGGCC
Cuts at: 1572 1572
Size: 4832
SfoI GGC'GCC
Cuts at: 1786 2264 2512 1786
Size: 478 248 4106
Fragments arranged by size:
4106 478 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4832
SgrAI Cr'CCGG_yG
Cuts at: 1781 1781
Size: 4832
SmaI CCC'GGG
Cuts at: 1854 1854
Size: 4832
SmlI C'TyrA_G
Cuts at: 1987 2902 3394 3656 1987
Size: 915 492 262 3163
Fragments arranged by size:
3163 915 492 262
SnaBI TAC'GTA
Cuts at: 1824 1824
Size: 4832
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4832
SphI G_CATG'C
Cuts at: 1885 2385 1885
Size: 500 4332
StuI AGG'CCT
Cuts at: 274 1710 274
Size: 1436 3396
StyI C'CwwG_G
Cuts at: 1133 1559 1966 2320 1133
Size: 426 407 354 3645
Fragments arranged by size:
3645 426 407 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2415 2548 3007 3216 2415
Size: 133 459 209 4031
Fragments arranged by size:
4031 459 209 133
TatI w'GTAC_w
Cuts at: 263 602 650 4347 263
Size: 339 48 3697 748
Fragments arranged by size:
3697 748 339 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 783 815 1166 1198 4349 4381 783
Size: 32 351 32 3151 32 1234
Fragments arranged by size:
3151 1234 351 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 237 838 2629 237
Size: 601 1791 2440
Fragments arranged by size:
2440 1791 601
XbaI T'CTAG_A
Cuts at: 1863 1863
Size: 4832
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 249 960 2327 249
Size: 711 1367 2754
Fragments arranged by size:
2754 1367 711
XmaI C'CCGG_G
Cuts at: 1852 1852
Size: 4832
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I ClaI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SrfI SspI SwaI XhoI XmnI
ZraI