Restriction map for
pF1KSDA1813
(Circular) (Six-base) MAPSORT of: pF1KSDA1813.seq Check: 5738 from: 1 to: 5144
pF1KSDA1813 5144 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 872 1293 1764 1941 872
Size: 421 471 177 4075
Fragments arranged by size:
4075 471 421 177
AccI GT'mk_AC
Cuts at: 1608 2182 1608
Size: 574 4570
Acc65I G'GTAC_C
Cuts at: 2160 2160
Size: 5144
AclI AA'CG_TT
Cuts at: 4984 4984
Size: 5144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 698 1129 1504 2969 3401 4148 4416 191
Size: 507 431 375 1465 432 747 268 919
Fragments arranged by size:
1465 919 747 507 432 431 375 268
AfeI AGC'GCT
Cuts at: 1054 1054
Size: 5144
AflIII A'CryG_T
Cuts at: 2496 3600 4577 2496
Size: 1104 977 3063
Fragments arranged by size:
3063 1104 977
AgeI A'CCGG_T
Cuts at: 3532 3532
Size: 5144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1733 1733
Size: 5144
AlwNI CAG_nnn'CTG
Cuts at: 144 721 758 1043 1152 1293 1359 1494 1662
Size: 577 37 285 109 141 66 135 168
Cuts at: 1662 2028 2449 4016 144
Size: 366 421 1567 1272
Fragments arranged by size:
1567 1272 577 421 366 285 168 141
135 109 66 37
ApaI G_GGCC'C
Cuts at: 284 682 929 1284 1845 284
Size: 398 247 355 561 3583
Fragments arranged by size:
3583 561 398 355 247
ApaLI G'TGCA_C
Cuts at: 3914 3914
Size: 5144
ApoI r'AATT_y
Cuts at: 2148 4497 4753 2148
Size: 2349 256 2539
Fragments arranged by size:
2539 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5144
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
AvaI C'yCGr_G
Cuts at: 241 455 590 626 908 1019 1420 1622 1628
Size: 214 135 36 282 111 401 202 6
Cuts at: 1628 2057 2164 241
Size: 429 107 3221
Fragments arranged by size:
3221 429 401 282 214 202 135 111
107 36 6
AvrII C'CTAG_G
Cuts at: 361 978 361
Size: 617 4527
BamHI G'GATC_C
Cuts at: 2169 2169
Size: 5144
BanI G'GyrC_C
Cuts at: 829 860 2160 2574 2822 2857 829
Size: 31 1300 414 248 35 3116
Fragments arranged by size:
3116 1300 414 248 35 31
BanII G_rGCy'C
Cuts at: 284 600 682 929 1284 1421 1629 1745 1845
Size: 316 82 247 355 137 208 116 100
Cuts at: 1845 2066 2158 3188 284
Size: 221 92 1030 2240
Fragments arranged by size:
2240 1030 355 316 247 221 208 137
116 100 92 82
BbeI G_GCGC'C
Cuts at: 2578 2826 2578
Size: 248 4896
BbvCI CC'TCA_GC
Cuts at: 1721 1721
Size: 5144
BciVI GTATCCnnnnn_n'
Cuts at: 3042 3803 5144 3042
Size: 761 1341 3042
Fragments arranged by size:
3042 1341 761
BclI T'GATC_A
Cuts at: 2664 2664
Size: 5144
BglI GCCn_nnn'nGGC
Cuts at: 269 1936 2089 269
Size: 1667 153 3324
Fragments arranged by size:
3324 1667 153
BglII A'GATC_T
Cuts at: 1472 2129 2659 4696 1472
Size: 657 530 2037 1920
Fragments arranged by size:
2037 1920 657 530
BlpI GC'TnA_GC
Cuts at: 1685 2038 2256 1685
Size: 353 218 4573
Fragments arranged by size:
4573 353 218
Bme1580I G_kGCm'C
Cuts at: 284 349 359 471 682 863 894 929 1275
Size: 65 10 112 211 181 31 35 346
Cuts at: 1275 1284 1845 2769 2862 3918 284
Size: 9 561 924 93 1056 1510
Fragments arranged by size:
1510 1056 924 561 346 211 181 112
93 65 35 31 10 9
BmrI ACTGGGnnnn_n'
Cuts at: 932 2356 2437 2772 4916 932
Size: 1424 81 335 2144 1160
Fragments arranged by size:
2144 1424 1160 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3511 3511
Size: 5144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 179 547 942 1399 1657 2065 2071 2128
Size: 1 368 395 457 258 408 6 57
Cuts at: 2128 178
Size: 3194
Fragments arranged by size:
3194 457 408 395 368 258 57 6
