Restriction map for
pF1KSDA1835
(Circular) (Six-base) MAPSORT of: pF1KSDA1835.seq Check: 8698 from: 1 to: 4898
pF1KSDA1835 4898 bp
With 182 enzymes: SgfI *
June 20, 2008 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 909 909
Size: 4898
AatII G_ACGT'C
Cuts at: 1427 1427
Size: 4898
AccI GT'mk_AC
Cuts at: 1886 1936 1886
Size: 50 4848
Acc65I G'GTAC_C
Cuts at: 1914 1914
Size: 4898
AclI AA'CG_TT
Cuts at: 4738 4738
Size: 4898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1132 2723 3155 3902 4170 691
Size: 441 1591 432 747 268 1419
Fragments arranged by size:
1591 1419 747 441 432 268
AfeI AGC'GCT
Cuts at: 367 367
Size: 4898
AflII C'TTAA_G
Cuts at: 480 480
Size: 4898
AflIII A'CryG_T
Cuts at: 383 1511 2250 3354 4331 383
Size: 1128 739 1104 977 950
Fragments arranged by size:
1128 1104 977 950 739
AgeI A'CCGG_T
Cuts at: 3286 3286
Size: 4898
AhdI GACnn_n'nnGTC
Cuts at: 629 629
Size: 4898
AleI CACnn'nnGTG
Cuts at: 377 377
Size: 4898
AlwNI CAG_nnn'CTG
Cuts at: 1875 2203 3770 1875
Size: 328 1567 3003
Fragments arranged by size:
3003 1567 328
ApaI G_GGCC'C
Cuts at: 503 1478 503
Size: 975 3923
ApaLI G'TGCA_C
Cuts at: 1678 3668 1678
Size: 1990 2908
ApoI r'AATT_y
Cuts at: 1902 4251 4507 1902
Size: 2349 256 2293
Fragments arranged by size:
2349 2293 256
AseI AT'TA_AT
Cuts at: 20 932 20
Size: 912 3986
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
AvaI C'yCGr_G
Cuts at: 1522 1918 1522
Size: 396 4502
AvrII C'CTAG_G
Cuts at: 1470 1470
Size: 4898
BamHI G'GATC_C
Cuts at: 1923 1923
Size: 4898
BanI G'GyrC_C
Cuts at: 712 880 1914 2328 2576 2611 712
Size: 168 1034 414 248 35 2999
Fragments arranged by size:
2999 1034 414 248 168 35
BanII G_rGCy'C
Cuts at: 462 503 541 1478 1486 1912 2942 462
Size: 41 38 937 8 426 1030 2418
Fragments arranged by size:
2418 1030 937 426 41 38 8
BbeI G_GCGC'C
Cuts at: 716 884 2332 2580 716
Size: 168 1448 248 3034
Fragments arranged by size:
3034 1448 248 168
BbsI GAAGACnn'nnnn_
Cuts at: 143 269 913 1709 143
Size: 126 644 796 3332
Fragments arranged by size:
3332 796 644 126
BbvCI CC'TCA_GC
Cuts at: 1067 1319 1067
Size: 252 4646
BciVI GTATCCnnnnn_n'
Cuts at: 2796 3557 4898 2796
Size: 761 1341 2796
Fragments arranged by size:
2796 1341 761
BclI T'GATC_A
Cuts at: 2418 2418
Size: 4898
BglI GCCn_nnn'nGGC
Cuts at: 151 151
Size: 4898
BglII A'GATC_T
Cuts at: 2413 4450 2413
Size: 2037 2861
BlpI GC'TnA_GC
Cuts at: 461 540 1088 2010 461
Size: 79 548 922 3349
Fragments arranged by size:
3349 922 548 79
Bme1580I G_kGCm'C
Cuts at: 503 651 1478 1682 2523 2616 3672 503
Size: 148 827 204 841 93 1056 1729
Fragments arranged by size:
1729 1056 841 827 204 148 93
BmgBI CAC'GTC
Cuts at: 1514 1514
Size: 4898
BmrI ACTGGGnnnn_n'
Cuts at: 1391 2110 2191 2526 4670 1391
Size: 719 81 335 2144 1619
Fragments arranged by size:
2144 1619 719 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 4898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 721 775 1114 1180 1436 396
Size: 325 54 339 66 256 3858
Fragments arranged by size:
3858 339 325 256 66 54
Bpu10I CC'TnA_GC
Cuts at: 1067 1319 1067
Size: 252 4646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 550 1678 2074 2953 3445 3743 517
Size: 33 1128 396 879 492 298 1672
Fragments arranged by size:
1672 1128 879 492 396 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 892 1539 4602 892
