Restriction map for
pF1KSDA1831
(Circular) (Six-base) MAPSORT of: pF1KSDA1831.seq Check: 5622 from: 1 to: 5021
pF1KSDA1831 5021 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AatII G_ACGT'C
Cuts at: 825 825
Size: 5021
AccI GT'mk_AC
Cuts at: 2059 2059
Size: 5021
Acc65I G'GTAC_C
Cuts at: 2037 2037
Size: 5021
AclI AA'CG_TT
Cuts at: 4861 4861
Size: 5021
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 2846 3278 4025 4293 418
Size: 2428 432 747 268 1146
Fragments arranged by size:
2428 1146 747 432 268
AfeI AGC'GCT
Cuts at: 1021 1021
Size: 5021
AflIII A'CryG_T
Cuts at: 2373 3477 4454 2373
Size: 1104 977 2940
Fragments arranged by size:
2940 1104 977
AgeI A'CCGG_T
Cuts at: 3409 3409
Size: 5021
AhdI GACnn_n'nnGTC
Cuts at: 741 741
Size: 5021
AleI CACnn'nnGTG
Cuts at: 964 964
Size: 5021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1180 1180
Size: 5021
AlwNI CAG_nnn'CTG
Cuts at: 248 1371 2326 3893 248
Size: 1123 955 1567 1376
Fragments arranged by size:
1567 1376 1123 955
ApaI G_GGCC'C
Cuts at: 223 293 725 920 1700 1800 223
Size: 70 432 195 780 100 3444
Fragments arranged by size:
3444 780 432 195 100 70
ApaLI G'TGCA_C
Cuts at: 213 1138 3791 213
Size: 925 2653 1443
Fragments arranged by size:
2653 1443 925
ApoI r'AATT_y
Cuts at: 2025 4374 4630 2025
Size: 2349 256 2416
Fragments arranged by size:
2416 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5021
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
AvaI C'yCGr_G
Cuts at: 133 690 698 724 1854 1891 2041 133
Size: 557 8 26 1130 37 150 3113
Fragments arranged by size:
3113 1130 557 150 37 26 8
BamHI G'GATC_C
Cuts at: 1492 2046 1492
Size: 554 4467
BanI G'GyrC_C
Cuts at: 226 505 1222 1874 2037 2451 2699 2734 226
Size: 279 717 652 163 414 248 35 2513
Fragments arranged by size:
2513 717 652 414 279 248 163 35
BanII G_rGCy'C
Cuts at: 223 293 452 697 725 809 920 955 1439
Size: 70 159 245 28 84 111 35 484
Cuts at: 1439 1561 1700 1781 1800 1823 1849 1892 1901
Size: 122 139 81 19 23 26 43 9
Cuts at: 1901 2035 3065 223
Size: 134 1030 2179
Fragments arranged by size:
2179 1030 484 245 159 139 134 122
111 84 81 70 43 35 28 26
23 19 9
BbeI G_GCGC'C
Cuts at: 230 1226 2455 2703 230
Size: 996 1229 248 2548
Fragments arranged by size:
2548 1229 996 248
BbsI GAAGACnn'nnnn_
Cuts at: 273 1741 273
Size: 1468 3553
BbvCI CC'TCA_GC
Cuts at: 1667 1792 1975 1667
Size: 125 183 4713
Fragments arranged by size:
4713 183 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 987 1021 1900 1934 987
Size: 34 879 34 4074
Fragments arranged by size:
4074 879 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2919 3680 5021 2919
Size: 761 1341 2919
Fragments arranged by size:
2919 1341 761
BclI T'GATC_A
Cuts at: 2541 2541
Size: 5021
BglI GCCn_nnn'nGGC
Cuts at: 1097 1336 1097
Size: 239 4782
BglII A'GATC_T
Cuts at: 2536 4573 2536
Size: 2037 2984
BlpI GC'TnA_GC
Cuts at: 799 2133 799
Size: 1334 3687
Bme1580I G_kGCm'C
Cuts at: 198 217 223 258 293 569 725 920 1142
Size: 19 6 35 35 276 156 195 222
Cuts at: 1142 1700 1800 2646 2739 3795 198
Size: 558 100 846 93 1056 1424
Fragments arranged by size:
1424 1056 846 558 276 222 195 156
100 93 35 35 19 6
BmrI ACTGGGnnnn_n'
Cuts at: 283 2233 2314 2649 4793 283
Size: 1950 81 335 2144 511
Fragments arranged by size:
2144 1950 511 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 510 3388 510
Size: 2878 2143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1578 1592 1578