1
Bpu10I CC'TnA_GC
Cuts at: 1721 1721
Size: 5144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 2320 3199 3691 3989 1453
Size: 867 879 492 298 2608
Fragments arranged by size:
2608 879 867 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1121 1432 4848 1121
Size: 311 3416 1417
Fragments arranged by size:
3416 1417 311
BsaAI yAC'GTr
Cuts at: 2136 2497 2136
Size: 361 4783
BsaBI GATnn'nnATC
Cuts at: 6 550 2658 4695 6
Size: 544 2108 2037 455
Fragments arranged by size:
2108 2037 544 455
BsaHI Gr'CG_yC
Cuts at: 2575 2823 3525 5021 2575
Size: 248 702 1496 2698
Fragments arranged by size:
2698 1496 702 248
BsaWI w'CCGG_w
Cuts at: 410 823 2333 2854 3532 3806 3953 4364 410
Size: 413 1510 521 678 274 147 411 1190
Fragments arranged by size:
1510 1190 678 521 413 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 1024 1054 1429 1459 328
Size: 30 666 30 375 30 4013
Fragments arranged by size:
4013 666 375 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 245 678 681 684 687 696 699 945 1216
Size: 433 3 3 3 9 3 246 271
Cuts at: 1216 1450 1909 2125 245
Size: 234 459 216 3264
Fragments arranged by size:
3264 459 433 271 246 234 216 9
3 3 3 3
BseYI C'CCAG_C
Cuts at: 348 427 746 994 1705 2569 3327 3904 4400
Size: 79 319 248 711 864 758 577 496
Cuts at: 4400 348
Size: 1092
Fragments arranged by size:
1092 864 758 711 577 496 319 248
79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1084 1540 1825 157
Size: 927 456 285 3476
Fragments arranged by size:
3476 927 456 285
BsiEI CG_ry'CG
Cuts at: 104 1730 2686 2732 3940 104
Size: 1626 956 46 1208 1308
Fragments arranged by size:
1626 1308 1208 956 46
BsiHKAI G_wGCw'C
Cuts at: 970 1421 1629 2066 2158 2936 3126 3918 970
Size: 451 208 437 92 778 190 792 2196
Fragments arranged by size:
2196 792 778 451 437 208 190 92
Bsp1286I G_dGCh'C
Cuts at: 284 349 359 471 600 682 863 894 929
Size: 65 10 112 129 82 181 31 35
Cuts at: 929 970 1275 1284 1421 1629 1745 1845 2066
Size: 41 305 9 137 208 116 100 221
Cuts at: 2066 2158 2769 2862 2936 3126 3188 3918 284
Size: 92 611 93 74 190 62 730 1510
Fragments arranged by size:
1510 730 611 305 221 208 190 181
137 129 116 112 100 93 92 82
74 65 62 41 35 31 10 9
BspEI T'CCGG_A
Cuts at: 410 4364 410
Size: 3954 1190
BspHI T'CATG_A
Cuts at: 4320 4320
Size: 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 872 1293 1764 1941 2194 2408 2710 3091 872
Size: 421 471 177 253 214 302 381 2925
Fragments arranged by size:
2925 471 421 381 302 253 214 177
BsrBI CCG'CTC
Cuts at: 50 1206 1383 1404 1881 1923 3436 3490 4974
Size: 1156 177 21 477 42 1513 54 1484
Cuts at: 4974 5139 50
Size: 165 55
Fragments arranged by size:
1513 1484 1156 477 177 165 55 54
42 21
BsrDI GCAATG_nn'
Cuts at: 3056 3056
Size: 5144
BsrFI r'CCGG_y
Cuts at: 230 947 2000 2009 3142 3323 3532 230
Size: 717 1053 9 1133 181 209 1842
Fragments arranged by size:
1842 1133 1053 717 209 181 9
BssSI C'ACGA_G
Cuts at: 3415 3773 3415
Size: 358 4786
BstAPI GCAn_nnn'nTGC
Cuts at: 144 475 2028 2521 144
Size: 331 1553 493 2767
Fragments arranged by size:
2767 1553 493 331
BstBI TT'CG_AA
Cuts at: 110 3505 110
Size: 3395 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 304 880 304
Size: 576 4568
BstYI r'GATC_y
Cuts at: 1 545 1472 2129 2169 2636 2659 2994 3240
Size: 544 927 657 40 467 23 335 246
Cuts at: 3240 4252 4338 4350 4696 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 927 657 544 467 449 346 335
246 86 40 23 12