Size: 647 3063 1188
Fragments arranged by size:
3063 1188 647
BsaAI yAC'GTr
Cuts at: 1890 2251 1890
Size: 361 4537
BsaBI GATnn'nnATC
Cuts at: 6 2412 4449 6
Size: 2406 2037 455
Fragments arranged by size:
2406 2037 455
BsaHI Gr'CG_yC
Cuts at: 713 881 1189 1424 2329 2577 3279 4775 713
Size: 168 308 235 905 248 702 1496 836
Fragments arranged by size:
1496 905 836 702 308 248 235 168
BsaWI w'CCGG_w
Cuts at: 635 2087 2608 3286 3560 3707 4118 635
Size: 1452 521 678 274 147 411 1415
Fragments arranged by size:
1452 1415 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 772 1228 1231 1234 1237 1276 1279 1297 1309
Size: 456 3 3 3 39 3 18 12
Cuts at: 1309 1312 1315 1318 1626 1663 772
Size: 3 3 3 308 37 4007
Fragments arranged by size:
4007 456 308 39 37 18 12 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 454 1499 2323 3081 3658 4154 454
Size: 1045 824 758 577 496 1198
Fragments arranged by size:
1198 1045 824 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 986 1615 1783 986
Size: 629 168 4101
Fragments arranged by size:
4101 629 168
BsiEI CG_ry'CG
Cuts at: 104 2440 2486 3694 104
Size: 2336 46 1208 1308
Fragments arranged by size:
2336 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 541 1486 1682 1912 2690 2880 3672 541
Size: 945 196 230 778 190 792 1767
Fragments arranged by size:
1767 945 792 778 230 196 190
BsmI GAATG_Cn'
Cuts at: 493 493
Size: 4898
BsmBI CGTCTCn'nnnn_
Cuts at: 1431 1431
Size: 4898
Bsp1286I G_dGCh'C
Cuts at: 462 503 541 651 1478 1486 1682 1912 2523
Size: 41 38 110 827 8 196 230 611
Cuts at: 2523 2616 2690 2880 2942 3672 462
Size: 93 74 190 62 730 1688
Fragments arranged by size:
1688 827 730 611 230 196 190 110
93 74 62 41 38 8
BspEI T'CCGG_A
Cuts at: 4118 4118
Size: 4898
BspHI T'CATG_A
Cuts at: 4074 4074
Size: 4898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 909 1948 2162 2464 2845 909
Size: 1039 214 302 381 2962
Fragments arranged by size:
2962 1039 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3190 3244 4728 4893 50
Size: 3140 54 1484 165 55
Fragments arranged by size:
3140 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 140 974 1718 2810 140
Size: 834 744 1092 2228
Fragments arranged by size:
2228 1092 834 744
BsrFI r'CCGG_y
Cuts at: 2896 3077 3286 2896
Size: 181 209 4508
Fragments arranged by size:
4508 209 181
BsrGI T'GTAC_A
Cuts at: 1877 1877
Size: 4898
BssSI C'ACGA_G
Cuts at: 1675 3169 3527 1675
Size: 1494 358 3046
Fragments arranged by size:
3046 1494 358
BstAPI GCAn_nnn'nTGC
Cuts at: 235 1609 2275 235
Size: 1374 666 2858
Fragments arranged by size:
2858 1374 666
BstBI TT'CG_AA
Cuts at: 110 3259 110
Size: 3149 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 723 1185 1437 723
Size: 462 252 4184
Fragments arranged by size:
4184 462 252
BstYI r'GATC_y
Cuts at: 1 410 605 1923 2390 2413 2748 2994 4006
Size: 409 195 1318 467 23 335 246 1012
Cuts at: 4006 4092 4104 4450 1
Size: 86 12 346 449
Fragments arranged by size:
1318 1012 467 449 409 346 335 246
195 86 23 12
BtgI C'CryG_G
Cuts at: 125 884 890 990 1073 125
Size: 759 6 100 83 3950
Fragments arranged by size:
3950 759 100 83 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 315 2848 3029 3192 315
Size: 2533 181 163 2021
Fragments arranged by size:
2533 2021 181 163
BtsI GCAGTG_nn'
Cuts at: 224 716 800 1598 2141 224
Size: 492 84 798 543 2981
Fragments arranged by size:
2981 798 543 492 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 561 596 561
Size: 35 4863
DraI TTT'AAA
Cuts at: 1897 1897
Size: 4898
DraIII CAC_nnn'GTG
Cuts at: 1761 1761
Size: 4898
DrdI GACnn_nn'nnGTC
Cuts at: 1512 2604 3462 1512
Size: 1092 858 2948
Fragments arranged by size:
2948 1092 858
EaeI y'GGCC_r
Cuts at: 179 887 893 1175 2483 2657 3048 3075 4420
Size: 708 6 282 1308 174 391 27 1345
Cuts at: 4420 179
Size: 657
Fragments arranged by size:
1345 1308 708 657 391 282 174 27
6
EagI C'GGCC_G
Cuts at: 2483 2483
Size: 4898
EarI CTCTTCn'nnn_
Cuts at: 1229 1238 1253 1274 1286 1307 2921 3131 1229
Size: 9 15 21 12 21 1614 210 2996
Fragments arranged by size:
2996 1614 210 21 21 15 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3106 3416 3562 4708 3106
Size: 310 146 1146 3296
Fragments arranged by size:
3296 1146 310 146
EcoICRI GAG'CTC
Cuts at: 539 1484 1910 539
Size: 945 426 3527
Fragments arranged by size:
3527 945 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 691 721 775 1114 1132 1180 1436 2723
Size: 295 30 54 339 18 48 256 1287
Cuts at: 2723 3155 3902 4170 396
Size: 432 747 268 1124
Fragments arranged by size:
1287 1124 747 432 339 295 268 256
54 48 30 18
EcoNI CCTnn'n_nnAGG
Cuts at: 757 811 757
Size: 54 4844
EcoO109I rG'GnC_Cy
Cuts at: 525 749 1474 1785 2037 525
Size: 224 725 311 252 3386
Fragments arranged by size:
3386 725 311 252 224
EcoRI G'AATT_C
Cuts at: 1902 1902
Size: 4898
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4898
FspI TGC'GCA
Cuts at: 2679 2679
Size: 4898
HaeII r_GCGC'y
Cuts at: 369 716 884 1750 1832 2332 2580 3602 4581
Size: 347 168 866 82 500 248 1022 979
Cuts at: 4581 369
Size: 686
Fragments arranged by size:
1022 979 866 686 500 347 248 168
82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 337 369 615 647 2740 2770 2772 2802 337
Size: 32 246 32 2093 30 2 30 2433
Fragments arranged by size:
2433 2093 246 32 32 30 30 2
HincII GTy'rAC
Cuts at: 828 1937 828
Size: 1109 3789
HindIII A'AGCT_T
Cuts at: 731 731
Size: 4898
KasI G'GCGC_C
Cuts at: 712 880 2328 2576 712
Size: 168 1448 248 3034
Fragments arranged by size:
3034 1448 248 168
KpnI G_GTAC'C
Cuts at: 1918 1918
Size: 4898
MluI A'CGCG_T
Cuts at: 4331 4331
Size: 4898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 335 1676 3569 3753 335
Size: 1341 1893 184 1480
Fragments arranged by size:
1893 1480 1341 184
MscI TGG'CCA
Cuts at: 889 1177 2659 889
Size: 288 1482 3128
Fragments arranged by size:
3128 1482 288
MslI CAynn'nnrTG
Cuts at: 377 2887 3014 377
Size: 2510 127 2261
Fragments arranged by size:
2510 2261 127
MspA1I CmG'CkG
Cuts at: 192 454 465 992 2009 2323 2683 3696 3941
Size: 262 11 527 1017 314 360 1013 245
Cuts at: 3941 192
Size: 1149
Fragments arranged by size:
1149 1017 1013 527 360 314 262 245
11
NaeI GCC'GGC
Cuts at: 3079 3079
Size: 4898
NarI GG'CG_CC
Cuts at: 713 881 2329 2577 713
Size: 168 1448 248 3034
Fragments arranged by size:
3034 1448 248 168
NcoI C'CATG_G
Cuts at: 125 890 1073 125
Size: 765 183 3950
Fragments arranged by size:
3950 765 183
NgoMIV G'CCGG_C
Cuts at: 3077 3077
Size: 4898
NspI r_CATG'y
Cuts at: 387 771 1951 2451 3358 387