Size: 14 5007
Bpu10I CC'TnA_GC
Cuts at: 1667 1792 1817 1975 1667
Size: 125 25 158 4713
Fragments arranged by size:
4713 158 125 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2197 3076 3568 3866 2197
Size: 879 492 298 3352
Fragments arranged by size:
3352 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 481 4725 481
Size: 4244 777
BsaAI yAC'GTr
Cuts at: 171 2013 2374 171
Size: 1842 361 2818
Fragments arranged by size:
2818 1842 361
BsaBI GATnn'nnATC
Cuts at: 6 2535 4572 6
Size: 2529 2037 455
Fragments arranged by size:
2529 2037 455
BsaHI Gr'CG_yC
Cuts at: 227 278 640 731 822 1010 1223 1286 2452
Size: 51 362 91 91 188 213 63 1166
Cuts at: 2452 2700 3402 4898 227
Size: 248 702 1496 350
Fragments arranged by size:
1496 1166 702 362 350 248 213 188
91 91 63 51
BsaWI w'CCGG_w
Cuts at: 889 2210 2731 3409 3683 3830 4241 889
Size: 1321 521 678 274 147 411 1669
Fragments arranged by size:
1669 1321 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1102 1132 1102
Size: 30 4991
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1123 1512 1630 1708 1771 1774 1993 1123
Size: 389 118 78 63 3 219 4151
Fragments arranged by size:
4151 389 219 118 78 63 3
BseYI C'CCAG_C
Cuts at: 179 251 342 387 1053 1507 1678 1800 1870
Size: 72 91 45 666 454 171 122 70
Cuts at: 1870 1922 1997 2446 3204 3781 4277 179
Size: 52 75 449 758 577 496 923
Fragments arranged by size:
923 758 666 577 496 454 449 171
122 91 75 72 70 52 45
BsiEI CG_ry'CG
Cuts at: 104 770 926 2563 2609 3817 104
Size: 666 156 1637 46 1208 1308
Fragments arranged by size:
1637 1308 1208 666 156 46
BsiHKAI G_wGCw'C
Cuts at: 217 1142 1892 2035 2813 3003 3795 217
Size: 925 750 143 778 190 792 1443
Fragments arranged by size:
1443 925 792 778 750 190 143
BsmI GAATG_Cn'
Cuts at: 1984 1984
Size: 5021
BsmBI CGTCTCn'nnnn_
Cuts at: 1697 1697
Size: 5021
Bsp1286I G_dGCh'C
Cuts at: 198 217 223 258 293 452 569 697 725
Size: 19 6 35 35 159 117 128 28
Cuts at: 725 809 920 955 1142 1439 1561 1700 1781
Size: 84 111 35 187 297 122 139 81
Cuts at: 1781 1800 1823 1849 1892 1901 2035 2646 2739
Size: 19 23 26 43 9 134 611 93
Cuts at: 2739 2813 3003 3065 3795 198
Size: 74 190 62 730 1424
Fragments arranged by size:
1424 730 611 297 190 187 159 139
134 128 122 117 111 93 84 81
74 62 43 35 35 35 28 26
23 19 19 9 6
BspEI T'CCGG_A
Cuts at: 4241 4241
Size: 5021
BspHI T'CATG_A
Cuts at: 4197 4197
Size: 5021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1379 2071 2285 2587 2968 1379
Size: 692 214 302 381 3432
Fragments arranged by size:
3432 692 381 302 214
BsrBI CCG'CTC
Cuts at: 50 814 3313 3367 4851 5016 50
Size: 764 2499 54 1484 165 55
Fragments arranged by size:
2499 1484 764 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2933 2933
Size: 5021
BsrFI r'CCGG_y
Cuts at: 769 1061 1130 3019 3200 3409 769
Size: 292 69 1889 181 209 2381
Fragments arranged by size:
2381 1889 292 209 181 69
BssHII G'CGCG_C
Cuts at: 984 984
Size: 5021
BssSI C'ACGA_G
Cuts at: 3292 3650 3292
Size: 358 4663
BstAPI GCAn_nnn'nTGC
Cuts at: 1380 2398 1380
Size: 1018 4003
BstBI TT'CG_AA
Cuts at: 110 3382 110
Size: 3272 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1944 1944
Size: 5021
BstYI r'GATC_y
Cuts at: 1 1492 2046 2513 2536 2871 3117 4129 4215
Size: 1491 554 467 23 335 246 1012 86
Cuts at: 4215 4227 4573 1
Size: 12 346 449
Fragments arranged by size:
