Restriction map for
pF1KSDA1817
(Circular) (Six-base) MAPSORT of: pF1KSDA1817.seq Check: 4350 from: 1 to: 5735
pF1KSDA1817 5735 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 924 1980 2010 2610 924
Size: 1056 30 600 4049
Fragments arranged by size:
4049 1056 600 30
AccI GT'mk_AC
Cuts at: 1628 2773 1628
Size: 1145 4590
Acc65I G'GTAC_C
Cuts at: 2751 2751
Size: 5735
AclI AA'CG_TT
Cuts at: 5575 5575
Size: 5735
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 1016 3560 3992 4739 5007 433
Size: 583 2544 432 747 268 1161
Fragments arranged by size:
2544 1161 747 583 432 268
AflIII A'CryG_T
Cuts at: 1516 3087 4191 5168 1516
Size: 1571 1104 977 2083
Fragments arranged by size:
2083 1571 1104 977
AgeI A'CCGG_T
Cuts at: 4123 4123
Size: 5735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1261 2032 1261
Size: 771 4964
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1121 1153 1121
Size: 32 5703
AlwNI CAG_nnn'CTG
Cuts at: 1210 1402 2025 2388 2632 3040 4607 1210
Size: 192 623 363 244 408 1567 2338
Fragments arranged by size:
2338 1567 623 408 363 244 192
ApaI G_GGCC'C
Cuts at: 2139 2139
Size: 5735
ApaLI G'TGCA_C
Cuts at: 4505 4505
Size: 5735
ApoI r'AATT_y
Cuts at: 453 564 2739 5088 5344 453
Size: 111 2175 2349 256 844
Fragments arranged by size:
2349 2175 844 256 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 5735
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5735
AvaI C'yCGr_G
Cuts at: 767 866 893 1022 1469 1524 1807 2227 2755
Size: 99 27 129 447 55 283 420 528
Cuts at: 2755 767
Size: 3747
Fragments arranged by size:
3747 528 447 420 283 129 99 55
27
BamHI G'GATC_C
Cuts at: 2760 2760
Size: 5735
BanI G'GyrC_C
Cuts at: 685 1183 1995 2751 3165 3413 3448 685
Size: 498 812 756 414 248 35 2972
Fragments arranged by size:
2972 812 756 498 414 248 35
BanII G_rGCy'C
Cuts at: 214 799 880 907 984 1548 1940 2139 2306
Size: 585 81 27 77 564 392 199 167
Cuts at: 2306 2347 2749 3779 214
Size: 41 402 1030 2170
Fragments arranged by size:
2170 1030 585 564 402 392 199 167
81 77 41 27
BbeI G_GCGC'C
Cuts at: 3169 3417 3169
Size: 248 5487
BbvCI CC'TCA_GC
Cuts at: 178 1420 2513 178
Size: 1242 1093 3400
Fragments arranged by size:
3400 1242 1093
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2218 2252 2218
Size: 34 5701
BciVI GTATCCnnnnn_n'
Cuts at: 3633 4394 5735 3633
Size: 761 1341 3633
Fragments arranged by size:
3633 1341 761
BclI T'GATC_A
Cuts at: 3255 3255
Size: 5735
BglI GCCn_nnn'nGGC
Cuts at: 1313 2134 1313
Size: 821 4914
BglII A'GATC_T
Cuts at: 2261 3250 5287 2261
Size: 989 2037 2709
Fragments arranged by size:
2709 2037 989
BlpI GC'TnA_GC
Cuts at: 2389 2847 2389
Size: 458 5277
Bme1580I G_kGCm'C
Cuts at: 688 1346 2139 2241 3360 3453 4509 688
Size: 658 793 102 1119 93 1056 1914
Fragments arranged by size:
1914 1119 1056 793 658 102 93
BmrI ACTGGGnnnn_n'
Cuts at: 425 2947 3028 3363 5507 425
Size: 2522 81 335 2144 653
Fragments arranged by size:
2522 2144 653 335 81
BmtI G_CTAG'C
Cuts at: 2287 2287
Size: 5735
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 186 2366 4102 186
Size: 2180 1736 1819
Fragments arranged by size:
2180 1819 1736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 422 678 739 1153 1187 1483 1741 373
Size: 49 256 61 414 34 296 258 4367
Fragments arranged by size:
4367 414 296 258 256 61 49 34
Bpu10I CC'TnA_GC
Cuts at: 178 1420 2513 178
Size: 1242 1093 3400
Fragments arranged by size:
3400 1242 1093
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 2275 2299 2911 3790 4282 4580 1162
