Restriction map for
pF1KSDA1819
(Circular) (Six-base) MAPSORT of: pF1KSDA1819.seq Check: 3639 from: 1 to: 6596
pF1KSDA1819 6596 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 545 3096 545
Size: 2551 4045
AatII G_ACGT'C
Cuts at: 3058 3058
Size: 6596
AccI GT'mk_AC
Cuts at: 2665 3634 2665
Size: 969 5627
Acc65I G'GTAC_C
Cuts at: 3612 3612
Size: 6596
AclI AA'CG_TT
Cuts at: 6436 6436
Size: 6596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 2158 2176 2753 4421 4853 5600 5868 1325
Size: 833 18 577 1668 432 747 268 2053
Fragments arranged by size:
2053 1668 833 747 577 432 268 18
AflIII A'CryG_T
Cuts at: 1664 3948 5052 6029 1664
Size: 2284 1104 977 2231
Fragments arranged by size:
2284 2231 1104 977
AgeI A'CCGG_T
Cuts at: 4984 4984
Size: 6596
AleI CACnn'nnGTG
Cuts at: 3425 3425
Size: 6596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2573 2573
Size: 6596
AlwNI CAG_nnn'CTG
Cuts at: 354 537 1050 2448 3114 3526 3901 5468 354
Size: 183 513 1398 666 412 375 1567 1482
Fragments arranged by size:
1567 1482 1398 666 513 412 375 183
ApaI G_GGCC'C
Cuts at: 1252 3475 1252
Size: 2223 4373
ApaLI G'TGCA_C
Cuts at: 220 5366 220
Size: 5146 1450
ApoI r'AATT_y
Cuts at: 740 2931 3600 5949 6205 740
Size: 2191 669 2349 256 1131
Fragments arranged by size:
2349 2191 1131 669 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6596
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6596
AvaI C'yCGr_G
Cuts at: 213 3616 213
Size: 3403 3193
AvrII C'CTAG_G
Cuts at: 1183 1183
Size: 6596
BamHI G'GATC_C
Cuts at: 1032 3621 1032
Size: 2589 4007
BanI G'GyrC_C
Cuts at: 1697 3612 4026 4274 4309 1697
Size: 1915 414 248 35 3984
Fragments arranged by size:
3984 1915 414 248 35
BanII G_rGCy'C
Cuts at: 191 205 252 322 1252 1609 1778 2020 3475
Size: 14 47 70 930 357 169 242 1455
Cuts at: 3475 3610 4640 191
Size: 135 1030 2147
Fragments arranged by size:
2147 1455 1030 930 357 242 169 135
70 47 14
BbeI G_GCGC'C
Cuts at: 4030 4278 4030
Size: 248 6348
BbsI GAAGACnn'nnnn_
Cuts at: 3523 3523
Size: 6596
BbvCI CC'TCA_GC
Cuts at: 1265 1270 1683 3448 1265
Size: 5 413 1765 4413
Fragments arranged by size:
4413 1765 413 5
BciVI GTATCCnnnnn_n'
Cuts at: 1425 2221 4494 5255 6596 1425
Size: 796 2273 761 1341 1425
Fragments arranged by size:
2273 1425 1341 796 761
BclI T'GATC_A
Cuts at: 1112 1214 2984 4116 1112
Size: 102 1770 1132 3592
Fragments arranged by size:
3592 1770 1132 102
BglI GCCn_nnn'nGGC
Cuts at: 181 1256 1404 181
Size: 1075 148 5373
Fragments arranged by size:
5373 1075 148
BglII A'GATC_T
Cuts at: 4111 6148 4111
Size: 2037 4559
BlpI GC'TnA_GC
Cuts at: 1361 3277 3708 1361
Size: 1916 431 4249
Fragments arranged by size:
4249 1916 431
Bme1580I G_kGCm'C
Cuts at: 224 1252 1700 3475 4221 4314 5370 224
Size: 1028 448 1775 746 93 1056 1450
Fragments arranged by size:
1775 1450 1056 1028 746 448 93
BmrI ACTGGGnnnn_n'
Cuts at: 2285 2614 3021 3808 3889 4224 6368 2285