BtgI C'CryG_G
Cuts at: 125 267 486 873 125
Size: 142 219 387 4396
Fragments arranged by size:
4396 387 219 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3094 3275 3438 3094
Size: 181 163 4800
Fragments arranged by size:
4800 181 163
BtsI GCAGTG_nn'
Cuts at: 179 444 892 1017 2117 2387 179
Size: 265 448 125 1100 270 2936
Fragments arranged by size:
2936 1100 448 270 265 125
DraI TTT'AAA
Cuts at: 2143 2143
Size: 5144
DrdI GACnn_nn'nnGTC
Cuts at: 2850 3708 2850
Size: 858 4286
EaeI y'GGCC_r
Cuts at: 128 786 945 1727 2081 2729 2903 3294 3321
Size: 658 159 782 354 648 174 391 27
Cuts at: 3321 4666 128
Size: 1345 606
Fragments arranged by size:
1345 782 658 648 606 391 354 174
159 27
EagI C'GGCC_G
Cuts at: 1727 2729 1727
Size: 1002 4142
EarI CTCTTCn'nnn_
Cuts at: 705 717 3167 3377 705
Size: 12 2450 210 2472
Fragments arranged by size:
2472 2450 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 283 544 1798 3352 3662 3808 4954 283
Size: 261 1254 1554 310 146 1146 473
Fragments arranged by size:
1554 1254 1146 473 310 261 146
EcoICRI GAG'CTC
Cuts at: 1419 1627 2064 2156 1419
Size: 208 437 92 4407
Fragments arranged by size:
4407 437 208 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 179 191 547 698 942 1129 1399 1504
Size: 1 12 356 151 244 187 270 105
Cuts at: 1504 1657 2065 2071 2128 2969 3401 4148 4416
Size: 153 408 6 57 841 432 747 268
Cuts at: 4416 178
Size: 906
Fragments arranged by size:
906 841 747 432 408 356 270 268
244 187 153 151 105 57 12 6
1
EcoNI CCTnn'n_nnAGG
Cuts at: 1243 1243
Size: 5144
EcoO109I rG'GnC_Cy
Cuts at: 622 678 679 915 926 1092 1236 2091 2283
Size: 56 1 236 11 166 144 855 192
Cuts at: 2283 622
Size: 3483
Fragments arranged by size:
3483 855 236 192 166 144 56 11
1
EcoRI G'AATT_C
Cuts at: 2148 2148
Size: 5144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 5144
FseI GG_CCGG'CC
Cuts at: 234 234
Size: 5144
FspI TGC'GCA
Cuts at: 2925 2925
Size: 5144
HaeII r_GCGC'y
Cuts at: 1056 1389 1558 2578 2826 3848 4827 1056
Size: 333 169 1020 248 1022 979 1373
Fragments arranged by size:
1373 1022 1020 979 333 248 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1024 1056 2986 3016 3018 3048 1024
Size: 32 1930 30 2 30 3120
Fragments arranged by size:
3120 1930 32 30 30 2
HincII GTy'rAC
Cuts at: 2183 2183
Size: 5144
KasI G'GCGC_C
Cuts at: 2574 2822 2574
Size: 248 4896
KpnI G_GTAC'C
Cuts at: 2164 2164
Size: 5144
MfeI C'AATT_G
Cuts at: 1333 1333
Size: 5144
MluI A'CGCG_T
Cuts at: 4577 4577
Size: 5144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 803 1694 3815 3999 803
Size: 891 2121 184 1948
Fragments arranged by size:
2121 1948 891 184
MscI TGG'CCA
Cuts at: 130 2905 130
Size: 2775 2369
MslI CAynn'nnrTG
Cuts at: 1993 3133 3260 1993
Size: 1140 127 3877
Fragments arranged by size:
3877 1140 127
MspA1I CmG'CkG
Cuts at: 342 352 465 488 669 718 1152 1212 1269
Size: 10 113 23 181 49 434 60 57
Cuts at: 1269 1278 1290 1299 1314 1356 1377 1413 1494
Size: 9 12 9 15 42 21 36 81
Cuts at: 1494 1692 1765 1809 1875 1902 1974 2255 2569
Size: 198 73 44 66 27 72 281 314
Cuts at: 2569 2929 3942 4187 342
Size: 360 1013 245 1299
Fragments arranged by size:
1299 1013 434 360 314 281 245 198
181 113 81 73 72 66 60 57
49 44 42 36 27 23 21 15
12 10 9 9
NaeI GCC'GGC
Cuts at: 232 3325 232
Size: 3093 2051
NarI GG'CG_CC
Cuts at: 2575 2823 2575
Size: 248 4896
NcoI C'CATG_G
Cuts at: 125 873 125
Size: 748 4396
NgoMIV G'CCGG_C
Cuts at: 230 3323 230