Size: 1113 24 612 879 492 298 2317
Fragments arranged by size:
2317 1113 879 612 492 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 174 436 486 745 920 1721 1733 1762 2398
Size: 262 50 259 175 801 12 29 636
Cuts at: 2398 5439 174
Size: 3041 470
Fragments arranged by size:
3041 801 636 470 262 259 175 50
29 12
BsaAI yAC'GTr
Cuts at: 1517 2727 3088 1517
Size: 1210 361 4164
Fragments arranged by size:
4164 1210 361
BsaBI GATnn'nnATC
Cuts at: 6 3249 5286 6
Size: 3243 2037 455
Fragments arranged by size:
3243 2037 455
BsaHI Gr'CG_yC
Cuts at: 3166 3414 4116 5612 3166
Size: 248 702 1496 3289
Fragments arranged by size:
3289 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1820 2924 3445 4123 4397 4544 4955 1820
Size: 1104 521 678 274 147 411 2600
Fragments arranged by size:
2600 1104 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 190 220 190
Size: 30 5705
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1825 2530 1825
Size: 705 5030
BseYI C'CCAG_C
Cuts at: 331 468 799 826 853 880 1345 1734 2444
Size: 137 331 27 27 27 465 389 710
Cuts at: 2444 3160 3918 4495 4991 331
Size: 716 758 577 496 1075
Fragments arranged by size:
1075 758 716 710 577 496 465 389
331 137 27 27 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 511 1267 2528 494
Size: 17 756 1261 3701
Fragments arranged by size:
3701 1261 756 17
BsiEI CG_ry'CG
Cuts at: 104 266 1483 3277 3323 4531 104
Size: 162 1217 1794 46 1208 1308
Fragments arranged by size:
1794 1308 1217 1208 162 46
BsiHKAI G_wGCw'C
Cuts at: 438 501 727 943 1548 1940 2306 2692 2749
Size: 63 226 216 605 392 366 386 57
Cuts at: 2749 3527 3717 4509 438
Size: 778 190 792 1664
Fragments arranged by size:
1664 792 778 605 392 386 366 226
216 190 63 57
BsmI GAATG_Cn'
Cuts at: 1445 1445
Size: 5735
Bsp1286I G_dGCh'C
Cuts at: 214 438 501 688 727 799 880 907 943
Size: 224 63 187 39 72 81 27 36
Cuts at: 943 984 1346 1548 1940 2139 2241 2306 2347
Size: 41 362 202 392 199 102 65 41
Cuts at: 2347 2692 2749 3360 3453 3527 3717 3779 4509
Size: 345 57 611 93 74 190 62 730
Cuts at: 4509 214
Size: 1440
Fragments arranged by size:
1440 730 611 392 362 345 224 202
199 190 187 102 93 81 74 72
65 63 62 57 41 41 39 36
27
BspEI T'CCGG_A
Cuts at: 4955 4955
Size: 5735
BspHI T'CATG_A
Cuts at: 4911 4911
Size: 5735
BspMI ACCTGCnnnn'nnnn_
Cuts at: 156 197 924 1202 1703 1980 2010 2047 2610
Size: 41 727 278 501 277 30 37 563
Cuts at: 2610 2785 2999 3301 3682 156
Size: 175 214 302 381 2209
Fragments arranged by size:
2209 727 563 501 381 302 278 277
214 175 41 37 30
BsrBI CCG'CTC
Cuts at: 50 1095 4027 4081 5565 5730 50
Size: 1045 2932 54 1484 165 55
Fragments arranged by size:
2932 1484 1045 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3647 3647
Size: 5735
BsrFI r'CCGG_y
Cuts at: 929 1316 3733 3914 4123 929
Size: 387 2417 181 209 2541
Fragments arranged by size:
2541 2417 387 209 181
BssSI C'ACGA_G
Cuts at: 4006 4364 4006
Size: 358 5377
BstAPI GCAn_nnn'nTGC
Cuts at: 1862 1865 1915 1995 2632 3112 1862
Size: 3 50 80 637 480 4485
Fragments arranged by size:
4485 637 480 80 50 3
BstBI TT'CG_AA
Cuts at: 110 4096 110
Size: 3986 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 841 1698 1788 2146 841
Size: 857 90 358 4430
Fragments arranged by size:
4430 857 358 90
BstYI r'GATC_y
Cuts at: 1 1961 2261 2760 3227 3250 3585 3831 4843
Size: 1960 300 499 467 23 335 246 1012
Cuts at: 4843 4929 4941 5287 1
Size: 86 12 346 449
Fragments arranged by size:
1960 1012 499 467 449 346 335 300
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2057 2057