1491 1012 554 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1613 1840 1613
Size: 227 4794
BtgI C'CryG_G
Cuts at: 375 540 1331 1958 375
Size: 165 791 627 3438
Fragments arranged by size:
3438 791 627 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1809 2971 3152 3315 1809
Size: 1162 181 163 3515
Fragments arranged by size:
3515 1162 181 163
BtsI GCAGTG_nn'
Cuts at: 1307 1320 1561 2264 1307
Size: 13 241 703 4064
Fragments arranged by size:
4064 703 241 13
DraI TTT'AAA
Cuts at: 2020 2020
Size: 5021
DrdI GACnn_nn'nnGTC
Cuts at: 1213 2727 3585 1213
Size: 1514 858 2649
Fragments arranged by size:
2649 1514 858
EaeI y'GGCC_r
Cuts at: 621 716 767 923 956 1349 2606 2780 3171
Size: 95 51 156 33 393 1257 174 391
Cuts at: 3171 3198 4543 621
Size: 27 1345 1099
Fragments arranged by size:
1345 1257 1099 393 391 174 156 95
51 33 27
EagI C'GGCC_G
Cuts at: 767 923 2606 767
Size: 156 1683 3182
Fragments arranged by size:
3182 1683 156
EarI CTCTTCn'nnn_
Cuts at: 1769 1880 3044 3254 1769
Size: 111 1164 210 3536
Fragments arranged by size:
3536 1164 210 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 311 788 833 3229 3539 3685 4831 311
Size: 477 45 2396 310 146 1146 501
Fragments arranged by size:
2396 1146 501 477 310 146 45
EcoICRI GAG'CTC
Cuts at: 1890 2033 1890
Size: 143 4878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1578 1592 2846 3278 4025 4293 418
Size: 1160 14 1254 432 747 268 1146
Fragments arranged by size:
1254 1160 1146 747 432 268 14
EcoNI CCTnn'n_nnAGG
Cuts at: 946 1294 1444 1571 946
Size: 348 150 127 4396
Fragments arranged by size:
4396 348 150 127
EcoO109I rG'GnC_Cy
Cuts at: 220 289 917 1368 1389 1585 1696 1796 1797
Size: 69 628 451 21 196 111 100 1
Cuts at: 1797 1861 2160 220
Size: 64 299 3081
Fragments arranged by size:
3081 628 451 299 196 111 100 69
64 21 1
EcoRI G'AATT_C
Cuts at: 2025 2025
Size: 5021
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 165 3040 165
Size: 2875 2146
FspI TGC'GCA
Cuts at: 859 2802 859
Size: 1943 3078
HaeII r_GCGC'y
Cuts at: 230 540 586 934 1023 1226 1257 2455 2703
Size: 310 46 348 89 203 31 1198 248
Cuts at: 2703 3725 4704 230
Size: 1022 979 547
Fragments arranged by size:
1198 1022 979 547 348 310 248 203
89 46 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 727 759 2863 2893 2895 2925 727
Size: 32 2104 30 2 30 2823
Fragments arranged by size:
2823 2104 32 30 30 2
HincII GTy'rAC
Cuts at: 2060 2060
Size: 5021
HindIII A'AGCT_T
Cuts at: 1604 1604
Size: 5021
KasI G'GCGC_C
Cuts at: 226 1222 2451 2699 226
Size: 996 1229 248 2548
Fragments arranged by size:
2548 1229 996 248
KpnI G_GTAC'C
Cuts at: 2041 2041
Size: 5021
MluI A'CGCG_T
Cuts at: 4454 4454
Size: 5021
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 1049 1451 3692 3876 521
Size: 528 402 2241 184 1666
Fragments arranged by size:
2241 1666 528 402 184
MscI TGG'CCA
Cuts at: 1351 2782 1351
Size: 1431 3590
MslI CAynn'nnrTG
Cuts at: 964 1828 3010 3137 964
Size: 864 1182 127 2848
Fragments arranged by size:
2848 1182 864 127
MspA1I CmG'CkG
Cuts at: 245 365 377 410 803 914 1001 1270 1791
Size: 120 12 33 393 111 87 269 521
Cuts at: 1791 1903 2132 2446 2806 3819 4064 245
Size: 112 229 314 360 1013 245 1202
Fragments arranged by size:
1202 1013 521 393 360 314 269 245
229 120 112 111 87 33 12
NaeI GCC'GGC
Cuts at: 771 3202 771
Size: 2431 2590
NarI GG'CG_CC
Cuts at: 227 1223 2452 2700 227
Size: 996 1229 248 2548
Fragments arranged by size:
2548 1229 996 248
NgoMIV G'CCGG_C
Cuts at: 769 3200 769
Size: 2431 2590
NspI r_CATG'y
Cuts at: 2074 2574 3481 2074
Size: 500 907 3614
Fragments arranged by size:
3614 907 500
PasI CC'CwG_GG
Cuts at: 231 834 1456 231
Size: 603 622 3796
Fragments arranged by size:
3796 622 603
PciI A'CATG_T
Cuts at: 3477 3477
Size: 5021
PflMI CCAn_nnn'nTGG
Cuts at: 1607 1607
Size: 5021
PfoI T'CCnGG_A
Cuts at: 467 1569 1967 467
Size: 1102 398 3521
Fragments arranged by size:
3521 1102 398
PmeI GTTT'AAAC
Cuts at: 2020 2020
Size: 5021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 4160
Size: 32 4989
PpuMI rG'GwC_Cy
Cuts at: 1368 1585 1861 1368
Size: 217 276 4528
Fragments arranged by size:
4528 276 217
PshAI GACnn'nnGTC
Cuts at: 1583 1583
Size: 5021
PspOMI G'GGCC_C
Cuts at: 219 289 721 916 1696 1796 219
Size: 70 432 195 780 100 3444
Fragments arranged by size:
3444 780 432 195 100 70
PspXI vC'TCGA_Gb
Cuts at: 1854 1854
Size: 5021
PstI C_TGCA'G
Cuts at: 1376 1732 2068 2753 1376
Size: 356 336 685 3644
Fragments arranged by size:
3644 685 356 336
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5021
PvuII CAG'CTG
Cuts at: 2446 2806 2446
Size: 360 4661
RsrII CG'GwC_CG
Cuts at: 892 3216 892
Size: 2324 2697
SacI G_AGCT'C
Cuts at: 1892 2035 1892
Size: 143 4878
SacII CC_GC'GG
Cuts at: 378 378
Size: 5021
SalI G'TCGA_C
Cuts at: 2058 2058
Size: 5021
SanDI GG'GwC_CC
Cuts at: 1585 1585
Size: 5021
SapI GCTCTTCn'nnn_
Cuts at: 1769 1880 3044 3254 1769
Size: 111 1164 210 3536
Fragments arranged by size:
3536 1164 210 111
SbfI CC_TGCA'GG
Cuts at: 2068 2068
Size: 5021
ScaI AGT'ACT
Cuts at: 4538 4538
Size: 5021
SfcI C'TryA_G
Cuts at: 33 1372 1728 2064 2749 3742 3933 33
Size: 1339 356 336 685 993 191 1121
Fragments arranged by size:
1339 1121 993 685 356 336 191
SfoI GGC'GCC
Cuts at: 228 1224 2453 2701 228
Size: 996 1229 248 2548
Fragments arranged by size:
2548 1229 996 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5021
SmaI CCC'GGG
Cuts at: 135 700 726 2043 135
Size: 565 26 1317 3113
Fragments arranged by size:
3113 1317 565 26
SmlI C'TyrA_G
Cuts at: 1854 2176 3091 3583 3845 1854
Size: 322 915 492 262 3030
Fragments arranged by size:
3030 915 492 322 262
SnaBI TAC'GTA
Cuts at: 2013 2013
Size: 5021
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5021
SphI G_CATG'C
Cuts at: 2074 2574 2074
Size: 500 4521
StuI AGG'CCT
Cuts at: 1917 1917
Size: 5021
StyI C'CwwG_G
Cuts at: 2155 2509 2155
Size: 354 4667
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1862 2604 2737 3196 3405 1862
Size: 742 133 459 209 3478
Fragments arranged by size:
3478 742 459 209 133
TatI w'GTAC_w
Cuts at: 239 1118 4536 239
Size: 879 3418 724
Fragments arranged by size:
3418 879 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4538 4570 4538
Size: 32 4989
Tth111I GACn'n_nGTC
Cuts at: 2818 2818
Size: 5021
XbaI T'CTAG_A
Cuts at: 2052 2052
Size: 5021
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1830 1944 2516 1830
Size: 114 572 4335
Fragments arranged by size:
4335 572 114
XhoI C'TCGA_G
Cuts at: 1854 1854
Size: 5021
XmaI C'CCGG_G
Cuts at: 133 698 724 2041 133
Size: 565 26 1317 3113
Fragments arranged by size:
3113 1317 565 26
ZraI GAC'GTC
Cuts at: 823 823
Size: 5021
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AloI AscI AvrII BaeI BfrBI BmgBI
BmtI BsgI BsiWI BsrGI BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PsiI PsrI
SexAI SfiI SgrAI SrfI SspI SwaI XmnI