Size: 384 1180 500 907 1927
Fragments arranged by size:
1927 1180 907 500 384
PasI CC'CwG_GG
Cuts at: 1122 1122
Size: 4898
PciI A'CATG_T
Cuts at: 383 3354 383
Size: 2971 1927
PflMI CCAn_nnn'nTGG
Cuts at: 778 778
Size: 4898
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 554 586 4037 4069 554
Size: 32 3451 32 1383
Fragments arranged by size:
3451 1383 32 32
PpuMI rG'GwC_Cy
Cuts at: 749 749
Size: 4898
PspOMI G'GGCC_C
Cuts at: 499 1474 499
Size: 975 3923
PstI C_TGCA'G
Cuts at: 231 560 618 1422 1871 1945 2630 231
Size: 329 58 804 449 74 685 2499
Fragments arranged by size:
2499 804 685 449 329 74 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4898
PvuII CAG'CTG
Cuts at: 192 454 465 2323 2683 192
Size: 262 11 1858 360 2407
Fragments arranged by size:
2407 1858 360 262 11
RsrII CG'GwC_CG
Cuts at: 404 3093 404
Size: 2689 2209
SacI G_AGCT'C
Cuts at: 541 1486 1912 541
Size: 945 426 3527
Fragments arranged by size:
3527 945 426
SacII CC_GC'GG
Cuts at: 993 993
Size: 4898
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4898
SanDI GG'GwC_CC
Cuts at: 749 749
Size: 4898
SapI GCTCTTCn'nnn_
Cuts at: 1307 2921 3131 1307
Size: 1614 210 3074
Fragments arranged by size:
3074 1614 210
SbfI CC_TGCA'GG
Cuts at: 560 1945 560
Size: 1385 3513
ScaI AGT'ACT
Cuts at: 4415 4415
Size: 4898
SfcI C'TryA_G
Cuts at: 33 227 556 614 1418 1867 1941 2626 3619
Size: 194 329 58 804 449 74 685 993
Cuts at: 3619 3810 33
Size: 191 1121
Fragments arranged by size:
1121 993 804 685 449 329 194 191
74 58
SfoI GGC'GCC
Cuts at: 714 882 2330 2578 714
Size: 168 1448 248 3034
Fragments arranged by size:
3034 1448 248 168
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
SmaI CCC'GGG
Cuts at: 1920 1920
Size: 4898
SmlI C'TyrA_G
Cuts at: 480 532 565 1693 2053 2968 3460 3722 480
Size: 52 33 1128 360 915 492 262 1656
Fragments arranged by size:
1656 1128 915 492 360 262 52 33
SnaBI TAC'GTA
Cuts at: 1890 1890
Size: 4898
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4898
SphI G_CATG'C
Cuts at: 1951 2451 1951
Size: 500 4398
StuI AGG'CCT
Cuts at: 340 340
Size: 4898
StyI C'CwwG_G
Cuts at: 125 335 651 722 890 959 1073 1178 1470
Size: 210 316 71 168 69 114 105 292
Cuts at: 1470 1781 2032 2386 125
Size: 311 251 354 2637
Fragments arranged by size:
2637 354 316 311 292 251 210 168
114 105 71 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 640 2481 2614 3073 3282 421
Size: 219 1841 133 459 209 2037
Fragments arranged by size:
2037 1841 459 219 209 133
TatI w'GTAC_w
Cuts at: 1877 4413 1877
Size: 2536 2362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 625 630 657 662 4415 4447 625
Size: 5 27 5 3753 32 1076
Fragments arranged by size:
3753 1076 32 27 5 5
Tth111I GACn'n_nGTC
Cuts at: 1708 2695 1708
Size: 987 3911
XbaI T'CTAG_A
Cuts at: 1929 1929
Size: 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 174 1830 2393 174
Size: 1656 563 2679
Fragments arranged by size:
2679 1656 563
XmaI C'CCGG_G
Cuts at: 1918 1918
Size: 4898
XmnI GAAnn'nnTTC
Cuts at: 1145 1627 1145
Size: 482 4416
ZraI GAC'GTC
Cuts at: 1425 1425
Size: 4898
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AlfI AloI AscI BaeI BcgI BfrBI BmtI BsaXI
BsiWI BssHII BstEII BstZ17I Bsu36I ClaI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SexAI
SfiI SgrAI SrfI SspI SwaI XhoI