Size: 329 407 787 81 335 2144 2513
Fragments arranged by size:
2513 2144 787 407 335 329 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 777 1376 4963 777
Size: 599 3587 2410
Fragments arranged by size:
3587 2410 599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1037 1123 2419 2495 625
Size: 412 86 1296 76 4726
Fragments arranged by size:
4726 1296 412 86 76
Bpu10I CC'TnA_GC
Cuts at: 280 355 1037 1265 1270 1683 2155 3448 280
Size: 75 682 228 5 413 472 1293 3428
Fragments arranged by size:
3428 1293 682 472 413 228 75 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3005 3772 4651 5143 5441 3005
Size: 767 879 492 298 4160
Fragments arranged by size:
4160 879 767 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2919 3189 6300 2919
Size: 270 3111 3215
Fragments arranged by size:
3215 3111 270
BsaAI yAC'GTr
Cuts at: 3588 3949 3588
Size: 361 6235
BsaBI GATnn'nnATC
Cuts at: 6 4110 6147 6
Size: 4104 2037 455
Fragments arranged by size:
4104 2037 455
BsaHI Gr'CG_yC
Cuts at: 3055 3364 4027 4275 4977 6473 3055
Size: 309 663 248 702 1496 3178
Fragments arranged by size:
3178 1496 702 663 309 248
BsaWI w'CCGG_w
Cuts at: 1378 3785 4306 4984 5258 5405 5816 1378
Size: 2407 521 678 274 147 411 2158
Fragments arranged by size:
2407 2158 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 423 453 864 894 1314 1344 2221 2251 2329
Size: 30 411 30 420 30 877 30 78
Cuts at: 2329 2359 2417 2447 423
Size: 30 58 30 4572
Fragments arranged by size:
4572 877 420 411 78 58 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1188 1253 1512 3152 1188
Size: 65 259 1640 4632
Fragments arranged by size:
4632 1640 259 65
BseYI C'CCAG_C
Cuts at: 1424 1567 1764 2348 3367 4021 4779 5356 5852
Size: 143 197 584 1019 654 758 577 496
Cuts at: 5852 1424
Size: 2168
Fragments arranged by size:
2168 1019 758 654 584 577 496 197
143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2987 2987
Size: 6596
BsiEI CG_ry'CG
Cuts at: 104 4138 4184 5392 104
Size: 4034 46 1208 1308
Fragments arranged by size:
4034 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 224 322 1180 1609 2020 3610 4388 4578 5370
Size: 98 858 429 411 1590 778 190 792
Cuts at: 5370 224
Size: 1450
Fragments arranged by size:
1590 1450 858 792 778 429 411 190
98
BsmI GAATG_Cn'
Cuts at: 1294 2690 1294
Size: 1396 5200
BsmBI CGTCTCn'nnnn_
Cuts at: 1001 1334 1001
Size: 333 6263
Bsp1286I G_dGCh'C
Cuts at: 191 205 224 252 322 1180 1252 1609 1700
Size: 14 19 28 70 858 72 357 91
Cuts at: 1700 1778 2020 3475 3610 4221 4314 4388 4578
Size: 78 242 1455 135 611 93 74 190
Cuts at: 4578 4640 5370 191
Size: 62 730 1417
Fragments arranged by size:
1455 1417 858 730 611 357 242 190
135 93 91 78 74 72 70 62
28 19 14
BspEI T'CCGG_A
Cuts at: 1378 5816 1378
Size: 4438 2158
BspHI T'CATG_A
Cuts at: 5772 5772
Size: 6596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 545 3096 3646 3860 4162 4543 545