Size: 5735
BtgI C'CryG_G
Cuts at: 782 782
Size: 5735
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3685 3866 4029 3685
Size: 181 163 5391
Fragments arranged by size:
5391 181 163
BtsI GCAGTG_nn'
Cuts at: 246 302 402 1801 2218 2978 246
Size: 56 100 1399 417 760 3003
Fragments arranged by size:
3003 1399 760 417 100 56
DraI TTT'AAA
Cuts at: 2734 2734
Size: 5735
DrdI GACnn_nn'nnGTC
Cuts at: 3441 4299 3441
Size: 858 4877
EaeI y'GGCC_r
Cuts at: 263 843 1212 1318 1790 2114 2219 2327 2462
Size: 580 369 106 472 324 105 108 135
Cuts at: 2462 3320 3494 3885 3912 5257 263
Size: 858 174 391 27 1345 741
Fragments arranged by size:
1345 858 741 580 472 391 369 324
174 135 108 106 105 27
EagI C'GGCC_G
Cuts at: 263 3320 263
Size: 3057 2678
EarI CTCTTCn'nnn_
Cuts at: 155 489 743 2468 3758 3968 155
Size: 334 254 1725 1290 210 1922
Fragments arranged by size:
1922 1725 1290 334 254 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3943 4253 4399 5545 3943
Size: 310 146 1146 4133
Fragments arranged by size:
4133 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1546 1938 2304 2747 1546
Size: 392 366 443 4534
Fragments arranged by size:
4534 443 392 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 422 433 678 739 1016 1153 1187 1483
Size: 49 11 245 61 277 137 34 296
Cuts at: 1483 1741 3560 3992 4739 5007 373
Size: 258 1819 432 747 268 1101
Fragments arranged by size:
1819 1101 747 432 296 277 268 258
245 137 61 49 34 11
EcoNI CCTnn'n_nnAGG
Cuts at: 193 193
Size: 5735
EcoO109I rG'GnC_Cy
Cuts at: 1000 1027 1255 1607 1702 1777 2012 2874 1000
Size: 27 228 352 95 75 235 862 3861
Fragments arranged by size:
3861 862 352 235 228 95 75 27
EcoRI G'AATT_C
Cuts at: 453 2739 453
Size: 2286 3449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3754 3754
Size: 5735
FseI GG_CCGG'CC
Cuts at: 1320 1320
Size: 5735
FspI TGC'GCA
Cuts at: 3516 3516
Size: 5735
HaeII r_GCGC'y
Cuts at: 2451 3169 3417 4439 5418 2451
Size: 718 248 1022 979 2768
Fragments arranged by size:
2768 1022 979 718 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1465 1497 1594 1626 1924 1956 3577 3607 3609
Size: 32 97 32 298 32 1621 30 2
Cuts at: 3609 3639 1465
Size: 30 3561
Fragments arranged by size:
3561 1621 298 97 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2774 2774
Size: 5735
HindIII A'AGCT_T
Cuts at: 217 958 2275 217
Size: 741 1317 3677
Fragments arranged by size:
3677 1317 741
KasI G'GCGC_C
Cuts at: 3165 3413 3165
Size: 248 5487
KpnI G_GTAC'C
Cuts at: 2755 2755
Size: 5735
MluI A'CGCG_T
Cuts at: 5168 5168
Size: 5735
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4406 4590 4406
Size: 184 5551
MscI TGG'CCA
Cuts at: 845 2116 2221 2329 2464 3496 845
Size: 1271 105 108 135 1032 3084
Fragments arranged by size:
3084 1271 1032 135 108 105
MslI CAynn'nnrTG
Cuts at: 2144 3724 3851 2144
Size: 1580 127 4028
Fragments arranged by size:
4028 1580 127
MspA1I CmG'CkG
Cuts at: 468 717 803 830 884 1217 1223 1286 2512
Size: 249 86 27 54 333 6 63 1226
Cuts at: 2512 2632 2846 3160 3520 4533 4778 468
Size: 120 214 314 360 1013 245 1425
Fragments arranged by size:
1425 1226 1013 360 333 314 249 245
214 120 86 63 54 27 6
NaeI GCC'GGC
Cuts at: 931 1318 3916 931
Size: 387 2598 2750
Fragments arranged by size:
2750 2598 387
NarI GG'CG_CC
Cuts at: 3166 3414 3166
Size: 248 5487
NcoI C'CATG_G
Cuts at: 782 782
Size: 5735
NgoMIV G'CCGG_C
Cuts at: 929 1316 3914 929
Size: 387 2598 2750
Fragments arranged by size:
2750 2598 387
NheI G'CTAG_C
Cuts at: 2283 2283
Size: 5735
NspI r_CATG'y