Size: 3093 2051
NspI r_CATG'y
Cuts at: 2197 2697 3604 2197
Size: 500 907 3737
Fragments arranged by size:
3737 907 500
PciI A'CATG_T
Cuts at: 3600 3600
Size: 5144
PfoI T'CCnGG_A
Cuts at: 1109 1736 1109
Size: 627 4517
PmeI GTTT'AAAC
Cuts at: 2143 2143
Size: 5144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 5112
PpuMI rG'GwC_Cy
Cuts at: 622 915 622
Size: 293 4851
PspOMI G'GGCC_C
Cuts at: 280 678 925 1280 1841 280
Size: 398 247 355 561 3583
Fragments arranged by size:
3583 561 398 355 247
PspXI vC'TCGA_Gb
Cuts at: 590 590
Size: 5144
PstI C_TGCA'G
Cuts at: 1298 1304 1319 1364 1667 1874 1979 2033 2191
Size: 6 15 45 303 207 105 54 158
Cuts at: 2191 2876 1298
Size: 685 3566
Fragments arranged by size:
3566 685 303 207 158 105 54 45
15 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5144
PvuII CAG'CTG
Cuts at: 352 669 718 1152 1290 1299 1314 1356 1377
Size: 317 49 434 138 9 15 42 21
Cuts at: 1377 1494 1692 1974 2569 2929 352
Size: 117 198 282 595 360 2567
Fragments arranged by size:
2567 595 434 360 317 282 198 138
117 49 42 21 15 9
RsrII CG'GwC_CG
Cuts at: 3339 3339
Size: 5144
SacI G_AGCT'C
Cuts at: 1421 1629 2066 2158 1421
Size: 208 437 92 4407
Fragments arranged by size:
4407 437 208 92
SacII CC_GC'GG
Cuts at: 489 489
Size: 5144
SalI G'TCGA_C
Cuts at: 2181 2181
Size: 5144
SanDI GG'GwC_CC
Cuts at: 915 915
Size: 5144
SapI GCTCTTCn'nnn_
Cuts at: 3167 3377 3167
Size: 210 4934
SbfI CC_TGCA'GG
Cuts at: 2191 2191
Size: 5144
ScaI AGT'ACT
Cuts at: 4661 4661
Size: 5144
SfcI C'TryA_G
Cuts at: 33 816 1294 1300 1315 1360 1609 1663 1870
Size: 783 478 6 15 45 249 54 207
Cuts at: 1870 1975 2029 2187 2872 3865 4056 33
Size: 105 54 158 685 993 191 1121
Fragments arranged by size:
1121 993 783 685 478 249 207 191
158 105 54 54 45 15 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2089 2089
Size: 5144
SfoI GGC'GCC
Cuts at: 2576 2824 2576
Size: 248 4896
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
SmaI CCC'GGG
Cuts at: 243 2059 2166 243
Size: 1816 107 3221
Fragments arranged by size:
3221 1816 107
SmlI C'TyrA_G
Cuts at: 590 1432 2299 3214 3706 3968 590
Size: 842 867 915 492 262 1766
Fragments arranged by size:
1766 915 867 842 492 262
SnaBI TAC'GTA
Cuts at: 2136 2136
Size: 5144
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5144
SphI G_CATG'C
Cuts at: 2197 2697 2197
Size: 500 4644
StyI C'CwwG_G
Cuts at: 125 361 873 978 1397 2278 2632 125
Size: 236 512 105 419 881 354 2637
Fragments arranged by size:
2637 881 512 419 354 236 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2727 2860 3319 3528 2727
Size: 133 459 209 4343
Fragments arranged by size:
4343 459 209 133
TatI w'GTAC_w
Cuts at: 4659 4659
Size: 5144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 328 360 853 885 1429 1461 4661 4693 328
Size: 32 493 32 544 32 3200 32 779
Fragments arranged by size:
3200 779 544 493 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 417 2941 417
Size: 2524 2620
XbaI T'CTAG_A
Cuts at: 2175 2175
Size: 5144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 274 940 2639 274
Size: 666 1699 2779
Fragments arranged by size:
2779 1699 666
XhoI C'TCGA_G
Cuts at: 590 590
Size: 5144
XmaI C'CCGG_G
Cuts at: 241 2057 2164 241
Size: 1816 107 3221
Fragments arranged by size:
3221 1816 107
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI BaeI BbsI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PsrI SexAI
SgrAI SrfI SspI StuI SwaI XmnI ZraI