Size: 2551 550 214 302 381 2598
Fragments arranged by size:
2598 2551 550 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4888 4942 6426 6591 50
Size: 4838 54 1484 165 55
Fragments arranged by size:
4838 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 894 2213 4508 894
Size: 1319 2295 2982
Fragments arranged by size:
2982 2295 1319
BsrFI r'CCGG_y
Cuts at: 1675 4594 4775 4984 1675
Size: 2919 181 209 3287
Fragments arranged by size:
3287 2919 209 181
BsrGI T'GTAC_A
Cuts at: 363 1667 363
Size: 1304 5292
BssSI C'ACGA_G
Cuts at: 4867 5225 4867
Size: 358 6238
BstAPI GCAn_nnn'nTGC
Cuts at: 537 2445 3973 537
Size: 1908 1528 3160
Fragments arranged by size:
3160 1908 1528
BstBI TT'CG_AA
Cuts at: 110 4957 110
Size: 4847 1749
BstEII G'GTnAC_C
Cuts at: 2159 2159
Size: 6596
BstYI r'GATC_y
Cuts at: 1 1032 1731 2899 3621 4088 4111 4446 4692
Size: 1031 699 1168 722 467 23 335 246
Cuts at: 4692 5704 5790 5802 6148 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1168 1031 1012 722 699 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1254 1727 2856 3247 1254
Size: 473 1129 391 4603
Fragments arranged by size:
4603 1129 473 391
BtgI C'CryG_G
Cuts at: 125 1199 1301 1769 2842 3236 125
Size: 1074 102 468 1073 394 3485
Fragments arranged by size:
3485 1074 1073 468 394 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4546 4727 4890 4546
Size: 181 163 6252
Fragments arranged by size:
6252 181 163
BtsI GCAGTG_nn'
Cuts at: 416 570 3112 3161 3191 3432 3839 416
Size: 154 2542 49 30 241 407 3173
Fragments arranged by size:
3173 2542 407 241 154 49 30
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 540 575 540
Size: 35 6561
DraI TTT'AAA
Cuts at: 3595 3595
Size: 6596
DraIII CAC_nnn'GTG
Cuts at: 2268 2268
Size: 6596
DrdI GACnn_nn'nnGTC
Cuts at: 4302 5160 4302
Size: 858 5738
EaeI y'GGCC_r
Cuts at: 372 966 1304 1638 2845 4181 4355 4746 4773
Size: 594 338 334 1207 1336 174 391 27
Cuts at: 4773 6118 372
Size: 1345 850
Fragments arranged by size:
1345 1336 1207 850 594 391 338 334
174 27
EagI C'GGCC_G
Cuts at: 4181 4181
Size: 6596
EarI CTCTTCn'nnn_
Cuts at: 4619 4829 4619
Size: 210 6386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4804 5114 5260 6406 4804
Size: 310 146 1146 4994
Fragments arranged by size:
4994 1146 310 146
EcoICRI GAG'CTC
Cuts at: 320 1607 2018 3608 320
Size: 1287 411 1590 3308
Fragments arranged by size:
3308 1590 1287 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1037 1123 1325 2158 2176 2419 2495 2753
Size: 412 86 202 833 18 243 76 258
Cuts at: 2753 4421 4853 5600 5868 625
Size: 1668 432 747 268 1353
Fragments arranged by size:
1668 1353 833 747 432 412 268 258
243 202 86 76 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1370 2855 1370
Size: 1485 5111
EcoO109I rG'GnC_Cy
Cuts at: 150 173 818 1187 1248 1249 1274 2287 3241
Size: 23 645 369 61 1 25 1013 954
