Restriction map for
pF1KSDA1830
(Circular) (Six-base) MAPSORT of: pF1KSDA1830.seq Check: 8131 from: 1 to: 4499
pF1KSDA1830 4499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AccI GT'mk_AC
Cuts at: 1537 1537
Size: 4499
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 623 2324 2756 3503 3771 383
Size: 240 1701 432 747 268 1111
Fragments arranged by size:
1701 1111 747 432 268 240
AflIII A'CryG_T
Cuts at: 1851 2955 3932 1851
Size: 1104 977 2418
Fragments arranged by size:
2418 1104 977
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 1804 3371 1804
Size: 1567 2932
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 1503 3852 4108 1503
Size: 2349 256 1894
Fragments arranged by size:
2349 1894 256
AseI AT'TA_AT
Cuts at: 20 1133 20
Size: 1113 3386
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 1519 1519
Size: 4499
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 1515 1929 2177 2212 1515
Size: 414 248 35 3802
Fragments arranged by size:
3802 414 248 35
BanII G_rGCy'C
Cuts at: 522 1246 1421 1460 1513 2543 522
Size: 724 175 39 53 1030 2478
Fragments arranged by size:
2478 1030 724 175 53 39
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BbsI GAAGACnn'nnnn_
Cuts at: 1121 1121
Size: 4499
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglII A'GATC_T
Cuts at: 2014 4051 2014
Size: 2037 2462
BlpI GC'TnA_GC
Cuts at: 1611 1611
Size: 4499
Bme1580I G_kGCm'C
Cuts at: 2124 2217 3273 2124
Size: 93 1056 3350
Fragments arranged by size:
3350 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1291 1711 1792 2127 4271 1291
Size: 420 81 335 2144 1519
Fragments arranged by size:
2144 1519 420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 335
Size: 4499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1675 2554 3046 3344 349
Size: 1326 879 492 298 1504
Fragments arranged by size:
1504 1326 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 953 4203 953
Size: 3250 1249
BsaAI yAC'GTr
Cuts at: 1491 1852 1491
Size: 361 4138
BsaBI GATnn'nnATC
Cuts at: 6 776 2013 4050 6
Size: 770 1237 2037 455
Fragments arranged by size:
2037 1237 770 455
BsaHI Gr'CG_yC
Cuts at: 1930 2178 2880 4376 1930
Size: 248 702 1496 2053
Fragments arranged by size:
2053 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1688 2209 2887 3161 3308 3719 1688
Size: 521 678 274 147 411 2468
Fragments arranged by size:
2468 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1421 1451 1421
Size: 30 4469
BseYI C'CCAG_C
Cuts at: 1420 1924 2682 3259 3755 1420
Size: 504 758 577 496 2164
Fragments arranged by size:
2164 758 577 504 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1471 1471
Size: 4499
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3295 104
Size: 1937 46 1208 1308
Fragments arranged by size:
1937 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1246 1513 2291 2481 3273 1246
Size: 267 778 190 792 2472
Fragments arranged by size:
2472 792 778 267 190
Bsp1286I G_dGCh'C
Cuts at: 522 1246 1421 1460 1513 2124 2217 2291 2481
Size: 724 175 39 53 611 93 74 190
Cuts at: 2481 2543 3273 522
Size: 62 730 1748
Fragments arranged by size:
1748 730 724 611 190 175 93 74
62 53 39
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1549 1763 2065 2446 1549
Size: 214 302 381 3602
Fragments arranged by size:
3602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 392 2411 392
Size: 2019 2480
BsrFI r'CCGG_y
Cuts at: 2497 2678 2887 2497
Size: 181 209 4109
Fragments arranged by size:
4109 209 181
BssSI C'ACGA_G
Cuts at: 2770 3128 2770
Size: 358 4141
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 110 2860 110
Size: 2750 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 997 997
Size: 4499
BstYI r'GATC_y
Cuts at: 1 1432 1524 1991 2014 2349 2595 3607 3693
Size: 1431 92 467 23 335 246 1012 86
Cuts at: 3693 3705 4051 1
Size: 12 346 449
Fragments arranged by size:
1431 1012 467 449 346 335 246 92
86 23 12
BtgI C'CryG_G
Cuts at: 125 277 125
Size: 152 4347
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 1431 1742 1431
Size: 311 4188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 156 191 156