Cuts at: 1266 1653 2358 2788 3288 4195 1266
Size: 387 705 430 500 907 2806
Fragments arranged by size:
2806 907 705 500 430 387
PasI CC'CwG_GG
Cuts at: 1644 1658 1644
Size: 14 5721
PciI A'CATG_T
Cuts at: 4191 4191
Size: 5735
PflMI CCAn_nnn'nTGG
Cuts at: 658 1210 1402 658
Size: 552 192 4991
Fragments arranged by size:
4991 552 192
PmeI GTTT'AAAC
Cuts at: 2734 2734
Size: 5735
PmlI CAC'GTG
Cuts at: 1517 1517
Size: 5735
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4874 4906 4874
Size: 32 5703
PpuMI rG'GwC_Cy
Cuts at: 1027 1702 2012 1027
Size: 675 310 4750
Fragments arranged by size:
4750 675 310
PspOMI G'GGCC_C
Cuts at: 2135 2135
Size: 5735
PspXI vC'TCGA_Gb
Cuts at: 893 893
Size: 5735
PstI C_TGCA'G
Cuts at: 677 794 1112 1222 2021 2044 2637 2782 3467
Size: 117 318 110 799 23 593 145 685
Cuts at: 3467 677
Size: 2945
Fragments arranged by size:
2945 799 685 593 318 145 117 110
23
PvuI CG_AT'CG
Cuts at: 104 1483 104
Size: 1379 4356
PvuII CAG'CTG
Cuts at: 468 717 803 830 884 1223 1286 2512 2632
Size: 249 86 27 54 339 63 1226 120
Cuts at: 2632 3160 3520 468
Size: 528 360 2683
Fragments arranged by size:
2683 1226 528 360 339 249 120 86
63 54 27
RsrII CG'GwC_CG
Cuts at: 3930 3930
Size: 5735
SacI G_AGCT'C
Cuts at: 1548 1940 2306 2749 1548
Size: 392 366 443 4534
Fragments arranged by size:
4534 443 392 366
SalI G'TCGA_C
Cuts at: 2772 2772
Size: 5735
SanDI GG'GwC_CC
Cuts at: 1702 1702
Size: 5735
SapI GCTCTTCn'nnn_
Cuts at: 155 3758 3968 155
Size: 3603 210 1922
Fragments arranged by size:
3603 1922 210
SbfI CC_TGCA'GG
Cuts at: 2021 2782 2021
Size: 761 4974
ScaI AGT'ACT
Cuts at: 5252 5252
Size: 5735
SfcI C'TryA_G
Cuts at: 33 271 623 673 790 1108 1218 1575 2017
Size: 238 352 50 117 318 110 357 442
Cuts at: 2017 2040 2633 2778 3463 4456 4647 33
Size: 23 593 145 685 993 191 1121
Fragments arranged by size:
1121 993 685 593 442 357 352 318
238 191 145 117 110 50 23
SfoI GGC'GCC
Cuts at: 3167 3415 3167
Size: 248 5487
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5735
SmaI CCC'GGG
Cuts at: 2757 2757
Size: 5735
SmlI C'TyrA_G
Cuts at: 866 893 1141 2278 2290 2890 3805 4297 4559
Size: 27 248 1137 12 600 915 492 262
Cuts at: 4559 866
Size: 2042
Fragments arranged by size:
2042 1137 915 600 492 262 248 27
12
SnaBI TAC'GTA
Cuts at: 2727 2727
Size: 5735
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5735
SphI G_CATG'C
Cuts at: 1653 2788 3288 1653
Size: 1135 500 4100
Fragments arranged by size:
4100 1135 500
StuI AGG'CCT
Cuts at: 2062 2062
Size: 5735
StyI C'CwwG_G
Cuts at: 194 699 782 1667 1697 2465 2869 3223 194
Size: 505 83 885 30 768 404 354 2706
Fragments arranged by size:
2706 885 768 505 404 354 83 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 460 516 646 744 1345 2713 3318 3451 3910
Size: 56 130 98 601 1368 605 133 459
Cuts at: 3910 4119 460
Size: 209 2076
Fragments arranged by size:
2076 1368 605 601 459 209 133 130
98 56
TatI w'GTAC_w
Cuts at: 5250 5250
Size: 5735
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2637 2669 5252 5284 2637
Size: 32 2583 32 3088
Fragments arranged by size:
3088 2583 32 32
Tth111I GACn'n_nGTC
Cuts at: 1372 3532 1372
Size: 2160 3575
XbaI T'CTAG_A
Cuts at: 2766 2766
Size: 5735
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 446 3230 446
Size: 2784 2951
XhoI C'TCGA_G
Cuts at: 866 893 866
Size: 27 5708
XmaI C'CCGG_G
Cuts at: 2755 2755
Size: 5735
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BbsI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PfoI PshAI PsiI
PsrI SacII SexAI SfiI SgrAI SrfI SspI SwaI
XmnI ZraI