Cuts at: 3241 3472 3735 150
Size: 231 263 3011
Fragments arranged by size:
3011 1013 954 645 369 263 231 61
25 23 1
EcoRI G'AATT_C
Cuts at: 740 3600 740
Size: 2860 3736
EcoRV GAT'ATC
Cuts at: 732 732
Size: 6596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2165 2180 4615 2165
Size: 15 2435 4146
Fragments arranged by size:
4146 2435 15
FseI GG_CCGG'CC
Cuts at: 1679 1679
Size: 6596
FspI TGC'GCA
Cuts at: 4377 4377
Size: 6596
HaeII r_GCGC'y
Cuts at: 142 243 4030 4278 5300 6279 142
Size: 101 3787 248 1022 979 459
Fragments arranged by size:
3787 1022 979 459 248 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 843 875 928 960 2219 2241 2248 2251 2273
Size: 32 53 32 1259 22 7 3 22
Cuts at: 2273 2280 4438 4468 4470 4500 843
Size: 7 2158 30 2 30 2939
Fragments arranged by size:
2939 2158 1259 53 32 32 30 30
22 22 7 7 3 2
HincII GTy'rAC
Cuts at: 672 3176 3635 672
Size: 2504 459 3633
Fragments arranged by size:
3633 2504 459
HindIII A'AGCT_T
Cuts at: 2589 2589
Size: 6596
HpaI GTT'AAC
Cuts at: 672 3176 672
Size: 2504 4092
KasI G'GCGC_C
Cuts at: 4026 4274 4026
Size: 248 6348
KpnI G_GTAC'C
Cuts at: 3616 3616
Size: 6596
MfeI C'AATT_G
Cuts at: 2371 2782 2809 2920 2371
Size: 411 27 111 6047
Fragments arranged by size:
6047 411 111 27
MluI A'CGCG_T
Cuts at: 6029 6029
Size: 6596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3017 5267 5451 3017
Size: 2250 184 4162
Fragments arranged by size:
4162 2250 184
MscI TGG'CCA
Cuts at: 374 968 1306 4357 374
Size: 594 338 3051 2613
Fragments arranged by size:
3051 2613 594 338
MslI CAynn'nnrTG
Cuts at: 962 1075 1703 3425 4585 4712 962
Size: 113 628 1722 1160 127 2846
Fragments arranged by size:
2846 1722 1160 628 127 113
MspA1I CmG'CkG
Cuts at: 1269 1285 1637 3707 4021 4381 5394 5639 1269
Size: 16 352 2070 314 360 1013 245 2226
Fragments arranged by size:
2226 2070 1013 360 352 314 245 16
NaeI GCC'GGC
Cuts at: 1677 4777 1677
Size: 3100 3496
NarI GG'CG_CC
Cuts at: 4027 4275 4027
Size: 248 6348
NcoI C'CATG_G
Cuts at: 125 1199 1301 1769 3236 125
Size: 1074 102 468 1467 3485
Fragments arranged by size:
3485 1467 1074 468 102
NgoMIV G'CCGG_C
Cuts at: 1675 4775 1675
Size: 3100 3496
NspI r_CATG'y
Cuts at: 1465 1668 2958 3649 4149 5056 1465
Size: 203 1290 691 500 907 3005
Fragments arranged by size:
3005 1290 907 691 500 203
PasI CC'CwG_GG
Cuts at: 607 1689 607
Size: 1082 5514
PciI A'CATG_T
Cuts at: 1664 5052 1664
Size: 3388 3208
PflMI CCAn_nnn'nTGG
Cuts at: 1285 2326 2610 2946 1285
Size: 1041 284 336 4935
Fragments arranged by size:
4935 1041 336 284
PmeI GTTT'AAAC
Cuts at: 3595 3595
Size: 6596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5735 5767 5735
Size: 32 6564
PpuMI rG'GwC_Cy
Cuts at: 818 3241 818
Size: 2423 4173
PshAI GACnn'nnGTC
Cuts at: 1157 2496 1157
Size: 1339 5257
PsiI TTA'TAA
Cuts at: 2519 2519
Size: 6596
PspOMI G'GGCC_C
Cuts at: 1248 3471 1248
Size: 2223 4373