Size: 35 4464
DraI TTT'AAA
Cuts at: 231 1498 231
Size: 1267 3232
DraIII CAC_nnn'GTG
Cuts at: 1285 1285
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 285 529 987 2084 2258 2649 2676 4021 285
Size: 244 458 1097 174 391 27 1345 763
Fragments arranged by size:
1345 1097 763 458 391 244 174 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 133 562 970 1391 2522 2732 133
Size: 429 408 421 1131 210 1900
Fragments arranged by size:
1900 1131 429 421 408 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2707 3017 3163 4309 2707
Size: 310 146 1146 2897
Fragments arranged by size:
2897 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1511 1244
Size: 267 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 383 623 2324 2756 3503 3771 335
Size: 48 240 1701 432 747 268 1063
Fragments arranged by size:
1701 1063 747 432 268 240 48
EcoO109I rG'GnC_Cy
Cuts at: 1638 1638
Size: 4499
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 343 2518 343
Size: 2175 2324
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 1933 2181 3203 4182 1933
Size: 248 1022 979 2250
Fragments arranged by size:
2250 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 769 801 1201 1233 2341 2371 2373 2403 769
Size: 32 400 32 1108 30 2 30 2865
Fragments arranged by size:
2865 1108 400 32 32 30 30 2
HincII GTy'rAC
Cuts at: 544 1538 544
Size: 994 3505
HindIII A'AGCT_T
Cuts at: 865 865
Size: 4499
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 124 342 448 3170 3354 124
Size: 218 106 2722 184 1269
Fragments arranged by size:
2722 1269 218 184 106
MscI TGG'CCA
Cuts at: 287 989 2260 287
Size: 702 1271 2526
Fragments arranged by size:
2526 1271 702
MslI CAynn'nnrTG
Cuts at: 995 2488 2615 995
Size: 1493 127 2879
Fragments arranged by size:
2879 1493 127
MspA1I CmG'CkG
Cuts at: 721 1610 1924 2284 3297 3542 721
Size: 889 314 360 1013 245 1678
Fragments arranged by size:
1678 1013 889 360 314 245
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NcoI C'CATG_G
Cuts at: 125 277 125
Size: 152 4347
NdeI CA'TA_TG
Cuts at: 560 560
Size: 4499
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NspI r_CATG'y
Cuts at: 1552 2052 2959 1552
Size: 500 907 3092
Fragments arranged by size:
3092 907 500
PciI A'CATG_T
Cuts at: 2955 2955
Size: 4499
PflMI CCAn_nnn'nTGG
Cuts at: 283 283
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PstI C_TGCA'G
Cuts at: 1546 2231 1546
Size: 685 3814
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 721 1924 2284 721
Size: 1203 360 2936
Fragments arranged by size:
2936 1203 360
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1246 1513 1246
Size: 267 4232
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SfcI C'TryA_G
Cuts at: 33 1049 1436 1445 1542 2227 3220 3411 33
Size: 1016 387 9 97 685 993 191 1121
Fragments arranged by size:
1121 1016 993 685 387 191 97 9
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 328 1654 2569 3061 3323 328
Size: 1326 915 492 262 1504
Fragments arranged by size:
1504 1326 915 492 262
SnaBI TAC'GTA
Cuts at: 1491 1491
Size: 4499
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1552 2052 1552
Size: 500 3999
SspI AAT'ATT
Cuts at: 1081 1081
Size: 4499
StyI C'CwwG_G
Cuts at: 125 277 1164 1460 1633 1987 125
Size: 152 887 296 173 354 2637
Fragments arranged by size:
2637 887 354 296 173 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1273 2082 2215 2674 2883 1273
Size: 809 133 459 209 2889
Fragments arranged by size:
2889 809 459 209 133
TatI w'GTAC_w
Cuts at: 177 1056 4014 177
Size: 879 2958 662
Fragments arranged by size:
2958 879 662
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 854 886 1424 1456 4016 4048 854
Size: 32 538 32 2560 32 1305
Fragments arranged by size:
2560 1305 538 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1120 2296 1120
Size: 1176 3323
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1994 1994
Size: 4499
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
XmnI GAAnn'nnTTC
Cuts at: 686 806 686
Size: 120 4379
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI
SwaI XhoI ZraI