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3568 3600 3568
Size: 32 6564
PstI C_TGCA'G
Cuts at: 768 1046 3063 3093 3643 4328 768
Size: 278 2017 30 550 685 3036
Fragments arranged by size:
3036 2017 685 550 278 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6596
PvuII CAG'CTG
Cuts at: 1269 1285 4021 4381 1269
Size: 16 2736 360 3484
Fragments arranged by size:
3484 2736 360 16
RsrII CG'GwC_CG
Cuts at: 4791 4791
Size: 6596
SacI G_AGCT'C
Cuts at: 322 1609 2020 3610 322
Size: 1287 411 1590 3308
Fragments arranged by size:
3308 1590 1287 411
SalI G'TCGA_C
Cuts at: 3633 3633
Size: 6596
SanDI GG'GwC_CC
Cuts at: 3241 3241
Size: 6596
SapI GCTCTTCn'nnn_
Cuts at: 4619 4829 4619
Size: 210 6386
SbfI CC_TGCA'GG
Cuts at: 3643 3643
Size: 6596
ScaI AGT'ACT
Cuts at: 6113 6113
Size: 6596
SexAI A'CCwGG_T
Cuts at: 1539 1539
Size: 6596
SfcI C'TryA_G
Cuts at: 33 431 764 1042 2395 2699 3059 3089 3639
Size: 398 333 278 1353 304 360 30 550
Cuts at: 3639 4324 5317 5508 33
Size: 685 993 191 1121
Fragments arranged by size:
1353 1121 993 685 550 398 360 333
304 278 191 30
SfoI GGC'GCC
Cuts at: 4028 4276 4028
Size: 248 6348
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6596
SmaI CCC'GGG
Cuts at: 3618 3618
Size: 6596
SmlI C'TyrA_G
Cuts at: 3020 3751 4666 5158 5420 3020
Size: 731 915 492 262 4196
Fragments arranged by size:
4196 915 731 492 262
SnaBI TAC'GTA
Cuts at: 3588 3588
Size: 6596
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6596
SphI G_CATG'C
Cuts at: 1465 2958 3649 4149 1465
Size: 1493 691 500 3912
Fragments arranged by size:
3912 1493 691 500
SspI AAT'ATT
Cuts at: 987 987
Size: 6596
StyI C'CwwG_G
Cuts at: 125 192 404 1183 1199 1301 1769 3236 3730
Size: 67 212 779 16 102 468 1467 494
Cuts at: 3730 4084 125
Size: 354 2637
Fragments arranged by size:
2637 1467 779 494 468 354 212 102
67 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1778 1900 3399 4179 4312 4771 4980 1778
Size: 122 1499 780 133 459 209 3394
Fragments arranged by size:
3394 1499 780 459 209 133 122
TatI w'GTAC_w
Cuts at: 363 1667 6111 363
Size: 1304 4444 848
Fragments arranged by size:
4444 1304 848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1314 1346 6113 6145 1314
Size: 32 4767 32 1765
Fragments arranged by size:
4767 1765 32 32
Tth111I GACn'n_nGTC
Cuts at: 800 942 4393 800
Size: 142 3451 3003
Fragments arranged by size:
3451 3003 142
XbaI T'CTAG_A
Cuts at: 3627 3627
Size: 6596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 790 2220 3223 4091 708
Size: 82 1430 1003 868 3213
Fragments arranged by size:
3213 1430 1003 868 82
XmaI C'CCGG_G
Cuts at: 3616 3616
Size: 6596
ZraI GAC'GTC
Cuts at: 3056 3056
Size: 6596
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI BaeI BcgI BfrBI
BmgBI BmtI BsiWI BssHII BstXI BstZ17I ClaI FspAI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PspXI SacII SfiI SgrAI SrfI StuI SwaI XhoI
XmnI